Therapeutic Effect of Chinese Medicinal Herbs Combined with Chemotherapy on Advanced Non-Small Cell Lung Cancer

Ｔｈｅｒａｐｅｕｔｉｃ　Ｅｆｆｅｃｔ　ｏｆ　Ｃｈｉｎｅｓｅ　Ｍｅｄｉｃｉｎａｌ　Ｈｅｒｂｓ　Ｃｏｍｂｉｎｅｄ　ｗｉｔｈ　Ｃｈｅｍｏｔｈｅｒａｐｙ　ｏｎ　Ａｄｖａｎｃｅｄ　Ｎｏｎ－Ｓｍａｌｌ　Ｃｅｌｌ　Ｌｕｎｇ　ＣａｎｃｅｒＨＵＡＮＧＺｈａｏ－ｍｉｎｇ...     

Short-term Effect of Hydroxyapatite Nanoparticles on Cancer Cells

Hydroxyapatite （HAP） is the main inorganic component of hard tissues and shows excellent biocompatibility and osteoconductivity properties. Nanoparticles of HAP can be synthesised by the precipitation method in distilled water. The needle shaped particles are below 100nm in size with low crystallinity and high surfacial activation. Recent studies have shown an inhibition effect of HAP nanoparticles on the proliferation of cancer cells. However, the effect of the HAP treatment over periods shorter than one day has not been reported. In this paper, the short-term effects of HAP nanoparticle sol on the proliferation of heptocellular carcinoma cells after 12 and 16 hours of treatment were investigated in vitro via MTT assay. The results revealed that the short-term HAP treatment stimulated the growth of cancer cells.     

Effect of siRNA Targeting Survivin Gene on the Biological Behavior of Hepatocellular Carcinoma

To study the influence of siRNA targeting survivin gene on the biological behavior of hepatocellular carcinoma (HCC), one pair of 21bp reverse repeated motifs of survivin target sequence with 9 spacers were synthesized and inserted into plasmid psilencer2.1 to generate siRNA eukaryotic expression vector. After stable transfection into HepG2 cells, the biological behaviors of the survivin siRNA transfected HCC cells were observed. After the recombinant plasmid Psilence( )-survivin was successfully constructed, survivin mRNA and protein expression inhibition ratio reached 73% and 75% respectively compared to control groups. Transfected cells with survivin siRNA demonstrated significantly inhibited cell growth and increased apoptosis. Subsequent study in nude mouse model demonstrated lower succeeding rate in cells transfected with survivin siRNA and slow growth rate. The results elucidated the siRNA targeting survivin gene could specially suppress its expression in HepG2 cells and inhibit tumor cells growth both in vivo and in vitro. This provides a theoretical basis to turn the drug resistance in tumor cells.     

The Biological Research of Clinical Behavior of Laryngeal Cancer

The present study analyzed the DNA content(ratio of heterploid,RH),s-phase cell(SPC)per-centage,the proliferating cell nuclear antigen(PC-NA) labelling index and area of nucleolar organizerregion (AgNOR) in 57 patients with laryngeal can-cer. The results were shown in Table 1.     

Effect of TheoPolyphenols(茶多酚)on Function of TransMembranous Signal System of Acute Ischemic Myocardium in Rats

ｔｉｓｔｈｅｍｙｏｃａｒｄｉａｌｉｓｃｈｅｍｉａａｎｄｉｓｃｈｅｍｉｃｒｅｐｅｒｆｕｓｉｏｎｉｎｊｕｒｙｔｈａｔｃｏｎｔｅｍｐｏｒａｒｙｓｔｕｄｙｆｏｃｕｓｓｅｄｕｐｏｎ．Ｉｔｈａｓｂｅｅｎｐｒｏｖｅｄｔｈａｔｔｈｅｏｐｏｌｙｐｈｅｎｏｌｓ（ＴＰ）ｈａｓｅｆｆｅｃｔｏｆａｎｔｉｏｘｙｇｅｎ,ｓｃａｖｅｎｇｉｎｇｏｘｙｇｅｎｆｒｅｅｒａｄｉｃａｌ,ａｄｊｕｓｔｉｎｇｓｅｒｕｍｌｉｐｉｄｓ,ｅｔｃ（１,２）．Ｂｕｔｉｔｉｓｕｎｃｌｅａｒｗｈａｔｉｓｉｔｓｍｏｌｅｃｕｌａｒｂｉｏｌｏｇｉｃａ…     

