6种血液指标在类风湿关节炎中的诊断价值

目的探讨抗环瓜氨酸多肽抗体(抗CCP抗体)、类风湿因子(RF)、C反应蛋白(CRP)、红细胞沉降率(ESR)、血红蛋白(Hb)、血小板(PLT)血液指标在类风湿关节炎(RA)中的应用价值。方法选取2015年8月至2017年9月该院95例RA患者(RA组),同时选取61例非RA的风湿病患者(非RA风湿病组)和101例健康者(健康组)。分析比较各指标在不同分组中的水平,另外比较抗CCP抗体和RF检测在RA中的诊断价值。结果 RA组的抗CCP抗体、RF、ESR、CRP、PLT水平高于健康组,且差异有统计学意义(P0.05);Hb水平低于健康组,差异有统计学意义(P0.05);RA组抗CCP抗体、RF、CRP、ESR、Hb、PLT水平与非RA风湿病组差异有统计学意义(P0.05)。抗CCP抗体诊断RA的灵敏度为88.42%,特异度99.38%,阳性预测值为98.82%,阴性预测值为93.60%,阳性似然比为142.610,阴性似然比为0.117。抗CCP抗体的ROC曲线下面积为0.942;RF诊断RA的灵敏度83.16%,特异度91.98%,阳性预测值为85.87%,阴性预测值为90.30%,阳性似然比为10.37,阴性似然比为0.183。两者联合检测的灵敏度更高[90.53%(86/95)]。RF的诊断RA的受试者工作特征曲线(ROC曲线)下面积为0.911。结论抗CCP抗体、RF是RA的特异性指标,在RA的诊断中抗CCP抗体价值优于RF,CRP、ESR、Hb、PLT指标不具有特异性但可以判断的RA活动度。     

电化学发光法检测抗环瓜氨酸肽抗体对类风湿关节炎的诊断价值

目的评价抗环瓜氨酸肽(CCP)抗体在类风湿关节炎(RA)诊断中的价值。方法选取2012年8月至2014年5月该院门诊及住院RA患者146例,其他非RA患者110例(其中非RA自身免疫性疾病患者48例,非自身免疫性疾病患者64例),同期体检健康者58例,采用电化学发光(ECLI)法和速率散射比浊分别检测其血清抗CCP抗体与类风湿因子(RF),分析抗CCP抗体与RF单项及联合检测的诊断效能。结果抗CCP抗体诊断RA的灵敏度、特异度、阳性预测值和阴性预测值分别为76.71%、98.20%、97.39%、82.59%,RF诊断RA的灵敏度、特异度、阳性预测值和阴性预测值分别为63.69%、84.02%、77.50%、72.96%;抗CCP抗体与RF联合检测的灵敏度、特异度、阳性预测值和阴性测值分别为57.53%、98.20%、96.55%、85.03%。结论采用ECLI法单独检测血清抗CCP抗体,具有灵敏度高、特异性强的特点,可以满足临床对RA的诊断要求,而联合RF只能提高RF对RA诊断的特异度、阳性预测值、阴性预测值,对抗CCP抗体的诊断效能无明显提升作用。     

抗环瓜氨酸肽抗体对类风湿关节炎的诊断价值

目的研究抗环瓜氨酸肽(cyclic citrullinated peptide,CCP)在类风湿性关节炎(rheumatoid arthritis,RA)诊断中的临床价值。方法收集确诊的RA50例,其他风湿性疾病患者50例及健康者50例,测定血清抗CCP抗体及类风湿因子(rheumatoid factor,RF)水平,应用四格表法计算抗CCP抗体诊断RA的敏感度、特异度、阳性预测值、阴性预测值,并与RF进行对比。结果抗CCP抗体在RA组阳性率最高;诊断RA,抗CCP抗体的敏感度和特异度、阳性预测值、阴性预测值为74.0%、97.0%、92.5%、88.2%;RF为86.0%、58.0%、50.6%、89.2%。结论抗CCP抗体诊断RA有较高的特异度,可用于RA的诊断与鉴别诊断。     

