Weak electromagnetic fields (50 Hz) elicit a stress response in human cells

The aim of this study was to demonstrate the expression of heat shock (HS) genes in human cells in response to extremely low-frequency electromagnetic fields (ELF-EMF) alone and in combination with thermal stress. After exposing human myeloid leukemia (HL-60) cells to the stressor(s) for 30 min we quantified the expression of the HS genes HSP27, HSP60, HSP70 (A, B, and C), HSC70, HSP75, HSP78, and HSP90 (alpha and beta) by RT-PCR. The results clearly show that HS genes, in particular the three HSP70 genes (A, B, and C), are induced by ELF-EMF, a reaction that is enhanced by simultaneous HS (43 degrees C for 30 min). The results show similarities and some significant differences to previous experiments in which transgenic nematodes were used to monitor the induction of the HSP70 gene under similar stress conditions. We also studied the effect of different flux densities on gene expression in the range of 10-140 microT. Even the lowest dose tested (10 microT) resulted in a significant induction of the genes HSP70A, HSP70B, and HSP70C. The reaction to ELF-EMF shows a maximum at a flux density of 60-80 microT. The unusual dose-response relation reveals an interesting difference to other stressors that elicit the HS response.     

Genipa americana (Rubiaceae) fruit extract affects mitogen-activated protein kinase cell pathways in human trophoblast-derived BeWo cells: implications for placental development

Genipa americana L. (Rubiaceae) is a fruit tree and a traditional medicine used to treat anemia, icterus, asthma, and liver and spleen problems. The aim of the present study was to verify the effect of G. americana fruit ethanolic extract on the mechanism for proliferation and differentiation of trophoblast-like cells. Qualitative analysis of G. americana fruit extract was performed, and BeWo cells, a well-established placental choriocarcinoma cell line that can undergo differentiation, were used to analyze cell viability and proliferation. Methods consisted of cytotoxic and proliferation measurement, detection of release of human chorionic gonadotrophins, cell fusion observation, and evaluation of cell-signaling pathways (production of cyclic adenosine monophosphate and phosphorylation of mitogen-activated protein kinases [MAPKs]). A stock solution of the extract was diluted in Ham's F-12 medium with 10% fetal bovine serum at concentrations ranging from 50 to 1000?μg/mL. Cells treated with dimethylsulfoxide, forskoline, and MAPK inhibitors (PD98059 or SB203580) were used as a control. Forskoline was used to induce the differentiation state in BeWo cells. Phytoanalysis indicated the presence of steroids only. Results showed that the G. americana fruit extract did not cause any cytotoxicity or interference in cell differentiation. However, a significant antiproliferative state related to inhibition and reactivation of ERK1/2 and p38 MAPK in BeWo cells was seen. These results suggest that steroids from G. americana may affect placental cell regulation.     

RRR-alpha-tocopheryl succinate modulation of human promyelocytic leukemia (HL-60) cell proliferation and differentiation.

HL-60 human promyelocytic leukemia cells can be induced to differentiate to granulocytes by retinoic acid and dimethyl sulfoxide or monocyte-macrophages by phorbol esters and 1,25-dihydroxyvitamin D3. These studies show that RRR-alpha-tocopheryl succinate (TS) inhibits HL-60 cell proliferation and induces the HL-60 cells to differentiate toward a functionally deficient macrophage-like cell. TS at (15 micrograms/ml) was found to suppress HL-60 cell proliferation by 63% and 89% at 24 and 48 hours, respectively. This suppression of proliferation, however, is not permanent and requires the presence of TS. HL-60 cells treated for 48 hours with TS (15 micrograms/ml) were found to be blocked in the G2/M phase of the cell cycle. HL-60 cells blocked in the G2/M cell cycle phase by TS expressed normal levels of the transferrin receptor. TS-treated HL-60 cells exhibited binucleated morphological appearance; however, the cells did not exhibit chemotaxis, phagocytosis, or changes in the expression of the cell surface markers, CD11a and CD18. However, HL-60 cells treated for 48 hours with TS (15 micrograms/ml) could be stimulated to produce superoxide radicals and exhibited nonspecific esterase activity, two characteristics of macrophages. These results suggest a role for TS as an antitumor proliferative agent and as a modifier of human leukemia cell differentiation.     

