高频喷射通气对单肺通气患者氧化应激反应的影响

目的 研究高频喷射通气(HFJV)对单肺通气患者氧化应激反应的影响.方法 择期行食管癌根治术患者45例,按随机数字表法分为双肺通气组(A组)、单肺通气组(B组)、单肺通气时非通气侧给予HFJV(驱动压力1 kg/cm2,频率100次/min)组(C组),每组15例.分别于开胸前(T0),单肺通气后(A组于开胸后)30 min(T1)、90 min(T2)、150 min(T3),手术结束时(T4)测定超氧化物歧化酶(SOD)活性及丙二醛(MDA)、一氧化氮(NO)浓度.结果 T2～T4时B、C组SOD活性[B组:(47±10)、(37±9)、(41±7)kU/L;C组:(58±12)、(51±11)、(49±9)kU/L]低于A组[(78±8)、(75±7)、(79±6)kU/L](P＜0.05),T1～T4时B、C组MDA、NO浓度高于A组(P＜0.05);T3时C组SOD活性明显高于B组(P＜0.05),T1～T4时C组MDA浓度及T2～T4时NO浓度低于B组(P＜0.05).结论 HFJV能在一定程度上抑制单肺通气患者的氧化应激反应.

Abstract：

Objective To determine the effects of high-frequency jet ventilation (HFJV) on oxidative stress in patients during one-lung ventilation (OLV). Methods Forty-five patients undergoing elective radical esophageal cancer resection were divided into three groups with 15 cases each by random digits table: two lung ventilation group ( group A), OLV group (group B), HFJV- OLV group ( group C, working pressure 1 kg/cm2 and frequency 100 times/min). Venous blood samples were taken before induction (T0),at30min (T1),90min (T2),150min (T3) after OLV and the end of operation (T4) for measuring serum superoxide dismutase(SOD),malondialdehyde (MDA) and nitric oxide (NO). Results SOD was lower at T2-T4 in group B[(47 ± 10), (37 ±9), (41 ±7) kU/L] and group C[(58 ± 12), (51 ± 11), (49 ± 9) kU/L] than those in group A [(78 ±8), (75 ±7), (79 ±6) kU/L](P＜ 0.05),and MDA and NO were lower at T1-T4 in group B and group C than that in group A(P＜ 0.05). SOD was higher at T3 in group C than that in group B (P ＜ 0.05), MDA at T1-T4 and NO at T2-T4 were lower in group C than those in group B (P ＜ 0.05). Conclusion HFJV can effectively decrease oxidative stress in patients during OLV.     

高频喷射通气对单肺通气患者氧化应激反应的影响

Objective To determine the effects of high-frequency jet ventilation (HFJV) on oxidative stress in patients during one-lung ventilation (OLV). Methods Forty-five patients undergoing elective radical esophageal cancer resection were divided into three groups with 15 cases each by random digits table: two lung ventilation group ( group A), OLV group (group B), HFJV- OLV group ( group C, working pressure 1 kg/cm2 and frequency 100 times/min). Venous blood samples were taken before induction (T0),at30min (T1),90min (T2),150min (T3) after OLV and the end of operation (T4) for measuring serum superoxide dismutase(SOD),malondialdehyde (MDA) and nitric oxide (NO). Results SOD was lower at T2-T4 in group B[(47 ± 10), (37 ±9), (41 ±7) kU/L] and group C[(58 ± 12), (51 ± 11), (49 ± 9) kU/L] than those in group A [(78 ±8), (75 ±7), (79 ±6) kU/L](P＜ 0.05),and MDA and NO were lower at T1-T4 in group B and group C than that in group A(P＜ 0.05). SOD was higher at T3 in group C than that in group B (P ＜ 0.05), MDA at T1-T4 and NO at T2-T4 were lower in group C than those in group B (P ＜ 0.05). Conclusion HFJV can effectively decrease oxidative stress in patients during OLV.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

