Anti-malarial herbal remedies of northeast India, Assam: an ethnobotanical survey

Ethnopharmacological relevance

Malaria is a serious public health problem in the north-eastern region of India including Assam, in view of development of chloroquine resistant Plasmodium falciparum. There is need for alternative and affordable therapy.

Aim of the study

This study was conducted to document indigenous knowledge, usage customs and practices of medicinal plant species traditionally used by the residents of Sonitpur district of Tezpur, Assam to treat malaria and its associated symptoms.

Materials and methods

A total of 50 randomly selected sampling represented by male (38.76%) and female respondents (12.24%) were interviewed using a semi-structured questionnaire.

Results

The present ethno-botanical survey revealed 22 species of plants belonging to 17 botanical families were reported to be used exclusively in this region for the treatment of malaria. Verbenaceae (three species), Menispermaceae (two species), and Acanthaceae (two species) botanical families represented the species that are most commonly cited in this survey work and the detailed use of plants has been collected and described.

Conclusions

The most serious threat to the existing knowledge and practice on traditional medicinal plants included cultural change, particularly the influence of modernization and lack of interests shown by the next younger generations were the main problems reported by the informants during the field survey. Hence, the proper documentation of traditional medicinal plants being used as anti-malarial agents and related indigenous knowledge held by the tribal community is an important approach to control the spread of vector-borne diseases like malaria reported in this survey work.     

An ethnopharmacological survey of the traditional medicine utilized in the community of Porvenir, Bajo Paraguá Indian Reservation, Bolivia

Ethnopharmacological relevance

Porvenir is a semi-isolated Indian community in the Bajo Paraguá Indian Reservation in Bolivian Amazon, one of the two communities of people from the Guarasug’we indigenous nation now close to extinction. The aim of our study was the collection of data on the traditional medicine utilized in the community, and to identify new subjects for further investigation by comparison of the folk-medicinal use with the available scientific literature data.

Materials and methods

Field work was conducted for 5 months, which included participant observation, semi-structured interviews with 16 individuals, and the collection of voucher specimens for botanical identification. The knowledge of the inhabitants relating to medicinal plants was analysed by means of the modified method of [Gentry and Phillips, 1993a] and [Gentry and Phillips, 1993b], which assesses the frequency and the variety of use of plants. Scientific data were gathered on selected species, and the correlations of the traditional uses of the herbs with scientific evidence were assessed.

Results

The lifestyle and beliefs in Porvenir, botanical data on the plants used, the frequency and variety of medicinal use, diseases that occur and their possible treatment, and methods of plant application are discussed in detail. 145 plant species were registered with 451 recorded uses. The majority of the plants were utilized to treat gastrointestinal complaints (60 species), followed by diseases of the central nervous system, pain and fever (37 species), diseases of the genitourinary tract (35 species), dermatological disorders (34 species) and diseases of the respiratory system (32 species). One fifth of the species are also applied in traditional medicine in other areas of Bolivia or in other countries. The majority of the 145 species used in the community have not been extensively investigated from phytochemical and pharmacological aspects. There are no data in the scientific literature on one fifth of the species.

Conclusions

The medicine applied in Porvenir and the contemporary knowledge of the people interviewed concerning plants reflect the local traditions and their changes very well, clearly demonstrating the influence exerted by conventional medicine, and how the ancestral knowledge is progressively being forgotten. The present ethnopharmacological survey indicates that 24 species that are frequently and consistently used in the community of Porvenir are perspective for further research, as their chemistry and pharmacology have not been published to date.     

Plants used to treat malaria in Nyakayojo sub-county, western Uganda

Aim of study

We performed an ethnobotanical study of plants used to treat malaria in Nyakayojo sub-county in south western Uganda because malaria in this region, and in Uganda at large, is still the single most important reason for ill health and mortality. Two of the most vulnerable groups affected by malaria are young children and pregnant women and plants are commonly used in their treatment.

Materials and methods

Twenty-eight traditional birth attendants were interviewed about how they used plants to treat malaria. Review of the literature available on all species identified was undertaken.