辛伐他汀抑制鼻咽癌细胞增殖和亲环素A表达

目的研究辛伐他汀对鼻咽癌细胞株CNE2细胞增殖和细胞周期的作用以及对亲环素A表达的影响。方法用CCK-8方法观察辛伐他汀对鼻咽癌细胞株CNE2细胞增殖的抑制作用,用流式细胞仪检测辛伐他汀对鼻咽癌细胞株CNE2细胞周期分布的影响,用蛋白免疫印迹检测亲环素A蛋白表达。结果CCK－8方法结果显示,辛伐他汀抑制鼻咽癌细胞株CNE2细胞增殖,作用72h时Ic50为2．0μmol／L。流式细胞仪结果表明辛伐他汀将鼻咽癌细胞株CNE2细胞阻滞在细胞周期的G0／G1期。蛋白免疫印迹结果显示辛伐他汀明显抑制亲环素A蛋白表达,以上效应均呈浓度依赖性。结论辛伐他汀能浓度依赖性地抑制CNE2细胞增殖．将细胞阻滞在G0／G1期。同时伴随有亲环素A表达减少。     

辛伐他汀抑制鼻咽癌细胞增殖和亲环素A表达

目的研究辛伐他汀对鼻咽癌细胞株CNE2细胞增殖和细胞周期的作用以及对亲环素A表达的影响。方法用CCK-8方法观察辛伐他汀对鼻咽癌细胞株CNE2细胞增殖的抑制作用,用流式细胞仪检测辛伐他汀对鼻咽癌细胞株CNE2细胞周期分布的影响,用蛋白免疫印迹检测亲环素A蛋白表达。结果CCK-8方法结果显示,辛伐他汀抑制鼻咽癌细胞株CNE2细胞增殖,作用72h时IC50为2.0μmol/L。流式细胞仪结果表明辛伐他汀将鼻咽癌细胞株CNE2细胞阻滞在细胞周期的G0/G1期,蛋白免疫印迹结果显示辛伐他汀明显抑制亲环素A蛋白表达,以上效应均呈浓度依赖性。结论辛伐他汀能浓度依赖性地抑制CNE2细胞增殖,将细胞阻滞在G0/G1期,同时伴随有亲环素A表达减少。     

Ganglioside composition of differentiated Caco-2 cells resembles human colostrum and neonatal rat intestine

Gangliosides are glycosphingolipids found in cell membranes and human milk with important roles in cell proliferation, differentiation, growth, adhesion, migration, signalling and apoptosis. Similar changes in ganglioside composition occur during embryonic development, lactation and cancer cell differentiation. It is not known, however, whether ganglioside compositional changes that occur in differentiating colon cancer cells reflect changes that occur during intestinal development. The Caco-2 cell line is commonly used to study physiological and pathophysiological processes in the small intestine and colon. Therefore, to examine this question, undifferentiated and differentiated Caco-2 cells were grown and total lipid was extracted from cell supernatant fractions using the Folch method. The upper aqueous phase containing gangliosides was collected and purified. Total gangliosides were measured as ganglioside-bound N-acetyl neuraminic acid, while individual ganglioside content was quantified via a colorimetric assay for sialic acid and scanning densitometry. The total ganglioside content of differentiated Caco-2 cells was 2.5 times higher compared with undifferentiated cells. Differentiated Caco-2 cells had significantly more (N-acetylneuraminyl) 2-galactosylglucosyl ceramide (GD3) and polar gangliosides, and a lower N-acetylneuraminylgalactosylglucosylceramide (GM3):GD3 ratio than undifferentiated cells. The present study demonstrates that the total ganglioside content and individual ganglioside composition of differentiated Caco-2 cells are similar to those of human colostrum and neonatal rat intestine. Differentiated Caco-2 cells may therefore be an alternative model for studying physiological and pathological processes in the small intestine and colon, and may help to elucidate possible functions for specific gangliosides in development and differentiation. Further research using more sensitive techniques of ganglioside analysis is needed to confirm these findings.     

