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1.
人白细胞介素10(hIL—10)基因的克隆和序列测定 总被引:1,自引:0,他引:1
应用逆转录多聚酶链式反应(RT-PCR)技术,自行设计并合成一对引物,成功地从活化的正常人外周血单个核细胞中扩增出hIL-10cDNA基因。并定向插入pUC18载体,经DNA序列测定最终确定为hIL-10cDNA基因,这将为今后hIL-10基因探针的制备和基因表达,进一步在基因水平上探讨hIL-10的生物学功能提供了物质基础。 相似文献
2.
中国旱獭α干扰素家族基因的克隆及序列分析 总被引:3,自引:0,他引:3
目的 克隆中国旱獭α干扰素(IFN-α)家族基因,以期利用克隆的中国旱獭家族IFN-α在动物模型上探索慢性乙型肝炎有效的干扰素治疗方案和策略。方法 利用分子克隆技术对中国旱獭IFN-α家族基因进行克隆,并对所克隆的系列基因进行测序、分型、系统发生树分析、同源性比较及理化特性分析。结果从112个中国旱獭IFN-α基因克隆中获得18个独立的不重复序列,其中的14个序列来自4次以上相对独立的PCR产物,将它们分为14个基因亚型,其中8个是功能基因亚型,6个是假基因亚型。中国旱獭IFN-α各基因亚型之间在核苷酸水平和氨基酸水平有很高的同源性,平均分别为93%和85%,前体蛋白N端含23个氨基酸的疏水性信号肽。结论 中国旱獭巧IFN-α家族至少有14个基因亚型,这些基因的克隆可能应用于中国旱獭HBV动物感染模型,进行干扰素基因治疗和研究干扰素治疗策略。 相似文献
3.
应用逆转录多聚酶链式反应(RT-PCR)技术,自行设计并合成一对引物,成功地从活化的正常人外周血单个核细胞中扩增出hIL-10cDNA基因。并定向插入pUC18载体中,经DNA序列测定最终确定为hlL-10cDNA基困,这将为令后hIL-10基因探针的制备和基因表达,进一步在基因水平上探讨hIL-10的生物学功能提供了物质基础。 相似文献
4.
目的:探讨孟鲁司特在支气管哮喘患者体内IL-6、IL-8和IL-10水平的影响。方法:应用放射免疫分析和酶联法对31例支气管哮喘患者应用孟鲁司特治疗前后血清IL-6、IL-8和IL-10水平的变化,并与35名正常健康人作比较。结果:支气管哮喘患者在治疗前血清IL-6、IL-8水平非常显著地高于正常人组(P<0.01),而IL-10水平显著地低于正常人组(P<0.01),经治疗2周后与正常人组比较仍有显著性差异(P<0.05)。结论:孟鲁司特对支气管哮喘患者血清IL-6、IL-8和IL-10有一定程度的调节作用,从而降低患者体内的炎症水平,促进病情缓解和好转。 相似文献
5.
丙型肝炎患者血清TNF,IL-6,IL-10及其临床意义 总被引:9,自引:9,他引:0
目的 :探讨了丙型肝炎患者血清中TNF、IL - 6、IL - 10水平及意义。方法 :分别应用ELISA法和放免法检测了 5 8例丙型肝炎患者血清中TNF、IL - 6、IL - 10水平 ,并与 35名正常健康人作比较。结果 :丙型肝炎患者血清中TNF、IL - 6、IL - 10水平均非常显著地高于正常人水平 (P <0 .0 1) ,肝硬化组为甚 ,且TNF与IL - 6、IL - 10呈正相关 (r =0 .6 135 ,0 .6 2 2 5 ,P <0 .0 1)。结论 :TNF、IL - 6、IL - 10在丙型肝炎病毒感染的致病机理中有一定的临床价值。 相似文献
6.
