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1.
S. C. Halos E. S. Fortuno III A. C. M. Ferreon J. Y Chu J. Miranda S. Harada M. Benecke 《International journal of legal medicine》1998,111(4):224-226
Allele frequency distributions at the short tandem repeat (STR) loci HUMVWA, HUMFES, HUMF13A01 and of the variable number
of tandem repeat (VNTR) locus D1S80 were determined in a Filipino population from Metro Manila (103 individuals) by use of
the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE). The exact test demonstrated that
all four loci had no deviations from Hardy-Weinberg equilibrium (HWE) with the only reservation that the exact test p-value
for F13A01 is weak. The discriminating power is 0.82 for D1S80, and the expected exclusion chance is 0.85 for F13A01, 0.83
for FES, and 0.93 for VWA. The observed heterozygosity rates are 0.63 for D1S80, 0.66 for F13A01, 0.67 for FES, and 0.80 for
VWA. The exact test for independance between all loci gave a p-value of 0.0195. This is the first time that Filipino population
data of DNA loci of forensic importance are reported.
Received: 7 July 1997 / Received in revised form: 18 September 1997 相似文献
2.
Dong Hoon Lee Jae Seok Han Woo Ghil Lee Seong Whan Lee Hyune Mo Rho 《International journal of legal medicine》1998,111(6):320-322
Multiplex PCR amplification has been useful for gene mapping with polymorphic short tandem repeat (STR) loci. We have tested
the four loci D20S470, D13S325, HumFOLP23 and D10S2325 for the simultaneous typing of more than 100 unrelated Koreans. This
analysis allows a single base pair resolution and rapid typing with silver staining. The allele and genotype distributions
are in accordance with Hardy – Weinberg expectations. These STR loci have proven useful for forensic analysis and paternity
tests in which the variable number of tandem repeat (VNTR) loci have some limitations.
Received: 25 November 1997 / Received in revised form: 25 February 1998 相似文献
3.
M. Hantschel R. Hausmann T. Lederer P. Martus P. Betz 《International journal of legal medicine》1999,112(6):393-395
Frequency data for nine short tandem repeat (STR) loci were collected from 130 unrelated Caucasians from North Bavaria using
the AmpFlSTR Profiler multiplex system. The loci D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317, D7S820 and the sex
test amelogenin were investigated. Allele frequencies, rates of heterozygosity and the discrimination power of the combined
systems were calculated by statistical analysis. Except for D5S818 all loci met Hardy-Weinberg expectations.
Received: 27 August 1998 / Received in revised form: 28 December 1998 相似文献
4.
Steinlechner M Schmidt K Kraft HG Utermann G Parson W 《International journal of legal medicine》2002,116(3):176-178
Allele frequencies for ten short tandem repeat (STR) loci D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433,
TH01 and FGA were determined in a Black African sample population from Gabon. All loci were highly polymorphic and except
for TH01, D21S11 and D16S539, all met Hardy-Weinberg expectations. There was little evidence of association of alleles between
the loci in this database. The combined power of exclusion for the ten STR loci was 0.999981. While significant differences
between the Gabon population and the Austrian Caucasian population were found at all loci, significant differences were found
between the Gabon population and Zimbabweans only for D3S1358 and between the Gabon population and African Americans only
for TH01 and D8S1179.
Received: 14 March 2001 / Accepted: 15 May 2001 相似文献
5.
P. Martín O. García C. Albarrán P. García M. Sancho A. Alonso 《International journal of legal medicine》1999,112(5):340-341
Population data were generated for four tetrameric short tandem repeat loci systems (D8S1179, D16S539, D18S51 and D21S11)
for a Spanish Caucasian population sample (n = 218–219 individuals) using PCR. All loci were highly polymorphic, met Hardy-Weinberg
expectations and the results demonstrated the assumption of independence of the loci analysed. The allele frequency data can
be used in identity testing to estimate the frequency of a multiple PCR-based DNA profile in the Spanish population.
Received: 5 October 1998 / Received in revised form: 8 December 1998 相似文献
6.
Steinlechner M Berger B Scheithauer R Parson W 《International journal of legal medicine》2001,114(4-5):288-290
A population study on the ten short tandem repeat (STR) loci D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433,
TH01 and FGA was performed on 204 unrelated Austrian Caucasians. The DNA was amplified by multiplex PCR using the AmpFℓSTR
SGM plus kit. All loci met Hardy-Weinberg expectations. The combined power of exclusion for the ten STR loci was 0.999976.
