球形芽孢杆菌C_（3－41）块剂和颗粒剂防制城市致乏库蚊动虫效果评价

本文报道球形芽孢杆菌Ｃ３－４１（下称Ｂ。ｓＣ３－４１）乳剂、颗粒剂和块剂控制致乏库蚊幼虫的效果及其评价。结果表明：乳剂和颗粒剂的即杀效果比块剂好，２００ＩＴＵ／μｌ的Ｂ．ｓＣ３－４１乳剂１、２、３ｍｌ／ｍ２和１４０ＩＴＵ／μｌ的颗粒剂４．８ｇ／ｍ２，４８ｈ平均幼虫下降率．分别为８２～９６％和８８￣１００％；而１６０ＩＴＵ／μｌ的块剂４．８ｇ／ｍ２，４８ｈ平均幼虫下降率只有３３～５６％。３种剂型的持效以块剂为佳，可达３个月以上。     

野外致倦库蚊对球形芽孢杆菌C3-41的抗性

目的：探讨野外致倦库蚊对球形芽孢杆菌Ｃ３－４１的抗性。方法：为ＷＨＯ常规生物测定方法。结果：经连续８年的生物防制,野外致倦库蚊幼虫对球形芽孢杆菌Ｃ３－４１的抗性水平高达２４５４５倍,但对球形芽孢杆菌ＬＰ１－Ｇ未表现出抗性,而对苏云金杆菌以色列亚种（Ｂ．ｔｈｕｒｉｎｇｉｅｎｓｉｓｉｓｒａｅｌｅｎｓｉｓ）的敏感性却提高１倍。结论：可选用后两种生物制剂进行抗性蚊幼虫的防制     

球形芽孢杆菌C_（3－41）对嗜人按蚊和东乡伊蚊幼虫的毒性测定

本文报道球形芽孢杆菌Ｃ３－４１对嗜人按蚊和东乡伊蚊的毒性。实验室生物测定结果表明，ＢｓＣ３－４１液剂对嗜人按蚊Ⅳ龄幼虫处理后２４ｈ和４８ｈ的ＬＣ５分别为２．４０ｐｐｍ和１．７８ｐｐｍ，对东乡伊蚊的ＬＣ５０分别为５．３５ｐｐｍ和３．１４ｐｐｍ；持效为１～２周。Ｂｓ．Ｃ３－４１粉剂对嗜人按蚊和东乡伊蚊处理后２４ｈ的ＬＣ５０分别为３．３３ｐｐｍ和４．４４ｐｐｍ，处理后４８ｈ的ＬＣ５０分别为１．３６ｐｐｍ和１．４４ｐｐｍ。球形芽孢杆菌Ｃ３－４１处理蚊幼虫４８ｈ后的效果明显优于处理后２４ｈ的。     

球形芽孢杆菌C3—41对嗜人按蚊和东乡伊蚊幼虫的毒性测定

本文报道球形芽孢杆菌Ｃ３－４１对嗜人按蚊和东乡伊蚊的毒性，实验室生物测定结果表明，ＢｓＣ３－４１液剂对嗜人按蚊Ⅳ龄幼虫处理后２４ｈ和４８ｈ的ＬＣ５０分别为２．４０ｐｐｍ和１．７８ｐｐｍ，对东乡伊蚊的ＬＣ５０分别为５．３５ｐｐｍ和３．１４ｐｐｍ，持效为１～２周，Ｂｓ，Ｃ３－４１粉剂对嗜人按蚊和东乡伊蚊处理后２４ｈ的ＬＣ５０分别为３．３３ｐｐｍ和４．４４ｐｐｍ，处理后４８ｈ的ＬＣ５０分别为１．３６ｐｐ     

