封闭负压引流技术对创面血管化的影响

目的 观察封闭负压引流技术对创面血管化的影响.方法 制作幼猪肌腱外露创面模型2×12个,设为封闭负压引流组(VSD组)和对照组,对表达CD31的新生血管、表达α-平滑肌肌动蛋白(α-SMA)的成熟血管和表达血管内皮生长因子(VEGF)的细胞数进行统计学比较.结果 第7天,VSD组CD31阳性新生血管数为(19.90±1.36)个,α-SMA阳性成熟血管数为(11.90±1.32)个,VEGF阳性新生血管数为(69.90±1.27)个,CD31 mRNA表达量为(1.543±0.072);对照组分别为(13.20±1.02)、(8.20±1.05)、(44.20 ±0.72)个以及(0.988 ±0.031),差异有统计学意义(P＜0.05).结论 封闭负压引流能加速创面血管化,肉芽覆盖肌腱,促进创面愈合.     

负压封闭引流治疗高位肛周脓肿的临床研究

探讨负压封闭引流治疗高位肛周脓肿的疗效。94例高位肛周脓肿患者按数字随机表法分为两组,每组47例。治疗组给予负压封闭引流治疗,对照组给予低位切开引流治疗,比较两组手术时间、住院时间、创面愈合时间、住院费用;比较两组术后第2日疼痛视觉模拟评分(VAS)和Wexner肛门失禁评分;比较两组术前及术后7 d血清肿瘤坏死因子-α(TNF-α)和表皮生长因子(EGF)水平;术后4周比较两组治愈率。治疗组术后住院时间、创面愈合时间及住院费用均少于对照组(P<0.01);术后第2日治疗组VAS评分、Wexner评分均低于对照组(P<0.01);术后7 d治疗组血清TNF-α水平低于对照组(P<0.01),EGF水平高于对照组(P<0.01),术后4周治疗组治愈率高于对照组(P<0.05),差异均有统计学意义。负压封闭引流术治疗高位肛周脓肿可有效保护肛门功能,缓解术后疼痛,减少创面愈合时间和住院时间,提高治愈率。     

负压封闭引流联合解毒洗药灌注治疗糖尿病足溃疡1例

糖尿病足溃疡(diabetic foot ulcer,DFU )是由神经病变、外周动脉闭塞及创伤伴继发感染联合引起的一种糖尿病常见并发症,是糖尿病患者致残、致死的主要原因之一,临床患病率逐年上升[1].Meta分析发现DFU的全球平均患病率为6.3％,男性的患病率高于女性,2型糖尿病的患病率高于1型糖尿病[2].负压封...     

负压封闭引流技术治疗糖尿病足创面的护理体会

总结我院2010年10月－2011年10月使用负压封闭引流技术（VSD）治疗糖尿病足溃疡患者46例的护理体会。 1临床资料46例糖尿病足溃疡患者中男29例,女17例;年龄53～81岁,平均（67．0±17．3）岁。创面5～20cm2,均为Wagner3级,其中累及皮肤及浅筋膜层36例,肌腱外露13例,骨外露7例。足底17例,足背13例,足外侧缘9例,足内侧缘7例。所有创面经手术彻底清创后,行VSD（VSD材料由武汉维斯第医用有限公司生产）,方法参考文献[1]。调整负压并维持一125～一300mmHg。根据创面渗出情况,术后用庆大霉素液（160kU加入到500ml生理盐水中）持续冲洗。所有患者经治疗均部分或完全达到肉芽组织覆盖。     

封闭负压引流对软组织爆炸创面的影响

[目的]观察封闭负压引流(vacuum sealing drainage,VSD)对软组织爆炸创面愈合的影响,为战场中所致的爆炸伤初级救治提供新的思路.[方法]用电雷管在8只(50±5)kg的长白猪双侧臀部造成16个爆炸伤创面,配对分为实验组和对照组.实验组创面彻底清创后,VSD治疗3d,然后打开伤口对创面进行简单的清洗,再用VSD治疗3d.对照组创面彻底清创后,每E3常规换药.[结果]两组创面细菌数在治疗后3、6d均有显着性差异(P<0.01),实验组细菌数始终没有达到感染临界值105,而对照组3d后的细菌数已达到106;治疗3、6d后实验组新生肉芽组织中的胶原含量和对照组比较存在显著性差异(P<0.01),实验组高于对照组;并且两组创面中血管内皮生长因子(VEGF)的含量在治疗3、6d后也出现显著性差异(P<0.01),实验组高于对照组.[结论]爆炸创面早期运用VSD疗法,可抑制细菌增殖,促进肉芽组织形成和伤口愈合.     