Conjugated linoleic acid inhibits differentiation of pre- and post- confluent 3T3-L1 preadipocytes but inhibits cell proliferation only in preconfluent cells

Conjugated linoleic acid (CLA; 18:2) is a group of isomers (mainly 9-cis, 11-trans and 10-trans, 12-cis) of linoleic acid. CLA is the product of rumen fermentation and can be found in the milk and muscle of ruminants. Animals fed CLA have a lower body fat content. The objective of this study was to establish the possible mechanisms by which CLA affects adipogenesis. 3T3-L1 is a well-established cell line that is used extensively in studying adipocyte biology. These cells typically grow in a culture medium until they reach confluence, at which time they are induced to differentiate by hormonal treatment (d 0). Treatment of 3T3-L1 cells with 25 to 100 micromol/L CLA inhibited differentiation in a dose-dependent manner, while linoleic acid treatment did not differ from DMSO-treated controls. Continuous treatment from d -2, -1, 0 or 2 to d 8 and treatment from d -2 to d 0 and from d 0 to d 2 inhibited differentiation. Differentiation was monitored morphologically (oil Red-O staining), enzymatically (reduction of activity of glycerol-3-phosphate dehydrogenase), and by northern analysis of peroxisome proliferator-activated receptor gamma2, CCAAT/enhancer binding protein alpha and adipocyte specific protein 2 mRNA. CLA inhibited cell proliferation of nonconfluent cells but did not affect cell division of confluent cells, as indicated by 5-bromo-2'-deoxyuridine incorporation and mitochondria metabolism. Therefore, CLA inhibited differentiation before confluence and during induction. However, cellular proliferation was only inhibited prior to induction. These results imply that fat reduction caused by CLA treatment may be attributed to its inhibition of both proliferation and differentiation of preadipocytes in animals.     

极低频脉冲电磁场对不同来源成骨细胞的影响

目的 研究极低频脉冲电磁场对成骨样细胞增殖和分化的影响。方法 MC3T3-E1细胞和取自2日龄SD大鼠颅骨的原代成骨细胞分别暴露于48Hz、1．55mT的磁场环境下24和48h。利用2-对磺酸基苯基-3-（4，5-二甲基噻唑）-5-（3-羧甲氧基苯基）-二氢四唑嗡盐法（MTS）和流式细胞仪对细胞的增殖和细胞周期的变化进行检测；用比色法对胞内碱性磷酸酶（ALP）活力进行检测。结果 磁场暴露24和48h后。MC3T3-E1细胞的S期或G2M期比例明显降低，与对照组比较，差异有统计学意义（P〈0．05）。但对其细胞数量无明显影响。原代成骨细胞连续磁场暴露24h后，细胞数无明显变化。但G2M期比例明显升高。与对照组的差异有统计学意义（P〈0．01）。原代细胞以不同的暴露方式处理48h。其细胞数均明显增加，而G：M期比例则明显降低，与对照组比较，差异均有统计学意义（P〈0．01）。原代成骨细胞暴露于脉冲磁场24h后，ALP活力明显降低；而当原代成骨细胞连续48h暴露于脉冲磁场后。ALP活力则明显增高。与对照组比较，差异均有统计学意义（P〈0．05）。结论 48Hz、1．55mT的脉冲磁场暴露对成骨样细胞系MC3T3-E1细胞增殖和分化均无明显影响。对于原代成骨细胞，48Hz、1．55mT的脉冲磁场暴露可促进其细胞增殖，在细胞增殖初期具有抑制分化的作用，而在细胞分化期则具有但排锌化的作用     

Ginsenoside Rb1 promotes PC12 cell cycle kinetics through an adenylate cyclase–dependent protein kinase A pathway

Ginsenoside Rb1 (G-Rb1), a constituent of ginseng, bears various beneficial effects on neuroendocrine cells. Previous studies have indicated that G-Rb1 can enhance glutamate release in undifferentiated and differentiated PC12 cells via the protein kinase A (PKA)–dependent signaling pathway. We hypothesized that G-Rb1 stimulates rat adrenomedullary chromaffin cell line PC12 (PC12 cells) proliferation and mitosis by promoting the cell cycle at all regulatory points. This mechanism is partly mediated via the adenylate cyclase–dependent PKA signaling pathway. In the present study, we investigated the mechanism by which G-Rb1 promotes cell cycle kinetics from the PC12 cells. The cell cycle kinetics of these cells were determined using flow cytometric DNA analysis. Analysis of the PC12 cell cycle revealed that G-Rb1 may affect all phases of the cell cycle and accelerate cell cycle kinetics by stimulating G0G1 phase transiting to S and G2M phases. The cell cycle kinetics were decreased by coincubating with the adenylate cyclase inhibitor SQ22536. Compared with the G-Rb1–treated group, the PKA inhibitor H89 produced a marked decrease in the G-Rb1–stimulated cell cycle kinetics by inhibiting G0G1 phase from transiting to the S phase. These results support the position that G-Rb1 exerts a stimulatory effect on cell cycle kinetics to promote PC12 cell proliferation. The result also suggests that the division rate is mediated via the adenylate cyclase–dependent PKA signaling pathway.     

