ALT低于2倍正常值上限的慢性HBV感染者肝脏病理结果的预测因素分析

目的探索在ALT2倍正常值上限(2×ULN)的慢性HBV感染人群中,一般临床指标对肝脏病理结果的预测作用。方法收集2009年1月至2013年6月新乡医学院第一附属医院收治的122例ALT2×ULN的慢性HBV感染者,在超声引导下行肝穿刺活组织检查术,判断肝组织炎症活动度及纤维化程度,同期化验肝功能、乙型肝炎血清标志物、HBV DNA等指标,应用Logistic回归分析法探索该类患者的一般临床指标对其肝脏病理结果的预测作用。结果 122名患者中有明显炎症或纤维化(G≥2或S≥2)者共94例(77.0%),早期肝硬化者5例(4.1%)。G2组与G≥2组相比,除HBV DNA外,其余各指标间差异均无统计学意义;S2组与S≥2组相比,年龄、HBeAg、HBV DNA、AST、血小板差异均有统计学意义;Logistic回归分析提示,年龄、HBeAg和AST是肝脏明显纤维化(S≥2)的独立预测因子。结论对血清ALT2×ULN的慢性HBV感染者,年龄40岁、HBeAg阴性、AST40U/L者应积极进行肝穿刺活组织检查术,必要时尽早抗病毒治疗。     

经病理诊断的慢性乙型肝炎病毒携带者与慢性肝炎的临床分析

目的 比较病理学诊断为慢性HBV携带者与慢性肝炎患者的临床资料,为不同ALT水平的慢性HBV感染者的处理提供病理学依据.方法 对292例慢性HBV感染者进行肝活组织检查,按病理学诊断符合慢性HBV携带者标准(G0～G1且S0～S1)与慢性肝炎标准[G＞1和(或)S＞1]分为携带组和肝炎组,比较不同年龄分层及ALT水平分层与病理诊断的关系,同时比较其他可能与病理诊断相关的临床、生物化学及影像学指标,Logistic回归方程(后退法,极大似然法)进行多因素分析,确定病理学符合慢性HBV携带者诊断的独立影响因素.结果 292例患者中,病理诊断为慢性HBV携带者有140例,占47.9％;慢性肝炎152例,占52.1％.HBV携带组与慢性肝炎组在≤35岁与36～40岁、＞40岁比较,差异有统计学意义(x2=3.936,8.534;P=0.047,0.003); ALT水平在＜0.5×正常值上限(ULN)、(0.5～1.0)×ULN、(1.1～1.5)×ULN、(1.6～2.0)×ULN、＞2.0×ULN间比较差异有统计学意义(x2=55.314,P＜0.01),但ALT在(1.1～1.5)×ULN与＞2.0×ULN比较,差异无统计学意义(x2=3.810,P=0.051).多因素分析显示,病程、饮酒史、ALT分层、HBV DNA水平及超声检查肝表面是否光滑是病理学符合慢性HBV携带者诊断的独立影响因素(OR=0.995、0.224、0.516、1.308、0.270,P=0.005、0.007、0.000、0.025、0.001).结论 年龄35岁以上且ALT水平介于(1～2)×ULN的患者行肝活组织检查的临床意义更大.     

ALT正常的慢性HBV感染者临床特征与肝组织学相关性研究

目的:探讨丙氨酸氨基转移酶(ALT)正常的慢性HBV感染者临床特征与肝脏病理的相关性。方法:对ALT正常的105例慢性HBV感染者进行肝组织病理检查,并对血清HBV标志物(HBV-M)和HBV DNA等进行分析。结果:105例慢性HBV感染者中肝组织有病理改变者占93.3%(98/105),其中G2及G2以上者占17.1%(18/105),S2及S2以上占21.9%(23/105),且12.4%(13/105)的患者组织学表现为肝硬化。年龄与纤维化分期呈正相关(rs=0.21,P<0.05);0.5×ULN0.05),但肝纤维化分期有差异显著性意义(P<0.05)。肝组织纤维化程度还与年龄和HBV DNA复制水平有关,而与HBe Ag阳性与否无相关性。结论:部分ALT水平正常的慢性HBV感染者肝组织病变也处于活动期,其中年龄>41岁、0.5×ULN相似文献   