球形芽孢杆菌发泡块剂灭致乏库蚊幼虫的效果

作者应用球形芽孢杆菌Ｂｓ－９４１发泡块剂（由本所生产）和Ｂｓ－Ｆｔ发泡块剂（德国提供），在实验室和现场进行灭致乏库蚊的效果比较。室内生物测定结果显示：Ｂｓ－９４１发泡块剂ＬＤ_（６０）为０．０１９３μｇ／ｍｌ，Ｂｓ－Ｆｔ发泡块剂ＬＤ_（６０）为０．００７μｇ／ｍｌ．在３．６～４．０ｍ￣２的现孳生地水池中，两种发泡剂使用剂量为０．５，１．０和１．５ｇ／ｍ￣２，制剂４８ｈ的灭蚊效果均可达１００％，当剂量为０．５ｇ／ｍ￣２时，Ｂｓ－９４１发泡块剂持效达１７天，而Ｂｓ－Ｆｔ发泡块剂只有９天；当剂量上升到１．５ｇ／ｍ￣２时，两种发泡剂的持效均可达到２１天。对照组乳剂Ｂｓ－Ｃ_（３ｃ－４１）的浓度为０．９２μｌ／ｌ（与１．５ｇ／ｍ￣２相当）时，持效为９天，说明本所生产的Ｂｓ发泡块剂在中小型挛生地中比Ｂｓ乳剂持效长，并且超过德国Ｂｓ发泡块剂的效果和持效。     

苏云金杆菌和球形芽孢杆菌（3—4）灭蚊幼漂浮颗粒剂的效果观察

湖北省寄生虫病防治研究所研制的Ｂｔｉ和ＢＳ－Ｃ３－４１漂浮颗粒剂在现场中对中华按蚊和致乏库蚊幼虫的效果进行了试验，结果显示Ｂｔｉ漂浮颗粒剂对稻田中的中华按蚊有较好的毒杀效果。剂量为０．４５ｇ／ｍ＾２时，２４小时中华按蚊幼虫密度下降率为８６。６％￣１００％。持效达１４￣１５天，ＢＳ－Ｃ３－４１漂浮颗粒剂剂量为０．４５ｇ／ｍ＾２￣０．５４ｇ／ｍ＾２，４８小时加幼虫密度下降率为９０．４％￣９７．４％，持     

苏云金杆菌以色列变种漂浮块剂现场灭致乏库蚊效果

应用湖北省医学科学院寄生虫病防治研究所研制的苏云金杆菌以色列变种（Ｂｔｉ）漂浮块剂在现场进行了灭致乏库蚊的效果评价，室内生物测定结果表明；Ｂｔｉ漂浮块剂对致加蚊ＬＤ５０为０．５２０７μｇ／ｍｌ，效价为２３３Ｉ／ｍｌ，在０．４－０．８ｍ＾２现场水缸中，用１０ｇ／ｍ＾２的剂量，第一次投药持１０天出现２龄幼虫，第二次投药特效可保持１１天，因此每１０天投一次药可有效控制水缸中蚊幼虫孳生。     

几种改进的漂浮颗粒剂灭中华按蚊和致倦库蚊的效果

目的：比较几种改进的漂浮颗粒剂灭中华按蚊和致倦库蚊幼虫的效果。方法：现场试验。结果：室内生物测定,Ｂ．ｔ．ｓ－１８７漂浮颗粒剂（荷叶型）ＬＤ５０为０．４０８７μｇ／ｍｌ,Ｂａｃｔｉｍｏｓ颗粒剂（荷叶型）ＬＤ５０为０．９３４４μｇ／ｍｌ,Ｂ．ｓ－Ｃ３－４１漂浮颗粒剂（荷叶型）ＬＤ５０为０．０５９９μｇ／ｍｌ。稻田灭中华按蚊幼虫的效果显示：两种Ｂ．ｔ．ｓ漂浮颗粒剂（荷叶型）０．８１ｇ／ｍ２剂量时,２４ｈ灭蚊效果均可达１００％。１８７漂浮颗粒剂持效可达１１ｄ,Ｂａｃｔｉｍｏｓ漂浮颗粒剂持效为８ｄ。１８７颗粒剂和Ｂ．ｓ－Ｃ３－４１颗粒剂现场灭致倦库蚊的效果显示：在０．６８ｇ／ｍ２剂量时,２４ｈ蚊幼密度下降率分别为１００％和９９．１％,持效分别为９ｄ和８ｄ。结论：采用荷叶粉为主要载体,在自然界中易于分解,不造成污染,而且灭蚊即效和持效均较好。     