Results

Altogether 56 plant species were used by the informants, 48 of which have been identified to species level. Thirty-two (67%) of the species used by the respondents are documented for antimalarial use in other studies, and nearly half (44%) have documented anti-plasmodial activity. Fifty-five percent of the species were used by 2 or more of the respondents. The most commonly used species were Vernonia amygdalina, the indigenous Aloe species, Justicia betonica, Vernonia adoensis and Tithonia diversifolia. It was common to use more than one plant in a recipe (43%). The respondents had good knowledge of the symptom of malaria, and fairly good understanding of the causes.

Conclusion

The interviews show that the group of traditional birth attendants has an extensive and diverse knowledge on plants used in the treatment of malaria. The literature survey may indicate a possible explanation for the use of several plants.     

Effect and mechanism of fucoidan derivatives from Laminaria japonica in experimental adenine-induced chronic kidney disease

Ethnopharmacological relevance

Laminaria japonica is a popular seafood and medicinal plant in China, Chinese people use them as a traditional medicine for curing edema disease, a symptom of kidney diseases.

Materials and methods

The renal protective activity and mechanism of fucoidan derivatives was studied against adenine induced CKD in rats. The fucoidan derivatives were administered at dose of 50 and 150 mg/kg body weight. The serum urea nitrogen (SUN), serum creatinine (SCR) and some enzymic activity were detected.

Results

The SUN and SCR level decreased significantly with fucoidan derivatives administration. Histopathological changes of renal tubules and interstitium were markedly alleviated by fucoidan derivatives and the mesangial areas were also greatly reduced. Alterations were observed in the activities/levels of serum enzymic (CAT, GSH-PX) and non-enzymic (GSH) antioxidants, along with high level of malondialdehyde (MDA) in the CKD rats. However, normalized lipid peroxidation and antioxidant defenses occurred with fucoidan derivatives administration.

Conclusion

This study exhibited a new mechanism of fucoidan derivatives on CKD rats, that was the samples could alleviate renal tubules, interstitium and mesangial areas mediated by replacing the electronegative content of the glomerular cells and inhibition of mesangial cell proliferation. The study also proved the mechanism of fucoidan derivatives on the CKD rats had relationship with their antioxidant activities, the samples which could enhance the activity of antioxidant enzymes and reduce the LPO level could alleviate the symptom of CKD.     

Proliferative effects of five traditional Nigerian medicinal plant extracts on human breast and bone cancer cell lines

Ethnopharmacological relevance

The medicinal plants Hunteria umbellata (HUL), Cola lepidota (CCL), Persea americana leaf (PAL), Root bark of Persea americana (RPA) and Plukenetia conophora (PCL) are used in Nigerian traditional medicine for the treatment of cancer and cancer related diseases.

Aim of the study

To scientifically evaluate the cell proliferative and apoptotic effects of the plants extracts using breast and osteocarcinoma cell lines, and also to identify the possible components via LC-MS to have a kind of chemical fingerprint.

Materials and methods

The antiproliferative and apoptotic effects of methanolic extracts (10 μg/ml) of the five medicinal plants were subjected to in vitro evaluation using four cancer cell lines (breast-MCF-7 and BT-20; Osteocarcinoma-MG-63 and Saos-2) measured by flow cytometry. Non-tumorigenic controls MCF-12A and primary isolated osteoblasts (POB) were chosen to eliminate negative influence on healthy tissue.

Results

Of the five extracts RPA demonstrated a significant (P < 0.05) anti-proliferative activity against estrogen receptor positive breast cancer cell lines (MCF-7). The proliferative phase was decreased by 18%, whereas, a significant increase in cell proliferation (about 27%) was observed for RPA at a concentration of 10 μg/ml. PCL, CCL, HUL and PAL did not show marked inhibition of the proliferation of cell line MCF-7.