低温与振动联合作用对周围循环功能及神经功能的影响

Objective To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.Methods 64 rabbits were divided into control group,low temperature group,vibration group and combined effect group randomly,16 each group.The changes of concentration of ET,Ang Ⅱ,NO in plasma and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were measured before and after experiment.Results After experiment,the concentration of ET,Ang Ⅱ,NO and SCV,amplitude of sensory nerve action potential,latency of sensory nerve action potential,MCV,distal amplitude of motor nerve,and distal latency of motor nerve were (68.84±14.81) pg/ml,(544.01±70.20) pg/ml,(123.73±9.58) nmol/ml,(25.36±6.96) m/s,(1.84±0.65) μV,(4.05±1.04) m/s,(27.40±6.05) m/s,(1.60±0.52) μV,(3.51±1.30) m/s respectively in low temperature group;(70.22±15.02) pg/ml,(540.77±68.25) pg/ml,(129.46±11.99) nmol/ml,(27.69±6.16) m/s,(2.19±0.53)μV,(3.86±0.89) m/s,(30.03±5.21) m/s,(1.65±0.49)μV,(3.36±1.11)m/s respectively in vibration group;(88.47±13.20) pg/ml,(687.38±101.44) pg/ml,(70.66±4.99) nmol/ml,(20.82±3.65)m/s,(1.21±0.64)μV,(5.05±0.94) m/s,(19.97±4.37) m/s,(1.09±0.49)μV,(4.49±1.26) m/s respectively in eombined effect group;compared with pre-experiment,the concentration of ET and Ang Ⅱ in low temperature group,vibration group and combined effect group were increased after experiment,and the NO was decreased (P＜0.05);the nerve conduct velocity and amplitude was decreased and the latency was delayed (P＜0.05).After experiment,the concentrations of ET and Ang Ⅱ in combined effect group were higher than low temperature group and vibration group,and the concentration of NO in combined effect group was lower than low temperature group and vibration group(P＜0.05).After experiment,the SCV and MCV in combined effect group were slower than low temperature group and vibration group;the amplitude of sensory nerve action potential and distal amplitude of moor nerve were less than low temperature group and vibration group;the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group.The factorial analysis results indicated the synergistic effect between low temperature and vibration(P＜0.05).Conclusion Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.     

还原型谷胱甘肽预处理对单肺通气患者围手术期炎性因子和自由基的影响

目的　探讨还原型谷胱甘肽(GSH)预处理对单肺通气患者围手术期炎性因子和自由基的影响.方法选择30例择期行肺叶切除术的肺癌患者,按随机数字表法分为GSH组和对照组,每组15例.GSH组于麻醉诱导后单肺通气前静脉输注GSH　30　mg/kg,对照组静脉输注等量0.9％氯化钠.于麻醉诱导前(T0)、单肺通气30　min(T1)、单肺通气60　min(T2)、恢复双肺通气60　min(T3)和术后2　h(T4)采集桡动脉血,测定血浆肿瘤坏死因子(TNF)-α、白细胞介素(IL)-8、丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性.结果T2～T4时两组MDA较T0时显著升高(P＜　0.05)[　GSH组T0～T4分别为(3.5±0.6)、(3.8±0.8)、(4.7±1.1)、(6.1±1.2)、(6.4±0.9)　mol/L;对照组T0～T4分别为(3.7±0.5)、(4.1±0.6)、(5.9±1.2)、(7.4±1.0)、(7.8±1.1)　mol/L],但GSH组显著低于对照组(P＜0.05).T2～T4时对照组SOD活性较T0时降低(P＜0.05),且低于GSH组(P＜0.05).T1～T4时两组TNF-α、IL-8较T0时显著升高(P＜0.05),但GSH组T2～T4时TNF-α及T1～T4时IL-8显著低于对照组(P＜0.05).结论　GSH　30　mg/kg预处理可减轻单肺通气患者肺的炎性反应及脂质过氧化程度.     

乙酰半胱氨酸对老年慢性阻塞性肺疾病患者炎性反应和氧化应激的影响

目的 探讨乙酰半胱氨酸对老年慢性阻塞性肺疾病(COPD)患者炎性反应和氧化应激的影响.方法 将60例COPD患者按机械抽样法随机分为治疗组和对照组,每组30例,对照组予以常规治疗,治疗组在此基础上加用乙酰半胱氨酸泡腾片治疗,疗程8周.两组患者在治疗前后均行血清肿瘤坏死因子α (TNF-α)、白细胞介素6(IL-6)、白细胞介素8(Ⅱ-8)、超氧化物歧化酶(SOD)和丙二醛(MDA)的检测.结果 治疗组治疗前后血清SOD、MDA分别为(51.08±7.80)、(75.09±8.03)μU/L和(7.13±0.89)、(4.51±0.61)μmol/L,对照组分别为(52.12±7.31)、(65.16±8.01)μU/L和(7.11±0.87)、(6.21±0.78)μmol/L,两组治疗后均较治疗前明显改善(P＜ 0.01或＜0.05),且两组治疗后比较差异亦有统计学意义(P＜0.05).治疗组治疗后血清IL- 6、IL-8、TNF-α均较治疗前下降[分别为( 18.25±7.24)ng/L比(29.02±6.70) ng/L、( 115.28±13.76) ng/L比(148.99±16.61)ng/L、(20.43±3.92) ng/L 比(32.32±8.18)ng/L](P＜0.05),对照组无明显改变[(25.25±7.55) ng/L比(28.82±7.54) ng/L、( 136.27±12.97) ng/L比(150.21±17.52) ng/L、(28.43±3.92) ng/L比(32.56±8.78) ng/L](P＞ 0.05);两组治疗后比较差异有统计学意义(P＜0.05).结论 乙酰半胱氨酸可改善COPD患者氧化/抗氧化失衡和减轻炎性反应,为COPD的治疗发挥作用.     