联合检测抗CCP抗体和RF在诊断类风湿关节炎中的意义

目的探讨联合检测抗环瓜氨酸肽(抗CCP抗体)和类风湿因子(RF)在诊断类风湿关节炎(RA)中的意义。方法RA患者65例,其他自身免疫性疾病组47例,健康对照组50例,采用胶乳增强免疫比浊法检测抗CCP抗体和RF的水平,统计比较对RA诊断的敏感性、特异性、阳性预测值,阴性预测值。结果 RA组中的RF的特异性为83.51%,抗CCP抗体的特异性为94.85%,联合检测特异性为95.88%,RA组中的RF阳性预测值为75.38%,抗CCP抗体的阳性预测值为85.80%,联合检测阳性预测值为90.91%,经χ2检验明显高于其他自身免疫性疾病组和健康对照组(P0.01)。结论联合检测抗CCP抗体和RF可以提高类风湿关节炎诊断的特异性和阳性预测值,有助于其早期诊断,具有较高的临床价值。     

抗突变型瓜氨酸波形蛋白抗体对类风湿关节炎的诊断价值

目的探讨抗突变型瓜氨酸波形蛋白(mutated citrullinated vimentin,MCV)抗体对类风湿关节炎(rheumatoid arthritis,RA)的临床诊断价值。方法 791例RA患者为RA组,1 369例非RA自身免疫病患者为非RA组,100例体检健康者为对照组,3组采用ELISA法检测血清抗MCV抗体和抗环瓜氨酸多肽(cyclic citrullinated peptide,CCP)抗体水平,分析抗MCV和抗CCP抗体的相关性及其诊断RA的敏感性和特异性。结果 RA组血清抗MCV抗体[(95.1±16.6)RU/mL]和抗CCP抗体水平[(89.3±12.9)RU/mL]明显高于非RA组[(8.8±4.3)、(17.5±5.8)RU/mL](P0.05)和对照组[(6.8±2.9)、(9.1±3.5)RU/mL](P0.05),非RA组与对照组比较差异无统计学意义(P0.05);ROC曲线分析结果显示,抗MCV抗体及抗CCP抗体诊断RA的AUC均为0.95;抗MCV抗体诊断RA的敏感性为82.1%,特异性为97.9%,阳性预测值为95.4%,阴性预测值为91.1%;抗CCP抗体诊断RA的敏感性为78.1%,特异性为98.1%,阳性预测值为94.5%,阴性预测值为90.3%;抗MCV抗体与抗CCP抗体呈正相关(r=0.726,P=0.008)。结论抗MCV抗体与抗CCP抗体诊断RA均有较好的敏感性和特异性,抗MCV抗体敏感性高于抗CCP抗体,可作为诊断RA的有效指标。     

抗环瓜氨酸肽抗体与类风湿性关节炎骨侵蚀相关性研究

目的：评价抗环瓜氨酸肽抗体（抗CCP抗体）和类风湿因子（IgM-RF）在类风湿性关节炎（RA）诊断中的意义及与骨侵蚀性的相关性.方法：选取类风湿性关节炎106例,其他疾病对照组273例（其中非RA的风湿性疾病132例,非风湿性疾病91例,正常人群50例）.对上述379份血清进行抗CCP抗体及IgM-RF检测,同时对106例RA患者行手足X摄片,将其按修正的Sharp评分分为有骨侵蚀组与无骨侵蚀组,并分析抗CCP抗体、IgM-RF及两者联合检测的敏感度、特异度、阳性似然比和阴性似然比及与骨侵蚀的相关性.结果：抗CCP抗体阳性者与RA骨侵蚀有相关性（P＜0.01）,IgM-RF阳性与骨侵蚀无相关性（P＞0.05）.抗CCP抗体及IgM-RF对诊断RA的敏感度、特异度、阳性似然比和阴性似然比分别为：抗CCP抗体（66.04%,98.90%,60.09,0.34）,IgM-RF（74.53%,79.49%,3.63,0.32）.抗CCP抗体与IgM-RF联合检测敏感度和特异度分别为58.49%、99.27%,阳性似然比和阴性似然比分别为83.56、0.42.提示抗CCP抗体特异度、阳性似然比明显高于IgM-RF,IgM-RF较抗CCP抗体有较高的敏感度,两者联合检测可提高诊断的特异度和阳性似然比、阴性似然比,但不能提高敏感度.结论：抗CCP抗体阳性者存在严重的骨关节破坏,IgM-RF与骨侵蚀无相关性.抗CCP抗体对RA诊断特异度高,IgM-RF特异度相对较低,两者联合检测可提高诊断的敏感度、特异度及阳性似然比、阴性似然比,但不能提高敏感度.抗CCP抗体对RA预后价值需进一步研究.     