171例慢性乙型肝炎病毒感染者临床与肝组织病理分析

目的:观察171例HBV DNA阳性的慢性乙型肝炎病毒(HBV)感染者临床检测指标与肝脏组织病理的关系.方法:将171例患者分为4组,A组丙氨酸氨基转移酶(ALT)≤0.5 ×ULN(正常值上限),B组0.5 ×ULN＜ALT≤1×ULN,C组1×ULN＜ ALT≤2×ULN,D组2×ULN＜ ALT≤5 × ULN,观察各组患者肝脏炎症分级(G)和纤维化分期(S)的差异,进一步分析各组炎症分级和纤维化分期与其他肝脏生化学指标如天门冬氨酸氨基转移酶(AST)、血小板(PLT).及肝纤维化模型(APRI,以AST/PLT比值的统计量作为数字化模型)的关系.结果:A组25％患者肝组织炎症为G2,B组和C组分别有16.4％、49.2％患者肝组织炎症分级为G2～G3,不同ALT组间肝组织炎症分级的差异有显著性意义(P＜0.05),随着ALT水平升高,肝组织G2～G3检出比例增加;171例患者不同肝组织炎症分级组,球蛋白(Glo)、AST、γ-谷氨酰转肽酶(GGT)、PLT、APRI的差异均有显著性意义(P＜0.05);不同肝组织纤维化分期组,年龄、HBeAg状态、白蛋白(Alb)、AST、GGT、PLT、HBV DNA、APRI的差异均有显著性意义(P＜0.05),其中随着纤维化程度进展,AST、APRI值逐渐升高,Alb、PLT逐渐下降.结论:HBV DNA阳性的慢性HBV感染者,即使ALT正常,仍有不同程度的肝组织炎症和纤维化改变,需综合观察年龄、AST、GGT、APRI、HBeAg状态和HBV DNA水平,必要时建议患者行肝脏穿刺病理检查,以准确了解肝脏疾病进展.     

HBeAg阳性和阴性慢性乙型肝炎患者肝组织学与血清学关系的比较

目的 研究慢性乙型肝炎(CHB)患者(1×ULN≤ALT≤2×ULN)肝组织学与血清学的关系.方法 对全国多中心516例CHB患者(1×ULN≤ALT≤2×ULN)行血清学及肝穿刺病理检查,依据血清HBeAg表达水平将患者分为HBeAg阳性组和HBeAg阴性组,分析两组肝组织病理学与血清学指标的相关性.结果 HBeAg阳性患者肝组织炎症分级和肝纤维化分期与血清HBsAg和HBeAg表达水平呈负相关(P＜0.01),与血清透明质酸(HA)和α2-巨球蛋白水平呈正相关(P＜0.01),与血清HBVDNA水平无明显相关性;HBeAg阴性患者肝组织炎症分级和肝纤维化分期与血清HBV DNA水平呈正相关(P＜0.01),与HBsAg、HA和α2-巨球蛋白等无明显相关性.结论 血清HBV DNA可做为HBeAg阴性CHB患者肝脏病变程度的有效预测指标,HBsAg、HBeAg、HA和α2-巨球蛋白均可做为HBeAg阳性CHB患者肝组织损伤的预测指标.     

超声引导下经皮肝脏穿刺活检的临床价值

目的探讨超声引导下经皮肝脏穿刺活检的临床价值。方法采用PHILIPS-HD11超声仪、3.5MHz高频探头、Bard活检枪及活检针对肝脏进行穿刺,对穿刺组织进行病理学检查。结果46例患者均穿刺成功,获取肝组织108块。病理学检查诊断为慢性乙型肝炎38例,慢性丙型肝炎4例,肝细胞癌1例,原发性胆汁性肝硬化1例,自身免疫性肝炎肝硬化1例,D-J综合征并成人特发性胆管缺失综合征1例。结论超声引导下经皮肝脏穿刺活检能确定肝脏损害的病理性质,对指导临床诊断和治疗有重要的应用价值。     