目的:探讨牙周病患者血清hs-CRP、IL-8和IL-10水平的变化及临床意义。方法:应用放射免疫分析、酶联法和免疫比浊法对32例牙周病患者进行了血清hs-CRP、IL-8和IL-10检测,并与35名正常人作比较。结果:牙周病患者在治疗前血清hs-CRP、IL-8和IL-10水平均非常显著地高于正常人组(P〈0.01),经治疗1个月后与正常人组比较仍有显著性差异(P〈0.05)。结论:检测血清hs-CRP、IL-8和IL-10水平的变化,对探讨牙周病发病机制、预防和指导治疗均有一定的临床价值。 相似文献
7.
消化性溃疡患者治疗前后血清Gas、IL-6和IL-10检测的临床意义 总被引:2,自引:0,他引:2
目的:探讨了消化性溃疡患者治疗前后血清胃泌素(Gas)、白细胞介素-6(IL-6)和白细胞介素-10(IL-10)水平的变化及意义。方法:应用放射免疫分析对61例消化性溃疡患者进行了治疗前后血清Gas、IL-6和IL-10水平的检测,并与35名正常健康人作比较。结果:消化性溃疡患者在治疗前血清Gas、IL-6和IL-10水平均非常显著地高于正常对照组(P〈0.01),经治疗3个月后则与正常对照组比较无显著性差异(P〉0.05)。结论:检测消化性溃疡患者血清Gas、IL-6和IL-10水平的变化可作为病情及预后评估的重要检测指标。 相似文献
8.
目的:详述肝硬化患者血清TGF-β1、IL-10、IL-12和IL-18水平的变化及临床意义。方法:应用酶联法对66例肝硬化患者(其中30例为肝硬化腹腔积液组,36例为肝硬化无腹腔积液组)进行了血清TGF-β1、IL-10、IL-12和IL-18测定并与35例正常人作比较。结果:肝硬化患者血清TGF-β1、IL-10、IL-12和IL-18水平均非常显著地高于正常人组(P〈0.01),尤以肝硬化腹腔积液组为甚。肝硬化患者血清TGF-β1水平与IL-10、IL-12和IL-18水平呈正相关(r=0.5018、0.5742、0.6011,P〈0.01)。结论:检测肝硬化患者血清TGF-β1、IL-10、IL-12和IL-18水平的变化有助于对疾病变化过程和治疗功效的评估,因而可提供重要的临床价值。 相似文献
9.
中国人IL—18结构蛋白cDNA的克隆和序列分析 总被引:6,自引:1,他引:5
目的克隆人白介素 - 18结合蛋白 (h IL- 18BP)的 c DNA,以研究其结构与功能的关系。方法从中国汉族人的胎盘组织中提取总 RNA,以 RT- PCR方法扩增出全长 5 85个 bp的 IL- 18BP c DNA,将其与表达载体 pc DNA3连接 ,转化大肠杆菌 DH5 α,建立了 h IL- 18BP的 c DNA克隆。结果序列分析表明 ,与国外文献报道的人 IL- 18BP的 c DNA序列完全一致。结论 h IL- 18BP已成功地得到克隆。 相似文献
10.
目的:探讨了消化性溃疡患者输注悬浮红细胞前后Gas、IL-2、IL-10和IL-18水平的变化及临床意义.方法:应用放射免疫分析和酶联法对31例消化性溃疡患者输注悬浮红细胞前后Gas、IL-2、IL-10和IL-18进行了检测,并与35名正常健康人做比较.结果:消化性溃疡患者在治疗前血清IL-2水平显著低于正常人组(P... 相似文献
11.
