HPLC法测定盐酸特拉唑嗪胶囊溶出度

目的:建立盐酸特拉唑嗪胶囊溶出度的高效液相色谱分析方法.方法:以C18(4.6×250mm,5μm)为色谱柱,甲醇-水-冰醋酸-三乙胺(500:500:10:0.3)为流动相,流速为1.0ml·min-1,检测波长为246nm.结果:盐酸特拉唑嗪在0.4～4.0μg·ml-1的浓度范围内,呈现良好线性相关(r=0.9995);平均回收率为99.5%,RSD为1.0%(n=9);三批样品测定结果符合规定.结论:采用本法测定盐酸特拉唑嗪胶囊溶出度,空白辅料峰与盐酸特拉唑嗪峰良好分离,有效消除空白胶囊与辅料干扰,且具有专属性强,灵敏度高,准确度好,简便快速等特点.     

高效液相色谱法测定盐酸特拉唑嗪及其胶囊中的有关物质

目的:建立高效液相色谱法测定盐酸特拉唑嗪及其胶囊剂中的有关物质.方法:采用色谱柱:DiamonsilC18色谱柱(150mm×4.6mm 5um);流动相:甲醇-水-冰醋酸-三乙胺(330:670:10:0.3);检测波长:246nm;流速:1.0ml·min-1.结果:原料药可检测出5个杂质峰,胶囊剂可检测出6个杂质峰.结论:本法简便,准确,灵敏度高,可用于盐酸特拉唑嗪及其胶囊剂中中有关物质的测定.     

进口和国产特拉唑嗪片人体生物等效性及临床费用-效果评价

评价国产和进口特拉唑嗪片在中国人群中的生物等效性及治疗前列腺增生的药物经济学.采用高效液相色谱-荧光检测法测定20名健康受试者口服2mg盐酸特拉唑嗪片后的血浆特拉唑嗪浓度;对128例前列腺增生患者分别用国产片剂和进口片剂进行治疗,运用药物经济学的费用-效果分析方法进行评估.国产盐酸特拉唑嗪和进口片剂具有生物等效性,国产特拉唑嗪是治疗前列腺增生的较为经济有效的口服药物.     

HPLC 法测定盐酸特拉唑嗪胶囊的含量和有关物质

目的:建立了HPLC 法测定盐酸特拉唑嗪胶囊的含量及有关物质.方法:色谱柱为Thermo C18柱(250×4.6mm,5μm),流动相:乙腈-三乙胺高氯酸溶液(取三乙胺2ml,置1000ml水中,用高氯酸调节pH2.0)(20:80),检测波长246nm,流速:1.0mL·min-1,柱温:40℃.结果:盐酸特拉唑嗪...     

高效液相色谱法与薄层色谱法测定盐酸特拉唑嗪片有关物质的比较

目的:通过用高效液相色谱法(HPLC)与薄层色谱法(TLC)测定盐酸特拉唑嗪片中有关物质的相关实验数据,确定精确可行、适宜应用的检测方法。方法:HPLC法,色谱柱为Krom asil C18(4.6 mm×250 mm,5μm),流动相为甲醇-水-冰醋酸-二乙胺(350∶650∶10∶0.3),流速为1 m l/m in,检测波长为246 nm。TLC法:以硅胶GF254薄层板展开,醋酸乙酯-甲醇-二乙胺(40∶0.5∶3)为展开剂。结果:用TLC法测定,盐酸特拉唑嗪最小检出限为0.025 mg/m l。用HPLC法测定,盐酸特拉唑嗪最低检测浓度为0.05μg/m l。结论:两种方法均可用于盐酸特拉唑嗪片中有关物质的测定。TLC法操作简单、方便。但HPLC法灵敏度更高,方法的最小检出限远低于TLC法,且定量更精确,适用于有关物质的检测。     