肝穿刺活组织病理检查在肝病诊治中的意义

目的评价肝穿刺活组织检查在肝病诊断及治疗中的价值。方法回顾性分析浦东新区南华医院自2007年以来接受B超定位下行1秒钟(1s)肝穿刺诊断并指导治疗患者的情况。结果 16例不明原因肝损伤的患者经肝穿刺活组织检查明确诊断,药物性肝炎4例(4/16,25%),脂肪性肝炎4例(4/16,25%)自身免疫性肝炎2例(2/16,12.5%),乙型肝炎2例(2/16,12.5%),急性淤胆型肝炎、血吸虫性肝病各1例,2例经肝穿活检仍未能明确诊断。在20例反复肝功能异常半年以上、ALT<2倍正常上限值(2×ULN)、高病毒载量的慢性乙型肝炎(CHB)患者中,有65%(13/20)肝脏炎症指数≥2级,需抗病毒治疗,且年龄20～40岁者占69.2%(9/13)。结论肝穿刺活检是诊断肝功能异常病因的重要手段,对明确诊断并确立最适当的治疗方案有重要价值。     

无症状HBV携带者血清HBV DNA水平与肝组织炎症活动度的关系

目的探讨无症状HBV携带者(AsC)肝脏病理损害程度与血清HBV DNA水平的关系.方法对139例AsC于超声引导下采用美国生产的DARD自动活检枪经皮肝穿刺行病理学检查;HBV DNA用PCR测定.结果139例AsC中G1者94例,G2者26例,G3者19例,无G0和G4者;血清HBV DNA水平与肝组织炎症活动度间无显著性差异(P＞0.05);HBeAg阴性组G3者所占比例大于HBeAg(+)组(P=0.019).结论AsC肝脏均有不同程度损害,其损害程度与HBV DNA水平无相关性.     

术后即刻彩色多普勒超声检查对肝活检并发出血的诊断及其引导压迫止血

目的研究超声引导下肝活检术中, 组织取材后即刻行彩色多普勒超声检查对肝脏损伤性出血的诊断价值, 研究超声引导下穿刺点局部压迫止血的临床效果。方法 132例各种肝脏疾病住院患者行超声引导下肝穿刺活组织检查, 包括61例弥漫性肝实质病变和71例肝脏局灶性病变, 于肝活检后即刻行彩色多普勒超声检查, 观察穿刺部位的异常血流信号, 如有出血性信号则行超声引导下压迫止血, 直至出血信号消失。计量资料比较用方差分析, 计数资料比较用χ2检验。结果即刻彩色多普勒超声检查显示弥漫性肝病组和局灶性肝病组分别有36.1%(22/61)和40.8%(29/71)的病例出现损伤性出血。进行超声引导下的局部压迫止血, 所有的出血信号最终都消失, 2组的止血时间中位数均为2 min, 2组间出血率和止血时间差异均无统计学意义(P>0.05)。无严重并发症和死亡发生。结论沿穿刺针道的肝脏损伤性出血在肝活检术中是常见的伴随情况, 即刻彩色多普勒超声检查能够及时发现出血信号, 并引导有效压迫止血, 即刻彩色多普勒超声检查应成为肝活检术中取材后的常规措施, 可能会有助于避免严重出血并发症的发生。     

超声引导经皮肺穿刺活检在近胸壁肺部肿块诊断中的价值

目的 探讨超声引导下经皮肺穿刺活检对肺外周型病变定性诊断的应用价值.方法 在超声引导下对45例胸部影像学检查显示的肺周边型肿块需明确诊断者行超声引导下经皮肺穿刺活检术.结果 本组45例患者中43例(95.6％)经组织学检查,获得了明确的病理诊断,病种包括肺癌32例(71.1％),其中鳞癌14例,腺癌7例,小细胞癌7例,大细胞癌4例;炎症9例(20.0％),不典型增生2例(4.4％);另外有2例病理检查诊断结果为“正常肺组织”等,未能明确定性诊断.并发咯血1例,并发症发生率为2.2％.结论 超声引导经皮在胸膜及肺周围病变穿刺活检术操作简单、取材准确、诊断准确性高、安全、创伤小、并发症少,具有实际应用价值,值得临床广泛推广.     

Comparative Study of an Ultrasound-guided Percutaneous Biopsy and Endoscopic Ultrasound-guided Fine-needle Aspiration for Liver Tumors

