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Antioxidant activity of tea, content of bioactive compounds (rutin, quercetin, phenolic acids including: gallic, chlorogenic, protocatechuic, p-coumaric, caffeic, ferrulic, syringic and sinapic as well as other selected organic acids) and trace elements (Mn, selected as a metal playing a role in oxidative metabolism; Al; Cd; and Pb – the so-called heavy or noxious metals) were studied as these parameters greatly influence the quality of tea infusions. A number of different samples were tested including white, green, black and lemon-flavored teas, as well as the Red Lapacho tea. Antioxidant activity was measured using DPPH and ABTS tests, and a strong correlation between obtained results was observed. Infusions made from lemon teas packed in bags showed increased levels of rutin, quercetin and phenolic acids. Nevertheless, the positive effect of the increased level of phenolic compounds extracted is also connected with higher level of extracted noxious elements. Citric acid added to tea and herbal Red Lapacho infusions significantly increased Al, Cd, and Pb trace elements concentrations. Nevertheless, the infusions prepared from the lemon tea bags contained even the 10–70-fold higher content of these elements. On the other hand, addition of citric acid to tea infusions increased amount of extracted Mn only in black teas and Red Lapacho. 相似文献
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The variability in the fermentation index, polyphenols, and amino acids of rambutan seeds was investigated during fermentation. Results revealed that fermentation index (FI) value ≥1 was achieved on the 4th day of fermentation. While fermentation significantly reduced the levels of total polyphenols (59%), tannin (60%), and saponins (33%), it seems to have a moderate effect on geraniin, corilagin, and a much stronger effect on ellagic, and gallic acids. During fermentation, variability in gallic acid, geraniin, corilagin, and ellagic acid did not show a consistent trend. In contrast, amino acids significantly decreased up to the second day of fermentation. Importantly, amino acids (phenylalanine, tyrosine and leucine) with characteristic bitter taste showed reductions of 20%, 30%, and 40%, respectively after 10 days of fermentation. Sucrose, which was the only sugar present in significant concentrations in unfermented seed, was significantly reduced by fermentation. 相似文献
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Kye Man Cho Jin Hwan Lee Han Dae Yun Byung Yong Ahn Hoon Kim Weon Taek Seo 《Journal of food composition and analysis》2011,24(3):402-410
A potential probiotics Bacillus subtilis CS90 was isolated from Korean soybean paste (doenjang). Biotransformation of phytochemical contents, isoflavones, flavanols, and phenolic acids, during the fermentation of soybean fermented food cheonggukjang by a potential probiotics B. subtilis CS90 was investigated. The levels of isoflavone aglycones, flavanols, and gallic acid increased, while the β-glucosidase and esterase activities, isoflavone glycosides (except for acetylglycosides), and flavanol gallates contents decreased. Total isoflavone content slightly decreased after 60 h of fermentation, while total flavanol and phenolic acid content increased. The highest levels of daidzein (aglycone type) and acetyldaidzin (glycoside type) were recorded after 48 h of fermentation. However, the total contents of glycosides, manolylglycosides, and flavanol-gallates decreased by about 80, 90, and 70% during 60 h of fermenting cooked soybeans. In addition, the contents of total phenolic increased markedly from 253 mg/kg to 9414 mg/kg while the DPPH radical scavenging activity increased from 53.6 to 93.9% during this fermentation. 相似文献
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The purpose of this study was to investigate the effects of citrus pectin on human fecal neutral and acid steroid excretion and beta-glucuronidase and 7alpha-dehydroxylase activity. Eight healthy male subjects (age 20 to 27 yr) were used in a switchback design with or without 15 g citrus pectin added to a mixed low fiber diet. There were three successive 18-day periods preceded by a 4-day adjustment period. Half of the subjects followed a pectin-nonpectin-pectin protocol and the other half followed a nonpectin-pectin-nonpectin protocol. Fecal samples were collected throughout the study under anaerobic conditions. Compared to the control diet, mean fecal weight, percentage moisture, transit time, and fecal fat for both groups of subjects were not significantly different by analysis of variance when subjects were fed pectin diet. Mean beta-glucuronidase activity was increased (35%) when subjects were fed the pectin. Mean 7alpha-dehydroxylase activity showed no definite trend. Mean neutral steroid concentration was slightly decreased (8%) when the pectin diet was fed but total excretion was unchanged. End of period neutral steroid concentration was decreased 9% and total excretion was decreased 3.5%. Mean acid steroid concentration was not changed but total excretion was increased (11%) on the pectin diet. End of period acid steroid concentration and excretion was increased 6% on the pectin diet. This study shows that there were interrelationships between dietary pectin, neutral and acid steroid metabolism, and bacterial enzyme activity. 相似文献
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The aim of this study was to determine the amino acids major carotenoids and vitamin A activity in the 22 most frequently consumed traditional sauces in the Far North Region of Cameroon. Results showed that the most abundant amino acids in all sauces were glumatic acid (119–192 mg/g protein), aspartic acid (61.3–132 mg/g protein), leucine (34.5–99.2 mg/g protein) and phenylalanine (22.1–64.3 mg/g protein). High levels of amino acids were found in Gouboudou. Essential amino acids in most of the sauces represented up to 33% of total amino acids, indicating a good equilibrium between amino acids. Essential amino acids in most of these sauces met the recommended children requirement of the FAO/WHO/UNU for children 1–2 years old, except methionine and cysteine. Methionine + cysteine and lysine were the limiting amino acids in these sauces. Carotenoids and vitamin A activity (0.02–0.15 mg retinol activity equivalents/100 g dry weight) were present only in small quantities in all sauces when compared with other African sauces. 相似文献