基因Ercc6l在P19胚胎癌细胞内的表达研究

[目的]探讨基因Ercc6l(excision repair cross-complementing rodent repair deficiency,complementation group6-like)在P19胚胎癌细胞分化过程中的表达情况。[方法]用二甲亚砜(DMSO)和视黄酸(RA)分别诱导P19细胞向心肌细胞方向和神经细胞方向分化,RT-PCR检测P19细胞诱导分化过程中Ercc6l mRNA的表达变化。[结果]RT-PCR检测表明,基因Ercc6l在未分化的P19细胞中不表达,但当P19细胞诱导分化为心肌细胞或神经细胞后,Ercc6l表达显著上调。[结论]Ercc6l在调控胚胎细胞分化和胚胎发育过程中具有重要作用。     

移动电话射频电磁场诱导膜受体聚簇及噪声磁场的干预

目的 研究GSM 1 800 MHz射频电磁场（以下简称“射频场”）对细胞膜表皮生长因子（EGF）受体聚簇的可能诱导作用及噪声磁场的干预.方法 将中国仓鼠肺成纤维细胞（CHL）分别用1 800 MHz射频场（包括217和50 Hz调制和非调制）、噪声磁场和射频场与噪声磁场叠加的复合场处理15 min,用EGF处理作为阳性对照,射频场的比吸收率（SAR）值取0.1、0.5、1.0、2.0和4.0W/kg.上述处理后的细胞经间接免疫荧光染色标记后,用激光共聚焦显微镜观察其细胞膜EGF受体的聚簇现象.结果 SAR值为0.5、1.0、2.0和4.0W/kg的调制射频场辐照CHL细胞15 min可诱导细胞膜EGF受体的聚簇,但当SAR值为0.1 W/kg时,细胞膜不出现EGF受体聚簇.而非调制射频场以及2μT噪声磁场不能诱导细胞膜EGF受体的聚簇,当2μT噪声磁场与射频场叠加后,可抑制射频场诱导的细胞膜EGF受体聚簇.结论 GSM1 800 MHz射频场能诱导细胞膜EGF受体的聚簇,波的调制在其中起主要作用;一定强度的噪声磁场可阻断GSM 1 800 MHz射频场诱导的细胞膜EGF受体聚簇.     

罗格列酮与全反式维甲酸对人胃癌SGC7901细胞株生长和凋亡的影响

目的 研究PPARγ激动剂罗格列酮（RSG）与全反式维甲酸（ATRA）对人胃癌SGC7901细胞株生长和凋亡的影响及其机制的研究.方法 体外培养SGC7901细胞,实验分为空白对照组、10μmol/L ATRA组、12.5 μmol/L RSG、25 μmol/L RSG组,10 μmol/L ATRA和25 μmol/L RSG联合组.MTT法检测细胞生长抑制率,流式细胞仪测定细胞周期,HE染色检测细胞形态,免疫细胞化学检测PPARγ蛋白,RT-PCR检测PPARγmRNA.结果 10 μmol/L ATRA、12.5 mmol/L RSG、25 mmol/L RSG及两药联合时皆可抑制SGC7901细胞的增殖,且存在浓度及时间依赖,两药联合作用72 h时,生长抑制率为（29.73±0.69）%.ATRA、RSG皆可使细胞周期G0/G1期延长,S期下降,两药联合作用时,S%为（12.87±0.35）%,细胞形态向正常方向转化,细胞的PPARγ蛋白核内表达及PPARγmRNA表达上调,两药联合后作用进一步加强,PPARγ/GAPDH的浓度比值高达0.646.结论 ATRA、RSG均可抑制SGC7901细胞增殖,阻滞细胞周期,诱导细胞分化和上调PPARγ蛋白及PPARγmRNA的核内表达,ATRA和RSG联合较单药作用效果更强.     