急性一氧化碳中毒患者心肌损伤程度与和肽素及缺血修饰白蛋白水平的相关性分析

目的 研究和肽素、缺血修饰白蛋白（IMA）与急性一氧化碳中毒（ACOP）心肌损伤的关系.方法 选择不同中毒程度ACOP患者110例作为中毒组,其中轻度中毒22例（轻度中毒组）,中度中毒50例（中度中毒组）,重度中毒38例（重度中毒组）,同期健康体检者30例作为对照组,中毒组在入院后2h、7d抽取静脉血3ml,对照组在体检时抽取静脉血3ml,检测心肌肌钙蛋白Ⅰ（cTnⅠ）、IMA、和肽素水平.中毒组依据有无出现并发症,分为并发症组26例,非并发症组84例.比较各组cTnⅠ、IMA、和肽素水平的差异.结果 入院后2h,轻度、中度、重度中毒组IMA降低[（62.50 ±2.17）、（59.04±3.10）、（56.01±8.85） kU/L]、和肽素升高[（2.82±0.73）、（7.31±0.95）、（13.08±1.96） μg/L],与对照组[（67.23±1.40） kU/L和（0.87±0.19）μg/L]比较差异有统计学意义（P＜0.05）,而且中度中毒组与轻度中毒组、重度中毒组与轻度中毒组和中度中毒组比较差异均有统计学意义（P＜0.05）;各组cTnⅠ比较差异无统计学意义（P＞0.05）.入院后7d,各组IMA、和肽素比较差异无统计学意义（P＞0.05）;轻度、中度、重度中毒组cTnⅠ升高[（1.80±0.17）、（2.34±0.46）、（2.60±0.54） μg/L],与对照组[（1.27±0.28）μg/L]比较差异有统计学意义（P＜0.05）,而且中度中毒组与轻度中毒组、重度中毒组与轻度中毒组和中度中毒组比较差异均有统计学意义（P＜0.05）.并发症组入院后2h和入院后7dIMA、和肽素水平均高于非并发症组[（54.62±1.53） kU/L比（57.89±4.02） kU/L、（60.65±3.61） kU/L比（66.84±1.78） kU/L和（13.88±1.45） μg/L比（6.99±3.39）μg/L、（6.65±1.82） μg/L比（2.47±0.61）μg/L],差异有统计学意义（P＜0.05）.并发症组入院后7 dcTnⅠ水平明显高于非并发症组[（3.10±0.22） μg/L比（1.87±0.27）μg/L],差异有统计学意义（P＜0.05）.相关分析提示不同程度的ACOP患者早期和肽素水平与IMA水平呈负相关（r=-0.560,P＜0.01）.结论 检测IMA和和肽素对ACOP心肌损伤早期诊断、判断预后转归及指导临床合理用药具有重要意义.     

短期胰岛素泵强化治疗对初诊2型糖尿病患者循环内皮祖细胞水平的影响及其抗氧化机制

目的 观察短期胰岛素泵(CSⅡ)强化治疗对初诊2型糖尿病(T2DM)患者外周血循环内皮祖细胞(EPCs)的保护作用及其抗氧化机制.方法 选取初诊T2DM患者40例(T2DM组),健康体检者30例(对照组),采用密度梯度离心法收集外周血单个核细胞,诱导分化培养7 d后,激光共聚焦显微镜鉴定FITC-UEA和DIL-acLDL双染色阳性细胞为正在分化的EPCs,并在倒置显微镜下计数.采用半自动生化仪测定血清活性氧活力(ROS)、丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD).结果 与对照组比较,T2DM组外周血EPCs减少[(46±3)个比(23±4)个](P<0.01),ROS、MDA增高[分别为(1125±687)U/ml比(2088±765)U/ml、(4.58±3.03)μmol/L比(8.32±4.15)μmol/L](P<0.01),GSH、SOD减低[分别为(263 ±35)mg/L比(206±41)mg/L、(103±7)U/ml比(80±15)U/ml](P<0.05).EPCs与ROS、MDA呈负相关(r=-0.7352,P<0.01;r=-0.8454,P<0.01),与GSH、SOD呈正相关(r=0.5435,P<0.05;r=0.6437,P<0.05).经短期CSⅡ强化治疗后T2DM患者EPCs明显增加(P<0.05),ROS、MDA明显降低(P<0.05),GSH、SOD明显增加(P<0.05).结论 短期CSⅡ强化治疗可明显减轻T2DM患者体内氧化应激,促进EPCs数量的恢复,进而防止血管病变.     

