EB 病毒与非霍奇金淋巴瘤——附180 例报道

 目的　探讨 NHL与 EBV感染的相关性。方法　用 ISH方法检测 1 80例 NHL中EBV编码的 RNA( EBER1 /2 )。结果　 ( 1 ) EBER1 /2感染率为 42 .8% ( 77/1 80 ) ,感染率依次为TCL64.8%、NK/T46.2 %、BCL9.5 %。其中 TCL、NK/T与 BCL相比 ,P≤ 0 .0 0 5 ;( 2 )结内、结外EBV感染率依次为 2 2 .2 %、5 3.8% ,有显著差异 ,P<0 .0 0 5 ;( 3)面中线 (鼻腔 /口咽环 ) EBER1 /2感染率 72 .4% ( 5 5 /76) ,多形细胞性 EBER1 /2感染率 70 .1 % ( 5 4 /77)。结论　本地区 NHL与 EBV感染关系密切 ,TCL、NK/T淋巴瘤感染率明显高于 BCL,而且有部位限制性和亚型倾向性.     

EBER1/2、LMP-1在NK/T细胞淋巴瘤中的表达

目的　探讨NK /T细胞淋巴瘤与EB病毒 (EBV )的关系。方法　收集 2 6例NK /T细胞淋巴瘤 (淋巴结内 10例 ,淋巴结外 16例 ) ,采用免疫组织化学S P法确定瘤细胞本质 ,用原位杂交法检测EBV编码的RNA (EBER 1/2 )。结果　①在 2 6例NK /T细胞淋巴瘤中 ,EBER1/2检出率为 46 .2 % (12 /2 6 ) ,其中淋巴结内检出率为 10 .0 % (1/10 ) ,淋巴结外检出率为 6 8.8% (11/16 ) ,2组比较有非常显著性差异 (P <0 .0 1)。②在淋巴结外NK/T细胞淋巴瘤中 ,EBER 1/2在鼻腔检出率为 90 .0 % (9/10 ) ,其它部位为 33 .3 % (2 /6 ) ,2组比较有显著性差异 (P <0 .0 5 )。③EB病毒潜伏膜蛋白 (LMP 1)在EBER1/2阳性病例中的检出率为16 .7% (2 /12 )。结论　EBV在NK /T细胞淋巴瘤的发生中有一定作用 ,并具有部位依赖性的特点 ;EBV在NK /T细胞淋巴瘤中的潜伏感染模式不完全一致。     

鼻NK/T细胞淋巴瘤的免疫表型、EBV感染及TCRγ基因重排的检测

 目的 检测鼻NK/T细胞淋巴瘤（NK/TCL）的免疫表型、EBV感染及TCRγ基因重排,为诊断和鉴别诊断提供依据。方法 收集诊断鼻NK/TCL48例患者石蜡包埋标本,用免疫组化SP法标记LCA、CD79α、CD20、CD56、CD3、CD45RO及EBV抗体研究其免疫表型;EBER探针原位杂交方法检测EBV编码的小分子RNA（EBER）;聚合酶链式反应扩增方法检测TCRγ基因重排。结果 48例鼻NK/TCL均表达LCA,CD3、CD45RO、CD56和EBV阳性率分别为44%、52%、73%和19%,CD79α和CD20均阴性;EBER阳性率为81%;TCRγ基因重排阳性率为19%。结论 鼻NK/TCL免疫表型不一致,并非所有病例CD56阳性,石蜡切片中CD3阳性定位于细胞质;EBER在肿瘤细胞中高表达,提示它们可能为NK细胞来源;部分TCRγ基因重排阳性病例应为鼻NK样T细胞淋巴瘤。     

鼻腔NK/T细胞淋巴瘤临床病理特征及诊断分析

目的 探讨鼻腔NK/T细胞淋巴瘤的临床、病理特征,诊断和误诊原因.方法 复习62例鼻腔NK/T细胞淋巴瘤的临床资料,应用组织病理学、免疫组化和原位杂交技术分析其临床特点、诊断、病理特征和免疫表型.结果 鼻腔NK/T细胞淋巴瘤以鼻塞、涕血和头痛为主要症状,表现为面部中线破坏性病变.病理改变为肿瘤性淋巴样细胞弥漫或散在分布于凝固性坏死和多种炎细胞混合浸润的背景上.所有病例表达T细胞标记,不表达B细胞标记;NK细胞标记阳性5例;EB病毒mRNA阳性56例(90%).结论 鼻腔NK/T细胞淋巴瘤临床表现不典型,易误诊误治.免疫组化检查是确诊的重要方法.患者以T细胞为主,大多有EBV感染.     

