Human papillomavirus DNA in the dermis of condyloma acuminatum

Condyloma acuminatum (CA) has high recurrence rates after local treatments. Why this lesion is difficult to eradicate is unclear. One possible explanation for recurrence after superficial destructive therapy is the presence of residual human papillomavirus (HPV) in the superficial dermis beneath the treated epidermis. Thirteen samples of CA were excised from 13 patients. Thirteen samples of basal cell carcinoma (BCC) were studied for purposes of control. Epidermis was separated from dermis by treatment with sodium bromide. DNA was extracted from both tissues and used sodium bromide solution and amplified for the presence of HPV DNA using the polymerase chain reaction. HPV DNA was detected in the epidermis of 11 samples of CA. HPV type 6 was seen in 7 specimens; HPV type 11, in 4. HPV DNA was found in the dermis of 3 specimens of CA; type 6 in 2 and type 11 in 1. Two samples were excluded because of contamination of the sodium bromide solution by HPV. HPV DNA was not detected in tissue samples from BCC. The presence of HPV DNA in the dermis of some condylomata may explain recurrence in sporadic cases.     

幼儿肛周扁平湿疣误诊为尖锐湿疣1例

患儿,女,28个月。肛周扁平丘疹2月,无自觉症状,实验室检查:梅毒螺旋体凝集试验(TPPA)阳性,快速血浆反应素试验(RPR)1∶128,祖父祖母TPPA均为阳性,RPR均为1∶8,父母梅毒血清学检查结果均阴性,诊断为获得性二期梅毒(肛周扁平湿疣)。予苄星青霉素60万u/周治疗,3周后皮损基本消退,一月后患儿RPR降至1∶8。     

男男性接触者肛周及肛管内尖锐湿疣患者HPV分型与复发的关系

目的 探讨上海男男性接触者肛周及肛管内尖锐湿疣患者人类乳头瘤病毒（HPV）感染基因分型情况及不同基因型别与临床复发的关系。方法 导流杂交基因芯片技术对57例MSM尖锐湿疣患者肛周及肛管内皮损进行HPV基因分型研究,并随访高危型组,低危型组经CO2激光治疗后尖锐湿疣的复发率。结果 57例MSM尖锐湿疣患者肛周及肛管内皮损检测出56例HPV阳性,占98.2%,共检出11种HPV基因亚型,以HPV11（42例,占75%）和HPV6（30例,占53.6%）为主,检出病例数从高到低依次为HPV11 > HPV6 > HPV16 > HPV18 > HPV33、59、cp8304 > HPV31、35。56例HPV阳性患者中低危型感染36例,占64.3%,均为HPV11和（或）HPV6;高危型感染20例,占35.7%,其中17例为低危型与高危型混合感染,占高危型感染的85%。56例患者经CO2激光治疗后随访6个月,高危型组复发率为95%,低危型组复发率为66.7%,两者差异有统计学意义（P < 0.05）。结论 上海男男性接触者尖锐湿疣HPV基因型主要以低危型HPV11、6为主,高危型感染大多合并低危型感染,高危型组复发率明显高于低危型组。     

Human papillomavirus and extragenital in situ carcinoma

BACKGROUND: The relation between human papillomavirus (HPV) and extragenital Bowen's disease (BD) is controversial. METHODS: This study used in situ hybridisation to evaluate the rate of HPV in extragenital cutaneous BD and investigated possible relations with immune status and exposure of skin to light. RESULTS: HPV DNA was detected in 58% of 69 samples from 50 patients. The percentage of HPV detection was not significantly higher in exposed (55%) than unexposed areas (65%), and no difference in HPV rate was found between immunosuppressed and immunocompetent patients. CONCLUSION: Thus, this study confirms the high rate of HPV detection in extragenital cutaneous BD and suggests that there is no apparent relation concerning exposed areas and immune status.     

203例女性外阴尖锐湿疣患者宫颈HPV基因型检测及分析

目的:探讨206例女性外阴尖锐湿疣(CA)患者宫颈感染人乳头瘤病毒( HPV)的基因型分布.方法:采用核酸分子快速导流杂交基因芯片技术,对203例女性外阴尖锐湿疣患者宫颈进行HPV感染分型检测.结果:在203例宫颈标本中,本芯片共能检测21种HPV亚型,本实验检测出20种,未发现HPV43型.203份宫颈组织标本中HPV阳性123例,检出率是60.59％,其中单一型别阳性率是65.04％ (80/123),多重型别阳性率是34.95％ (43/123);各型别检出率由高到低依次是HPV11 (35.77％)、6(28.45％)、16( 12.19％)、52( 12.19％)、58(8.98％)、68(8.13％)、66( 6.50％)、CP8304 (5.69％),18(4.87％)、33(4.87％)、44(4.87％)、39(3.25％).结论:根据本组病例,对于女性外阴尖锐湿疣患者,应重视宫颈HPV基因分型的检测;高危型及多重型别感染者有必要进一步检查及随访.     