Conclusion

These results give suggestive evidence that the plant extracts exhibit some correlation between the claimed ethnomedicinal uses and the cell proliferative activity. RPA extract includes chemical compounds with estrogen-like activity and validates its potential use as anticancer agent, particularly against breast carcinoma; provided important information potentially helpful in drug designing and discovery. Further studies will involve the isolation of anti tumour compounds in RPA by LC-MS and detailed mechanism of anticancer activities.     

Ethnobotany and trade of medicinal plants in the Qaysari Market, Kurdish Autonomous Region, Iraq

Aim of study

Marketplaces epitomize a region's culture and trade, and can give a rapid insight into traditions and salience of commercialized medicinal products. The Qaysari bazaar, bordering the citadel in Erbil city in the Kurdistan Autonomous Region, Iraq, has 21 herbalist shops trading natural medicinal products, wild-crafted and cultivated from all over the Middle East and Asia Minor.

Materials and methods

Freelist surveys were conducted with 18 of these herbalists to determine diversity and salience of traded traditional medicinal plants. Interviews were conducted to document use, trade volume, origin, stock and value of the reported species. Plant species were identified using a combination of morphological identification and molecular barcoding using the ITS region.

Results

Vouchers were collected for a total of 158 samples, corresponding to 82 species of plants, 5 animal products, 8 types of stones, minerals or chemicals, as well as 16 mixtures of plant products. Consensus Analysis of the herbalist interviews shows strong support for a single culture of herbalist plant use.

Conclusions

Most reported plant species are known to have been used since antiquity, and uses are identical or similar to previously documented uses. Herbalists report a steady year-on-year increase in trade due to the economic stability in recent times. A majority (64%) of medicinal plants is imported from outside Iraq, and the data shows that imported plants trade at a higher price than locally-sourced species, and that these species are stocked in higher volumes by the herbalists to ensure a steady supply to consumers. A strong tradition of herbal medicine exists in Kurdistan today exemplified by the diverse and vigorous trade in medicinal plants commercialized from the provinces around Erbil to countries as far away as India, Spain and Libya.     

Mexican medicinal plants used for cancer treatment: pharmacological, phytochemical and ethnobotanical studies

Aim of the study

This review provides a summary of Mexican medicinal flora in terms of ethnobotanical, pharmacology, and chemistry of natural products related to anticancer activity.

Materials and methods

Bibliographic investigation was carried out by analyzing recognized books and peer-reviewed papers, consulting worldwide accepted scientific databases from the last five decades. Mexican plants with attributed anti-cancer properties were classified into six groups: (a) plant extracts that have been evaluated for cytotoxic effects, (b) plant extracts that have documented anti-tumoral effects, (c) plants with active compounds tested on cancer cell lines, (d) plants with novel active compounds found only in Mexican species, (e) plants with active compounds that have been assayed on animal models and (f) plants with anti-cancer ethnopharmacological references but without scientific studies.

Results

Three hundred plant species belonging to 90 botanical families used for cancer treatment have been recorded, of which only 181 have been experimentally analyzed. The remaining 119 plant species are in use in empirical treatment of diseases consistent with cancer symptomatology. Only 88 of the plant extracts experimentally studied in in vitro cellular models have demonstrated active cytotoxic effects in at least one cancer cell line, and 14 out of the 88 have also been tested in vivo with the results that one of them demonstrated anti-neoplasic effects. A total of 187 compounds, belonging to 19 types of plant secondary metabolites, have been isolated from 51 plant extracts with active cytotoxic effects, but only 77 of these compounds (41%) have demonstrated cytoxicity. Seventeen of these active principles have not been reported in other plant species. However, only 5 compounds have been evaluated in vivo, and 3 of them could be considered as active.

Conclusion

Clearly, this review indicates that it is time to increase the number of experimental studies and to begin to conduct clinical trials with those Mexican plants and its active compounds selected by in vitro and in vivo activities. Also, the mechanisms of action by which plant extracts and their active compounds exert anti-cancer effects remain to be studied.     

Plants used by native Amazonian groups from the Nanay River (Peru) for the treatment of malaria

Aim of the study

In order to evaluate the antimalarial potential of traditional remedies used in Peru, Indigenous and Mestizo populations from the river Nanay in Loreto were interviewed about traditional medication for the treatment of malaria.