稳恒磁场对大鼠胚胎中脑神经细胞发育的影响

目的　研究稳恒磁场对中脑神经细胞分化和增殖的磁场强度效应关系 ,并探讨其机制。方法　利用大鼠胚胎中脑神经细胞进行原代微团培养 ,观察不同磁场强度 1 0、10 0、5 0 0、10 0 0、2 0 0 0mT的稳恒磁场对细胞分化和增殖的影响 ,并利用图像分析细胞形态变化、硫代巴比妥酸测定丙二醛和流式细胞仪测定蛋白总量。结果　磁场强度 (≥ 10 0mT)会抑制胚胎中脑细胞分化和增殖 ,集落形成率明显减少、细胞体积小 ,并显示磁场强度效应关系 ,拟合细胞分化和增殖抑制曲线 ,计算得中脑神经细胞半数分化抑制磁场强度 (ICD50 )为 2 5mT ,半数存活抑制磁场强度 (IVD50 )为 4 5mT。结论　稳恒磁场能抑制中脑神经细胞分化和增殖可能与其蛋白质和脂质过氧化物合成有关     

Embryonale Stammzellen der Maus Eigenschaften, Potenzial und Verwendung

During the last few years research on embryonic stem cells has received much public attention due to the fact that these cells are able to differentiate in vitro into many specialized cells and thus may serve as a source for a variety of tissues. The following article focuses on mouse embryonic stem cells (murine ES cells), because research on these cells has given insight into the potential of embryonic stem cells. Murine ES cells are permanent cell lines established from the inner cell mass (ICM) of early embryos (blastocysts). ES cells are undifferentiated pluripotent cells that are able to undergo an unlimited number of cell divisions without loosing the undifferentiated phenotype. The same is true for mouse primordial germ cell lines (murine EG cell lines), that where established from the fetal progenitor cells of primordial germ cells. Mouse embryonic stem cells are used for different purposes. In basic research they are used to study the consequences of mutations within genes that control embryonic development and/or the development of diseases. Because of their ability to differentiate into a variety of specialized cell types, murine ES cells also serve as model systems to establish specific differentiation protocols. In the last few years protocols were established for the in vitro development of undifferentiated embryonic stem cells into differentiated cardiac, skeletal muscle, neural, adipogenic, haematopoietic, endothelial, chondrogenic or vascular smooth muscle cells. Last but not least, studies on mouse ES cells have demonstrated that embryonic cells and their differentiated derivatives can be used to analyse the effects of toxic substances or of pharmaceutical drugs.     

Risks of leukaemia among residents close to high voltage transmission electric lines.

OBJECTIVES: To reassess the risk of leukaemia associated with residential exposure near high voltage transmission electric lines of 49 kV and above in view of the recent publications. METHODS: Through a review of papers considering the risk of leukaemia among people living near high voltage transmission lines, the combined risks of leukaemia were calculated for distances < or = 50 m and < or = 25 m and for exposures at 2 mG, 3 mG, 4 mG, and 10 mG. RESULTS: The combined analysis of the contributive studies indicated an estimate of risk (odds ratio (OR)) for exposure > or = 2 mG of 1.3, 95% confidence interval (95%CI) 1.0 to 1.7. The ORs increased with exposures at 3 mG, 4 mG, and 10 mG. The risks were also increased for distances of 50 and 25 m from the lines. CONCLUSIONS: This meta-analysis tends to confirm the presence of an association between exposure to magnetic fields and leukaemia among people who reside in the vicinity of high voltage transmission electric lines of > or = 49 kV. There is consistency across studies. Measures of exposure used in the studies were either distance from the lines or calculated fields estimated from pertinent line features. The results apply to adults as well as to children.     

Lycopene and 1,25-dihydroxyvitamin D3 cooperate in the inhibition of cell cycle progression and induction of differentiation in HL-60 leukemic cells

Lycopene, the major tomato carotenoid, has been found to inhibit proliferation of several types of cancer cells, including those of breast, lung, and endometrium. By extending the work to the HL-60 promyelocytic leukemia cell line, we aimed to evaluate some mechanistic aspects of this effect. Particularly, the possibility was examined that the antiproliferative action of the carotenoid is associated with induction of cell differentiation. Lycopene treatment resulted in a concentration-dependent reduction in HL-60 cell growth as measured by [3H]thymidine incorporation and cell counting. This effect was accompanied by inhibition of cell cycle progression in the G0/G1 phase as measured by flow cytometry. Lycopene alone induced cell differentiation as measured by phorbol ester-dependent reduction of nitro blue tetrazolium and expression of the cell surface antigen CD14. Results of several recent intervention studies with beta-carotene, which have revealed no beneficial effects of this carotenoid, suggest that a single dietary component cannot explain the anticancer effect of diets rich in vegetables and fruits. Thus another goal of our study was to examine whether lycopene has the ability to synergize with other natural anticancer compounds, such as 1,25-dihydroxyvitamin D3, which when used alone are therapeutically active only at high and toxic concentrations. The combination of low concentrations of lycopene with 1,25-dihydroxyvitamin D3 exhibited a synergistic effect on cell proliferation and differentiation and an additive effect on cell cycle progression. Such synergistic antiproliferative and differentiating effects of lycopene and other compounds found in the diet and in plasma may suggest the inclusion of the carotenoid in the diet as a cancer-preventive measure.     