球形芽孢杆菌C_（3－41）菌株杀蚊毒蛋白的生物合成

本文报道球形芽孢杆菌Ｃ３－４１菌株毒蛋白的生物合成的检测结果。对Ｃ３－４１菌株不同发育阶段发酵液的毒蛋白量进行了酶联免疫吸附测定（ＥＬＩＳＡ），并用生物测定方法检测了发酵液杀蚊毒性，证明其毒蛋白合成量与该菌芽孢的发育和形成密切相关，同时揭示了ＥＬＩＳＡ测出的毒蛋白量与生物测定所示的杀蚊毒力具有很高的符合率。     

基因工程灭蚊幼蓝藻的现场初步观察

将转入球形芽孢杆菌毒蛋白基因表达质粒的工程藻Ａｎａｂａｅｎａｓｕｂｔｒｏｐｉｃ（ｐＤＣ２６）大量培养后，应用于山东省平阴县进行现场观察。结果表明：工程藻在９．４１×１０５ｃｅｌｌｓ／ｍｌ浓度以上时，４８ｈ可杀死９９～１００％蚊幼虫；在９．１３×１０４ｃｅｌｌｓ／ｍｌ浓度时，４ｄ可杀死９９％以上蚊幼虫，连续观察２个多月，工程藻仍有明显杀蚊效果，实验组最多１４条／勺幼虫，而对照组２１０条／勺左右。观察还发现，工程藻对库蚊幼虫杀灭效果最好，其次为伊蚊，但高浓度时，对伊蚊仍有明显杀灭效果；由于按蚊幼虫密度太低，未能观察。此结果比单独使用球形芽孢杆菌杀灭蚊幼虫有明显提高，持续时间也延长，这将为蚊虫的持续控制提供一个新的有力武器。     

Synthesis, characterization, activities, cell uptake and DNA binding of trinuclear complex: [{trans-PtCl(NH(3))}(2)mu-{trans-Pt(NH(3))(2-hydroxypyridine)-(H(2)N(CH(2))(6)NH(2))(2)]Cl(4)

The trinuclear complex: [{trans-PtCl(NH(3))}(2)mu-{trans-Pt(NH(3))(2-hydroxypyridine)-(H(2)N(CH(2))(6)NH(2))(2)]Cl(4) (code named CH9) has been synthesized and characterized. The activity of the compound against human ovarian cancer cell lines: A2780, A2780(cisR) and A2780(ZD0473R), cell up take, level of binding with DNA and nature of its interaction with pBR322 plasmid DNA have been determined. Although the compound is found to be less active (about a half time as active as cisplatin) against the parent ovary cell line A2780, it is found to be more active than cisplatin against resistant cell lines: A2780(cisR) (3.6 times more) and A2780(ZD0473R) (3.4 times more). The higher activity of CH9 against the resistant cell lines suggests that the compound has been able to overcome multiple mechanisms of resistance operating in A2780(cisR) and A2780(ZD0473R) cell lines. Like other multicentered complexes, the compound is believed to form a range of interstrand GG adducts with duplex DNA that induces permanent global changes in the DNA conformation. This binding is different from that of cisplatin and ZD0473 that form mainly intrastrand adducts, inducing a local kink in a DNA strand. Increasing prevention of BamH1 digestion of form I and form II pBR322 plasmid DNA with the increase in concentration of CH9 provides support to the idea that global changes in DNA conformation are induced as a result of its interaction with the compound.     

Diagnostic image (3). (Lithopedion)

In a 62-year-old woman with aspecific symptoms in the lower abdomen a lithopaedion was diagnosed mimicking an ovarian tumour.     

Association of hexachlorobenzene (HCB), dichlorodiphenyltrichloroethane (DDT), and dichlorodiphenyldichloroethylene (DDE) with in vitro fertilization (IVF) outcomes