The results show that these loci are very useful for forensic purposes.
Received: 28 February 2000 / Accepted: 22 May 2000 相似文献
7.
Blood samples from 409–452 unrelated Colombian Caucasian-Mestizo individuals were amplified and typed for six short tandem
repeat (STR) markers (HUMF13A01, HUMFES/FPS, HUMVWA, HUMCSF1PO, HUMTPOX, HUMTH01). The allele frequencies, genotype frequencies,
heterozygocity, mean paternity exclusion chance, polymorphism information content, discrimination power, assumption of independence
within and between loci and Hardy Weinberg equilibrium were determined. The results demonstrate that all markers conform to
Hardy-Weinberg equilibrium expectations. In addition, the results demonstrate the assumption of independence within and between
the loci analysed. The mean exclusion chance (MEC) was 0.9851 for all six STR loci analysed and the discrimination power (DP)
was 0.9999973. Therefore, this Colombian population database can be used in identity testing to estimate the frequency of
a multiple PCR-based locus DNA profile in forensic cases as well as in paternity testing.
Received: 24 September 1998 / Received in revised form: 22 December 1998 / Accepted: 11 January 1999 相似文献
8.
Xiadong X Xunling W Xiaolin H Guifang R Xiaolin L 《International journal of legal medicine》2001,114(6):349-351
Frequency data for nine tetrameric short tandem repeat loci (D16S539, D7S820, D13S317, HUMF13A01, FESFPS, vWA, HUMTH01, TPOX
and CSF1PO) were investigated in a population sample of 107 unrelated Tibetan individuals by using a multiplex polymerase
chain reaction (PCR), followed by 4% polyacrylamide gel electrophoresis (PAGE) and silver staining. All loci met the Hardy-Weinberg
expectations. The forensically relevant parameters were calculated. This is the first time that Chinese Tibetan population
data on DNA loci have been reported that are of forensic importance.
Received: 8 February 2000 / Accepted: 7 November 2000 相似文献
9.
Allele frequencies of 13 short tandem repeats in population samples from the Iberian Peninsula and Northern Africa 总被引:3,自引:0,他引:3
Pérez-Lezaun A Calafell F Clarimón J Bosch E Mateu E Gusmão L Amorim A Benchemsi N Bertranpetit J 《International journal of legal medicine》2000,113(4):208-214
The 13 short tandem repeat (STR) loci D3S1358, vWA, FGA, D16S539, TH01, TPOX, CSF1PO, D8S1179, D21S11, D18S51, D5S818, D13S317
and D7S820 as well as the amelogenin locus, contained in AmpFlSTR Profiler Plus and/or AmpFlSTR Cofiler and/or AmpFlSTR Green I PCR amplification kits, were studied in four populations from the Iberian Peninsula, Basques, Catalans, Andalusians
and Portuguese and two North African populations (Moroccan Arabs and Berbers). The aim of the study was to obtain accurate
allele frequency data and other genetic parameters of forensic interest on the main representative human groups living in
Iberia and Morocco using an automated method and commercial amplification kits.
Received: 15 March 1999 / Received in revised form: 25 June 1999 相似文献
10.
Allele and genotype frequencies for six short tandem repeat (STR) loci were determined in a sample of 124 inhabitants from
South Poland with commercial PCR-based typing kits. No deviations from Hardy-Weinberg expectations were found. The combined
power of discrimination for the six loci was 0.9999982. There was no genotypic disequilibrium between the loci except for
vWA and F13A01. The set of PCR loci was validated as useful for paternity testing and individual identification in the Polish
population.
Received: 2 November 1998 / Received in revised form: 5 March 1999 相似文献
11.
A Korean population study of the nine STR loci FGA, VWA, D3S1358, D18S51, D21S11, D8S1179, D7S820, D13S317 and D5S818 总被引:1,自引:0,他引:1
Han GR Lee YW Lee HL Kim SM Ku TW Kang IH Lee HS Hwang JJ 《International journal of legal medicine》2000,114(1-2):41-44
DNA typing was performed on 379 randomly selected unrelated Koreans using the nine short tandem repeat loci FGA, VWA, D3S1358,
D18S51, D21S11, D8S1179, D7S820, D13S317 and D5S818 present in the AmpFlSTR Profiler Plus PCR amplification kit. Allele frequencies, heterozygosity, power of discrimination, mean exclusion chance,
and polymorphism information content of each locus were calculated by statistical analysis. All nine loci were in Hardy-Weinberg
equilibrium. The combined discrimination index and the combined mean exclusion chance in Koreans was 2.31 × 10–12 and 0.99983, respectively. By evaluation of 297 children from 128 families, 2 mutations were found at the FGA locus and 1
each at the D18S51 and D13S317 loci. This study demonstrates that this multiplex system is a useful and convenient tool for
forensic identification and parentage testing in Korea.