国产抗环瓜氨酸肽抗体试剂盒在类风湿关节炎诊断中的应用价值

目的检测类风湿关节炎(RA)患者以及非RA的自身免疫性疾病患者血清中环瓜氨酸肽(CCP)抗体,探讨国产抗CCP抗体试剂盒在RA诊断中的敏感性和特异性。方法用酶联免疫吸附试验(ELISA)分别检测209例RA患者,82例非RA的其他自身免疫性疾病患者以及154名正常体检者的抗CCP抗体。结果以正常体检者组为对照,RA患者组抗CCP抗体的敏感性为72.25%,特异性为98.7%,阳性预测值为98.7%,阴性预测值为72.3%。以非RA自身免疫性疾病患者组为对照,RA患者组抗CCP抗体的敏感性为72.25%,特异性为76.83%,阳性预测值为88.8%,阴性预测值为52.0%。结论该抗CCP抗体的试剂盒对RA的诊断具有较高的敏感性和特异性,且操作简便,成本较低,有较高的临床应用价值。     

自身抗体检测在类风湿关节炎的临床诊断价值评价

目的研究类风湿因子(RF),抗环瓜氨酸肽(CCP)抗体,抗角蛋白抗体(AKA)三种自身抗体联合检测在类风湿关节炎(RA)中的临床诊断价值。方法抗CCP抗体采用酶联免疫吸附实验,AKA采用间接免疫荧光法,RF采用速率散射比浊法。检测240例病人血清中的RF,抗CCP抗体和AKA。结果 RF,抗CCP抗体和AKA三个特异性指标在RA组阳性率分别为78.7%,77.3%,63.4%明显高于非RA组,差异有统计学意义(P<0.05)。RF,抗CCP抗体和AKA在类风湿关节炎的诊断中灵敏度分别为78.7%、77.3%、63.3%,特异度为77.8%、92.2%、98.8%,阳性预测值85.5%、94.3%、98.9%,阴性预测值68.6%、70.9%、61.8%,阳性似然比3.55、9.91、52.75,阴性似然比0.27、0.25、0.37,诊断符合率78.3%、82.9%、76.7%。将三者串联检测的灵敏度和特异度为59.3%和98.9%。三者并联检测的灵敏度和特异度为87.3%和70.0%。结论在RA的诊断中RF,抗CCP抗体和AKA有较高的特异性和灵敏度,尤其是抗CCP抗体的诊断评价指标最好。三个指标串联检测可以提高特异度减少误诊率,三个指标并联检测可以提高灵敏度降低漏诊率,因此三个指标联合检测指导临床对RA的早期诊断具有重要价值。     

抗环瓜氨酸肽抗体和血管内皮生长因子对类风湿关节炎实验诊断意义探讨

目的探讨抗环瓜氨酸肽(CCP)抗体和血管内皮生长因子(VEGF)在类风湿关节炎(RA)诊断中的意义以及与类风湿因子(RF)的相关性。方法采用酶联免疫吸附试验(ELISA)分别检测实验组和对照组抗CCP抗体和VEGF,采用速率散射光比浊法检测RF水平,并分析上述三者之间的关系。其中实验组为48例RA患者,对照组为32名健康人。结果抗CCP抗体特异性、敏感性、阳性预测值和阴性预测值依次为100.0%、85.4%、100.0%、82.1%;VEGF的特异性、敏感性、阳性预测值和阴性预测值依次为56.3%、97.9%、77.0%、94.7%;RF特异性、敏感性、阳性预测值和阴性预测值依次为100.0%、89.6%、100.0%、86.5%。三者之间两两具有相关性,尤以VEGF和抗CCP抗体相关性为好。结论抗CCP抗体和VEGF在RA诊断中具有其独特的应用价值。抗CCP抗体特异性与RF相同并优于VEGF,VEGF敏感性最高,而三者联合检测可进一步提高试验诊断特异性和敏感性。     

抗CCP抗体、RA33抗体在类风湿性关节炎诊断中的表达

目的探讨抗环瓜氨酸肽(CCP)抗体、RA33抗体对类风湿性关节炎(RA)的临床诊断意义。方法选择120例RA患者、115例非RA患者和40名健康体检者,应用酶联免疫吸附试验(ELISA)检测抗CCP抗体和抗RA33抗体,应用免疫透射比浊法检测RF。结果抗CCP抗体、RA33抗体和RF对RA诊断的敏感性分别为59.2%、33.3%、77.5%;特异性分别为98.7%、94.8%、76.8%,抗CCP抗体、RA33抗体对RA诊断的特异性明显高于RF(P<0.01)。阳性预测值分别为97.3%、83.3%、72.1%;阴性预测值分别为75.7%、64.8%、81.5%。结论抗CCP抗体、RA33抗体对RA诊断的特异性优于RF(P<0.01),可作为RA诊断的特异性血清学指标;RF与抗CCP抗体和RA33抗体同时检测用于RA的诊断是临床的最佳选择。     

