反相高效液相色谱法测定人血吗氯贝胺浓度

以乙腈－水（３０：７０，Ｖ／Ｖ）为流动相，氯霉素为内标，采用反相高效液相色谱法测定吗氯贝胺浓度，血吗氯胺在碱性条件下用乙醚提取，甲醇溶液残留物，在０．０４～４．０μｇ／ｍｌ，浓度范围内线性关系良好ｒ＝０．９９９６，相对平均回收率为９７．０％，日内ＲＳＤ（％）２．６～３．５日间ＲＳＤ（％）３．７～４．２，最低检出浓度０．０４μｇ／ｍｌ本法快速，准确，灵敏度高，专一性强，适用于吗氯贝胺临床监测及科研需     

高效液相色谱法测定舒他西林片中氨苄青霉素的血药浓度及药动学研究

采用ＨＰＬＣ－荧光检测法测定舒他西林片中氨苄青霉素的血药浓度,采用ＳｐｈｅｒｉｓｏｒｂＯＤＳ柱,流动相为磷酸盐缓冲液（０．０５ｍｏｌ／Ｌ,ｐＨ＝４．０）－乙腈（７８２２,Ｖ／Ｖ）,标准曲线范围０．２２～１３．８９μｇ／ｍｌ,ｒ＝０．９９９７,保留时间为７．３ｍｉｎ,回收率大于９５％,日内和日间差小于７％,最低检测浓度为０．２２μｇ／ｍｌ,无内源性杂质干扰。９名受试者的主要药动学参数：Ｔ１／２＝１．０４±０．２２ｈ,Ｃｍａｘ＝１２．３５±３．１３μｇ／ｍｌ,Ｔｍａｘ＝０．８３±０．４５ｈ,ＡＵＣ＝１９．４９±３．４６μｇｈ／ｍｌ。     

用HPLC法同时测定洗必泰及有关杂质对氯苯胺的研究

采用反相高效液相色谱法同时测定了洗必泰及有关杂质对氯苯胺浓度。用Ｚｏｒｂａｘ－Ｃ＿８柱，０．２ｍｏｌ／ＬＮａＨ＿２ＰＯ＿４（ｐＨ３．０）－甲醇（４９．５：５０．５）为流动相，非那西丁为内标，２４０ｎｍ波长检测。本法简便准确，重现性好。洗必泰和对氯苯胺分别在１５～２００μｇ／ｍｌ和６０～１２００μｇ／ｍｌ浓度范围内线性关系良好（ｒ＝０．９９９９），最低检测限分别为１．３３μｇ／ｍｌ和７．５μｇ／ｍｌ（Ｓ／Ｎ＝３：１），平均回收率分别为１００．１％和１０３．１％。相对标准偏差Ｒ５０％分别为１．４１％和１．１４％。     

高效液相色谱法测定舒他西林片中氨苄青霉素的血药浓度及 …

采用ＨＰＬＣ－荧光检测法测定舒他西林片中氨苄青霉素的血药浓度,采用Ｓｐｈｅｒｉｓｏｒｂ ＯＤＳ柱,流动相为磷酸盐缓冲液（０．０５ｍｏｌ／Ｌ,ｐＨ＝４．０）－乙膊（７８：２２,Ｖ／Ｖ）,标准曲线范围０．２２￣１３．８９μｇ／ｍｌ,ｒ＝０．９９９７,保留时间为７．３ｍｉｎ,回收率大于９５％。日内和日间差小于７％,最低检测浓度为０．２２μｇ／ｍｌ,无内涛性杂质干扰。９名受试者的主要药动学参数：Ｔ１／２＝     

RP－HPLC法测定血浆中替诺昔康的浓度

血浆样品用二氯甲烷提取,采用ＯＤＳ柱,以甲醇－磷酸盐缓冲液－三乙胺（３０：６９．８：０．２）为流动相,炎痛喜康为内标,２５４ｎｍ为检测波长,ＲＰ－ＨＰＬＣ法测定兔口服替诺昔康片后的体内血药浓度。线性范围０．１～３０μｇ／ｍｌ,最低检测用０．１μｇ／ｍｌ（Ｓ／Ｎ＞２）．萃取回收率８５．７１％,方法回收率９９．７０％～１１０．７％。     