Received: 15 September 1999 / Accepted: 14 January 2000 相似文献
12.
New reference allelic ladders to improve allelic designation in a multiplex STR system 总被引:4,自引:0,他引:4
R. A. L. Griffiths M. D. Barber P. E. Johnson S. M. Gillbard M. D. Haywood C. D. Smith J. Arnold T. Burke A. J. Urquhart P. Gill 《International journal of legal medicine》1998,111(5):267-272
This paper reports the composition of a new reference allelic ladder mixture for use with a multiplex DNA profiling system
consisting of six short tandem repeat loci. The loci included in this mixture are HUMTH01, D21S11, D18S51, D8S1179, HUMVWAF31/A,
HUMFIBRA/FGA and an amelogenin sex test. Sequence analysis of individual ladder alleles was carried out and allelic designations
made in accordance with the recommendations of the International Society of Forensic Haemogenetics (1992; 1994). A series
of rare alleles which increase the range of alleles previously reported were identified. By including some of the rare alleles
into the ladder marker system, we have significantly improved the ability to identify new alleles in unknown samples.
Received: 12 August 1997 / Received in revised form: 7 November 1997 相似文献
13.
Asamura H Uchida R Takayanagi K Ota M Fukushima H 《International journal of legal medicine》2006,120(3):182-184
Allele frequencies and forensic parameters for the six miniSTR loci D1S1677, D2S441, D4S2364, D10S1248, D14S1434, and D22S1045 were investigated in a sample of 142 unrelated healthy Japanese individuals. The polymerase chain reaction (PCR) products contained within the six loci were less than 119 bp in size. The frequency distributions in the six short tandem repeat (STR) loci showed no deviations from Hardy–Weinberg equilibrium expectations. The accumulated powers of discrimination and power of exclusion for the six loci were 0.999998 and 0.98, respectively. It was thus considered that due to the small PCR products and the moderate degree of polymorphism, analysis with use of the six miniSTR loci was highly beneficial for the forensic analysis of degraded DNA. 相似文献
14.
Population data for 12 STR loci in Hong Kong Chinese 总被引:3,自引:0,他引:3
Wong DM Law MY Fung WK Chan KL Li C Lun TS Lai KM Cheung KY Chiu CT 《International journal of legal medicine》2001,114(4-5):281-284
The allele distributions at the 12 short tandem repeat (STR) loci D3S1358, HUMvWA, HUMFIBRA/FGA, HUMTHO1, HUMTPOX, HUMCSF1P0,
D5S818, D13S317, D7S820, D8S1179, D21S11 and D18S51 have been determined for 284 unrelated Chinese in Hong Kong. The combined
probability of identity for the 12 STR loci was about 4.1 × 10–14 and the overall probability of excluding paternity 0.999978. None of the 12 loci were found to deviate from Hardy-Weinberg
expectations according to the results of the exact test. There was also little evidence for association of alleles between
loci. The results demonstrate that the loci are useful for forensic human identification and parentage testing for the Chinese
population in Hong Kong.
Received: 2 December 1999 / Accepted: 12 April 2000 相似文献
15.
Germline mutations of 24 short tandem repeat (STR) loci (TPOX, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, TH01, vWA, D13S317,
Penta E, D16S539, D18S51, Penta D, D21S11, D2S1772, D6S1043, D7S3048, D8S1132, D11S2368, D12S391, D13S325, D18S1364, and GATA198B05)
were studied for 6,441 parent–child meioses taken from the paternity testing cases in Chinese Han population. In total, 195
mutations were identified at 22 of the 24 loci. Among them, 189 (96.92%) mutations were one step, five mutations (2.56%) were
two step, and one mutation (0.51%) was three step. No mutation was found at the TH01 and TPOX loci. The overall mutation rate
estimated was 0.0013 (95% CI 0.0011–0.0015), and the locus-specific mutation rate estimated ranged from 0 to 0.0034. There
was a bias in the STR mutations that repeat gains were more common than losses (∼1.7:1). Mutation events in the male germline
were more frequent than in the female germline (∼4.3:1). Furthermore, loci with a larger heterozygosity tended to have a higher
mutation rate. Mutation in short alleles was biased towards expansion, whereas mutation in long alleles favored contraction.