Objective Both a percutaneous biopsy and endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) have been widely performed for liver tumors. However, no studies have compared these two biopsy methods. Method A retrospective study was conducted using medical records for patients who underwent a liver tumor biopsy from 2012 to 2019. The cases were classified into two groups for a comparison: an ultrasound-guided percutaneous biopsy group (percutaneous group) and an EUS-FNA group (EUS group). Results A total of 106 patients (47 in the percutaneous group and 59 in the EUS group) were included. The final diagnosis was malignant in 100 cases and benign in the remaining 6 cases. While the median lesion diameter was 62 mm in the percutaneous group, it was significantly smaller (34 mm) in the EUS group (p <0.01). The EUS group had more left lobe tumors than right lobe tumors. All cases of caudate lobe tumor (four cases) underwent EUS-FNA. The sensitivity, specificity, and accuracy of the procedure were 95%, 100%, and 96% in the percutaneous group and 100%, 100%, and 100% in the EUS group, respectively showing no significant difference. Adverse events were reported in 17% of the percutaneous group, which was significantly lower than in the EUS group (2%; p <0.01). Conclusion A percutaneous biopsy and EUS-FNA have equivalent diagnostic qualities for liver tumors, although EUS-FNA tends to be associated with fewer adverse events. A complete understanding of the characteristics of each procedure is essential when choosing the best biopsy method for each particular case.     

Ultrasonography in predicting and screening liver cirrhosis in children： A preliminary study

AIM: To evaluate the value of ultrasonography in predicting and screening liver cirrhosis in children. METHODS: Twenty-eight children with liver cirrhosis of various etiologies were examined by routine ultrasonography. A percutaneous liver biopsy guided by ultrasound was also performed on each patient, and the results of liver biopsy and ultrasonography were compared. RESULTS: When compared with the biopsy results, ultrasonography in combination of clinical and laboratory findings gave accurate diagnoses of children liver cirrhosis. Although ultrasound imaging of children with liver cirrhosis revealed abnormal characteristics, these images were not specific to this disease, thus reinforcing the necessity of ultrasound-guided liver biopsy in the diagnosis of children liver cirrhosis. CONCLUSION: Ultrasonography is reliable in the diagnosis of children liver cirrhosis, and its usefulness should be stressed in the screening and follow-up of high-risk pediatric patients.     

Role of liver biopsy in the management of chronic liver disease: selective rather than routine

Liver biopsy has historically played a central role in the diagnosis and management of a variety of chronic liver diseases. However, as the understanding of disease pathology has progressed, and laboratory diagnostics, imaging technology, and clinical algorithms to determine both the etiology and presence of fibrosis have advanced, the role of liver biopsy has become more circumscribed. In chronic liver disease, liver biopsy is now more often used selectively, rather than routinely, for diagnostic purposes. Newer treatment of chronic hepatitis B and C has become more effective and thus reduced the routine need to acquire tissue. Risk factors for nonalcoholic fatty liver disease are readily identified and suggest the diagnosis after exclusion of alternative considerations, and there is no specific treatment for this condition; thus there is little role for the routine use of liver biopsy to guide treatment. Only in select cases of chronic hepatitis C, especially in patients with genotype 1, an indeterminate stage and grade of disease on noninvasive evaluation, or in those with human immunodeficiency virus coinfection, for whom the risks and benefits of treatment are less clear, is there a role for routine pretreatment biopsy.     

CT引导下经皮肝穿刺活检术在不明原因肝功能异常诊断中的临床应用

目的探讨CT引导下经皮肝穿刺活检术在不明原因肝功能异常诊断中的临床应用。方法回顾性分析58例不明原因肝功能异常患者行CT引导下经皮肝穿刺活检术的临床、病理资料,主要观察术后病理诊断率、GS分级诊断率、并发症。结果本组取材成功率100%,手术平均时间11.52 min,取得组织条(1.48±0.50)条,病理诊断率为84.5%(49/58),病理肝炎活动度(G)和肝纤维化程度(S)分级诊断率为72.4%(42/58)。经皮肝穿刺操作未导致肝功能指标数值(ALT、AST、ALP、GGT、TBIL)在短期内显著性增高。8例(13.8%)肝包膜下少量出血,经内科治疗后无进展。结论CT引导下经皮肝穿刺活检术对不明原因肝功能异常的病理及GS分级诊断率高,并发症可控,具有一定的临床应用价值。     

When is liver biopsy needed in the diagnosis of primary biliary cirrhosis?

