不同配体对Fe(Ⅱ)及Fe(Ⅲ)所诱导的脂质过氧化的影响

本文以TBA法研究了在有和无抗坏血酸存在时,10种阴离子对Fe(Ⅱ)所诱导的6种多糖—Fe(Ⅲ)配合物和四种核苷酸—Fe(Ⅲ)配合物对卵磷脂脂质过氧化的影响。结果表明,D—葡萄糖醛酸根对Fe(Ⅱ)和碱解银耳多糖—Fe(Ⅲ)以及AMP—Fe(Ⅲ)配合物所诱导的过氧化程度,不论有或无抗坏血酸存在均较小,可供临床参考。     

壳聚糖/羧甲基壳聚糖金属配合物对氧自由基的清除作用研究

以NADH-PMS-NBT体系产生超氧阴离子自由基(o2-·)和EDTANa2·Fe(Ⅱ)-H2O2体系产生 羟自由基(·OH)来研究壳聚糖Cu(Ⅱ)、Zn(Ⅱ)配合物、羧甲基壳聚糖Cu(Ⅱ)、Zn(Ⅱ)配合物时氧自由基的 清除作用。结果显示配合物对O2-·和·OH均具有明显的清除作用,其中铜(Ⅱ)配合物对O2-·的清除活 性最高,而锌(Ⅱ)配合物比铜(Ⅱ)配合物具有更强的清除·OH的能力,羧甲基壳聚糖Cu(Ⅱ)、Zn(Ⅱ)配合 物与含有相同金属离子的壳聚糖配合物相比对O2-·和·OH具有更高的抑制活性。     

芦荟大黄素金属配合物的抗氧化活性研究

目的 合成3种芦荟大黄素金属配合物,并比较配体和配合物的抗氧化活性.方法 在无水乙醇溶剂中,通过溶解、搅拌合成芦荟大黄素-铁(Ⅱ)、芦荟大黄素-铜(Ⅱ)、芦荟大黄素-镁(Ⅱ)3种配合物,并对其结构进行表征,采用Fe2+-H2O2-亚甲蓝法、二苯代苦味肼基自由基法(DPPH法)等检测配体和配合物对超氧自由基(O2-?)、...     

甘氨酸、DL-丙氨酸与Cu(Ⅱ)、Ni(Ⅱ)、Co(Ⅱ)、Zn(Ⅱ)三元配合物稳定性的研究

在37±0.1℃、离子强度0.15mol/L(KNO_3)条件下,作者用pH电位法研究了甘氨酸、DL-丙氨酸与Cu(Ⅱ)、Ni(Ⅱ)、Co(Ⅱ)、Zn(Ⅱ)所形成的二元及三元配合物,并分别测定了在上述条件下甘氨酸、DL-丙氨酸的离解常数及与这些金属离子所形成的二元及三元配合物的稳定常数,同时绘出了三元体系中各配合物的物种随pH值改变的分布图。     

α,α''''-联吡啶Fe(Ⅱ)光纤探针的研究

光导纤维化学传感器是一种灵敏度高、可连续自动遥测的新型微量和痕量分析技术 ,是近十几年来分析化学的一项重大发展[1— 5] 。α ,α 联吡啶是一种性能较优的测铁光度试剂[6 ] 。本文利用α ,α 联吡啶在PVF膜上吸附的牢固性以及与Fe(Ⅱ )络合物的高度稳定性 ,将Fe(Ⅱ )在膜上富集并显色 ,然后在光纤光度计上测定膜上络合物的吸光度 ,本法可测定 0 0 5— 1 0ppm范围内的Fe(Ⅱ )。     

糊精—柠檬酸—Fe(Ⅲ)配合物的合成及其性能研究

实验研究了糊精(或糊精水解物)-柠檬酸-Fe(Ⅲ)三元配合物的合成条件及某些化学性能。证明Fe(Ⅲ)与糊精水解产物的配合能力较糊精为强。红外光谱显示Fe(Ⅲ)与糊精和糊精水解产物所形成的配合物具有相同的配位结构。水溶液都不显示游离Fe(Ⅲ)离子的特效反应,在pH值达11时均未出现沉淀,说明该类化合物比较稳定。还原动力学实验证明该类配合物中Fe(Ⅲ)易被还原。     