高效液相色谱法测定盐酸特拉唑嗪渗透泵控释片的含量

目的:建立盐酸特拉唑嗪渗透泵控释片的含量测定方法。方法:采用高效液相色谱法,色谱柱为ODS C18柱(150mm×4.6mm,5μm);流动相为甲醇-水-冰醋酸-二乙胺(35∶65∶1?0.02);检测波长:246nm。结果:盐酸特拉唑嗪在10.00~60.00mg.L-1之间,线性关系良好,r=0.999 5(n=6),平均回收率为99.67%,RSD为0.12%。结论:高效液相色谱法测定盐酸特拉唑嗪渗透泵控释片含量,无内源性物质的干扰,专属性强、灵敏度高,为该药质量控制提供参考依据。     

HPLC加校正因子的主成分自身对照法测定盐酸特拉唑嗪片中有关物质的含量

目的:建立测定盐酸特拉唑嗪片中有关物质含量的方法。方法:采用高效液相色谱加校正因子的主成分自身对照法。色谱柱为Agilent Zorbax Eclipse XDB C18,流动相为乙腈-高氯酸溶液(20∶80,V/V),流速为1.0 mL/min,检测波长为246 nm,进样量为20μL,柱温为50℃;绘制盐酸特拉唑嗪和杂质A、B、C的线性方程,以斜率计算各杂质相对于盐酸特拉唑嗪的校正因子,用相对保留时间确定各杂质位置,测定3批盐酸特拉唑嗪片中杂质A、B、C的含量,并与杂质对照品法测得的结果进行比较。结果:盐酸特拉唑嗪杂质A、B、C的相对保留时间分别为0.39、0.74、2.77,检测质量浓度线性范围均为0.25～3.0μg/mL,校正因子分别为0.75、1.09、0.84,检测限分别为0.35、0.51、0.43 ng,定量限分别为0.70、1.02、0.86 ng。3批盐酸特拉唑嗪片中杂质A、B、C的含量分别为0.11%～0.13%、0.03%、0.09%～0.12%,杂质B未检出;与杂质对照品法测定结果一致。结论:该方法简便快速,可准确测定盐酸特拉唑嗪片中有关物质的含量。     

高效液相色谱法测定人血清中盐酸特拉唑嗪

盐酸特拉唑嗪(terazosin)是α1肾上腺素受体阻断剂,用于治疗轻、中度高血压。本改进了测定血清中盐酸特拉唑嗪的高效液相色谱法,并测定了8名健康受试口服盐酸特拉唑嗪后的血药浓度。     

涂碳型PVC膜盐酸文拉法辛选择电极的研制与应用

目的:建立盐酸文拉法辛胶囊的测定方法.方法:用盐酸文拉法辛与四苯硼钠生成缔合物为电活性物,制做涂碳PVC膜盐酸文拉法辛电极.结果:在pH 4.0～6.5内,电极的Nernst响应范围为1.0×10-2～5.0×10-5 mol·L-1,斜率为57.0 mV/pc,检测限为2.0×10-5mol·L-1.结论:用于盐酸文拉法辛胶囊的含量测定,与电位滴定法基本一致.     

PVC膜离子选择电极测定盐酸地尔硫(艹卓)片

以盐酸地尔硫(艹卓)与四苯硼酸钠形成的缔合物为电活性物的聚氯乙烯(PVC)膜盐酸地尔硫(艹卓)选择电极,测定了盐酸地尔硫(艹卓)片的含量.电极的线性响应范围为1.0×1~(-5)~1.0×10~(-2)mol/L,级差电位为56mV/pc,检测限为3.2x10~(-6)mol/L.     

Electrode Material in Iontophoresis

Pharmaceutical Research -     

Electrode Material in Iontophoresis

Pharmaceutical Research -     

心电图电极

阐述生物医学信息检测电极的工作原理和电极噪声对心电图测量实验的影响。     

Ion-Selective Electrode for Piperazine Determination

Pharmaceutical Chemistry Journal -     

Electrode sensitive to sulfa drugs.

An electrode sensitive to sulfa drugs was constructed by using the iron(II)-bathophenanthroline chelate embedded in a liquid membrane. Rapid and Nernstian responses were exhibited against solutions of sulfamerazine and sulfisomidine ranging between 10(-3) and 10(-1) M in concentration. High selectivity was observed in the presence of urea, glycine, aminopyrine, or p-amino-benzoic acid. These chemicals are known to interfere in the usual colorimetric analysis of sulfa drugs.     