The long alleles have a higher allelic mutational probability than short alleles. 相似文献
16.
Albeza MV Picornell A Acreche N Tomas C Castro JA Ramon MM 《International journal of legal medicine》2002,116(3):126-132
Frequency data of the short tandem repeat (STR) loci D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820,
HUMTH01, D4S243, D18S535, HUMF13A1 and D12S391 were determined in the Puna population (Andean region from north western Argentina).
In this study, 5 out of the 14 loci did not meet Hardy-Weinberg expectations. The excess of homozygotes observed in most of
the markers could be due to a Wahlund effect and/or inbreeding. The frequencies were significantly different from those of
other compared populations from Europe and America. Forensic parameters indicated that the 14 loci studied in the Puna region
are highly discriminating, but the lack of Hardy-Weinberg equilibrium in some of the markers must be taken into account in
the application of these results to paternity and forensic casework.
Received: 3 January 2001 / Accepted: 20 September 2001 相似文献
17.
In a Kurdish population sample composed of 950 unrelated individuals from Northern Iraq, 11 tetrameric short tandem repeat (STR) loci from 10 different chromosomes (i.e., ACTBP2, CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D13S317 and D21S11) were typed to establish a database for immigration cases. The combined power of discrimination (PD) and the combined power of exclusion (PE) of all 11 loci were 0.99999999999994 and 0.99996, respectively. 相似文献
18.
M. Gené E. Piqué N. Borrego A. Carracedo E. Huguet P. Moreno 《International journal of legal medicine》1998,112(1):75-77
Allele frequencies for four short tandem repeat loci were determined in a population sample from Catalonia (NE Spain). After
denaturing PAGE electrophoresis, 8 alleles were identified for D3S1358 (n = 201), 10 alleles for D8S1179 (n = 198), 13 alleles for D18S51 (n = 197) and 11 alleles for D19S253 (n = 201). No deviation from Hardy-Weinberg equilibrium was found. Complete and relative uniformity in Caucasoid populations
has been observed for D18S51 and D8S1179 respectively. Pronounced differences were found between different ethnic groups for
both systems. Catalonia and Portugal do not differ for D3S1358 locus. Multiplex PCR amplifications of three loci (D3S1358,
D18S51 and D19S253) without overlapping fragment size ranges could be interesting for monochrome automated laser fluorescence
devices.
Received: 15 January 1998 / Received in revised form: 20 April 1998 相似文献
19.
We examined six human short tandem repeat (STR) loci (c-fms proto-oncogene for CSF-1 receptor, CSF1PO; thyroid peroxidase, TPOX; tyrosine hydroxylase, TH01; coagulation factor XIII a subunit, F13A01; c-fes/fps proto-oncogene, FESFPS and von Willebrand factor, vWA) in the Japanese macaque, Macaca fuscata, using commercially available human STR kits. No products were amplified by polymerase chain reaction (PCR) using the human CSF1PO, TH01, FESFPS and vWA primers. Macaque DNAs were amplified with human TPOX and F13A01 primers. Macaque PCR products amplified with the TPOX and F13A01 primers migrated more slowly than human ones during electrophoresis. Sequencing results showed that the nucleotide sequences of the counterpart of the TPOX and F13A01 STR loci in the Japanese macaque were closely similar to those of the human genes except for tandem repeat regions. The macaque products amplified with human TPOX and F13A01 primers were highly polymorphic, with four variants of the former and 15 variants of the latter in the nine samples. These results indicate that the commercially available kits can be used to discriminate the Japanese macaque samples from human samples. 相似文献
20.
Population genetic studies were carried out on randomly selected and unrelated healthy individuals from Sicily (n = 140–150 individuals) using the short tandem repeat (STR) systems HumTH01, HumvWA31, HumFESFPS and HumF13A01. After vertical
electrophoresis on polyacrylamide denaturing gels 6 alleles could be identified for TH01, 9 for vWA31, 7 for FESFPS and 11
for F13A01. No significant deviations from Hardy-Weinberg were observed.
Received: 9 June 1997 / Received in revised form: 14 January 1998 相似文献