Fleming SB Haig DM Nettleton P Reid HW McCaughan CA Wise LM Mercer A 《Virus genes》2000,21(1-2):85-95
Orf virus is a large DNA virus and is the type species of the Parapoxvirus genus of the family Poxviridae. Orf virus infects the epithelium of sheep and goats and is transmissible to humans. Recently we discovered a gene in orf virus that encodes a polypeptide with remarkable homology to mammalian interleukin (IL-10) and viral encoded IL-10s of herpes viruses. The predicted polypeptide sequence shows high levels of amino acid identity to IL-10 of sheep (80%), cattle (75%), humans (67%) and mice (64%), as well as IL-10-like proteins of Epstein-Barr virus (63%) and equine herpes virus (67%). The C-terminal region, comprising two-thirds of the orf virus protein, is identical to ovine IL-10 which suggests that this gene has been captured from its host sheep during the evolution of orf virus. In contrast the N-terminal region shows little homology with cellular IL-10s and in this respect resemble other viral IL-10s. IL-10 is a pleiotrophic cytokine that can exert either immunostimulatory or immunosuppressive effects on many cell types. IL-10 is a potent anti-inflammatory cytokine with inhibitory effects on non-specific immunity in particular macrophage function and Th1 effector function. Our studies so far, indicate, that the functional activities of orf virus IL-10 are the same as ovine IL-10. Orf virus IL-10 stimulates mouse thymocyte proliferation and inhibits cytokine synthesis in lipopolysaccharide-activated ovine macrophages, peripheral blood monocytes and keratinocytes. Infection of sheep with an IL-10 deletion mutant of orf virus has shown that interferon- levels are higher in tissue infected with the mutant virus than the parent virus. The functional activities of IL-10 and our data on orf virus IL-10 suggest a role in immune evasion. 相似文献
12.
Gui-Liang Shi Xing-Xing Cai Ya-Min Su Chu Chen Xin-Tao Deng Hai-Yan Pan Meng-Kan Fan Jian-Hua Zhu Min Pan 《International journal of clinical and experimental pathology》2015,8(4):4091-4098
An accumulating body of evidence suggests that slow coronary flow (SCF) phenomenon seems to be an early-form of atherosclerosis and low-grade inflammation plays a major role in the atherosclerotic vascular processes. Interleukin (IL)-10 is a multifunctional cytokine involved in both innate and adaptive immune response. The aim of the present study is to investigate the association of IL-10 gene -592A/C polymorphism with SCF in Han Chinese. 250 patients who underwent coronary angiography and had angiographically normal coronary arteries of varying coronary flow rates without any atherosclerotic lesion were enrolled in this study. Patients who had thrombolysis in myocardial infarction frame counts (TFC) above the normal cutoffs were considered to have SCF and those within normal limits were considered to have normal coronary flow (NCF). The PCR-based restriction fragment length polymorphism (PCR-RFLP) technique was used to assess the genotypes frequencies. The distribution of the IL-10 -592A/C genotypes (AA, AC, and CC) was 46.34%, 41.46%, and 12.20% in the NCF group, and 66.51%, 28.71%, and 4.78% in SCF subjects, respectively (P = 0.0280). The frequency of the A allele in the SCF group was significantly higher than that in the NCF group (80.86% vs. 67.07%, P = 0.0054). Compared with the CC genotype, the AA genotype had increased risk of SCF in both unadjusted and adjusted analyses. In SCF patients, the average serum IL-10 levels in AA genotype were statistically lower than in AC + CC genotype (P = 0.0000). These findings suggest that IL-10 -592A/C polymorphism is associated with SCF and the A allele has increased risk for SCF in Han Chinese. 相似文献
13.
充血性心力衰竭患者血清白介素-10水平变化及意义 总被引:2,自引:0,他引:2
目的通过检测充血性心力衰竭(CHF)患者血清白介素-10(IL-10)水平,探讨其与CHF的关系及临床意义。方法采用双抗体夹心法测定30例CHF息者治疗前后及25例健康人血清IL-10水平。结果CHF患者血清IL-10水平显著高于时照组(P〈0.01),随CHF加重,IL-10水平升高越明显(心功能Ⅱ级与对照组比较差异不显著P〉0.05,Ⅲ级及Ⅳ级与对照组比较P〈0.01):心衰好转后明显下降(P〈0.01)。结论 IL-10可能参与了CHF的发生和发展,其在临床方面的意义有待于进一步探讨。 相似文献
14.