BACKGROUND & AIMS: Liver biopsy has been largely replaced by less invasive measurements applied to mathematical models in defining the prognosis of patients with primary biliary cirrhosis (PBC). The need for liver biopsy in the diagnosis of patients with positive antimitochondrial antibodies (AMAs) in the setting of biochemical cholestasis remains uncertain. This study was undertaken to determine which variables indicate the need for liver biopsy in patients with positive AMA and suspected PBC. METHODS: A total of 3198 patients that were tested for AMA in 1999 and 2000 were identified. Of these, 198 (6.2%) were AMA positive. Forty-two patients were excluded because of unavailable laboratory data or liver biopsy specimens. Data on the remaining 156 patients were analyzed to determine which variables among patients with positive AMA with and without a histopathologic diagnosis of PBC indicate the need for liver biopsy. RESULTS: In 131 of 156 (84.6%) patients, a diagnosis of PBC was established by liver biopsy. The histological diagnosis of PBC was associated with a cholestatic biochemical profile (alkaline phosphatase [AP] >1.5 times the upper limits of normal [ULN] and aspartate aminotransferase [AST] <5 times ULN) in 112 of 131 (85.5%) patients. The combination of AP >1.5 times the ULN and AST <5 times the ULN yielded a 98.2% positive predictive value of PBC diagnosis on liver biopsy in AMA-positive subjects. These results were corroborated by cross-validation in an independent set of patients. CONCLUSIONS: In patients with positive AMA, a cholestatic biochemical profile (AP >1.5 times the ULN), and absence of markedly elevated AST (<5 times the ULN), liver biopsy is rarely required to establish the diagnosis of PBC. Liver biopsy would be beneficial to establish the diagnosis of PBC in only a minority of AMA-positive patients with AP <1.5 times the ULN or AST >5 times the ULN.     

Portal vein thrombosis complicating hepatocellular carcinoma. Value of ultrasound-guided fine-needle biopsy of the thrombus in the therapeutic management

Abstract: During a 4-year period portal vein thrombosis was diagnosed in 20 Child class A patients with cirrhosis by means of ultrasound and ultrasound-Doppler study. Seventeen of them showed single or multiple focal liver lesions diagnosed as hepatocellular carcinoma by ultrasound-guided fine-needle biopsy and the remaining three a coarse liver echo-pattern without focal lesions. One patient was found to have developed portal vein thrombosis after the fifth ethanol injection of a single hepatocellular carcinoma lesion 17 mm in diameter. Ultrasound-guided fine-needle biopsy of the thrombus was performed on all the patients: portal vein thrombosis was neoplastic in 13 cases and non-neoplastic in seven cases (five patients with a single lesion; one with two lesions; one with coarse liver echo-pattern). Among the five patients with a single lesion, one had already been treated by percutaneous ethanol injection therapy. There were no complications related to the biopsy procedures. The diagnosis of non-neoplastic thrombosis allowed five new patients to be recruited for percutaneous ethanol injection treatment and allowed it to continue in the patient with portal vein thrombosis occurring after the fifth ethanol injection. The routine use of ultrasound-guided fine-needle biopsy of portal vein thrombosis yields an accurate diagnosis of the nature of the thrombus and can improve the selection for percutaneous ethanol injection treatment of patients with cirrhosis with hepatocellular carcinoma lesions.     

肝活检对转氨酶正常的慢性乙型肝炎感染的临床意义

目的探讨肝活检组织学对转氨酶持续正常6个月以上的慢性乙型肝炎(CHB)感染的临床意义.方法共452例乙型肝炎病毒(HBV)慢性感染患者接受快速经皮肝穿刺,全部病例血清HB-sAg均为阳性,病程超过6个月.肝组织切片由病理科医生盲法进行阅片.结果所有患者肝组织学显示肝内均有炎症、坏死存在.血清转氨酶异常组的炎症分级(G)和纤维化分期(C)均较转氨酶正常组严重(P＜0.05),但在后一组中,HBeAg阳性和阴性组中仍分别有10例(5.5%)和13例(9.1%)为G3,分别有7例(3.8%)和16例(11.1%)为S3,分别有3例(1.6%)和7例(4.9%)为S4.在转氨酶正常病例中,HBeAg阴性组纤维化程度较HBeAg阳性组严重(P＜0.05).如将HBeAg阳性组和阴性组合并统计,则在转氨酶正常者中,有14.6%的患者肝炎症坏死分级为G3,有21.4%为S3和S4.结论虽然严重肝病理改变多见于转氨酶异常的HBV慢性感染,但也有不少转氨酶正常病例肝呈现明显组织学异常.肝活检可作为判断肝病活动性、纤维化程度和抗病毒治疗的根据.在转氨酶正常的病例,如果肝炎症坏死-纤维化较严重,进行适当的处理看来是必要的.     

