特应性皮炎分子遗传学研究进展

特应性皮炎的分子遗传学研究近年来取得了一定进展 ,通过基因组扫描和遗传连锁分析已在人类 1、3、5、13、17、2 0号染色体上发现了该病的易感区域。其与 HL A的相关性研究早已开展 ,寻找该病候选基因正是目前研究的热点     

特应性皮炎分子遗传学研究进展

特应性皮炎的分子遗传学研究近年来取得了一定进展，通过基因组扫描和遗传连锁分析巳在人类1、3、5、13、17、20号染色体上发现了该病的易感区域。其与HLA的相关性研究早巳开展，寻找该病候选基因正是目前研究的热点。     

白细胞介素31与特应性皮炎

IL-31是由Th2细胞分泌的一种新型细胞因子,它能结合由IL-31BA和制瘤素M受体(OSMR)组成的异二聚体型Ⅰ类细胞因子受体进行信号传导.IL-31及其受体mRNA水平的高低与不同的组织及刺激条件有关,其生物学活性与皮肤病理及炎性细胞募集有关,在特应性皮炎(AD)的发生和发展中发挥着重要的作用.     

特应性皮炎的研究进展

特应性皮炎(atopic dermatitis,AD)是一种常见的儿童过敏性皮肤病,具有明显的遗传倾向,常伴有剧烈瘙痒,易并发哮喘和过敏性鼻炎,严重影响患者的生活质量.大量的研究表明,皮肤屏障、皮肤微生态和皮肤免疫微环境的相互作用共同促进了AD的炎症进程,这些研究为治疗这种难治性皮肤病提供了多种新靶标和新思路.因此,本...     

趋化因子及其受体与特应性皮炎

趋化因子及其受体在许多疾病的病理生理过程中起着重要作用,是当前医学科研领域的一个热点。近年来趋化因子及其受体在特应性皮炎（AD）中的研究显示CC型趋化因子、CX3C型趋化因子、CXC型趋化因子及其受体与AD的发病机制、临床表现、SCORAD评分、病情活动、治疗反应、治疗监测等关系密切,这对探讨AD发病机制和探索新的治疗方案具有重要意义。     

IL-10基因多态性与特应性皮炎发生的相关性

目的 研究白细胞介素-10（IL-10）基因启动子区域的单核苷酸多态性与特应性皮炎发生的相关性。方法 门诊特应性皮炎患儿174名和健康儿童130名,利用Taqman方法分析IL-10基因启动子区域 -1082（G/A）, -819（C/T）, -592(C/A) 单核苷酸多态性和单核苷酸组合成的单倍型和基因型。分析特定基因型保留和血液中嗜酸性粒细胞总数或血浆中IgE浓度之间的关联性。结果 健康组儿童ACC单倍型频率显著高于特应性皮炎儿童（p﹤0.05）。而血液中嗜酸性粒细胞总数和血浆中IgE浓度,ATA/ATA基因型的儿童显著高于ATA/ACC基因型儿童（p﹤0.05）。结论 IL-10基因启动子区域的单核苷酸多态性与特应性皮炎发生有关。     

29例特应性皮炎患者血清总IgE水平的检测

文本用双抗体夹心酶联免疫吸附试验(ELISA)测定了21例无过敏史和寄生虫卵检查阴性的健康对照及29例临床确诊为特应性皮炎(AD)患者的血清总IgE水平。结果表明:(1)AD病人血清总IgE水平明显高于正常对照,经统计学处理差别非常显著(P<0.01);(2)IgE水平与临床症状有呈正相关的倾向,症状严重者IgE水平较高,反之较低,但其差别未达到统计学上有意义的程度;(3)IgE水平与家族过敏史的相关性不明显,具有家族过敏史的AD病人其血清总IgE水平较无家族史者高,但无统计学意义;IgE水平与个人过敏史的关系亦不明显;(4)並非所有AD患者的血清总IgE水平都高于对照组的几何均值,对其可能的原因进行了分析。     

健康教育对儿童特应性皮炎的干预效果

目的 探讨健康教育对儿童特应性皮炎（AD）的干预效果。方法 选择我院2017年5月~8月门诊收治的120例儿童AD患儿,按时间先后顺序分为实验组和对照组,每组60例。实验组给予常规治疗并给予相关知识健康教育,定期电话回访。对照组给予常规治疗,定期电话回访。比较两组入组时、入组1年后AD病情严重程度评分（SCORAD评分）、家长护理依从性、AD家庭影响调查问卷（DFI）评分。结果 入组1年后与对照组比较,实验组SCORAD评分为（3.28±0.87）分,家长护理依从性评分为（13.10±4.12）分,DFI评分为（6.82±2.15）分,均优于对照组的（6.23±1.23）分、（8.38±3.19）分和（10.38±2.47）分,差异具有统计学意义（P＜0.05）。结论 儿童AD患儿在常规治疗基础上实施健康教育,可促使家长了解疾病相关知识,提高治疗依从性,从而促使疗效提高,降低因疾病对家庭的影响。     

特应性皮炎全基因组关联研究进展北大核心CSCD

随着测序与分析技术的发展,近年来特应性皮炎(AD)的全基因组关联研究(GWAS)逐渐增加。本文系统地总结了AD的GWAS,发现自2009年以来共报道了11个AD的GWAS,以欧洲人群居多,仅见1个在中国汉族和1个在日本人群的报告。这些GWAS共发现36个染色体区域上76个SNPs与AD在全基因组水平上相关。相关基因主要与表皮皮肤屏障功能和免疫功能相关。这些发现为AD的精准分型和治疗带来新的视角。     