HPLC法测定SHR血清中硝苯地平浓度及其药代动力学研究

目的建立血清中硝苯地平浓度的ＨＰＬＣ测定法，研究其在自发性高血压大鼠（ＳＨＲ）体内的药动学。方法ＳＨＲ灌胃给与硝苯地平１０ｍｇ／ｋｇ，用ＨＰＬＣ法测定硝苯地平血清浓度。用乙酸乙酯提取出血清中的硝苯地平，在５０℃水浴中用氮气吹干，残渣用流动相溶解后进样。选用ｓｈｉｍＰａｃｋＣＬＣ－ＯＤＳ柱，甲醇－水（６∶４）作流动相，检测波长２３５ｎｍ。结果：硝苯地平最低检出浓度为０．０２μｇ／ｍｌ，标准曲线线性范围为０．０２～５．０μｇ／ｍｌ（ｒ＝０．９９９７），血样平均回收率为９８．０９％。硝苯地平在大鼠体内的药动学符合一定开放模型，其参数为Ｔ１／２ｋａ＝０．８２８±０．５５ｈ，Ｔ１／２ｋｅ＝３．１７±２．３２ｈ，Ｔｍａｘ＝２．１２±１．４７ｈ，Ｃｍａｘ＝２．７８±１．５０μｇ／ｍｌ和ＡＵＣ＝２４．５±２９．２ｈ·μｇ／ｍｌ。结论本法能满足血清中硝苯地平浓度测定及药动学研究的需要。     

反相HPLC法快速测定人血清中抗坏血酸含量

应用ＵｌｔｒａｓｐｈｅｒｅＯｃｔｙｌ柱，建立了快速测定人血清中抗坏血酸含量的反相ＨＰＬＣ法。流动相为醋酸盐缓冲液（ｐＨ５．２５）。电化学检测器用碳膜电极和甘汞参比电极，工作电压＋０．６０Ｖ，灵敏度１００ｎＡ／Ｖ。抗坏血酸的平均回收率为１００．４６％，日内（ｎ＝５）和日间（ｎ＝８）的平均变异系数分别为３．８５％和６．８０％。最低检出浓度为０．１３６μｇ／ｍｌ，线性范围０．１３６μｇ／ｍｌ～１３．６μｇ／ｍｌ（ｎ＝９，ｒ＝０．９９９９）。     

荧光法检测血清硒与组织硒

本研究应用２．３－萘二胺（ＤＡＮ）与硒反应生成５，６－苯并韭硒脑的荧光分光光度法检测血清硒与组织硒，通过方法学的实验研究，认为本法线性范围宽（０．０５μｇ－１．５０μｇ／ｍｌ）ｒ为０。９９９，ＣＶ值为１．０９％（ｎ＝８）、回收率为９９．９７％，是一种较为灵敏、准确、重复性较好的定量检测法。我们对３０例正常健康人的血清硒及２４例正常健康人的乳腺组织测得其平均值分别为０．０７６±０．０１２μｇ／ｍｌ和１．３２３±０．４０μｇ／ｇ组织，与国内学者报导的基本一致。     

反相高效液相色谱法测定人血吗氯贝胺浓度

以乙腈水（３０∶７０， Ｖ／ Ｖ）为流动相，氯霉素为内标，采用反相高效液相色谱法测定吗氯贝胺浓度。血吗氯贝胺在碱性条件下用乙醚提取，甲醇溶解残留物，在 ００４～４．０μｇ／ｍ ｌ浓度范围内线性关系良好，ｒ＝ ０．９９９６，相对平均回收率为 ９７０％ ，日内 Ｒ Ｓ Ｄ（％ ）２６～３．５，日间 Ｒ Ｓ Ｄ（％ ）３．７～４．２，最低检出浓度００４μｇ／ｍ ｌ。本法快速、准确，灵敏度高，专一性强，适用于吗氯贝胺临床监测及科研需要。     

HPLC法测定血中茶碱浓度

本文建立了一种流动相为甲醇－乙腈－０．０１ｍｏｌ／Ｌ乙酸（７．５：７．６：８５）的ＨＰＬＣ法测定血清茶碱浓度。ＯＤＳＣ－１８反相色谱柱，柱温３０℃．流动相流途１．５ｍｌ／ｍｉｎ，ＵＶ／ｖｉｓ检测器，检测被长２７８ｎｍ，用外标沽定量。血浴样品用氯仿／异丙醇（９６：６）提取，水浴蒸干，甲醇６０μｌ重溶。茶碱保留时间约４．８０ｍｉｎ；标准曲线回归方程Ｙ＝０．６０８３＋０．３８６６ｘ，ｒ＝０．９９８１：茶碱浓度１０．０．２０．０．４０．０μｇ／ｍｌ时回收率分别为９４．７１±３．３５．１０８．４２±５．２２和９０．４８±４．３２：最低检测限０．３１２５μｇ／ｍｌ；日内变异系数３．１４％，日间变异系数４．７３％，结果显示，木法分离效果好。而且快速、简便。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.