鼻型T/NK细胞淋巴瘤累及皮肤1例报道

[目的]探讨鼻型T/NK细胞淋巴瘤累及皮肤的临床表现、病理、免疫表型及EB病毒相关性.[方法]应用免疫组织化学染色,选用CD45RO、CD3、TIA-1、CD20、Ki-B5、CD68和LMP1等抗体;用E-BER1/2原位杂交检测EB病毒编码的小分子RNA.[结果]此例皮肤及鼻部取材组织病理表现为坏死及中等大小的异形性肿瘤细胞增生浸润,CD56 ,CD3 ,CD8,CD20-,为T/NK细胞淋巴瘤表型.EBV瘤细胞阳性,进一步证实为鼻型T/NK细胞淋巴瘤,EBV相关性.[结论]支持鼻型T/NK细胞淋巴瘤是具有特殊免疫表型,与EB病毒有高度相关性的独立疾病.     

皮下脂膜炎样T细胞淋巴瘤与皮肤的结外鼻型NK/T细胞淋巴瘤的对比研究

目的:通过对20例皮下脂膜炎样T细胞淋巴瘤(subcutaneous panniculitis-like T-cell lymphoma,SPTL)和19例皮肤的结外鼻型NK/T细胞淋巴瘤的对比研究,加深对2者的认识.方法:从临床病理、免疫标记、EB病毒(Epstein-Barr virus,EBV)感染和T细胞受体(T-cell receptor, TCR)基因重排等多个方面对2者进行比较.结果: 临床表现上2者不易鉴别,但皮肤NK/T细胞淋巴瘤常伴皮肤外播散、预后差;组织学上,SPTL常严格局限于皮下脂肪组织,而皮肤NK/T细胞淋巴瘤以真皮为中心形成弥漫性浸润,常累及皮下脂肪层,更易于见到大片凝固性坏死、血管中心性浸润和亲表皮现象;免疫表型上,SPTL常表达βF1、膜型CD3、CD8,不表达CD4、CD56,而大部分皮肤NK/T细胞淋巴瘤则表达CD56和细胞质CD3ε,仅少数表达膜型CD3、CD8.CD56、CD3、CD8和βF1的表达差异有统计学意义(P<0.05).SPTL患者中检出 EBER1/2原位杂交阳性,而皮肤NK/T细胞淋巴瘤100%病例为阳性,2者比较差异有统计学意义(P<0.05).SPTL患者中检出TCR-γ基因克隆性重排,而皮肤NK/T细胞淋巴瘤患者仅有4/18例(22.2%)检出重排,2者之间差异有统计学意义(P<0.05).结论:有无皮肤外播散,组织学上有无大片凝固性坏死、血管中心性浸润和亲表皮现象,是否表达免疫组织化学标记CD56、CD3、CD3ε、CD8和βF1,EB病毒原位杂交阳性与否,以及TCR-γ克隆性重排检出与否,均可作为SPTL和皮肤NK/T细胞淋巴瘤的鉴别要点.准确鉴别2者需综合临床、组织病理学、免疫表型、EB病毒感染和基因重排等结果进行全面分析.     