心肺复苏后患者血清抗脑抗体水平的变化及其意义

目的 研究心肺复苏后患者血清抗脑抗体水平变化及其意义.方法 将27例心脏骤停后心肺复苏成功并存活12周以上的患者根据第12周时格拉斯哥昏迷量表（GCS）评分,将其分为轻中型组12例（GCS评分≥8分）及重型组（GCS评分≤7分）15例.比较两组患者在自主循环恢复（ROSC）后第3,7天和第2,3,4,12周时的血清抗脑抗体水平,并与对照组（15例同期健康查体人群）进行比较.结果 轻、中型组与重型组患者血清抗脑抗体水平在ROSC后第3,7天和第2,3,4,12周时均显著高于对照组[（1.34±0.23）,（1.30 ±0.27） kU/L比（0.28±0.05）kU/L,（1.38±0.33）,（1.44±0.30） kU/L比（0.28±0.05） kU/L,（1.44±0.31）,（1.51±0.33） kU/L比（0.28±0.05） kU/L,（1.53±0.27）,（1.67±0.36） kU/L比（0.28±0.05） kU/L,（1.72±0.25）,（1.93±0.44） kU/L比（0.28±0.05） kU/L,（1.98±0.45）,（2.15±0.52） kU/L比（0.28±0.05） kU/L],差异有统计学意义（P＜0.01）;重型组患者血清抗脑抗体水平在各时间点均明显高于轻、中型组,差异有统计学意义（P＜0.05）.结论 心肺复苏后患者血清抗脑抗体水平明显升高;血清抗脑抗体水平对心肺复苏后患者的脑功能损伤程度和预后有判断价值.     

高浓度矽尘接触对大鼠氧化应激的作用研究

目的探讨大鼠高浓度矽尘接触过程中是否存在氧化应激反应。方法选40只SPF级Wistar雄性大鼠随机分为4组,即高剂量染尘组（1 000mg/m^3）、中剂量染尘组（500mg/m^3）、低剂量染尘组（100mg/m^3）和对照组,选用自然动式染尘装置每天染尘2h。染尘49d后处死大鼠,测定肺组织匀浆超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）活性及丙二醛（MDA）、还原性谷胱甘肽（GSH）含量。结果长时间、高浓度矽尘接触降低大鼠肺组织的SOD（30.25±0.49）U/ml、T-AOC活性（7.93±0.74）kU/L和GSH（2.34±0.96）g/L含量,同时MDA（5.65±0.13）nmol/ml水平升高,与对照组比较,差异有统计学意义（P〈0.01）。结论大鼠高浓度矽尘接触过程早期炎症反应发生可能与机体氧化应急有关。     

普罗布考对早期糖尿病视网膜病变患者氧化应激水平及黄斑水肿作用观察

目的 研究普罗布考对非增殖型糖尿病变视网膜病变（NPDR）患者血脂、血清总抗氧化能力（TAOC）、丙二醛（MDA）浓度、超氧化物歧化酶（SOD）水平及黄斑水肿的影响,为普罗布考防治早期DR提供临床依据.方法 纳入66例伴NPDR的2型糖尿病患者127眼,随机分为对照组和治疗组：对照组进行强化降血糖、血压治疗,治疗组在强化治疗基础上加用普罗布考0.375 g,2次/d,口服,总疗程为12月.治疗前后两组患者均进行了血脂、氧化应激指标及眼底荧光血管造影检查.结果 共有62例120眼完成研究,普罗布考组患者总胆固醇（TC）[（3.6±0.58）mmol/L VS（4.71±0.61）mmol/L,t=7.65,P＜0.01]、甘油三酯（TG）[（1.07±0.35）mmol/L VS（1.23±0.43）mmol/L,t=2.02,P＜0.05]、低密度脂蛋白胆固醇（LDLC）[（2.0±0.47）mmol/LVS（2.55±0.56）mmol/L,t=4.18,P＜0.01]及丙二醛（MDA）[（10.35±2.97）nmol/L VS（14.83±2.75）nmol/L,t=6.18,P＜0.01]水平降低,患者总抗氧化能力（TAOC）[（19.25±4.11）u/ml VS（16.63±3.27）u/ml,t=3.57,P＜0.01]及超氧化物歧化酶（SOD）[（94.52±10.28）u/ml VS（75.37±9.87）u/ml,t=8.62,P＜0.01]水平显著提高,且患者眼底黄斑水肿有明显减轻（Х^2=4.219,P＜0.05）.结论 普罗布考对于NDPR患者除了降脂作用外,还具有明显的抗氧化作用,显著降低患者黄斑水肿发生率,提示普罗布考对NDPR患者具有一定的治疗作用.     