改良负压封闭引流联合创面胰岛素滴注技术治疗糖尿病足的疗效评估与分析

目的评估与分析改良负压封闭引流联合创面胰岛素滴注技术治疗糖尿病足的临床疗效。方法纳入自2016-01—2019-12治疗的50例糖尿病足(2型糖尿病且足部溃疡至少2周),观察组28例清创后采用改良负压封闭引流联合胰岛素创面局部滴注治疗,对照组22例清创后采用改良负压封闭引流联合生理盐水创面局部滴注治疗。结果观察组肉芽组织覆盖率高于对照组,肉芽组织厚度大于对照组,细菌清除率高于对照组,差异有统计学意义(P0.05)。观察组治疗后TNF-α、IL-1β、IL-6、VCAM-1表达明显低于对照组,而VEGF、TGF、EGF表达高于对照组,差异有统计学意义(P0.05)。观察组采用皮瓣覆盖创面的比例低于对照组,且植皮成活率与皮瓣成活率高于对照组,差异有统计学意义(P0.05)。结论糖尿病足创面采用改良负压封闭引流联合胰岛素滴注灌洗技术治疗可获得满意的临床疗效,该方法既保留了负压封闭引流技术治疗创面的优点,又融入了持续灌洗技术的优点,同时加入了胰岛素起到降糖、抗炎促进创面愈合的作用,降低了并发症发生的风险。     

封闭负压引流术促进创面愈合的机制探讨

创面愈合是临床治疗的难点,特别是创伤面较大的体表皮肤置换手术,以及创口大、污染重的重大手术患者,创面愈合的好坏与患者术后恢复效果、并发症发生率、伤口愈合后的美观度有直接的联系。封闭负压引流术在创面愈合中有积极的效果,得到了临床广泛的应用和研究。     

负压封闭引流技术联合重组人表皮生长因子治疗糖尿病足溃疡效果分析

目的 探讨负压封闭引流技术(VSD)联合重组人表皮生长因子(rhEGF)治疗糖尿病足溃疡的临床效果。方法 回顾性分析2019-01—2021-02中国人民解放军联勤保障部队第九八八医院整形烧伤科收治的50例糖尿病足溃疡患者的临床资料,分为对照组和联合组,各25例。2组均给予常规清创处理及VSD。联合组联合rhEGF注射。比较2组患者的溃疡创面愈合时间、住院时间、住院费用及不良反应。评价2组的临床治疗效果。结果 联合组溃疡创面愈合时间和住院时间均短于对照组,住院费用少于对照组,治疗总有效率高于对照组,差异均有统计学意义(P<0.05)。治疗期间2组患者均未出现严重不良反应。结论 VSD联合rhEGF治疗糖尿病足溃疡,溃疡创面愈合快、患者住院时间短、住院费用低、治疗总有效率高,而且无不良反应。是安全、可行性的治疗方案。     

封闭负压引流技术对人慢性创面中透明质酸含量的影响

目的研究人慢性创面在经封闭负压引流治疗前后,创面渗出液中透明质酸含量的变化,部分阐明封闭负压引流促进慢性创面愈合的机理。方法取8例乳癌术后第1、2、3、4天急性创面的引流液,同时收集11例慢性创面(5例压力性溃疡,6例静脉性溃疡)于封闭负压引流治疗前以及治疗后第1、3、5、7、9天的渗出液,利用放射免疫测定的方法分别测定其中透明质酸的含量,并进行动态的观察与比较。结果急性创面引流液中随时间延长透明质酸含量逐渐增高,各时间点的差异有显著意义(P<0.05)。压力性溃疡中,封闭负压引流治疗前渗出液中透明质酸含量较低,引流后随治疗时间的延长透明质酸水平升高(第1、3、5、7天差异有显著意义P<0.05),至第9天有下降趋势;而静脉性溃疡中,封闭负压引流治疗前透明质酸水平极高,治疗后透明质酸含量随时间延长呈现极明显的波动,但于第7天后呈下降趋势。结论封闭负压引流通过平衡透明质酸的代谢从而促进慢性创面的愈合,这可能是封闭负压引流促进慢性创面愈合的一个重要机制。     