分光光度法测定琥珀酸亚铁片中铁的含量

目的建立分光光度法测定琥珀酸亚铁片中铁的含量的方法。方法在酸性条件下,Fe2+与邻二氮菲生成有色配合物,该配合物在510 nm处有最大吸收,利用分光光度法测定铁的含量。结果Fe2+的线性范围为0.042 44~2.546 4 mg.L-1,方法平均回收率为99.5%,日内及日间精密度相对标准偏差(RSD)小于1.9%。结论用分光光度法测定琥珀酸亚铁片含量简便、快速、准确,并可同时测定琥珀酸亚铁片中Fe2+和Fe3+的含量,可用于该制剂的质量控制。     

HPMTP缩L-苯丙氨酸乙酯席夫碱配体及其配合物的合成、结构表征和抑菌活性

目的合成1-苯基-3-甲基-4-甲基苯甲酰基-5-吡唑啉酮(HPMTP)缩L-苯丙氨酸乙酯席夫碱及其Cu(Ⅱ)、Co(Ⅱ)、Ni(Ⅱ)、Fe(Ⅱ)、Mn(Ⅱ)金属配合物,并对合成的化合物进行结构表征和抑菌活性检测。方法通过元素分析、核磁(1H NMR)和红外光谱(IR)对合成化合物进行结构表征检测,采用琼脂扩散法测定化合物对大肠杆菌(革兰阴性菌)和枯草芽孢杆菌(革兰阳性菌)的抑菌活性。结果由1H NMR和IR数据推断出席夫碱配体及其金属配合物可能的结构;发现在1.25、2.50、5.00 g·L-1时,HPMTP、HPMTP缩L-苯丙氨酸乙酯席夫碱及其金属配合物对革兰阳性/阴性菌都具有抑制活性,其中浓度为2.50 g·L-1时抑菌效果最佳。抑菌能力的强弱是金属配合物>席夫碱配体>HPMTP,其中Cu(Ⅱ)和Co(Ⅱ)配合物的活性最高。结论 Cu(Ⅱ)和Co(Ⅱ)配合物具有进一步研究开发为广谱抗菌药物的潜力。     

Fe(Ⅱ)-MTB-CPB分光光度法测定Fe(Ⅱ)

Fe（Ⅱ）与甲基百里酚蓝（MTB）、溴化十六烷基吡啶（CPB）可发生显色反应，该三元配合体系至少稳定10h。在pH4.7时有最大吸收，最大吸收波长为530nm；摩尔吸光系数为2.79×104L／（mol·cm）。Fe（Ⅱ）含量在0～35μg／25ml时符合比尔定律。配合物的组成为Fe（Ⅱ）：MTB：CPB＝1：2：2。在有大量EGTA和NH4F存在的情况下，三元配合体系不被破坏，因而可以用来选择性地测定Fe（Ⅱ）的含量。     

酮类Schiff碱的合成与荧光性质研究

目的:研究所合成的酮类Schiff配体及其配合物的荧光性质。方法:合成了一种酮类双Schiff碱配体及其过渡金属Cu(Ⅱ)、Zn(Ⅱ)的Schiff配合物,并用元素分析、红外光谱、电子光谱、摩尔电导、1 H NMR对其结构进行了表征,测定了所合成的Schiff配体及其配合物的荧光光谱。结果:室温下,测得配体与Zn(OAc)2合成的配合物的固体荧光光谱和在不同溶剂中的荧光光谱。其在紫外光的照射下会发出黄绿色荧光。配合物L.Zn(OAc)2激发峰在468nm;荧光峰在538nm,相对强度(RFI):776.6。配体与Cu(Ⅱ)形成的配合物几乎观察不到荧光。结论:L.Zn(OAc)2配合物有良好的荧光性能,且在固体状态下的荧光远远强于在溶液中的荧光。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.