Materials and methods

The survey took place on six villages and led to the collection of 59 plants. 35 hydro-alcoholic extractions were performed on the 21 most cited plants. The extracts were then tested for antiplasmodial activity in vitro on Plasmodium falciparum chloroquine resistant strain (FCR-3), and ferriprotoporphyrin inhibition test was also performed in order to assume pharmacological properties.

Results

Extracts from 9 plants on twenty-one tested (Abuta rufescens, Ayapana lanceolata, Capsiandra angustifolia, Citrus limon, Citrus paradise, Minquartia guianensis, Potalia resinífera, Scoparia dulcis, and Physalis angulata) displayed an interesting antiplasmodial activity (IC₅₀ < 10 μg/ml) and 16 remedies were active on the ferriprotoporphyrin inhibition test.

Conclusions

The results give scientific validation to the traditional medical knowledge of the Amerindian and Mestizo populations from Loreto and exhibit a source of potentially active plants.     

Antioxidant, toxicological and antiproliferative properties of Canadian polyphenolic extracts on normal and psoriatic keratinocytes

Aims of the study

In a first attempt for establishing the possible utilization of polyphenolic extracts from barks of Canadian wood species in psoriasis treatment, we aimed to study (a) their antioxidant capacity, (b) their toxicological properties on normal human keratinocytes (NHK), and (c) their effect on the growth of normal and psoriatic keratinocytes (PK).

Materials and methods

Polyphenolic extracts were obtained by 90% ethanolic maceration and hot water extraction (HWE). Scavenging capacity was evaluated towards hydrogen peroxide, hydroxyl, superoxide, nitric oxide and peroxyl radicals. MTT assay and Trypan blue dye exclusion (TBDE) method were used for evaluating the initial toxicity of the most antioxidant extracts on NHK during 24 and 48 h. The effects of extracts on the growth of NHK and PK at non-toxic concentrations were determined after exposure for 48 h.

Results

Yellow birch extract obtained by maceration (YB_Mac) and black spruce extract obtained by HWE (BS_HWE) were determined to have the highest antioxidant capacity, but BS_HWE was less toxic on NHK. Toxicity of extracts on keratinocyte plasma membrane and mitochondria after 24 h was attributed to their content of hydroxycinnamic acids and proanthocyanidins. BS_HWE inhibited the growth of NHK and non-lesional PK, but was not selective for lesional PK.

Conclusion

Given that BS_HWE presented elevated content of total phenols and flavonoids and showed a low toxicity on NHK as well as an adequate chemical reactivity towards different radicals and some antiproliferative properties, it was considered as the most valuable extract obtained from barks of Canadian wood species.     

Ethnomedicinal study of plants used by Sheko ethnic group of Ethiopia

Aim of the study

This study was conducted to document and evaluate knowledge on medicinal plant use by Sheko ethnic group in Southwest Ethiopia.

Materials and methods

Interviews and ranking exercises were the main methods employed to collect the ethnobotanical data. Fidelity level (FL) values were calculated for claimed Sheko medicinal plants to estimate their healing potentials.

Results

Seventy-one Sheko medicinal plants were reported, the majority of which were used to treat skin and gastro-intestinal ailments. Ocimum lamiifolium, Phytolacca dodecandra, Amaranthus dubius and Amaranthus graecizans were the medicinal plants assigned with the highest FL values, a possible indication of their better healing potential. The majority of Sheko medicinal plants were found to be herbs, and leaf was the most preferred plant part in remedy preparations. The study indicated that men, older people and illiterate ones had better knowledge of medicinal plants use as compared to women, younger people and literate ones, respectively.

Conclusions

The study showed that the Sheko people have rich knowledge of medicinal plant use. This knowledge is however, currently threatened mainly due to acculturation. Awareness should thus be created among Sheko community by concerned bodies regarding the usefulness of their medical practice. The efficacy and safety of the claimed medicinal plants need to be evaluated before recommending them for a wider use with priority given to those with high fidelity level values.     