不同部位和类型恶性淋巴瘤与EBV感染相关性研究

目的：对不同部位和类型的恶性淋巴瘤中EB病毒（Epstein-Barr virus，EBV）的感染情况进行检测和分析，探讨EBV感染可能与恶性淋巴瘤的病因关系。方法：收集恶性淋巴瘤组织标本127例，其中鼻腔及鼻咽淋巴瘤60例，胃淋巴瘤30例，表浅淋巴结淋巴瘤37例。组织病理学诊断为非霍奇金淋巴瘤（NHL）108例，霍奇金淋巴瘤（HD）19例。采用原位分子杂交方法检测淋巴瘤组织中EBV编码的小RNA（EBER），确定EBV在恶性淋巴瘤细胞中的存在。结果：108例NHL（包括鼻腔及鼻咽、胃、表浅淋巴结部位），EBER检测46例阳性，阳性率为42.6%。鼻腔/鼻咽NHL的EBER阳性率为58.3%（35/60），其中NK/T细胞淋巴瘤29例，EBER阳性19例，阳性率为65.5%（19/29）；B细胞淋巴瘤31例，EBER阳性16例，阳性率为51.6%（16/31）。胃部NHL的EBER阳性率为30.0%（9/30），其中B细胞淋巴瘤28例，EBER阳性9例，阳性率为32.1%（9/28）；T细胞淋巴瘤2例，均为EBER阴性。表浅淋巴结NHL18例，EBER阳性2例，阳性率为11.1%（2/18）；表浅淋巴结HD19例，EBER阳性5例，阳性率为26.3%（5/19）。结论：本组资料非霍奇金淋巴瘤EBV感染阳性率（42.6%）略高于霍奇金淋巴瘤EBV感染阳性率（26.3%），差异无显著性意义（P〉0.05）。鼻腔及鼻咽NHL的EBV感染阳性率（58.3%）高于胃NHL（30.0%）和表浅淋巴结NHL（11.1%）（P〈0.05）。研究提示各类型淋巴瘤与EBV感染均有密切关系，且存在部位依赖性。     

不同类型淋巴瘤438例EB病毒分布特点

目的 探讨不同类型淋巴瘤中EB病毒(EBV)的分布特点.方法 选用免疫组织化学EnVision法标记ALK1、CD3、CD5、CD7、CD10、CD15、CD20、CD23、CD30、CD38、CD43、CD56、CD68、CD79、CD99、CyclinD1、EMA、TdT与EBV潜伏膜蛋白(LMP-1),原位杂交技术标记EBV编码的RNA(EBER).按照2008年WHO淋巴造血系统肿瘤分类标准对存档的438例淋巴瘤组织标本重新分类.结果 在B细胞非霍奇金淋巴瘤(B-NHL)、NK/T细胞淋巴瘤和霍奇金淋巴瘤(HL)中EBER阳性率分别是5.4％(14/261)、16.5％(19/115)和59.7％(37/62),LMP-1阳性率分别为5.4％(14/261)、5.2％(6/115)和59.7％(37/62).弥漫大B细胞淋巴瘤(DLBCL)患者中,EBER在60岁以上组中的阳性率(13.2％,7/53)明显高于60岁及以下组(1.2％,1/81)(P＜0.05);在NK/T细胞淋巴瘤中LMP-1蛋白表达与EBER表达具有不一致性,EBER阳性率明显高于LMP-1(P＜0.05);5例鼻型NK/T细胞淋巴瘤中全部表达EBER;在HL淋巴瘤中LMP-1蛋白表达与EBER表达具有一致性.结论 EBV感染与淋巴瘤类型相关,EBV在鼻型NK/T细胞淋巴瘤中表达率高,HL次之.鉴于EBER与LMP-1在HL中的一致性表达,出于经济学考虑,LMP-1可代替EBER用作EBV的检测.EBV可能在鼻型NK/T细胞淋巴瘤、HL等的发生、发展中有重要作用.     

鼻腔NK/T细胞淋巴瘤的临床病理特点及治疗

原发鼻腔非霍奇金淋巴瘤(nonHodgkin’slymphoma,NHL)是亚洲、拉丁美洲和南美洲较常见的恶性淋巴瘤[18]。在中国,除韦氏环NHL外[9],鼻腔NHL是常见的结外NHL之一,占全部恶性淋巴瘤的2%～10%[1,35,10,11]。欧美鼻腔NHL极少见,文献中常将鼻腔和鼻窦NHL合并报道[1315]。鼻腔NHL可来源于自然杀伤(naturalkiller,NK)、T或B淋巴细胞,中国的鼻腔NHL以NK、T细胞淋巴瘤为主,B细胞淋巴瘤少见[1,4,5],相反,欧美的鼻腔NHL以B细胞来源多见[13,14]。在REAL淋巴瘤分类中,来源于NK、T细胞的原发鼻腔NHL是一种独立的病理亚型,并被命名为血…     