粉尘螨浸液治疗特应性皮炎的疗效和IgE变化的观察

<正> 生物素-抗生物素蛋白酶联吸附试验(BA-ELISA)。 近年来,应用脱敏治疗过敏性疾病已取得了一定疗效,其中包括用粉尘螨治疗特应性皮炎(Atopic Dermatitis,AD),但多因疗程较短,未能发现显著性免疫学变化。我们选择AD患者分别用粉尘螨脱敏治疗和常规药物治疗。历经3年的临床疗效观察和测定血清IgE及特异性IgE水平,并与正常成人分析对比,结果如下。     

Allergens in Atopic Dermatitis

Allergens play an essential role in atopic dermatitis, either intrinsic or extrinsic. They provoke cutaneous inflammation via IgE-dependent and cell-mediated immune reactions. Food allergens have a well-known contribution to disease activity of atopic dermatitis, especially in infants and young children. However, the importance of inhaled allergens is still under investigation. For clinical implication, identification of individualized allergens is an ideal strategy for better control of atopic dermatitis and avoidance of atopic march. The aim of this article is to discuss the common allergens in atopic dermatitis (AD), the specificity and sensitivity of laboratory tests for allergens, and the clinical effect of various preventions.     

The Genetics of Atopic Dermatitis

Background Atopic dermatitis (AD) is a complex genetic disorder influenced by environmental factors. The mode of inheritance and genes involved are not clear. Results This report here is focusing on the current progress in searching the disease-susceptibility genes of AD via both the linkage studies and candidate gene approaches. Genome-wide linkage studies have identified multiple susceptibility loci on 3q and 17q. Candidate region linkage studies identify other susceptibility loci on 5q23–33, 11q13, and 13q12–14. At least 28 candidate genes have to date been verified in association studies, but only association with genes of interleukin (IL)-4, IL-13, IL-4RA, mast cell chymase, and serine protease inhibitor, kazal-type 5 have been replicated in more than two different studies. More halpotype tests and family-based association studies may help to shed more light for the candidate gene approach. Conclusion Determining the candidate susceptibility genes for AD is not only helping understanding the pathophysiology but also affecting the response to therapy, which is important in pharmacogenetics. The effect of environmental trigger may also have to be considered to elucidate the real face of the disease.     

Introduction of the Reliable Estimation of Atopic Dermatitis in ChildHood: Novel,Diagnostic Criteria for Childhood Atopic Dermatitis

PurposeQuestionnaire-based diagnostic criteria for atopic dermatitis (AD) have been proposed to detect the major group of AD with flexural dermatitis. We aimed to develop novel, questionnaire-based diagnostic criteria for childhood AD, which can detect more comprehensive AD including non-flexural type.MethodsThe draft version of questionnaire to detect childhood AD was prepared to be used for preliminary hospital- (n=1,756) and community-based (n=1,320) surveys. From analysis, the Reliable Estimation of Atopic dermatitis of ChildHood (REACH) was derived and verified in derivation (n=1,129) and validation (n=1,191) sets by community-based surveys.ResultsThe REACH consists of 11 questions including 2 major and 9 minor criteria. AD is diagnosed as the major group of ''eczema on the antecubital or popliteal fossa'' to fulfill the 2 major criteria (2M), and the minor group of ''eczema on the non-antecubital or popliteal fossa'' to fulfill the 1 major plus 4 or more minor criteria (1M+4m). In the validation set, the overall 1-year AD prevalence by the REACH was estimated as 12.3% (95% CI, 10.5%-14.2%), and the REACH showed a sensitivity of 75.2%, a specificity of 96.1%, and an error rate of 6.4%. The REACH demonstrated better diagnostic performance than the ISAAC in terms of the number of misclassification (10.0%).ConclusionsWe propose the REACH as new full, questionnaire-based diagnostic criteria for childhood AD in epidemiological surveys. Further studies are warranted to validate the REACH in different populations or countries in the context of large-scale, epidemiological surveys.     

Current Aspects of Innate and Adaptive Immunity in Atopic Dermatitis

Atopic dermatitis (AD) is a highly pruritic, chronic, multifactorial skin disease predisposing to bacterial and viral infections based on abnormalities of the innate and acquired immune system. The innate system quickly mobilizes an inflexible, standardized first-line response against different pathogens. Epidermal barrier dysfunction results in increased protein allergen penetration through the epidermis and predisposes to secondary skin infections. Two loss-of-function mutations in the epidermal filaggrin gene are associated with AD. Langerhans cells and inflammatory dendritic epidermal cells (IDEC) express high affinity IgE receptors, which are functional in IgE-mediated antigen presentation. Inducible antimicrobial peptides including the antiviral cathelicidin and the antibacterial beta-defensins show defective upregulation in lesional AD skin. The desmosomal protein nectin-1 is unmasked in AD lesions, thus becoming a relevant herpes simplex virus (HSV) entry receptor. Type I IFN-producing plasmacytoid dendritic cells are decreased and dysfunctional in AD skin, predisposing the patients to viral skin infections. Molluscum contagiosum virus produces a unique IL-18 binding protein to evade antiviral defense mechanisms. Innate and adaptive immunity do not simply coexist but are linked to one another in a complex network of skin immunobiology.