VEGF-C、VEGFR-3在食管癌诊治中的临床价值

目的:探讨血管内皮细胞生长因子-C(vascular endothelial growth factor-C,VEGF-C)和血管内皮细胞生长因子受体-3(vascular endothelial growth factor receptor-3,VEGFR-3)在食管癌诊治中的临床价值.方法:收集2014年5月—20...     

补肾化痰祛瘀方治疗非痴呆性血管性认知功能障碍40例疗效观察

正血管性认知障碍(vascular cognitive impairment,VCI)是指由脑血管危险因素、显性或非显性脑血管病引起的从轻度认知损害到痴呆的一大类综合症[1]。一般将VCI分为3种临床亚型:非痴呆性血管性认知功能障碍(vascular cognitive impairment no dementia,VCIND)、血管性痴呆(vascular dementia,VaD)和伴有血管危险因素的混合型痴     

脉管性疾病592例临床分析

Background  Vascular anomalies are common and multidisciplinary involved diseases. The greatest impediment to their treatment in the past was their confusing terminology and clinical heterogeneities. This hospital-based retrospective study assessed some clinical characteristics, diagnosis, therapies and outcomes of patients with vascular anomalies in southeast China.

Methods  A total of 592 vascular anomalies patients (patients with intracranial tissues or viscera involved were excluded), admitted to the First Affiliated Hospital of Sun Yat-sen University from January 2006 to September 2009, were enrolled in the study. Data for clinical characteristics, diagnosis, therapies and outcomes were collected and analyzed.

Results  Of the 592 patients, the male:female ratios in the vascular tumor group (n=187) and the vascular malformation group (n=405) were 1:1.49 and 1:1.06 respectively, with no significant difference between them. The mean onset age of the vascular tumor group was significantly younger than that of the vascular malformation group (Ρ <0.001). The head and neck were the most commonly (31.4%) involved areas in vascular anomalies. A total of 23.8% of the patients with vascular anomalies had definite symptoms caused by the vascular lesions. In the vascular tumor group, 94.1% of them were infantile hemangiomas. Venous malformation was the most common (41.0%) subtype of vascular malformations. Surgical therapy was undertaken in 94.2% of the patients with vascular anomalies. Of the 519 patients available for the 16-58 month follow-up, 322 patients (62.0%) were cured, 108 patients (20.8%) were markedly improved, 57 patients (11.0%) were partially improved, and 32 patients (6.2%) were uncured.

Conclusions  Vascular anomalies are clinically heterogeneous. While the outcome is generally favorable, further effort should be made to determine the appropriate terminology and management.

     

Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro

Objective: To investigate the pro-angiogenic effects of paeoniflorin(PF) in a vascular insufficiency model of zebrafish and in human umbilical vein endothelial cells(HUVECs). Methods: In vivo, the pro-angiogenic effects of PF were tested in a vascular insufficiency model in the Tg(fli-1:EGFP)y1 transgenic zebrafish. The 24 h post fertilization(hpf) embryos were pretreated with vascular endothelial growth factor(VEGF) receptor tyrosine kinase inhibitor Ⅱ(VRI) for 3 h to establish the vascular insufficiency model and then post-treated with PF for 24 h. The formation of intersegmental vessels(ISVs) was observed with a fluorescence microscope. The m RNA expression of fms-like tyrosine kinase-1(flt-1), kinase insert domain receptor(kdr), kinase insert domain receptor like(kdrl) and von Willebrand factor(v WF) were analyzed by real-time polymerase chain reaction(PCR). In vitro, the pro-angiogenic effects of PF were observed in HUVECs in which cell proliferation, migration and tube formation were assessed. Results: PF(6.25–100 μmol/L) could rescue VRI-induced blood vessel loss in zebrafish and PF(25–100 μmol/L), thereby restoring the m RNA expressions of flt-1, kdr, kdrl and v WF, which were down-regulated by VRI treatment. In addition, PF(0.001–0.03 μmol/L) could promote the proliferation of HUVECs while PF stimulated HUVECs migration at 1.0–10 μmol/L and tube formation at 0.3 μmol/L. Conclusion: PF could promote angiogenesis in a vascular insufficiency model of zebrafish in vivo and in HUVECs in vitro.     

Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro

Objective: To investigate the pro-angiogenic effects of paeoniflorin (PF) in a vascular insufficiency model of zebrafish and in human umbilical vein endothelial cells (HUVECs). Methods: In vivo, the pro-angiogenic effects of PF were tested in a vascular insufficiency model in the Tg(fli-1:EGFP)y1 transgenic zebrafish. The 24 h post fertilization (hpf) embryos were pretreated with vascular endothelial growth factor (VEGF) receptor tyrosine kinase inhibitor Ⅱ (VRI) for 3 h to establish the vascular insufficiency model and then post-treated with PF for 24 h. The formation of intersegmental vessels (ISVs) was observed with a fluorescence microscope. The mRNA expression of fms-like tyrosine kinase-1 (flt-1), kinase insert domain receptor (kdr), kinase insert domain receptor like (kdrl) and von Willebrand factor (vWF) were analyzed by real-time polymerase chain reaction (PCR). In vitro, the pro-angiogenic effects of PF were observed in HUVECs in which cell proliferation, migration and tube formation were assessed. Results: PF (6.25–100 μmol/L) could rescue VRI-induced blood vessel loss in zebrafish and PF (25–100 μmol/L) , thereby restoring the mRNA expressions of flt-1, kdr, kdrl and vWF, which were down-regulated by VRI treatment. In addition, PF (0.001–0.03 μmol/L) could promote the proliferation of HUVECs while PF stimulated HUVECs migration at 1.0–10 μmol/L and tube formation at 0.3 μmol/L. Conclusion: PF could promote angiogenesis in a vascular insufficiency model of zebrafish in vivo and in HUVECs in vitro.     

抗VEGF药物联合激光治疗视网膜静脉阻塞的效果分析

目的:探讨对视网膜静脉阻塞患者在激光治疗的基础上联合抗血管内皮生长因子(vascular endothelial growth factor,VEGF)药物治疗的临床效果.方法:选择南通大学附属医院如皋分院2016年1月—2020年12月收治视网膜静脉阻塞患者40例,按照随机数表法分为常规激光治疗组(对照组)与联合抗V...     

Shexiang Baoxin Pill Regulates Intimal Hyperplasia,Migration, and Apoptosis after Platelet-Derived Growth Factor-BB-Stimulation of Vascular Smooth Muscle Cells via miR-451

Objective:To investigate the regulatory roles of Shexiang Baoxin Pill(SXBXW) in neointimal formation and vascular smooth muscle cells(VSMCs) invasion and apoptosis as well as the potential molecular mechanisms using cultured VSMCs model of vascular injury(platelet-derived growth factor(PDGF)-BBstimulated) in vitro.Methods:VSMCs were randomly assigned to 5 groups:blank,PDGF-BB(20 ng/mL+0.1%DMSO),SXBXW-L(PDGF-BB 20 ng/mL+SXBXW low dose 0.625 g/L),SXBXW-M(PDGF-BB 20 ng/mL+SXBXW medium dose 1.25 g/L...     

Comfortable lifestyle-induced imbalance of neuro-endocrineimmunity network: A possible mechanism of vascular endothelial dysfunction

<正>Objective:To observe the changes of vascular endothelial functions and general neuroendocrine -immunity(NEI) network under the state of qi-deficiency syndrome induced by excessive idleness and to approach their internal relevance and illuminate initially the pathophysiological mechanism of vascular lesion induced by excessive idleness.Methods:A total of 100 male Wistar rats were randomly divided into the control group and the qi-deficiency syndrome model group,50 rats in each group.The qi-deficiency syndrome model was established by feeding the animals with hyper-alimentation diet in combination with restricting movement for 10 weeks.Changes of common chemical signal molecules related to NEI and vascular endothelial functions were measured by the end of the experiment.Furthermore,their internal relevance was analyzed by the method of canonical correlation analysis.Results:The vascular endothelial structure and function were obviously injured in the model group.Compared with the control group,the chemical signal molecules,such as 5-hydroxytryptamine (5-HT),corticosterone(CORT),triiodothyronine(T3),tetraiodothyronine(T4),angiotensinⅡ(AngⅡ), interleukin-1(IL-1),and tumor necrosis factor-α(TNF-α) in peripheral blood of the model group(n=43) were changed significantly(P0.05 or P0.01).Canonical correlation analysis showed that vascular endothelial dysfunction was correlated to the changes of these signal molecules in the NEI network.Conclusions:Comfortbased lifestyle induced not only vascular endothelial dysfunction but also an imbalance of the NEI network. Vascular endothelial dysfunction and the imbalanced NEI network interacted with each other,and an imbalance of the NEI network may be the pathophysiologic basis for the genesis and development of vascular endothelial dysfunction,even diseases of the blood vessel.     

GRK2-YAP signaling is implicated in pulmonary arterial hypertension development

Background: Pulmonary arterial hypertension (PAH) is characterized by excessive proliferation of small pulmonary arterial vascular smooth muscle cells (PASMCs), endothelial dysfunction, and extracellular matrix remodeling. G protein-coupled receptor kinase 2 (GRK2) plays an important role in the maintenance of vascular tone and blood flow. However, the role of GRK2 in the pathogenesis of PAH is unknown.Methods: GRK2 levels were detected in lung tissues from healthy people and PAH patients. C57BL...     