Effects of β-carotene on Biological Behavior of Human Bladder Cancer Cell Line T24

目的:探讨β-胡萝卜素对人膀胱癌T24细胞细胞增殖和凋亡的影响.方法:不同浓度的β-胡萝卜素作用于T24细胞,MTT法检测细胞增殖,流式细胞术检测T24细胞的凋亡指数.结果:MTT法检测结果显示10 μmol/L和20 μmol/L组β-胡萝卜素均能明显抑制T24细胞的生长,抑制率分别为28.83％和63.02％ (P＜0.05);流式细胞术检测显示β-胡萝卜素能诱导T24细胞调亡,5μmol/L组T24细胞的凋亡指数为(0.065±0.018) (P＜0.05),10 μmol/L和20 μmol/L组凋亡指数分别为(0.126±0.022)和(0.190±0.024)(P＜0.01),呈现剂量依赖效应,10 μmol/L组及20 μmol/L组Hochest染色可见凋亡小体形成.结论:β-胡萝卜素抑制T24细胞的生长,并且呈现剂量依赖效应,其机制可能为诱导细胞凋亡.     

Effect of DRB on the Biological Characteristics of Human Laryngeal Carcinoma Hep-2 Cell Line

In order to study the effect of 5, 6-Dichloro-1-β-D-ribofuranosyl-benzimidazole (DRB) on the biological characteristics of human laryngeal carcinoma Hep-2 cell line in vitro, Hep-2 cells cultured in vitro were treated with different concentrations of DRB. Changes in cell proliferation, apoptotic rate and invasiveness were detected by MTT assay, flow cytometry (FCM) and matrigel in vitro invasion assay, respectively. It was found that DRB inhibited the proliferation of Hep-2 cells in a dose- and time-dependent manner. After being treated with 0, 10, 20, 40, 80 μmmol/L DRB for 24 h, the apoptotic rate in Hep-2 cells was (0.68±0.19)%, (1.95±0.12)%, (8.51±0.26)%, (11.26±0.17)% and (14.99±0.32)%, respectively. The matrigel in vitro invasion assay revealed that DRB began to inhibit the invasion of Hep-2 cells at the concentration of 5 μmmol/L, and with the increase of DRB concen-tration, the inhibitory effect was enhanced. It was suggested that DRB could influence the essential biological characteristics of Hep-2 cells, inhibit Hep-2 cells proliferation, reduce invasive ability and induce apoptosis of Hep-2 cells.     

The Influence of Phorbol Ester on the Effect of Tamoxifen in Breast Cancer Cells

The growth of breastcells is stimulated simulta-neously by peptide growth factors and steroids,andithas been recently found thatthere are crosstalks inseveral levels between the two pathways. To eluci-date if the activation of extracelluar signal transduc-tion pathway influences the response of estrogen re-ceptor(ER) positivebreastcancer cellsto tamoxifen,we examined the influence of TPA,an activator ofprotein kinase C and mitogen activated protein ki-nase,on the effectof tamoxifen.1　 MATE…     

Study on Preventive and Therapeutic Effect of Radix Salviae Miltiorrhizae on Recurrence and Metastasis of Liver Cancer

Study on Preventive and Therapeutic Effect of Radix Salviae Miltiorrhizae on Recurrence and Metastasis of Liver Cancer@孙婧@周信达@刘银坤     

Effects of Exogenous p16^ink4a Gene on Biological Behaviors of Human Lung Cancer Cells

The effects of exogenous p16ink4a gene on biological behaviors of human lung cancer cell line with homozygous deletion of p16ink4a gene were investigated. Exogenous p16ink4a gene was transfected by lipofectin into human lung cell line A549, in which p16ink4a gene was homozygously deleted. The expression of p16ink4a mRNA and protein was detected by RT-PCR and immunocyto-chemistry, respectively. The changes in the behaviors of the transfected cell lines in vitro and in vivo were observed. In the transfected cell line A549, the exogenous p16ink4a gene could be stably ex-pressed. The growth of A549 cells transfected with p16ink4a gene was obviously slowed down. Flow cytometry revealed that transfection of the exogenous p16ink4a gene resulted in A549 cell lines arrest in G1 phase of cell cycle. The tumorigenicity of these transfected cells in nude mice could be inhib-ited, and the tumor growth of nude mice was significantly suppressed. It was concluded that exoge-nous p16ink4a gene may be stably expressed in human lung cancer cell line A549. The expression of the introduced p16ink4a could block lung cancer cells to entry into S phase of cell cycle and inhibit tumor malignant growth both in vitro and in vivo.     