自身免疫病患者血清中传染性非典型肺炎冠状病毒抗体阳性原因分析

目的 探讨传染性非典型肺炎又称严重急性呼吸综合征(SARS),冠状病毒(SARS-CoV)抗体在SARS病原学诊断中的特异性及其在系统性红斑狼疮(SLE)、类风湿性关节炎(RA)、干燥综合征(SS)和混合结缔组织病(MCTD)患者中的假阳性问题。方法 应用酶联免疫吸附试验(ELISA)和荧光定量RT-PCR技术检测了111名正常对照和58例SLE、20例RA、10例SS、16例MCTD患者做血清中SARS-CoV抗体的检测。结果 在111名正常对照中,IgM抗体均阴性,IgG抗体的阳性率为3.6％;IgG抗体诊断SARS的特异性为96.4％,两种抗体同时阳性诊断SARS的特异性为100％。58例SLE患者中,IgM抗体和IgG抗体阳性率分别为8.6％和32.8％,IgG抗体和IgM抗体同时阳性为19％;在10例SS患者中只有1例两种抗体同时阳性(10％);在16例MCTD患者中,IgG抗体阳性6例(37.5％),两种抗体同时阳性1例(6.3％);在20例RA患者中只有1例IgG抗体阳性(5％)。经RT-PCR检测,上述自身免疫病患者中的阳性病例血清SARS-CoV抗体均为阴性。结论用非纯化抗原制备的ELISA试剂盒测定自身免疫病患者的SARS-CoV抗体,可能出现假阳性,两种抗体同时测定可降低诊断的假阳性率,提高诊断的特异性。在自身免疫病患者中出现假阳性的原因可能与包被的抗原有关。     

CDC法与ELISA法检测RSA患者封闭抗体的实验诊断价值

目的探讨和比较补体依赖的淋巴细胞毒试验(CDC)与酶联免疫吸附试验(ELISA)检测反复自然流产患者(RSA)封闭抗体(BA)的诊断意义。方法运用CDC法和ELISA法对110名行淋巴细胞主动免疫治疗的RSA患者作BA检测,用kappa系数评价2种检测方法的一致性,用McNemar检验比较两者抗体阳性率的差异。结果CDC法检出的补体依赖型BA阳性率为9.09%(10/110);ELISA法检出的IgG型HLA抗体阳性率为41.82%(46/110)(P0.01),其中Ⅰ类抗体阳性、Ⅱ类抗体阳性、Ⅰ和Ⅱ类抗体均阳性者分别为6人、10人和30人。2种方法符合率为19.6%(9/46),kappa系数为0.202。ELISA法对HLA-Ⅰ和Ⅱ类抗体的检出率为32.7%(36/110)vs36.4%(40/110)(P0.05)。结论 CDC法和ELISA法检测封闭抗体的结果一致性较弱,ELISA法灵敏度明显高于CDC法。CDC法和ELISA法检测的BA不完全相同,各有其诊断意义。     

Detection of anti-nuclear antibodies from patients with systemic rheumatic diseases by elisa using hep-2 cell nuclei

A microtiter plate was coated with cell nuclei from HEp-2 cells, and the stabilized nuclei were fixed with acetone for an enzyme-linked immunosorbent assay (ELISA). Auto-antibodies against nuclear antigens were detected from the sera of patients with various systemic rheumatic disease but not from healthy individuals by means of the nucleus ELISA procedure. Ninety-one percent of anti-nuclear antibody (ANA)—positive sera as demonstrated by immunofluorescence (IF) test were also judged as positive for ANAs by the nucleus ELISA and 5% of them as psudo-positive. Patient's sera with homogeneous and fine-speckled IF patterns displayed the highest ELISA titers. A large portion of ss-A/Ro antigen is localized in the cytoplasm; therefore, anti-ss-A/Ro antibody was hardly detected by nucleus ELISA. The population of ANAs detectable by nucleus ELISA included anti-ss-B/La, anti-DNA, anti-histone, anti-Sm, anti-RNP, and anti-scl-70.     