封闭负压引流技术对人慢性创面中透明质酸含量的影响

目的研究人慢性创面在经封闭负压引流治疗前后,创面渗出液中透明质酸含量的变化,部分阐明封闭负压引流促进慢性创面愈合的机理.方法取8例乳癌术后第1、2、3、4天急性创面的引流液,同时收集11例慢性创面(5例压力性溃疡,6例静脉性溃疡)于封闭负压引流治疗前以及治疗后第1、3、5、7、9天的渗出液,利用放射免疫测定的方法分别测定其中透明质酸的含量,并进行动态的观察与比较.结果急性创面引流液中随时间延长透明质酸含量逐渐增高,各时间点的差异有显著意义(P＜0.05).压力性溃疡中,封闭负压引流治疗前渗出液中透明质酸含量较低,引流后随治疗时间的延长透明质酸水平升高(第1、3、5、7天差异有显著意义P＜0.05),至第9天有下降趋势;而静脉性溃疡中,封闭负压引流治疗前透明质酸水平极高,治疗后透明质酸含量随时间延长呈现极明显的波动,但于第7天后呈下降趋势.结论封闭负压引流通过平衡透明质酸的代谢从而促进慢性创面的愈合,这可能是封闭负压引流促进慢性创面愈合的一个重要机制.     

改良负压封闭引流技术治疗糖尿病足的疗效观察

目的:观察应用改良负压封闭引流(VSD)技术治疗糖尿病足的效果.方法:将糖尿病足40例患者随机分为对照组和治疗组.对照组应用传统VSD技术治疗,治疗组应用改良VSD技术治疗,即在VSD敷料中增加一根冲洗管,经冲洗管每日1次注入呋喃西林500 ml和表皮生长因子30 ml,30 min后关闭冲洗管,开启负压.于治疗10 ...     

银离子敷料结合负压引流技术在糖尿病足溃疡中的应用及疗效观察

目的 分析银离子敷料结合负压引流技术在糖尿病足溃疡中应用的安全性及疗效。方法 选择2014年12月～2016年12月期间于我院进行的患者共110例为研究对象,按照随机化原则将其分为观察组（55例）和对照组（55例）,对照组采用常规清创术+凡士林纱布,观察组采用银离子敷料+负压引流技术,比较两组患者创面愈合情况、换药次数、住院时间、术后VAS疼痛评分及治疗效果。结果 观察组新鲜肉芽组织出现时间、创面愈合时间、换药次数及住院时间明显少于对照组［（6.74±1.86vs10.55±1.73）d、（33.04±5.82vs42.29±4.77）d、（8.87±2.85vs20.29±3.06）次、（15.38±5.51vs22.08±4.85）d］,而创面缩小百分比明显高于对照组［（48.83±7.37vs34.49±6.94）%］,差异有统计学意义（P<0.05）。观察组、对照组患者治疗后VAS疼痛评分明显低于治疗前［（2.71±1.39vs5.44±1.58）分、（4.13±1.44vs5.51±1.49）分］,差异有统计学意义（P<0.05）;治疗后观察组的VAS疼痛评分明显低于对照组［（2.71±1.39vs4.13±1.44）分］,差异有统计学意义（P<0.05）。观察组治疗有效率明显高于对照组（90.91%vs66.45%）,差异有统计学意义（P<0.05）。结论 银离子敷料结合负压引流技术可有效治愈糖尿病足溃疡,促进患者的康复。     

Safety,efficacy and pitfalls of fibrocyte application in the treatment of diabetic foot ulcer

Fibrocytes are unique bone marrow‐derived cells with great potential in wound healing. Hence, the aim of this study was to determine the safety and efficacy of the applied circulating fibrocytes in the treatment of non healing diabetic foot ulcers. Peripheral blood mononuclear cells were isolated by centrifugation through Ficoll–Paque method. After 3 days, the non adherent cells were removed by a single, gentle aspiration. Adherent cells were cultured in the same medium for 10 days. The cells were characterised using mouse anti‐human‐CD45‐fluorescein isothiocyanate (FITC) and mouse anti‐human–collagen I, and also characterised by immunofluorescence microscopy using the above mentioned antibodies. Sterility measures were applied for clinical evaluation. Based on the literature review, cell transplantation generally requires at least 3 × 10⁶ cells regarding efficacy measures. As fibrocytes are non proliferating cells, 350 ml patient's blood is required to prepare patient‐specific serum before cell isolation and culture, and 85 ml patient's blood is needed for cell isolation and differentiation on cell transplantation applications. In our survey, no diabetic patient was inclined to be donor of such blood volume, mainly because of their pre‐assumption that they are anaemic. It is concluded that fibrocytes do not seem to be candidate cells for cell therapy in the treatment of diabetic foot ulcers because of the rarity of this cell population in circulation.     