Effect of Chinese Herbs Bu-shen on Expression of Pituitary PRL-R and PrRP-R in the Bromocriptine-induced Hypoprolactin Rat Abortion Model

Prolactin( PRL) playsan importantrole in early pregnancy,which has attractedmore and more attention in recent years.PRL plays a crucial role in facilitating thedevelopmentof ovary lutein cells and modulating the ovary function.Moreover,itistightly associated with the maintenanceof normal pregnancy as thehigh physiologicalPRL concentration is one of the characteristic features of early pregnancy.To investigate the role of PRL in maintaining pregnancy and clarifying the mechanisms of Bu-she…     

Experimental Study on Effect of Tonifying Kidney Herbs on Pituitary, Ovary, and Adrenal Gland in Androgen Sterilized Rats

Besidesthefunctionaldisturbanceofsexglandaxis,theabnormalfunctionofadrenalglandalsohasitseffectinthepathogenesisofhyperandrogenanovulatoryemmeniopathy.Inthisstudy,lightmicroscopy,electronmicroscopy,cellculture,argyrophilicnucleolarorganizerregion(AgNOR),andproliferativecellneucleolarantigen(PCNA)measurementwereusedtoobservesynchronouslythemorphologicandendocrinechangesofpituitarygland,ovaryandadrenalglandinandrogensterilizedrats(ASR)beforeandafteradministrationoftonifyingKidneyherbs(TKH),…     

Effect of Tiangui Recipe on Serum Leptin and Pituitary Gonadotropin in AndrogenSterilized Rats

Effect of Tiangui Recipe on Serum Leptin and Pituitary Gonadotropin in AndrogenSterilized Rats@孙斐@俞瑾     

The Chemopreventive Effect of Tamoxifen Combined with Celecoxib on DMBA chemically-Induced Breast Cancer

Objective: To investigate the chemopreventive effect of tamoxifen combined with a COX-2 selective inhibitor, celecoxib, on breast cancer in rats chemically induced by 7,12-dimethylben (a)anthracene (DMBA). Methods:DMBA was irrigated into the stomaches of SD female rats to build breast cancer model. A total of 120 rats were divided into four groups: control group, tamoxifen group, celecoxib group and combined group. The incidence rate, latent period, number and volume of breast cancer were detected and analyzed. Results:The tumor incidence rate of tamoxifen group (48.15%, 13/27) and celecoxib group (50.00%,14/28) were lower than that of control group (85.71%, 24/28), but higher than that of combined group (21.43%, 6/28). The tumor's latent period of tamoxifen group (97.54±1.85 d) and celecoxib group (96.79±2.89 d) were longer than that of control group (89.50±5.99 d), but shorter than that of combined group (103.67±3.39 d). The average tumor number of tamoxifen group (1.77±0.73) and celecoxib group (1.71±0.61) were less than that of control group (3.50±1.62), but more than that of combined group ( 1.17±0.42 ). The average tumor volume of tamoxifen group (1.78±0.71 cm3) and celecoxib group (2.05±1.04 cm3) were smaller than that of control group (6.42±3.96 cm3), but bigger than that of combined group (0.71±0.96 cm3) (P ＜ 0.05 respectively).Conclusion:Celecoxib and tamoxifen are effective drugs in preventing the occurrence of rat breast cancer chemically induced by DMBA. Furthermore, combination of them has better chemopreventive effect.     

Current Researches on Chinese Antioxidative Herbs

ＣｕｒｒｅｎｔＲｅｓｅａｒｃｈｅｓｏｎＣｈｉｎｅｓｅＡｎｔｉｏｘｉｄａｔｉｖｅＨｅｒｂｓＷＡＮＧＹｏｎｇ－ｊｕｎ（王拥军）；ＨＥＳｈｉ－ｄａ（何士大）（ＸｕａｎｗｕＨｏｓｐｉｔａｌ，ＣａｐｉｔａｌＵｎｉｖｅｒｓｉｔｙｏｆＭｅｄｉｃａｌＳｃｉｅｎｃｅｓ...