LAV/HTLV-III antibodies in patients treated with intravenous gammaglobulins

The test sera from 50 patients treated with intravenous gammaglobulins (IVG) were examined for LAV/HTLV-III antibody by ELISA tests in order to evaluate the risk of transmission of LAV/HTLV-III virus via administration of IVG. The three ELISA-positive sera were negative for the antibody by both indirect immunofluorescence assay (IFA) and Western blot assay (WBA). Thus, all of the test sera were negative for LAV/HTLV-III antibody. Two of the ELISA-positive/IFA-negative/WBA-negative samples had HLA-DR4 antibodies causing positive ELISA results.     

Detection of cytomegalovirus-specific IGM in renal transplant recipients

We have compared two IgM-specific cytomegalovirus (CMV) antibody assays, an immunofluorescence assay (IFA-M) and an enzyme-linked antigen immunoassay (ELA-M), with an assay for CMV total antibody (ELISA) and viral culture for the detection of active CMV infection in renal transplant recipients. Of 75 patients (49 ELISA negative pretransplant, 26 ELISA positive), CMV-specific IgM was detected in 35 (27 ELISA negative pretransplant, 8 ELISA positive) using the IFA-M assay and in 25 (16 ELISA negative pretransplant, 9 ELISA positive) using the ELA-M test. Of the 25 patients identified as positive by ELA-M, 21 had positive viral cultures post-transplant, two seronegative patients had evidence of infection indicated by post-transplant seroconversion, and two patients were seropositive pretransplant but remained viral culture negative throughout the follow-up period. ELA-M and CMV total antibody ELISA detected primary infection in renal transplant recipients equally well, but ELA-M was found to be superior to ELISA and IFA-M for detecting reinfection and reactivation infections.     

不同方法联合检测SLE患者血清中双链DNA抗体的性能评估

目的探讨双链DNA抗体(dsDNA抗体)在常见自身免疫性疾病中的检出情况,评估不同方法联合检测系统性红斑狼疮(SLE)患者血清中dsDNA抗体的性能。方法使用ELISA法(ELISA1、ELISA2)和间接免疫荧光(IIF)法同时检测300例SLE和495例非SLE自身免疫疾病患者,以及300例健康体检者血清dsDNA抗体,分析单独和联合使用不同方法检测dsDNA抗体对SLE的诊断效能。结果 ELISA1、ELISA2和IIF在SLE患者中的检出率分别为42.3%、35.3%和38.0%,在非SLE自身免疫性疾病患者中最高检出率为1.2%,在健康体检人群中最高为0.3%。在SLE人群,3种方法中ELISA1和ELISA2法,ELISA1和IIF法,以及ELISA2和IIF法联合Kappa值分别为0.672、0.398和0.512(P0.05)。而只有ELISA1和ELISA2间的检测率差异有统计学意义(P=0.003)。ELISA1、ELISA2和IIF法检测dsDNA抗体的受试者工作特征曲线下面积(AUC)分别为0.708、0.672和0.687;ELISA1和IIF法的联合检出率最高为54.7%,AUC为0.764;3种方法同时检测,任何1种方法阳性即判断为阳性时,检出率为57.7%,AUC为0.781。结论联合使用ELISA法和IIF法,可以明显提升dsDNA抗体在SLE中的检出率。     

Screening for WBC antibodies by lymphocyte indirect immunofluorescence flow cytometry: superior to cytotoxicity and ELISA?

BACKGROUND: Cytotoxic WBC antibodies are found in patients who have refractoriness to platelet transfusion (RPT) or are experiencing febrile transfusion reactions (FTRs) and in sera giving so called nonspecific hemagglutination by IAT (N/S IAT). Sera from such patients were screened for WBC antibodies regardless of the ability to fix complement using a flow cytometric (FC) lymphocyte indirect immunofluorescence test (LIFT) to compare FC-LIFT with a routine lymphocytotoxicity test (LCT) for WBC antibody detection. STUDY DESIGN AND METHODS: Serum from 104 patients with RPT, 87 with FTR, and 147 with N/S IAT were tested in parallel by using FC-LIFT and LCT. Sera giving discrepant results were re-tested with an HLA class I antibody ELISA to assess whether they were HLA-specific. RESULTS: Of the sera tested, 175 were LIFT positive, and 146 were LCT positive. Fifty-five had antibodies that were detectable only by LIFT; 26 were positive only by LCT. Of these 81 discrepant sera, 30 of 63 were positive in HLA ELISA. CONCLUSION: FC-LIFT detects more WBC antibodies than does LCT or ELISA, and it is a superior screening technique. Because some cytotoxic antibodies are detectable only by LCT, comprehensive WBC antibody screening would require the application of both techniques. However, because FC assessments of cytotoxicity have been described, LCTs may become redundant for WBC antibody screening.     