An Air-Gap Enzyme Electrode: Development and Applications

A novel air-gap enzyme electrode was developed by amalgamating the principles controlling ion-selective electrodes, enzyme kinetics, and diffusion of molecules across membranes. Commercially available ammonia and carbon dioxide gas-sensing electrodes were modified to measure the respective gases over a head-space. A plexiglass cell was designed and fabricated to house the modified electrodes. The air-gap electrodes exhibited superior sensitivity and response characteristics compared to the original membrane electrodes. The plexiglass cell was divided into two compartments by virtue of a selectively permeable membrane. Enzymatic reactions were conducted in the cell to determine quantitatively the concentration of the substrate. L-Phenylalanine ammonia lyase was used to determine L-phenylalanine with the ammonia electrode and L-glutamate decarboxylase was utilized for the determination of L-glutamic acid by the carbon dioxide electrode. Near-Nernstian slopes were obtained for the response of the enzyme electrodes. In addition to the good reproducibility, the method provided unique ability to reuse the same enzyme solution for several determinations of various concentrations of the analyte. The performance of the two compartment cell and air-gap enzyme electrode was found to be superior in comparison to conventional enzyme electrodes.     

硫酸双肼屈嗪在碳糊电极上的吸附伏安测定法的研究

目的:研究硫酸双肼屈嗪在碳糊电极上的电化学行为及检测方法。方法:在0.1molL-1氢氧化钠底液中,通过富集用碳糊电极吸附伏安法测定硫酸双肼屈嗪。结果:阴极峰电位为-0.224 V（vs.SCE）,峰电流与硫酸双肼屈嗪的浓度在2.8×10-7～39×10-5mol·L-1范围内呈良好的线性关系。该方法的检出下限为5.7×10-8mol·L-1。对常压片中硫酸双肼屈嗪含量用本法进行了测定,获得了满意的结果。结论:本方法的优点是成本低,操作方便,重现性好及检测下限较低。本文对电极响应机理进行了探讨,硫酸双肼屈嗪的电极过程是一个两电子两质子的准可逆过程。     

环丙沙星离子选择性电极的制备及应用

目的：制备一种以环丙沙星与四苯硼钠缔合物为活性物的PVC膜环丙沙星选择性电极。方法：用此电极以标准曲线法对盐酸环丙沙星及其两种制剂进行含量测定。结果：电极的响应范围10^-1～10^-5mol·L^-1，斜率为37．4mv／pC，平均回收率为97．7％，RSD=2．4％（n=8）。结论：方法灵敏度高，操作简便，结果与HPLC法相符。     

Ion-Selective Electrode for Determining 5-Nitrofuran Based Drugs

The construction and electroanalytical characteristics of ion-selective electrodes (ISEs) for the quantitative determination of furacilin, furadonin, and furagin are described. Ion association complexes of the drugs with N,N,N, N-tetradecylammonium were tested as electroactive materials for ionometric sensors. These ISEs show a linear response to drugs over a concentration range from 10⁻⁵ to 10⁻¹ mole/liter with anionic slopes from 51 to 58 mV/pC and possess low drug determination limits (∼5 × 10⁻⁶ mole/liter). The ISEs exhibit fast response, good stability, and reasonable selectivity. The proposed ISEs were successfully used for direct potentiometric analysis of furacilin, furadonin, and furagin in ready-to-use pharmaceutical preparations.__________Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 39, No. 2, pp. 39 – 41, February, 2005.     

聚氯乙烯吡咯烷酮膜离子选择性电极法测定左氧氟沙星的含量

目的:建立以聚乙烯吡咯烷酮膜离子选择性电极法测定左氧氟沙星含量的方法。方法:以左氧氟沙星碘化物与碘化铋的分子缔合物为电活性物质,研制了一种左氧氟沙星PVC膜涂层玻璃电极,并对其响应性能进行了系统研究。结果:该电极的Nernst响应范围为5.0×10-3~1.0×10-5moL.L-1,斜率为56.5mV.pC-1,适用pH范围为2.5~4.0,平均回收率为98.31%~101.6%(RSD=2.80%~4.90%)。结论:该电极法操作简单、响应迅速、重现性好,可用于左氧氟沙星的含量测定。