低温与振动联合作用对周围循环功能及神经功能的影响

目的 探讨低温与振动联合作用对家兔周围循环功能与神经功能的影响.方法 将64只家兔随机分为对照组、低温组、接振组以及联合作用组,每组16只.试验前后测定血浆中内皮素(ET)、血管紧张素Ⅱ(Ang Ⅱ)、一氧化氮(NO)浓度以及感觉神经传导速度(SCV)、感觉神经动作电位波幅、感觉神经动作电位潜伏时、运动神经传导速度(MCV)、运动神经远端波幅、运动神经远端潜伏时的变化.结果 试验后低温组ET浓度、AngⅡ浓度、NO浓度以及SCV、动作电位波幅、动作电位潜伏时,MCV、远端波幅、远端潜伏时分别为(68.84±14.81)pg/ml、(544.01±70.20)pg/ml、(123.73±9.5s)nmol/ml、(25.36±6.96)m/s、(1.84±0.65)μV、(4.05±1.04)m/s、(27.40±6.05)m/s、(1.60±0.52)μV、(3.51±1.30)m/s;接振组分别为(70.22±15.02)pg/ml、(540.77±68.25)pg/ml、(129.46±11.99)nmol/ml、(27.69±6.16)m/s、(2.19±0.53)μV、(3.86±0.89)m/s、(30.03±5.21)m/s、(1.65±0.49)μV、(3.36±1.11)m/s;联合作用组分别为(88.47±13.20)pg/ml、(687.38±101.44)pg/ml、(70.66+4.99)nmol/ml、(20.82±3.65)m/s、(1.21±0.64)μV、(5.05±0.94)m/s、(19.97±4.37)m/s、(1.09±0.49)μV、(4.49±1.26)m/s.与试验前比较,试验后各试验组ET、AngⅡ浓度升高,NO浓度降低;神经传导速度减慢,波幅降低,潜伏时延长,差异均有统计学意义(P＜0.05).试验后,联合作用组ET、Ang Ⅱ浓度高于低温组和接振组,NO浓度低于低温组和接振组;联合作用组SCV、MCV慢于低温组和接振组,感觉神经动作电位潜伏时、运动神经远端波幅低于低温组和接振组,感觉神经动作电位潜伏时、运动神经远端潜伏时长于低温组和接振组,差异均有统计学意义(P＜0.05).对各指标进行析因分析,结果显示,低温与振动间存在协同作用(P＜0.05).结论 低温能够加剧振动性周围循环功能与神经功能损伤.     

氧化应激在间歇低氧诱导大鼠海马损伤中作用的研究

目的 探讨氧化应激在间歇低氧（IH）诱导大鼠海马损伤中的作用.方法 30只成年雄性Sprague-Dawley 大鼠随机分成对照组（CON组）、间歇低氧组（IH组）、Melatonin组（MEL组）,每组10只.采用化学比色法测定大鼠海马组织丙二醛（MDA）、超氧化物歧化酶（SOD）水平,并采取RT-PCR方法检测海马组织中Cu/Zn SOD、GPx、CAT的mRNA水平.结果 （1）在IH组中,MDA的水平为（1.68±0.23）μmol/g protein,均高于对照组和MEL组的（1.25±0.14）μmol/g protein和（1.35±0.18）μmol/g protein（P＜0.05或P＜0.01）;（2）在IH组中,SOD的活性、Cu/ZnSOD、GPx、CAT的mRNA表达水平分别为（43.01±4.96）103NU/g protein、0.25±0.02、0.34±0.09、0.38±0.03,均低于对照组和MEL组的（61.12±5.68）103NU/g protein和（55.98±4.65）103NU/g protein、0.48±0.06和0.43±0.08、0.55±0.07和0.54±0.05、0.57±0.04和0.53±0.07（P＜0.05,P＜0.01）.结论 间歇低氧可通过氧化应激导致大鼠海马损伤,MEL能抑制间歇缺氧导致的氧化应激,从而对间歇低氧大鼠海马损伤具有保护作用.