Human diploid MRC-5 cells exhibit several critical properties of human umbilical cord-derived mesenchymal stem cells

MRC-5 is the most common human diploid cell line used in production of viral vaccines; mesenchymal stem cells (MSCs) is a type of adult multipotent stem cells. Both cell types share the same fibroblast-like morphology and maintain a normal diploid karyotype over long in vitro expansion. However, other than these similarities, very little is known about MRC-5 in terms of biological properties possessed by MSCs. In this study, we compared MRC-5 with human umbilical cord-derived MSCs (hUC-MSCs), which serves as a representative of human MSCs, in expression of cell surface markers, abilities to differentiate into multiple cell lineages, inhibition of lymphocyte proliferation and promotion of Regulatory T lymphocytes (Treg), and IDO1 expression in response to inflammatory cytokines, all of which are critical properties of MSCs. It was revealed that MRC-5 was almost identical to hUC-MSCs in expression of both positive and negative surface markers of MSCs. Similar to hUC-MSCs, MRC-5 was also able to differentiate into osteocytes and chondrocytes, effectively inhibit mitogen-activated lymphocyte proliferation and promote Tregs, and express IDO1 in response to inflammatory cytokines IFN-γ and TNF-α. In addition, both MRC-5 and hUC-MSCs were non-tumorigenic with an extremely low telomerase activity. Moreover, both cells demonstrated a similar sensitivity to infection by EV71 and rubella viruses, which served as model viruses, in a virus infectivity assay. Therefore, this study suggests that MRC-5 is very likely a previously undefined MSC cell line, thus suggesting the feasibility of developing MSCs of at least umbilical cord origin as new cell substrates to be used in production of viral vaccines.     

维生素E同系化合物抑制人肝癌细胞HepG2增殖的作用

通过探讨α 生育酚 (α T)、γ 生育酚 (γ T)、δ 生育酚 (δ T)及维生素E琥珀酸酯 (VES)抑制肝癌细胞生长的生物学效应 ,为维生素E(VE)防治肝癌提供理论依据 ,在体外培养的人肝癌细胞 (HepG2 )培养液中分别加入 1 2 5、2 5 0、50 0、1 0 0 0和 2 0 0mg L的VE同系化合物 ,采用细胞计数法和噻唑蓝比色法 (MTT)比较VE同系化合物抑制HepG2细胞增殖的效应 ;采用流式细胞仪检测四种VE同系化合物对HepG2细胞周期的影响。结果显示在 1 2 5和 2 5 0mg L浓度下 ,4种VE同系化合物均未显示出对HepG2细胞生长的抑制效应 ;在50、1 0 0和 2 0 0mg L浓度下 ,δ 生育酚、VES处理的HepG2生长速度明显减缓、细胞存活率显著降低 ,δ 生育酚处理的HepG2细胞生长轻度减缓 ,而γ 生育酚处理的HepG2细胞未见明显改变。VE同系化合物抑制HepG2细胞增殖的效应为δ 生育酚 >VES >γ 生育酚 >α 生育酚 ;细胞周期结果显示HepG2细胞增殖阻断于G1 期。提示VE同系化合物抑制肝癌细胞生长作用不同 ,δ 生育酚和VES在体外显示出较强的抗肝癌细胞增殖效应     

天然红心蛋中类胡萝卜素提纯物对人肝癌细胞增殖和凋亡的影响

目的:探讨天然红心蛋中类胡萝卜素提纯物(carotenoidsextractsfromnaturalredyolk,CENRY)对人肝癌细胞QGY-7703细胞增殖和凋亡的影响。方法:采用体外培养,细胞增殖实验,激光扫描共聚焦显微镜(LSCM)观察细胞形态及钙离子浓度变化,采用流式细胞术检测细胞周期等。结果:CENRY1.0、0.5、0.25μmol/L三个剂量能显著抑制人肝癌QGY-7703细胞增殖,细胞凋亡指数随剂量增加及时间延长而上升;CENRY对QGY-7703细胞存在G2/M期阻滞作用;处理组细胞内Ca2+浓度极显著地升高。结论:CENRY能抑制QGY-7703增殖、诱导凋亡。