Unexplained hepatitis C virus antibody seroconversion in established blood donors

BACKGROUND: Understanding of the epidemiology and natural history of hepatitis C virus (HCV) infection is incomplete without reference to the early phase of infection. The prevalence of HCV infection is well documented in numerous reports. The seroconversion pattern in previously antibody-negative blood donors provides a model for the study of the incidence and transmission of HCV infection. STUDY DESIGN AND METHODS: Records of HCV antibody tests at the West Midlands Blood Transfusion Centre were reviewed to determine the seroconversion rate in 1994 among previously anti-HCV-negative blood donors. Seroconverting donors were counseled to investigate the possible routes of infection. RESULTS: In 1994, blood donations (n = 256,935) were collected from 149,370 donors; 24 donors (0.016%; 1/6224) were positive in the screening enzyme-linked immunosorbent assay (ELISA) and the third- generation recombinant immunoblot assay (RIBA-3). Two donors previously negative for HCV antibody in ELISA were positive in both tests in 1994. Four donors positive in ELISA and indeterminate in RIBA-3 in 1993 reacted positively in both tests in 1994. One donor negative for HCV antibody on previous screening reacted positively in ELISA and was indeterminate in RIBA-3 in 1994 and has become positive in both tests in 1995. A further 43 donors negative for HCV antibody on previous screening reacted positively in ELISA and were indeterminate in RIBA-3 in 1994. CONCLUSION: Documented seroconversion can take place in the absence of exposure to recognizable risk factors for the infection. The index donation or the donation immediately preceding seroconversion may be positive for HCV RNA in the polymerase chain reaction.     

不同人类免疫缺陷病毒2型检测方法的比较研究

目的通过随访调查16例人类免疫缺陷病毒2型（HIV-2）可疑感染者，初步评价HIV一2的几种检测方法。方法从贵州省采集既往经HIV-1／2免疫印迹（WB）试验检测出现HIV-2指示带的16份受检者的全血样品，进行HIV-2抗体和核酸检测。血浆中的抗体用HIV-1／2ELISA初筛一复检，其中阳性样品再分别用HIV-1／2线性免疫试验（LIA）和HIV-2WB检测。外周血单核细胞（PBMC）中的前病毒DNA用HIV-2巢式PCR检测。结果16份血浆样品经HIV-1／2ELISA筛查均为HIV抗体阳性；用HIV-1／2 LIA检测，全部为HIV-1抗体阳性，15份为HIV-2抗体阴性、1份不确定；用HIV-2WB检测，有3份为HIV-2抗体阳性、13份不确定。由于这批样品的采样时间距首次检测至少1年以上，可以排除窗口期感染，上述检测结果不确定的受检者均可判为HIV-2抗体阴性。此外，用巢式PCR检测所有PBMC样品均为HIV一2核酸阴性。结论16例受检者全部为HIV-1而非HIV-2感染，HIV-1／2uA的抗体检测结果与HIV-2核酸检测结果基本相符，而HIV-2WB则产生了大量的不确定甚至假阳性结果。     

不同方法检测同种异基因造血干细胞移植受者巨细胞病毒感染的探讨

本研究探讨同种异基因造血干细胞移植（allo-HSCT）后早期有效检测巨细胞病毒（CMV）感染的方法。应用荧光定量PCR和ELISA试剂盒分别检测19名allo-HSCT受者,214份标本的血浆DNA负荷量和血清IgM抗体,同时应用流式细胞术检测188份标本白细胞pp65抗原。结果表明：pp65抗原、DNA定量和IgM抗体的阳性检出率分别为30.85%（58/188）、35.51%（76/214）和13.08%（28/214）,连续阳性病例和临床诊断的符合率分别为7/8、7/8和3/8。DNA定量与pp65抗原阳性检出率的差别无统计学意义（P〉0.05）,但两种检测方法有明显的相关性（P〈0.05）。IgM抗体阳性检出率明显低于DNA定量和pp65抗原,其差别均有统计学意义（P〈0.05）,与另两种检测方法虽有关系,但不密切。结论：流式细胞术和荧光定量PCR检测allo-HSCT受者CMV早期感染可靠、简便快速,值得临床推广使用。