背景:既往研究发现接合性质粒pRST98可促进其宿主菌生物膜的形成,携带pRST98的菌株感染细胞和实验动物后,可促进白细胞介素10的分泌和表达。
目的:体外研究不同质量浓度白细胞介素10与接合性质粒pRST98对鼠伤寒沙门菌生物膜形成的相互影响。
方法:体外建立鼠伤寒沙门菌标准株χ3306、突变株χ3337及接合株χ3337/pRST98 3组生物膜模型,3组分别加入0(空白对照),1,10,100 µg/L白细胞介素10,通过结晶紫染色半定量法、共聚焦激光扫描显微镜分析和扫描电镜观察,比较不同质量浓度白细胞介素10对携带和不携带接合性质粒pRST98的鼠伤寒沙门菌生物膜形成的影响。
结果与结论:①3组组内比较:与空白对照组比较,1,10 µg/L白细胞介素10均能促进鼠伤寒沙门菌的聚集,提高生物膜形成能力,且10 µg/L质量浓度效果更明显;100 µg/L白细胞介素10抑制鼠伤寒沙门菌生物膜的形成。②3组组间比较:在同一白细胞介素10质量浓度下,接合株χ3337/pRST98组A570高于标准株χ3306组、突变株χ3337组。结果说明1,10 µg/L白细胞介素10可促进生物膜的形成,且在携带接合性质粒pRST98的情况下促进作用更明显。
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程全文链接: 相似文献
15.
Genetic factors are known to be important in the development of nasopharyngeal carcinoma (NPC). Interleukin-10 (IL-10) is an immunosuppressive cytokine which may facilitate development of cancer by supporting tumor escape from the immune response. Interindividual variations in IL-10 production were genetically contributed to polymorphisms within IL-10 promoter region. The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) at positions -1082 (A/G), -819 (T/C) and -592 (A/C) in the IL-10 gene promoter were involved in predisposing an individual to NPC. One hundred and ninety-eight patients with NPC and 210 age- and sex-matched controls, genotypes were determined using polymerase chain reaction-restriction fragment length polymorphism. There were significantly differences in the genotype and allele distribution of -1082 A/G polymorphism of the IL-10 gene among cases and controls. The -1082 AG and GG genotypes were associated with a significantly increased risk of NPC as compared with the -1082 AA genotypes. Haplotype analysis showed that the homozygosity of the GCC haplotype (defined by SNPs at positions -1082, -819 and -592) of IL-10 gene conveys the highest risk for NPC compared with the homozygosity for the ATA haplotype. This study shows for the first time an association between IL-10 gene promoter -1082 A/G polymorphism and its haplotype may contribute to genetic susceptibility to NPC in a Chinese population. 相似文献
16.
Eli Geva Joseph B. Lessing Liat Lerner-Geva Foad Azem Israel Yovel Dalit Ben-Yosef Uriel Barkai Ami Amit 《American journal of reproductive immunology (New York, N.Y. : 1989)》1997,37(2):187-190
PROBLEM: The present study aimed at investigating the presence of Interleukin-10 (IL-10) in preovulatory follicular fluid (FF) and its possible correlation with 17-β-estradiol (E2) and progesterone (P) levels, and treatment outcome in patients undergoing in-vitro fertilization-embryo transfer (IVF-ET). METHODS: Twenty consecutive patients with tubal factor infertility who underwent oocyte retrieval for IVF-ET were assayed for pooled, preovulatory FF levels of IL-10, E2, and P. RESULTS: The mean FF levels of IL-10, E2, and P were 78.7 ± 104.7 pg/ml, 2,787.0 ± 726.1 pg/ml, and 1.5 ± 0.8 ng/ml, respectively. No correlation was found between preovulatory FF concentration of IL-10, E2, oocyte number, oocyte fertilization rate, embryo quality, and pregnancy rate. The levels of IL-10 were found to be negatively correlated with P concentration, although not significantly (P = 0.057). CONCLUSION: Interleukin-10 exists in the preovulatory FF. Further investigations are needed to determine the role of IL-10 in the folliculogenesis. 相似文献
17.