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Some Viburnum species are used for preparation of the traditional drink called gilaburu in Anatolia. In the current study, our goal was to evaluate acetylcholinesterase (AChE) inhibitory and antioxidant activities of the ethyl acetate, methanol, and water extracts prepared from the branches, leaves, and fruits of Viburnum opulus and Viburnum lantana along with salicin, amentoflavone, and chlorogenic acid, three major compounds abundantly found in these species. AChE enzyme inhibition was tested in vitro using an enzyme-linked immunosorbent assay microplate reader at 50, 100, and 200?μL/mL concentrations. Antioxidant activity was examined by ferrous ion chelating capacity, ferric reducing antioxidant power, and β-carotene bleaching assay at 500, 1,000, and 2,000?μg/mL. Total phenol and flavonoid contents of the extracts were also established by Folin-Ciocalteau and AlCl(3) reagents, respectively. Our data revealed that the leaf methanol extract of V. opulus displayed a significantly high inhibitory effect against AChE (57.63?±?1.23%, 87.41?±?0.99%, and 93.19?±?0.87% at 50, 100, and 200?μg/mL, respectively). The extracts of V. lantana exerted higher antioxidant activity. 相似文献
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1. The present work with growing rats was undertaken to study the effect of protein quality, gastrointestinal microbial activity and the level of nitrogen intake on protein utilization and energy digestibility. The experiment involved a total of thirty-six dietary treatments in a 9 x 4 factorial design, with five rats per treatment. The thirty-six diets resulted from nine protein sources. Each diet was composed of a basal N-free mixture plus minerals and vitamins, with N sources added at the expense of the N-free mixture to provide 15.0 gN/kg dry matter (DM) in the first three protein-addition treatments and 30.0 gN/kg DM in the fourth protein-addition treatment. The nine protein sources were soya-bean meal, casein, wheat gluten, skim-milk powder, meat-and-bone meal, wheat bran, barley, wheat and cooked brown beans (Phaseolus vulgaris). The four formulations for each protein source incorporated the protein unsupplemented at 15.0 gN/kg DM, unsupplemented at 30.0 gN/kg DM, or supplemented at 15.0 gN/kg DM with the estimated first-limiting amino acid or the antibiotic Nebacitin. 2. With all protein sources, the inclusion of the first-limiting amino acid had no effect on either protein or energy digestibility. 3. The microbial activity in the digestive tract affected protein utilization and energy digestibility to a different degree depending primarily on the level and type of dietary fibre. True protein digestibility (TD) of skim-milk powder and brown beans, both rich in easily-fermentable energy, increased from 0.959 to 1.000 and from 0.680 to 0.777 respectively by the addition of Nebacitin. TD of the other protein sources was only marginally affected by the antibiotic treatment. Only with brown beans was the biological value (BV) markedly affected by Nebacitin with an increase from 0.482 to 0.557 by the treatment. Energy digestibility was significantly lower in rats given antibiotic with soya-bean meal, wheat bran, barley, wheat and brown beans. 4. The effect of level of N intake on protein utilization was dependent on both protein quality and the fibre concentration of the diet. Protein sources with high BV were more affected than proteins of lower BV. It was concluded that TD is not always independent of dietary protein concentration. 相似文献
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de Souza AS Rocha MS Tavares do Carmo Md 《Nutrition (Burbank, Los Angeles County, Calif.)》2012,28(4):458-464
ObjectiveTo investigate whether dietary trans fatty acids (TFAs) are incorporated in the hippocampus and its effects on the growth and aversive and spatial memories of young rats.MethodsWistar rat offspring whose mothers were fed with normolipidic diets containing soybean oil (soy group) or hydrogenated vegetable oil (trans group) during gestation and lactation were used. Male and female pups received the same diets as their mothers until the end of behavioral testing. The composition of fatty acids in the total lipids of the diets and hippocampus was quantified by gas chromatography. The results were evaluated by Student’s t test or analysis of variance followed by the Bonferroni correction.ResultsThe trans male and female body weights were higher during lactation and after weaning, with trans males having the lower body weight of the two. There was incorporation of 0.11% and 0.17% of TFAs in the hippocampi of male and female rats, respectively. During passive avoidance test, there was no significant difference. In the water maze test, there was no significant difference between male groups in the training and retention phases, except on day 4, when there was a significant decrease in latency in trans males. Trans females were worse on day 2 only and showed an improvement in spatial memory during the probe trial.ConclusionThe TFAs were incorporated in small amounts in the hippocampus and did not affect aversive memory. However, spatial memory was modified in young rats fed with a diet rich in TFAs. These findings suggested that, in addition to the TFA content of the diet provided, it is important to consider the provision of essential fatty acids and the ω-6/ω-3 ratio. 相似文献
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V Wyss 《La Medicina del lavoro》1966,57(4):262-285