共查询到10条相似文献,搜索用时 15 毫秒
1.
Christophe Nich Arnaud Marchadier Laurent Sedel Hervé Petite Catherine Vidal Moussa Hamadouche 《Journal of orthopaedic research》2010,28(2):178-183
Postmenopausal osteoporosis is a common disorder that results from increased osteoclastic activity caused by estrogen deficiency. Whether postmenopausal bone remodeling can alter the response to particulate debris is unknown. The purpose of this study was to evaluate the bone response to polyethylene particles in an ovariectomized murine model. Polyethylene particles were implanted onto the calvaria of seven control mice and seven ovariectomized (OVX) mice, as compared with calvaria from sham‐operated and OVX mice. Calvaria were harvested after 14 days. Skulls were analyzed with a high‐resolution micro‐CT and by histomorphometry after staining with Stevenel blue and picrofuschine, and for tartrate‐specific alkaline phosphatase. As assessed by micro‐CT, particle implantation induced a significant decrease in bone thickness in control mice, while bone thickness remained stable in OVX mice. In particle‐implanted animals, the osteoclast number was 2.84 ± 0.3 in control mice and 1.74 ± 0.22 in OVX mice. Mean bone loss was ?12% ± 1.9% in control mice and ?4.7% ± 1.7% in OVX animals. The reduction of osteolytic response suggests that ovariectomy may have a protective role against particle‐induced bone resorption. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:178–183, 2010 相似文献
2.
Antioxidant impregnated ultra‐high molecular weight polyethylene wear debris particles display increased bone remodeling and a superior osteogenic:osteolytic profile vs. conventional UHMWPE particles in a murine calvaria model 下载免费PDF全文
Yu Chen Nadim J. Hallab Yen‐Shuo Liao Venkat Narayan Edward M. Schwarz Chao Xie 《Journal of orthopaedic research》2016,34(5):845-851
3.
N. W. Struthers M. B. 《Urological research》1976,4(3):107-109
Summary The association of ureteric dilatation and urinary infection has been attributed to a direct toxic effect of E. Coli endotoxin on ureteric muscle. A specific causal relationship could not be established in this study in dogs. 相似文献
4.
Ichiro Kawahara Masanori Koide Osamu Tadokoro Nobuyuki Udagawa Hiroaki Nakamura Naoyuki Takahashi Hidehiro Ozawa 《BONE》2009,45(5):980-986
In the process of bone resorption, calcium is considered to be transported within vesicles in osteoclasts and eventually released. We studied the ultramicromorphology of calcium (Ca) transport in osteoclasts by preparing samples of osteoclasts collected from rat femurs in which calcium was maximally preserved and subjected them to high-pressure quick-freezing and freeze-substitution. We then examined the localization of calcium by Electron Energy Loss Spectroscopy (EELS). The structures of cell membranes were preserved, suggesting the suitability of this high-pressure quick-freezing and freeze-substitution technique.Osteoclast mitochondria adjacent to the ruffled border were rich in mitochondrial granules and contained a large amount of Ca. In contrast, mitochondria in the basolateral region contained few granules. Moreover, by an osteoclast-culturing experiment, differences in the morphology of mitochondrial granules were noted between culturing on a dentin slice and that on a gold plate, i.e., few mitochondrial granules were noted in osteoclasts cultured on a non-dentin plate.These findings suggest an association between the morphology of mitochondrial granules in osteoclasts and bone resorption as well as a new transport route for Ca resorbed by osteoclasts. We propose that Ca accumulates in mitochondria granules to prevent increased Ca concentration in cytoplasm of osteoclasts during bone resorption. 相似文献
5.