The nucleotide and deduced amino acid sequence of genome segment 11 encoding a nonstructural protein of an aquareovirus strain
SBR have been determined. Nucleotide sequence analysis showed that the genome segment 11 of SBR virus is 780 nucleotides long
and contains a major open reading frame that codes for a polypeptide of 236 amino acids with a predicted molecular weight
of 25,504 Da. The second reading frame of genome segment 11 was 480 nucleotides long and codes for a polypeptide of 145 with
a predicted molecular weight of 15,715 Da. The genome segment 11 contains 24 nontranslated nucleotides at the 5′-end and 48
nontranslated nucleotides at the 3′-end. This gene codes for two nonstructural polypeptides NS29 and NS15. Comparison of the
deduced amino acid sequence of this gene with the published sequences of other members of the family Reoviridae indicated
no sequence relatedness.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
18.
19.
Interleukin-10- and corticosteroid-induced reduction in type I procollagen in a human ex vivo scar culture 总被引:5,自引:0,他引:5
A. WANGOO C. LABAN H.T. COOK B. GLENVILLE & R.J. SHAW 《International journal of experimental pathology》1997,78(1):33-41
Fibroblasts act as the effector cells of the fibrotic response via production of collagen. In an attempt to understand the regulation of fibroblasts from areas of active human tissue fibrosis, we have developed an ex vivo model in which biopsies of scars from patients 6 weeks post thoracotomy were cultured. This model has been used to investigate whether interleukin-10 (IL-10) and triamcinolone acetonide modulate the expression of type I procollagen mRNA and protein. In situ hybridization and a quantitative competitive RT-PCR were used to measure type I procollagen mRNA. Type I procollagen protein was evaluated by immunochemistry. Viability of biopsies in culture using 3 H-uridine incorporation into RNA was observed to be > 80% for at least 96 hours. Following addition of either IL-10 or triamcinolone acetonide there was a modest but significant decrease ( P <0.05) in type I procollagen mRNA expression. Similarly, each agent added individually to biopsies reduced the proportion of cells staining positively for type I procollagen when compared to biopsies treated with medium alone ( P <0.05). These results extend in vitro data that IL-10 and corticosteroids down-regulate collagen synthesis in skin fibroblast cell lines and suggest that this ex vivo model may offer a closer approximation to the post-operative scarring process when testing new therapeutic agents for reducing an over-exuberent fibrotic response. 相似文献
20.
V.J. Goodwin T.A. Sato M.D. Mitchell J.A. Keelan 《American journal of reproductive immunology (New York, N.Y. : 1989)》1998,40(5):319-325
PROBLEM: To determine the effects of anti-inflammatory cytokines on the production of inflammatory mediators by placental cells. METHOD OF STUDY: Cells from term human placentas were isolated and cultured in vitro in the presence of anti-inflammatory cytokines interleukin (IL)-4, IL-10, and transforming growth factor (TGF)-β. Their effects on the production of IL-8 and prostaglandin E2 (PGE2) were investigated under basal conditions and after stimulation with IL-1β and tumor necrosis factor (TNF)-α. RESULTS: Both IL-10 and IL-4 inhibited IL-1β- and TNF-α-induced PGE2 production but had no significant effects on the production of PGE2 under basal conditions. TGF-β1 was without effect in stimulated cells, whereas under basal conditions TGF-β1 stimulated PGE2 production. Similar trends were seen for IL-8 production, with the exceptions that TGF-β1 decreased the TNF-α-induced production and IL-4 decreased basal IL-8 production. CONCLUSIONS: The anti-inflammatory effects shown by IL-4, IL-10, and (to lesser extent) TGF-β may play a role in ameliorating the potentially harmful effects of pro-inflammatory mediators in the feto-placental unit. 相似文献