负压封闭引流及湿性愈合技术在小儿尿道下裂治疗中的效果评价

目的探讨应用负压封闭引流技术(VSD)及湿性愈合技术在小儿尿道下裂治疗中的有效性及安全性。方法2015年1月至2017年1月郑州大学第一附属医院小儿泌尿外科收治尿道下裂Ⅲ度伴中重度阴茎下弯的患儿80例,年龄1岁6个月至12岁,按随机数字表法随机分为观察组和对照组,每组40例。80例患儿由同一位术者行阴茎下弯矫正术+一期尿道成形术(横裁包皮岛状皮瓣卷管术,Duckett术式),所有患儿术中均游离翻转阴囊中隔筋膜组织瓣覆盖加固于成形尿道。术毕观察组阴茎包裹软聚硅酮伤口接触层敷料(美皮贴)后安装VSD,术后生理盐水持续缓慢冲洗并低负压持续加压引流7 d,负压维持在-30^-20 kPa,液体滴速为每分钟7滴;对照组常规应用美皮贴、纱布及自粘型弹力绷带加压包扎7 d。2组打开VSD及包扎敷料后均常规碘伏消毒,于阴茎切口血痂处交替应用红霉素药膏涂抹及生理盐水纱布湿敷软化血痂使其尽快脱落,2组术后均常规留置F8硅胶尿管1个月。观察比较2组术后切口处血痂发生、血痂下感染、尿道瘘、尿道裂开、尿道狭窄等情况。使用SPSS 21.0统计软件进行数据处理,计数资料比较采用t检验,计量资料比较采用χ2检验,P<0.05为差异有统计学意义。手术成功评价标准为无尿道瘘、尿道狭窄、尿道裂开、尿道憩室等术后并发症,排尿通畅,无排尿困难。结果观察组患儿对VSD的耐受性良好,无VSD不良反应。术后7 d,阴茎敞开暴露后,观察组阴茎切口表面血痂发生率为20.0%(8/40),血痂下感染发生率为12.5%(5/40),均低于对照组的42.5%(17/40)和32.5%(13/40),差异均有统计学意义(χ2=4.713,P=0.030;χ2=4.588,P=0.032)。术后1个月拔除尿管后观察组发生尿道瘘5例,尿道狭窄1例,手术成功率85.0%(34/40);对照组发生尿道瘘9例,尿道裂开1例,尿道狭窄1例,手术成功率72.5%(29/40),观察组手术成功率高于对照组。结论小儿尿道下裂术后应用VSD持续生理盐水冲洗并低负压加压引流治疗效果满意并安全,能有效减少尿道下裂术后血痂的形成及血痂下感染的发生,有助于提高尿道下裂修复手术的成功率。     

Application of vacuum sealing drainage to the treatment of seawater‐immersed blast‐injury wounds

The aims of this study were to observe the effects of vacuum sealing drainage (VSD) with three different negative pressures on the wound healing rate, macrophage count and the expression of hyaluronic acid (HA) as well as its receptor CD44 in seawater‐immersed blast‐injury wounds (SIBIW) and to determine the optimal negative pressure value. In a minipig SIBIW model, different suction pressures and routine dressing were applied. Histological and immunohistochemical comparisons as well as molecular biology methods were performed to compare the wound healing conditions, macrophage count and the levels of HA and CD44. The wound healing rate of the VSD group was significantly higher than that of the control group, with the ?120 mmHg group exhibiting the best effects. The macrophage count of the VSD group was higher than that of the control group. The HA level fluctuation was higher in the VSD group, with the ?120 mmHg and the ?180 mmHg groups showing the most significant fluctuation (P < 0·05). CD44 was expressed in the full‐thickness wound‐limbic tissues and was higher in the treatment group than that in the control group, with the ?120 mmHg group having the most obvious expression. VSD significantly improved the healing ability and increased the macrophage count and the HA content. It also promoted CD44 expression. ?120 mmHg is the optimal negative pressure value.     