CO_2激光治疗男男性接触者肛周及肛管内尖锐湿疣疗效分析

目的:探讨CO_2激光治疗男男性接触者肛周及肛管内尖锐湿疣的疗效。方法:对140例男男性接触者肛周及肛管内尖锐湿疣进行5点法麻醉下CO_2激光治疗,并进行末次治疗后6个月的随访。结果:单纯肛周组83例,第1次治疗后有31例(37.3%)复发,至末次治疗结束后,随访6个月均治愈(100%);单纯肛管组21例,第1次治疗后有13例(61.9%)复发,6个月后有1例复发,治愈率95.2%;肛周+肛管组36例,第1次治疗后有24例(66.7%)复发,6个月后有3例复发,治愈率91.7%。创面愈合期为2~6周,所有患者均有暂时性疼痛、肛门狭窄、排便困难,一般在2~3个月中解除,功能完全恢复。结论:CO_2激光治疗男男性接触者肛周及肛管内尖锐湿疣疗效可靠,不良反应少。     

尖锐湿疣皮损中高危型和低危型人乳头瘤病毒感染情况研究

目的:研究尖锐湿疣皮损中高危型和低危型人乳头瘤病毒(Human papillomavirus,HPV)感染情况。方法:研究纳入235例人乳头瘤病毒检查结果阳性的患者,对其型别、各型感染率及病毒拷贝数进行统计分析。结果:235例人乳头瘤病毒感染阳性患者,存在348人次人乳头瘤病毒感染,统计结果如下:HPV6和HPV11(36.7%,128/348)、HPV16(7.5%,26/348)、HPVl8(3.5%,12/348)、HPV31(1.7%,6/348)、HPV33(2.9%,10/348)、HPV35(2%,7/348)、HPV39(5.2%,18/348)、HPV45(1.1%,4/348)、HPV51(5.7%,20/348)、HPV52(11.5%,40/348)、HPV56(2.9%,10/348)、HPV58(10.1%,35/348)、HPV59(5.5%,,19/348)、HPV68(3.7%,13/348)。235例患者中128例为尖锐湿疣低危型HPV6及HPV11感染,高危型HPV中HPV52感染率最高,其次为HPV58,然后依次为HPV16,HPV51,HPV59,HPV39,HPV68,HPV18,HPV33,HPV56,HPV33,HPV31,感染率最低的是HPV45。感染低危型HPV6及HPV11患者为128例,只感染低危型HPV6及HPV11的患者为82例;感染高危型HPV患者为153例,只感染高危型HPV的患者为107例。低危型和高危型混合感染患者为46例。HPV感染拷贝数主要集中在107-103/104细胞。结论:尖锐湿疣中HPV6和11阳性率最高,高危型HPV可以和低位型HPV合并感染,也可以单独感染,发病率呈增高趋势,因此需要加强尖锐湿疣患者的治疗至关重要。     

女性外阴尖锐湿疣患者伴发宫颈高危型HPV感染的研究

目的: 确定女性外阴尖锐湿疣患者宫颈高危型HPV的感染.方法: 采用美国FDA批准的第二代杂交捕获试验对112例女性外阴尖锐湿疣患者的宫颈脱落细胞进行高危型HPV DNA检测.结果:112例患者中,宫颈高危型 HPV DNA阳性率为66.07％(74/112),其中子宫颈薄层液基细胞学检测(LCT)正常者42例,轻度宫颈上皮细胞病变者32例(非典型鳞状细胞20例,低度上皮内病变者12例),伴有宫颈上皮细胞病变与否在高危型HPV DNA平均载量方面无显著性差异(P＞0.05).结论: 女性尖锐湿疣患者常合并宫颈高危型HPV感染,有必要进行宫颈高危型HPV常规检测.     