Pharmaceutical ethnobotany in the Middle Navarra (Iberian Peninsula)

Ethnopharmacological relevance

This paper provides significant ethnobotanical information on pharmaceutical plant uses in the Middle Navarra (Iberian Peninsula). Collect, analyze and evaluate the ethnobotanical knowledge about medicinal plants in this area with 3622.2 km² and 404,634 inhabitants.

Materials and methods

We performed semi-structured interviews with 276 informants (mean age 72; 46% women, 54% men) in 111 locations, identified the plant reported and analyzed the results, comparing them with those from other territories.

Results

The informants reported data on 198 medicinal plants belonging to 60 botanical families. This work is focused on human medicinal plant uses, which represent 98% of the pharmaceutical uses (1401 use reports). The species with the highest number of cites are Santolina chamaecyparissus ssp. squarrosa, Jasonia glutinosa and Chamaemelum nobile with a long tradition of use in Navarra. All different plant parts are used; aerial part is exploited more frequently than other plant parts. Most of the listed remedies use a single ingredient, typically soaked in water. The most common mode of administration is oral, while the second most common is topical.

Conclusions

The main ailments treated are digestive troubles, wounds and dermatological problems, and respiratory affections. Informants reported 80 new or scarcely cited uses for 14 medicinal plants. For 36% of the species (5) we have not found bibliographical references in the scientific literature and 64% (9) have only one to three references.     

Procyanidin-rich fractions from Parkia biglobosa (Mimosaceae) leaves cause redox-sensitive endothelium-dependent relaxation involving NO and EDHF in porcine coronary artery

Aim of the study

Parkia biglobosa leaves are traditionally used as an antihypertensive agent in Benin. The present study assessed the vasorelaxant activity of different Parkia biglobosa leaf extracts using isolated porcine coronary artery rings.

Materials and methods

A hydroalcoholic leaf extract was submitted to a multi-step liquid-liquid fractionation with solvents of increasing polarity and the polyphenolic content of the different fractions was analyzed. Vascular reactivity of the different extracts was assessed using porcine coronary artery rings, in the presence or absence of specific pharmacological inhibitors.

Results

The hydroalcoholic, ethyl acetate and butanolic extracts contained mainly procyanidins and monomeric flavonoids. Parkia biglobosa leaf crude extract induced a redox-sensitive endothelium-dependent relaxation mediated by both nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF). The fractionation of the butanolic extract generated 6 fractions, two of which induced stronger vasorelaxation than the original extract and they had a higher phenolic content.

Conclusions

Parkia biglobosa leaf extract is able to induce endothelium-dependent NO- and EDHF-mediated relaxation in porcine coronary artery rings. The vasorelaxant activity is dependent on their phenolic content and appears to involve mainly procyanidins.     

The standardized extract of Loeselia mexicana possesses anxiolytic activity through the γ-amino butyric acid mechanism

Ethnopharmacological relevance

Loeselia mexicana (Lam.) Brand has been used in Mexican Traditional Medicine to treat “espanto” or “susto” (fear), which is a culturally affiliated syndrome whose symptomatology comprises loss of appetite, difficulty in sleeping, and also nausea and fatigue, with a sensation of fear or risk - real or imagined - to external stimuli.

Aim of the study

The anxiolytic effect of the standardized methanol extract of Loeselia mexicana, with regard to its content of coumarin daphnoretin, was researched utilizing the elevated plus maze (EPM) in order to demonstrate whether the biological effect produced by the plant is antagonized by drugs that block γ-amino butyric acid (GABA)ergic transmission.

Materials and methods

The methanolic extract of Loeselia mexicana (LmMeOH) was tested at different doses on the EPM and then the interaction of this extract was evaluated in the same model with different GABAergic drugs, such as flumazenil (FLU) 10 mg/kg, bicuculline (BIC) 5 mg/kg, pentylenetetrazole (PTZ) 10 mg/kg, and picrotoxin (PTX) 2 mg/kg. The effect of all of these treatments was evaluated by means of the open field test (OFT). Coumarin content was measured by the high performance liquid chromatography (HPLC) technique.