封闭式负压引流技术在重度皮肤潜行性剥脱伤中的应用

目的探讨封闭式负压引流技术(vacuum sealing drainage,VSD)联合中厚皮片回植治疗重度皮肤潜行性剥脱伤(closed internal degloving injury,CIDI)的疗效。方法 2008年7月-2011年4月,收治16例重度皮肤CIDI患者。男11例,女5例;年龄17～56岁,平均28岁。均为交通事故伤。受伤至手术时间2～8 h,平均5 h。皮肤剥脱部位:上肢3例,下肢13例。皮肤剥脱范围为5%～12%体表面积,平均7%体表面积。剥脱皮肤均有不同程度污染。彻底清创后,切取剥脱皮肤制备为断层中厚皮片原位回植,术后应用VSD治疗。结果 14例经7 d VSD治疗后,回植皮肤顺利成活;2例VSD治疗7 d后约0.3%体表面积回植皮肤发生坏死,再次彻底清创后VSD联合抗生素治疗,7 d后游离植皮修复后愈合。患者均获随访,随访时间6～18个月,平均12个月。肢体外观满意,无明显瘢痕形成,血运及感觉正常;关节均活动良好。结论重度皮肤CIDI患者剥脱皮肤回植后应用VSD治疗,可改善肢体局部血液循环,促进回植皮肤成活,缩短回植皮肤成活时间,临床疗效满意。     

应用VSD治疗跟骨骨折术后伤口不愈合

目的:探讨清创VSD封闭引流技术治疗跟骨骨折术后伤口不愈合的效果。方法:2008年6月至2011年12月,收治跟骨骨折术后伤口缺血坏死、伤口不愈合的患者11例,其中男10例,女1例;年龄21～55岁,平均39岁;病程7～9周。利用手术清创的同时联合负压封闭引流伤区灌注技术治疗,术后参考张铁良跟骨关节内骨折疗效标准评估疗效。结果:伤区经VSD封闭引流后自体植皮均顺利愈合。11例患者术后获得随访,时间6～18个月,平均11个月,伤口无迟发感染及慢性骨髓炎发生。依据张铁良跟骨关节内骨折疗效评价标准:优6例,良4例,可1例。结论:跟骨骨折术后伤口愈合不良联合VSD封闭引流治疗能获得满意的疗效。     

Adiponectin as a therapeutic target for diabetic foot ulcer

The global burden of diabetic foot ulcers (DFUs) is a significant public health concern, affecting millions of people worldwide. These wounds cause considerable suffering and have a high economic cost. Therefore, there is a need for effective strategies to prevent and treat DFUs. One promising therapeutic approach is the use of adiponectin, a hormone primarily produced and secreted by adipose tissue. Adiponectin has demonstrated anti-inflammatory and anti-atherogenic properties, and researchers have suggested its potential therapeutic applications in the treatment of DFUs. Studies have indicated that adiponectin can inhibit the production of pro-inflammatory cytokines, increase the production of vascular endothelial growth factor, a key mediator of angiogenesis, and inhibit the activation of the intrinsic apoptotic pathway. Additionally, adiponectin has been found to possess antioxidant properties and impact glucose metabolism, the immune system, extracellular matrix remodeling, and nerve function. The objective of this review is to summarize the current state of research on the potential role of adiponectin in the treatment of DFUs and to identify areas where further research is needed in order to fully understand the effects of adiponectin on DFUs and to establish its safety and efficacy as a treatment for DFUs in the clinical setting. This will provide a deeper understanding of the underlying mechanisms of DFUs that can aid in the development of new and more effective treatment strategies.     

Intralesional administration of epidermal growth factor‐based formulation (Heberprot‐P) in chronic diabetic foot ulcer: treatment up to complete wound closure

Previous studies have shown that an epidermal growth factor‐based formulation (Heberprot‐P) can enhance granulation of high‐grade diabetic foot ulcers (DFU). The aim of this study was to explore the clinical effects of this administration up to complete wound closure. A pilot study in 20 diabetic patients with full‐thickness lower extremity ulcers of more than 4 weeks of evolution was performed. Mean ulcer size was 16·3 ± 21·3 cm². Intralesional injections of 75 μg of Heberprot‐P three times per week were given up to complete wound healing. Full granulation response was achieved in all 20 patients in 23·6 ± 3·8 days. Complete wound closure was obtained in 17 (85%) cases in 44·3 ± 8·9 days. Amputation was not necessary in any case and only one relapse was notified. The most frequent adverse events were tremors, chills, pain and ardour at site of administration and local infection. The therapeutic scheme of intralesional Heberprot‐P administration up to complete closure can be safe and suitable to improve the therapeutic goal in terms of healing of chronic DFU.     

自体皮肤移植术联合负压封闭引流技术治疗难治性慢性下肢静脉溃疡的临床疗效

目的 探讨自体皮肤移植术联合负压封闭引流技术(VSD)治疗难治性慢性下肢静脉溃疡(VLU)的临床疗效.方法 收集2012年3月至2020年9月上海市第六人民医院收治的114例难治性慢性VLU患者的临床资料,根据治疗方式的不同将患者分为观察组(n=53)和对照组(n=61),观察组在常规静脉曲张手术的基础上对VLU创面采...