应用间接法原位PCR技术检测尖锐湿疣组织中HPV感染

应用间接原位ＰＣＲ技术，对３２例经ＨＰＶ６ｂ、１１型探针原位杂交检测呈阴性结果的尖锐湿疣组织进行了再次检测。在光镜下诊断为尖锐湿疣的１０例中，有４例为阳性，阳性率为４０％，符合尖锐湿疣的２２例中，有８例为阳性，阳性率为３６．４％，总的再次检出率为３７．５％（１２／３２）。结果提示：间接法原位ＰＣＲ能够克服原位杂交方法的假阴性，明显提高检出率，并具有结果可靠，特异性强的特点，有重要研究和应用价值     

Giant condyloma acuminatum in a baby boy.

A giant condyloma acuminatum developed on the penis of a one and a half-year-old Japanese boy in two months. The histological features of this tumor were compatible with those of ordinary condyloma acuminatum. Although we detected the presence of human papilloma virus (HPV) type 16 by using the polymerase chain reaction system, we could not rule out the possibility that this HPV was present concurrently with other HPV types that cause condyloma acuminatum. The lesion was successfully treated with cryotherapy and topical application of 5% fluorouracil ointment under occlusion.     

Characteristic intracytoplasmic material in a perianal wart that contains human papillomavirus type 56 and 16 DNA

We report the case of a perianal wart in which human papillomavirus type 56 and 16 DNAs were detected, arising in a 29-year-old healthy Japanese female. Clinically, moist erythema and small flat pinkish papules appeared around the anus and worsened over a period of 6 months. Histopathologically, no signs of dysplasia or atypia were apparent. An area of intracytoplasmic material, which was homogeneous and eosinophilic, was contained in each epidermal cell. The wart showed HPV-56 and HPV-16 DNAs by PCR and reverse-phase dot blot hybridization. Additional Southern blot analysis showed the amount of HPV-56 DNA to be more than 10 times that of HPV-16 DNA. This is the first case, to our knowledge, of a perianal wart with intracytoplasmic material, which contains HPV DNAs causing mucous lesions.     

原位分子杂交检测尖锐湿疣组织中HPV DNA的研究

本文采用HPV DNA原位分子杂交法检测了30例尖锐湿疣组织标本，结果显示HPV6/11型阳性检出率为86.6%，HPV16/18型为16.6%，HPV31/33/35则为阴性，总的阳性检出率为90%。阳性结果作见于表皮浅层外，还见于棘细胞层中下部及基底细胞层内，作者认为原位分子杂交法是目前检测HPV感染及其分型的一种敏感、特异、快速且相对简便的方法，又能进行感染的组织学定位，同时这一方法还适应于对以往病例进行回顾性调查。     

Verrucous carcinoma of the anus containing human papillomavirus type 16 DNA detected by in situ hybridisation: a case report.

Verrucous carcinoma of the anus is a distinct and rare variant of well-differentiated squamous cell carcinoma. A case of anal verrucous carcinoma in a 35 year old man was studied by light microscopy and in situ DNA hybridization. Human papillomavirus (HPV) type 16 DNA sequences were demonstrated in the tumour cells, as well as in cells showing koilocytotic and dyskeratotic changes. The possible viral (HPV) aetiology of verrucous carcinoma is considered with a review of the previous literature.     

手术联合足叶草酯治疗肛周尖锐湿疣100例

我们于2003年1月至2008年7月采用手术联合足叶草酯治疗100例肛周尖锐湿疣患者,效果比较满意,现报道如下.     

Human papillomavirus types 16 and 39 in a vulval carcinoma occurring in a woman with Hailey-Hailey disease

A woman with Hailey-Hailey disease, suffering from carcinoma of the vulva, was examined by histology and for the presence of human papillomavirus (HPV) DNA by polymerase chain reaction (PCR) and in situ hybridization. Our diagnosis by histological examination revealed the vulval carcinoma to be a squamous cell carcinoma (SCC), adjacent to lesions of Hailey-Hailey disease and severe dysplasia/carcinoma in situ [vulval intraepithelial neoplasia (VIN) III]. The PCR with consensus primers for the L1 region (L1-PCR) successfully amplified HPV DNA using total DNA extracted from formalin-fixed and paraffin-embedded tissue specimens. Restriction fragment length polymorphism analysis and sequencing of L1-PCR products revealed HPV types 16 and 39. HPV 16-specific primers for the E6 region identified HPV 16 DNA. In situ hybridization analysis with biotinylated HPV 16 and 39 DNA probes revealed the presence of the HPV 39 genome in the nuclei of the tumour cells in the SCC. These results indicate that HPV 16 and 39 are associated with lesions in vulval carcinoma. Regarding the patient's susceptibility to infection in the case of Hailey-Hailey disease, there is a possibility that HPV was inoculated into the lesions of Hailey-Hailey disease and induced those of VIN III and SCC.