Results

The 200- and 400-mg/kg doses of methanolic extract containing 3.14 and 6.28 mg of daphnoretin, respectively, induced an anxiolytic effect in the EPM without modification of the spontaneous motor activity. The anxiolytic activity of 200 mg/kg of methanolic extract in EPM-exposed mice was antagonized by PTX, BIC, and FLU, but not by PTZ.

Conclusion

The data presented here indicate that the Loeselia mexicana Brand methanolic extract possesses a significant anxiolytic effect that appears to be mediated in part by activation of the GABAergic system.     

Inhibitory effect of hydro-methanolic extract of seed of Holarrhena antidysenterica on alpha-glucosidase activity and postprandial blood glucose level in normoglycemic rat

Ethnopharmacological relevance

The present experiment was conducted to search out the effect of hydro-methanolic extract of seed of Holarrhena antidysenterica on intestinal α-glucosidase activity in dose dependent manner and on the management of postprandial hyperglycemia in starch loaded rats.

Materials and methods

Activity of intestinal α-glucosidase was measured by in vitro method. Fasting blood glucose level was determined by single touch glucometer. Total phenol and flavonoids of seed extract of Holarrhena antidysenterica were estimated using gallic acid and quercetin standard curves, respectively.

Results

The degree of elevation in blood glucose level after starch administration was significantly (p < 0.05) less by the extract in respect to the control. The said extract also inhibited α-glucosidase activity having an IC₅₀ of 0.52 mg/ml. Phytochemical study revealed that the extract is rich in phenolic compounds (60.23 mg of gallic acid equivalent/g of extract) and flavonoids (360.23 mg of quercetin equivalent/g of the extract).

Conclusion

The extract exerts its antihyperglycemic effect by retarding the carbohydrate absorption from intestine through the inhibition in α-glucosidase activity and therefore resists postprandial hyperglycemia.     

Current applications of molecular imaging and luminescence-based techniques in traditional Chinese medicine

Ethnopharmacological relevance

Traditional Chinese medicine (TCM), which is fundamentally different from Western medicine, has been widely investigated using various approaches. Cellular- or molecular-based imaging has been used to investigate and illuminate the various challenges identified and progress made using therapeutic methods in TCM. Insight into the processes of TCM at the cellular and molecular changes and the ability to image these processes will enhance our understanding of various diseases of TCM and will provide new tools to diagnose and treat patients.

Materials and methods

Various TCM therapies including herbs and formulations, acupuncture and moxibustion, massage, Gua Sha, and diet therapy have been analyzed using positron emission tomography, single photon emission computed tomography, functional magnetic resonance imaging and ultrasound and optical imaging. These imaging tools have kept pace with developments in molecular biology, nuclear medicine, and computer technology.

Results

We provide an overview of recent developments in demystifying ancient knowledge - like the power of energy flow and blood flow meridians, and serial naturopathies - which are essential to visually and vividly recognize the body using modern technology.

Conclusions

In TCM, treatment can be individualized in a holistic or systematic view that is consistent with molecular imaging technologies. Future studies might include using molecular imaging in conjunction with TCM to easily diagnose or monitor patients naturally and noninvasively.     

Ethnobotanical study on some Malaysian anti-malarial plants: A community based survey

Ethnopharmacological relevance

Various plants species are used in the traditional medicine for the treatment of malaria. This is the first community based ethnobotanical study in Peninsular Malaysia.

Aim of the study

To investigate the plants traditionally used in the treatment of malaria in Malaysia.

Materials and methods

An ethnobotanical survey was carried out among 233 Aboriginal and rural households, and traditional healers in malaria endemic areas in Peninsular Malaysia. Data were collected using a pre-tested questionnaire.

Results

Nineteen species belonging to 17 families were identified. Twelve plant species have not previously been documented for the treatment of malaria in Malaysia.

Conclusions

Findings of this study can be used as an ethnopharmacological basis for selecting plants for further anti-malarial phytochemical and pharmaceutical studies.