腹腔镜手术治疗Mirizzi综合征分析

目的探讨腹腔镜下Mirizzi综合征（Ms）手术适应证及手术方法。方法回顾性分析腹腔镜下治疗的14例Mirizzi综合征的手术方法和疗效。结果14例全部采用腹腔镜手术治疗,手术方式包括胆囊切除术7例,胆囊大部分切除术3例,胆囊切除或胆囊部分切除直接修补瘘口放置T管4例。腹腔镜术后14例均获随访,随访时间为1．3年,无明显手术并发症。结论Mirizzi综合征的术前和术中诊断及分型直接关系到手术适应症及手术方法的选择。在熟练掌握腹腔镜技术的前提下腹腔镜治疗Mirizzi综合征的Ⅰ型和Ⅱ型是安全可行的方法。     

Mirizzi综合征的腹腔镜治疗

目的:探讨腹腔镜胆囊切除术对Mirizzi综合征的效果及术中处理方法.方法:对采用LC治疗的16例Mirizzi综合征病例进行回顾性研究.结果:16例采用腹腔镜手术治疗,Ⅰ型12例行胆囊切除术,Ⅱ型2例行胆囊大部切除术,Ⅲ型1例行T管引流术,Ⅳ型1例行胆肠Roux-en-Y吻合.结论:在熟练掌握腹腔镜的前提下,腹腔镜治疗Mirizzi综合征的Ⅰ型、Ⅱ型是安全可行的方法.     

Mirizzi综合征的诊断和腹腔镜胆囊切除术14例临床体会

目的探讨Mirizzi综合征的诊断和腹腔镜胆囊切除术（LC）术中处理方法。方法回顾性分析2002年1月～2009年2月965例LC中14例Mirizzi综合征患者的临床资料。结果18例中Ⅰ型12例,11例施行LC术,1例中转开腹;Ⅰ型4例,3例施行LC术,行胆囊大部分切除术、瘘口修补术、胆总管切开T管引流术,1例中转开腹;Ⅲ型2例均中转开腹,所有病例均痊愈。无胆瘘病例,无胆管狭窄并发症发生。结论B超是首选的检查方法．ERCP、MRCP能提高术前诊断率,腹腔镜能安全处理大部分Ⅰ型Mirizzi综合征和部分Ⅱ型Mirizzi综合征病例。     

Mirizzi 综合征的腹腔镜治疗体会

[目的] 探讨腹腔镜胆囊切除术中Mirizzi综合征的治疗方法.[方法] 回顾分析3517例腹腔镜胆囊切除术中17例Mirizzi综合征的临床资料.[结果] 本组17例患者中15例在腹腔镜下完成,行胆囊全切除或大部切除或加T管引流术,其中2例手术困难中转开腹手术.所有患者均获长期随访,无严重的并发症发生.[结论] 腹腔镜胆囊切除术是治疗Mirizzi综合征Ⅰ、Ⅱ型安全有效的方法.     

Mirizzi综合征的腹腔镜治疗

目的探讨腹腔镜治疗Mirizzi综合征的手术方法及疗效。方法回顾分析1993年1月～2007年12月98例Mirizzi综合征的临床资料,对其手术方式进行总结。结果按Csendes分型,Ⅰ型62例,Ⅱ型25例,Ⅲ型8例,Ⅳ型3例。所有患者均作了胆囊切除、瘘口修补或胆囊大部切除、胆管修复、T管引流术。术中胆管损伤1例,术后漏胆1例。结论在腹腔镜下采取不同手术方式和耐心细致的操作可顺利处理Mirizzi综合征。     

Mirizzi综合征20例诊治体会

目的：探讨Mirizzi综合征的诊断和治疗特点。方法：对20例经手术证实的Mirizzi综合征进行回顾性分析。结果：20例中Ⅰ型15例,Ⅱ型2例,Ⅲ型3例,无Ⅳ型病例。行胆囊切除术11例,胆囊大部分切除术4例,胆囊部分切除加瘘口修补术1例,胆囊造痿术1例,胆总管空肠Roux—en—y吻合术3例。手术效果满意17例。并发胆瘘3例。结论：B型超声是术前常规检查,ERCP、MRCP可以提高术前确诊率。手术方式应根据病理分型来决定。     

Mirizzi综合征8例临床诊治体会

目的 总结Mirizzi综合征的诊断和治疗经验,探讨其临床特点.方法 对我院2001～2008年在胆囊手术中发现并经手术证实的8例Mirizzi综合征的临床资料进行回顾性分析和总结.结果 8例Mirizzi综合征患者中,术前经ERCP及B超检查明确诊断者仅1例,其余7例均为术中诊断.Ⅰ型5例,行单纯胆囊切除术;Ⅱ型3例,其中行胆囊切除+胆总管探查修补T管引流术2例,胆囊大部切除加胆囊瓣瘘口修补术1例.结论 Mirizzi综合征术前诊断较困难,治疗应根据不同类型选择适当的手术方式.     

腹腔镜在Mirizzi综合征的应用

目的 探讨腹腔镜手术在Mirizzi综合征中诊治的价值.方法 回顾性分析我院1992～2007年腹腔镜胆囊切除术(LC)诊治47例Mirizzi综合征的临床资料.结果 成功完成腹腔镜胆囊手术(LC)36例,其中Ⅰ型28例,Ⅱ型8例,中转手术11例,其中肝胆管损伤3例(Ⅱ型2例,Ⅲ型1例).结论 腹腔镜对Mirizzi综合征(Ⅰ型,大部分Ⅱ型)行胆囊切除是安全可行的,Ⅲ、Ⅳ型应及时中转开腹完成手术.     

Mirizzi综合征临床诊治分析

目的:探讨Mirizzi综合征的诊断和治疗方法,提高其诊断和治疗水平。方法:回顾分析32例经手术证实的Mirizzi综合征诊断和手术治疗情况。结果:32例Mirizzi综合征病人均为术中确诊。按Csends分型,Ⅰ型20例,Ⅱ型9例,Ⅲ型3例,未发现Ⅳ型。32例行胆囊切除、瘘口修补T管引流或胆肠Roux-en-y吻合术。胆漏2例。结论:Mirizzi综合征术前诊断困难,术中明确诊断及妥善处理是防止并发症的主要措施。     

Mirizzi综合征36例临床诊断与治疗体会

目的：探讨Mirizzi综合症的诊断治疗特点,以提高其诊断和治疗水平。方法：对36例经手术证实为Mirizzi综合症的临床资料进行回顾性分析。结果：36例Mirizzi综合症患者中仅4例（11.1%）术前确诊。按Csendes分型,Ⅰ型7例,Ⅱ型18例,Ⅲ型9例,Ⅳ型2例。5例行胆囊切除术,2例行胆囊大部分切除术;胆囊切除、瘘口修补10例,13例行胆囊切除、胆总管探查、瘘口修补、T管引流术;胆囊切除、胆肠吻合术5例。术后恢复好,随访无严重并发症。结论：Mirizzi综合症术前确诊困难,B超结合MRCP/ERCP检查可以提高Mirizzi综合症的术前确诊率,手术容易损伤胆管,手术方式应据病理损伤程度决定。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.