Effect of Xuezhikang(血脂康)on Vascular Endothelial Function and Blood Level of Nitric Oxide and Endothelin in Patients with Hyperlipidemia

Objective:To study the effects of Xu-ezhikang(血脂康,XZK)on vascular endo-thelial function(VETF),serum nitric oxide(NO),plasma endothelin(ET)in patientswith hyperlipidemia.Methods:Fifty pa-     

子宫内膜癌细胞外泌体长链非编码RNA-C00473对人脐静脉内皮细胞VEGF和VEGFR-2表达的影响

目的 分析子宫内膜癌患者血清外泌体(exosome,Exo)中长链非编码RNA(lncRNA)C00473表达情况,及子宫内膜癌细胞(Ishikawa)外泌体对人脐静脉内皮细胞(human umbilical vascular endothelial cell,HUVECs)中血管内皮生长因子(vascular end...     

血管内皮细胞生长因子的家族成员及作用

血管内皮生长因子(vascular endothelial growth factor,VEGF)又称血管通透性因子(vascular permeability factor,VPF)或血管调理素(vasculotropin),是一组功能强大且能产生多种效应的细胞因子[1].     

Evaluation of spinal cord vessels using multi-slice CT angiography

Compared with the large number of head and neck vascular studies, computer tomography (CT) angiography of spinal vascular lesions has received relatively little attention. Several series and a few isolated cases of spinal vascular malformations demonstrated with magnetic resonance (MR) angiography have been reported. Selective digital subtraction angiography ( DSA ) is gold standard for diagnosis of spinal vascular lesions.However, it is invasive, time consuming, expensive, and dependent on the skills of the operator. Based on the DSA appearance and the surgical findings,     

血管内皮细胞生长因子表达调控及与非肿瘤疾病关系

血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)也称血管渗透因子(vascular permeability factor,VPF),最初有学者在研究癌性腹水时发现,肿瘤细胞可以分泌一种对肿瘤血管通透性产生刺激作用的因子,后来有研究于1989年从牛垂体滤泡星状细胞的条件培养基中提纯,     

血管内皮生长因子及其可溶性受体1在多囊卵巢综合征患者血清和卵巢组织的表达

目的:研究多囊卵巢综合征(Polycystic ovary syndrome,PCOS)患者与正常对照组血液和卵巢组织中血管内皮细胞生长因子(Vascular endothelial growth factor,VEGF)及其可溶性受体1(Soluble vascular endothelial growth fact...     

Evaluation of spinal cord vessels using multi-slice CT angiography

Compared with the large number of head and neck vascular studies, computer tomography (CT) angiography of spinal vascular lesions has received relatively little attention. Several series and a few isolated cases of spinal vascular malformations demonstrated with magnetic resonance (MR) angiography have been reported.1-3 Selective digital subtraction angiography (DSA) is gold standard for diagnosis of spinal vascular lesions.4 However, it is invasive, time consuming, expensive, and dependent on the skills of the operator. Based on the DSA appearance and the surgical findings, spinal vascular malformations are generally, although not universally, classified as intradural (intramedullary and/or extramedullary) arteriovenous malformation (AVM) or arteriovenous fistula (AVF), and dural AVF. Recommended treatments of dural AVF consist of surgical obliteration and/or embolization.5-8     

Efficacy and Effect of SI17 Therapy on Pancreatic Polypeptide in Vascular and Tension-Type Headache

Background and purpose:Vascular and tension-type headache is most commonly encountered,andSI17 therapy has been tested to treat headache with good results.The efficacy of SI17 therapy forvascular and tension-type headache was compared and the effect of SI17 therapy on pancreaticpolypeptide(PP)was studied.Materials and methods:29 cases of vascular headache(20 cases in acuteattack during the trial)and 27 cases of tension-type headache(19 cases in acute attack)were enrolledin the study.Plasma PP level before and 4th day after treatment was measured by radioimmunoassay.Results:SI17 therapy is better for the treatment of vascular headache.Vascular headache with higherPP level and tension-type headache with normal PP level had good therapeutic results.Conclusion:The clinical efficacy is better for vascular headache with the increase of vagus tension and for tension-type headache with normal vagus tension.     

低强度脉冲超声抑制血管平滑肌和成纤维细胞的钙化

目的:探究低强度脉冲超声(low intensity pulsed ultrasound,HPUS)对高钙磷诱导的大鼠血管平滑肌细胞(rat vascu-lar smooth muscle cell,rVSMC)及大鼠血管成纤维细胞(rat vascular adventitial fibroblasts,rVAF)钙...     

脑电图在血管性痴呆早期诊断中的应用分析

目的:观察血管性痴呆(vascular dementia,VD)患者的脑电图(electroencephalograph,EEG)变化,为EEG在VD早期诊断提供依据.方法:分析96例VD患者的EEG,同时与简易智能量表(Mini-Mental state Examination,MMSE)评分进行比较.结果:96例中...