E W Taylor U Rajgopal J Festekjian 《JSLS, Journal of the Society of Laparoendoscopic Surgeons》2000,4(2):109-116
BACKGROUND AND OBJECTIVES: Endoscopic retrograde cholangiopancreaticography has been reported to have a high success rate in the detection and treatment of choledocholithiasis. Although there is growing enthusiasm for laparoscopic common bile duct clearance, many patients who present with gallbladder disease and suspected choledocholithiasis have endoscopic retrograde cholangiopancreatography performed with choledocholithiasis cleared if detected. These patients are then referred for laparoscopic cholecystectomy. The purpose of this study is to determine the efficacy of preoperative endoscopic retrograde cholangiopancreatography in the diagnosis and clearance of bile duct stones at our institution. METHODS: A retrospective review was performed of all patients at this institution who underwent preoperative endoscopic retrograde cholangiopancreatography for suspected choledocholithiasis followed by laparoscopic cholecystectomy from January 1997 through July 1998. RESULTS: Common bile duct stones were detected endoscopically in 12 of 17 (71%) patients. We found serum bilirubin level to be the best predictor of choledocholithiasis. In 12 of 12 procedures, the endoscopist performed an endoscopic sphincterotomy with stone extraction and reported a fully cleared common bile duct. Intraoperative cholangiogram performed during subsequent cholecystectomy revealed choledocholithiasis in 4 of these 12 patients. Laparoscopic techniques successfully cleared the choledocholithiasis in 3 of these patients with open techniques necessary in the fourth. CONCLUSIONS: Our data suggests that even after presumed successful endoscopic clearance of the bile duct stones, many patients (33% in our series) still have choledocholithiasis present at the time of cholecystectomy. We recommend intraoperative cholangiography at the time of cholecystectomy even after presumed successful endoscopic retrograde cholangiopancreatography with further intervention, preferably laparoscopic, to clear the choledocholithiasis as deemed necessary. 相似文献
6.
目的 通过分析2型糖尿病(T2DM)患者体内铁储量与骨密度的相关性,探讨铁蓄积对T2DM患者骨质疏松症患病风险的独立影响。方法 回顾性分析2018年12月1日至2020年12月31日在江苏大学附属昆山医院内分泌科住院治疗的T2DM 患者,收集患者的基本资料包括年龄、体质指数、腰椎和股骨颈骨密度、血清铁蛋白以及血脂等生化指标。首先对T2DM患者骨密度的潜在影响因素进行单因素分析,然后通过分层分析和曲线拟合探究不同性别T2DM患者中血清铁蛋白与骨密度的变化关系。最后通过多元回归模型调整混杂因素,分析血清铁蛋白对T2DM患者骨质疏松症患病风险的影响。结果 本研究共纳入411名T2DM患者(188名女性,223名男性)。T2DM女性和男性患者体内血清铁蛋白的中位浓度分别为139.65 μg/L和243.10 μg/L,两者骨质疏松患病率分别为38.83 %和25.56 %。单因素分析显示T2DM患者血清铁蛋白浓度与骨密度成负相关;曲线拟合显示,T2DM患者的骨密度随着血清铁蛋白浓度的升高而降低。多元回归分析显示,在调整混杂因素后,T2DM女性和男性患者中血清铁蛋白高浓度组患骨质疏松症的风险分别是低浓度组的17.163和3.732倍(P<0.05)。结论 T2DM患者中铁储量与不同部位骨密度之间存在负性关系。血清铁蛋白浓度上升是T2DM患者骨密度下降的独立危险因素,铁蓄积可能会增加T2DM患者骨质疏松患病率的风险。 相似文献
7.
A. Westerlind L. E. Larsson J. Häggendal B. Ekström-Jodal 《Acta anaesthesiologica Scandinavica》1995,39(4):467-471
After an intravenous injection of E. coli endotoxin in dogs a decrease in cerebral blood flow (CBF) and an increase in cerebral metabolic rate of oxygen (CMRo2 ) have been shown to occur. In metabolic acidosis following endotoxin CMRo2 increased with decreasing pH. A possible explanation for the increased CMRo2 after endotoxin and metabolic acidosis seems to be a damage of the blood-brain barrier (BBB) by endotoxin. This gives possibilities for a leakage of hydrogen ions and circulating monoamines from the blood to the brain, thus affecting the cerebral blood flow and metabolism.