Transjugular liver biopsy with an automated trucut-type needle: comparative study with percutaneous liver biopsy.

OBJECTIVE : Transjugular liver biopsy using the suction method usually produces small specimens with excessive fragmentation, hence the diagnosis adequacy of specimens and the clinical impact of performing the biopsy have been questioned. An alternative biopsy needle, the Quick-Core needle system, which uses an automated trucut-type mechanism, has been shown to produce non-fragmented tissue specimens. The aim of the present study was to evaluate the safety, adequacy and clinical impact of the transjugular liver biopsy by comparing it with the standard percutaneous liver biopsy. DESIGN : We recruited all patients who underwent liver biopsies by percutaneous or transjugular routes in the Department of Medicine, Princess Margaret Hospital, Hong Kong between January 1998 and December 1999. METHOD : We recorded demographics and clinical features of patients, indications and complications, and the clinical impact of the liver biopsy procedure. All liver biopsy specimens were reviewed by the histopathologist, who was blinded to the approach of taking the biopsy. All variables between patients undergoing transjugular and percutaneous liver biopsies were compared. RESULTS : During the study period, 50 percutaneous and 18 transjugular liver biopsies were performed. All transjugular liver biopsies were performed successfully with adequate tissue for diagnosis. Although specimens obtained by the transjugular technique tended to be shorter (10 mm v. 18 mm by the percutaneous approach, P < 0.001), the presence of fragmentation was similar to that in biopsies obtained by the percutaneous approach. Respectively, 100% and 98% of specimens obtained by the transjugular and percutaneous approaches were considered to be adequate for histological assessment. The clinical impact of transjugular and percutaneous liver procedures was comparable (89% v. 76%, P = 0.25). CONCLUSION : Specimens obtained by a transjugular automated trucut needle are sufficient for histological assessment, and carry clinical impact in patient management.     

3种不同肝活组织检查方法的并发症分析

目的探讨3种肝活组织检查方法并发症的发生率。方法1995-2006年,共行肝活组织检查1557例。其中友谊医院患者1031例,内蒙古兴安盟医院患者468例,河北沧州传染病医院患者48例,广东廉江市人民医院患者10例。年龄12～70岁,平均(36±12)岁。肝活组织检查均由钱林学操作。本研究分3组,即盲穿组、半盲穿组和超声引导组,观察3种方法肝活组织检查并发症的发生率,包括:死亡、出血、气胸、疼痛、胆汁性腹膜炎和血管迷走神经反射。结果1559例患者中,只有1例患者死亡,发生在盲穿组。总出血率为0.45%(7/1557),盲穿组为0.51%(4/783),半盲穿组为0.41%(2/485),超声引导组为0.35%(1/289)。盲穿与其他两组比较,差异有统计学意义(P<0.05),而半盲穿和超声引导相比,差异无统计学意义。气胸总发生率为2.25%(35/1557),盲穿组为2.94%(23/783),半盲穿为2.47%(12/485),两组差异无统计学意义,超声引导组为0%。严重疼痛总发生率为3.08%(48/1557),盲穿组为3.58%(28/783),半盲穿为3.51%(17/485),两组差异无统计学意义,超声引导组疼痛为1.04%(3/289),与其他两组比较,差异有统计学意义(P<0.05)。结论超声引导肝活组织检查是目前最安全、最可靠方法,建议临床尽量采用超声引导肝活组织检查。     

47例肝脏占位性病变患者肝脏病理检查与血生化联合检测的对比研究

目的评价肝脏活组织病理检查对早期诊断原发性肝癌的临床价值及其与血清甲胎蛋白（AFP）、碱性磷酸酶（AKP）、γ-谷氨酰转移酶（GGT）指标和影像学的相关性,以提高原发性肝癌的早期诊断率。方法对47例影像学检查提示肝脏占位性病变并进行肝活检患者进行回顾性分析。结果临庆诊断为原发性肝癌的患者47例,病理检查证实28例,临床诊断与病理检查符合率为59．60％,1例外科手术切除前肝活检未查见癌细胞的患者,术后证实为胎儿型肝母细胞瘤,有2例做肝活检2次,有2例做肝活检3次而查见癌细胞。结论肝活检是诊断原发性肝癌的金标准,但在临床应用过程中存在客观误差,需多次多部位多方向取材,并结合血生化指标及影像学检查,以提高早期肝癌的诊断率。