Background: Hexachlorobenzene (HCB), dichlorodiphenyltrichloroethane (DDT), and dichlorodiphenyldichloroethylene (DDE) are persistent chlorinated pesticides with endocrine activity that may adversely affect the early stages of human reproduction.Objective: Our goal was to determine the association of serum levels of HCB, DDT, and DDE with implantation failure, chemical pregnancy, and spontaneous abortion in women undergoing in vitro fertilization (IVF) from 1994 to 2003.Methods: Levels of HCB and congeners of DDT and DDE were measured in serum collected during the follicular phase. Multivariable-adjusted statistical models accommodating multiple outcomes and multiple cycles per woman were used to estimate the relation between serum pesticide levels and IVF outcomes.Results: A total of 720 women with a mean ± SD age 35.4 ± 4.2 years at enrollment contributed 774 IVF cycles. All samples had detectable levels of HCB, DDT, and DDE, with median levels of 0.087 ng/g serum for HCB, 1.12 ng/g serum for total DDT, and 1.04 ng/g serum for p,p´-DDE. Compared with the lowest quartile (Q1) of HCB, the lipid- and multivariable-adjusted odds ratio (OR) for failed implantation was significantly elevated for those with higher HCB quartiles [Q2–Q4; adjusted ORs: for Q2, 1.71; 95% confidence interval (CI): 1.03, 2.82; for Q3, 2.30; 95% CI: 1.39, 3.81; for Q4, 2.32; 95% CI: 1.38, 3.90] and showed a significantly increasing trend (p = 0.001). No statistically significant associations were observed between DDT/DDE and IVF outcomes or between HCB and chemical pregnancy or spontaneous abortion.Conclusions: Serum HCB concentrations were on average lower than that of the general U.S. population and associated with failed implantation among women undergoing IVF.     

水中汞离子(Ⅱ)的纳米生物传感器检测法

目的 运用汞离子特异性寡核苷酸探针和纳米金,构建一种能快速比色检测水中Hg2+的纳米生物传感器.方法 对已有的汞离子特异性核苷酸探针进行优化设计,并吸附到纳米金颗粒表面,实现生物传感器的组装.利用汞离子特异性稳定T-T错配和纳米金颗粒在高盐条件下的颜色变化,从而实现对Hg2+的定量分析.结果 制备的纳米金粒径为15 nm,浓度为2.97 nmol/L;成功获得2条截短的汞离子特异性核苷酸探针(9 bp);构建的汞离子纳米生物传感器,最佳盐浓度为0.5 mol/L,检测Hg2+仅需数分钟,检出限为10 μmol/L,特异性为100％.结论 本研究构建的纳米生物传感器,不需要标记探针或者修饰纳米金,在不使用任何特殊条件下就可以实现对汞离子可视化便捷分析,具有操作简便、检测时间短、灵敏度较高、特异性强等优点,易于推广使用.     

Survey of ethylenethiourea (ETU) in ethylenebis(dithiocarbamate) (EBDC) fungicides

Ethylenethiourea (ETU) has been recognized as a compound with adverse toxicological properties. It may occur in ethylenebis(dithiocarbamate) (EBDC) fungicides as a by-product and/or a degradation product. Restrictions have been issued by the Italian Health Authority to keep the level of ETU in EBDC formulations under 0.5% (on an EBDC technical product basis). A survey was carried out on 85 samples of commercial formulations from the Italian market to verify compliance with the above limitation. ETU was extracted by methanol and determined by gas chromatography with a flame ionization detector. Three of the 85 samples (3.5%) exceeded the limit of 0.5% established by the Italian legislation for ETU.     

Diagnostic image (5) (Ileum pathology)

In a 28-year-old woman Crohn's disease was diagnosed. She had drug treatment but the situation deteriorated and ileocoecal resection was carried out. In the surgical specimen the diagnosis was confirmed.     

非有机溶剂高聚物萃取法分离Cd~(2 )、Co~(2 )等多种离子混合液中Cd~(2 )的研究

〔目的〕Cd2 、Co2 、Cu2 、Fe3 、Ni2 混合溶液中Cd2 的分离。〔方法〕在HAc-NaAc缓冲溶液 ,聚乙二醇— 5 -Br-PADAP -硫酸铵体系中 ,水溶性高聚物萃取Cd2 、Co2 、Cu2 、Fe3 、Ni2 的行为研究。〔结果〕在pH =4.5时 ,Co2 、Cu2 、Fe3 、Ni2 几乎被聚乙二醇相完全萃取 ,Cd2 几乎不被萃取。〔结论〕实现了上述五种离子混合溶液中Cd2 的定量分离。