The effects of an E. coli endotoxin injection on CBF and CMRo2 during metabolic acidosis and P-adrenoceptor blockade were studied in eight anaesthetized dogs. All the dogs were pretreated with propranolol (PPL), per os 12.5 mg . kg-1 twice a day for one week. Metabolic acidosis (pH 7.01–7.30) was achieved by an intravenous infusion of hydrochloric acid. Endotoxin E. coli lipopolysaccharide O 111:B 4 was given as an intravenous injection of 1 mg . kg-1 bodyweight over a 5 min period. Another five animals, published earlier, with the same experimental protocol but without PPL, constituted a control group.
After endotoxin no increase in CMRo2 or CBF was observed with increasing acidosis in the PPL-group. In the control group, after endotoxin, both CBF and CMR02 increased with decreasing pH. This resulted in a significant difference in both CBF and CMRo2 between the groups in the pH range 7.01—7.15.
The present results indicate that the increase in CMRo2 and CBF with metabolic acidodis in endotoxinaemia is mediated via P-adrenoceptors. 相似文献
The effects of an E. coli endotoxin injection on CBF and CMRo
After endotoxin no increase in CMRo
The present results indicate that the increase in CMRo
8.
Lanzafame RJ Stadler I Kurtz AF Connelly R Peter TA Timothy PA Brondon P Olson D 《Lasers in surgery and medicine》2007,39(6):534-542
BACKGROUND: Energy density and exposure time reciprocity is assumed and routinely used in low-level light therapy (LLLT) regimens. This study examined dose reciprocity effects on wound healing. METHODS: Pressure ulcers were created on seven groups of C57/BL mice (n = 18). Photoradiation was administered (18 days; 5 J/cm(2)/day @ 670 nm) using a custom LED apparatus and treatment matrix varying both intensity and exposure. Control animals were treated similarly, without photoradiation. Ulcer staging was performed using a standardized scale. Changes in stage, wound area and wound closure rates were measured. Histology was performed. RESULTS: Photostimulatory effects at day 7 occurred with parameters of 125 seconds @ 40 mW x 1/day; 625 seconds @ 8 mWx1/day; 62.5 seconds @ 40 mWx2/day; and 312.5 seconds @ 8 mWx2/day; and at day 18 using 625 seconds @ 8 mW and 312.5 seconds @ 8 mWx2/day. Statistically significant increases in wound closure rates occurred using 625 seconds @ 8 mW; 62.5 seconds @ 40 mWx2/day; and 312.5 seconds @ 8 mWx2/day treatments. Mean ulcer grade scores were similar to controls. CONCLUSIONS: Varying irradiance and exposure time to achieve a specified energy density affects phototherapy outcomes in this model. Variation of exposure time and irradiance may account for conflicting results in the literature. Further studies of these effects are warranted. 相似文献
9.
The role of the liver in the changes of acid-base balance and plasma lipids in surface-induced deep hypothermia for open-heart
surgery was investigated clinically and experimentally. The metabolic acidosis generally observed in open-heart surgery under
surface-induced deep hypothermia is derived from lactacidemia. Although the accelerated anaerobic glycolysis is partly responsible,
the depressed acidic metabolite-disposing ability of the liver also plays an important role. The evidence which is presented
to show the decrease of plasma lipids other than NEFA under hypothermia is probably due to the hepatic accumulation of lipids
and the decreased lipids release from the liver. 相似文献
10.
Crosstalk between substance P and calcitonin gene‐related peptide during heterotopic ossification in murine Achilles tendon 下载免费PDF全文
Ceren Tuzmen Kostas Verdelis Lee Weiss Phil Campbell 《Journal of orthopaedic research》2018,36(5):1444-1455