171例淋巴瘤病变组织中EB病毒表达情况的分析

目的:探讨不同类型淋巴瘤与EB病毒(Epstein-Barr virus,EBV)感染的关系。方法:收集淋巴瘤组织171例,包括弥漫大B细胞淋巴瘤(DLBC)106例;结外NK/T细胞淋巴瘤,鼻型22例;霍奇金淋巴瘤(HL)19例;血管免疫母细胞性T细胞淋巴瘤(AITL)13例;黏膜相关淋巴组织B细胞淋巴瘤(MALT)11例。应用EBV Lmp-1单抗免疫组化(IHC)和生物素标记的EBER1寡核苷酸探针原位杂交(ISH)分析EBV感染与淋巴瘤的关系。结果:淋巴瘤组织中EBV Lmp-1蛋白与EBER1 mRNA总阳性率分别为11.1%(19/171)、25.7%(44/171)。其中AITL为30.8%(4/13)、61.5%(8/13);HL为47.4%(9/19)、57.9%(11/19);结外NK/T细胞淋巴瘤为22.7%(5/22)、81.8%(18/22);DLBC为0.94%(1/106)、5.7%(6/106);MALT为0(0/11)、9.1%(1/11)。结果显示EBV在DLBC及MALT中的表达率低于AITL、HL及结外NK/T细胞淋巴瘤,差异有统计学意义(P0.05);且原位杂交检测EBER1 mRNA比免疫组化检测Lmp-1蛋白更为敏感(P0.01)。结论:EBV感染与淋巴瘤有密切关系,不同类型淋巴瘤与EBV感染的关系有差异。     

Primary Testicular NK/T-Cell Lymphoma：A Study of Two Cases and Review of Literature

Primary testicular NK/T-cell lymphoma is an extremely rare entity progressed rapidly. The aim of this study was to examine clinical and pathological features of primary testicular NK/T-cell lymphoma and to investigate the effective diagnosis and prognosis. In this paper, the two cases of primary testicular NK/T-cell lymphoma were observed by light microscopy, immunohistochemistry and examined by in situ hybridization for Epstein-Barr Virus (EBV) DNA and the literatures were reviewed. The two patients respectively present with bilateral and right-side painless testicular enlargement. The morphology of neoplastic cells of case 1 were small to medium, tumor cells of case 2 were small, medium and large mixed. The tumor cells grew diffusely with irregular and distort nuclear, destructed the organizational structure of the normal testis, and damaged blood vessels, meanwhile, coagulation necrosis was exist. Immunohistochemical staining of neoplastic cells showed positive for CD45, CD2, CD56, CD3ɛ (cytoplasm staining pattern), CD45RO and Granzyme B, and negative for CD57, CD20, CD79α, CD30, CK, MPO, TdT, Bcl-2 and PLAP were negative. In addition, the EBV DNA was detected in the lymphoma by In situ hybridization. In conclusion, the expression of CD56, CD3ɛ, and Granzyme B associated proteins and EBV examination by in situ hybridization play a vital role in diagnosis and differential diagnosis of primary testicular NK/T-cell lymphoma.     

Epstein-Barr virus in lymphoproliferative diseases in the sino-nasal region: Close association with CD56+ immunophenotype and polymorphic-reticulosis morphology

Association between Epstein-Barr virus (EBV) and nasal T-cell lymphoma (NTL) has been demonstrated. NTL has 2 types of histologic figures: one is ordinary non-Hodgkin's lymphoma (NHL) with monomorphous proliferation, and the other is polymorphic-reticulosis (PR) morphology. The presence of the EBV genome and its sub-types (A and B) were examined on paraffin-embedded specimens from 36 cases of sino-nasal lymphomas (SNL) collected from Seoul, Republic of Korea, where the frequency of NTL is high. Patients' ages ranged from 2 to 74 years (median 54 years) with a male-to-female ratio of 2.5:1. Immunophenotypically, 8 cases were B-cell type, 11 were T-cell type with CD56⁻, 14 were CD56⁺ type, and 3 were null-cell type. Five of 11 cases with ordinary NHL of T-cell type and 9 of 14 cases with PR were CD56⁺. The EBV genome was found by polymerase chain reaction (PCR) and in the tumor cells by in situ hybridization (ISH) in 1 of 4 B-cell type (25%), 5 of 10 T-cell type (50%), 11 of 13 CD56⁺ type (85%), and in both of null-cell type (100%). Of 16 cases with PR morphology, 15 (94%) were positive for the EBV genome. All of the 5 NTLs of ordinary NHL with CD56⁻ were negative for EBV. Concerning the sub-type of BV, 16 cases had type A, while none had type-B EBV. These findings suggest that NTL comprises 2 groups: EBV-positive NTLs are CD56⁺ and/or histologically PR, and EBV-negative NTLs are CD56⁻ and histologically ordinary NHL. The current results on Korean patients, together with earlier studies on Japanese and Malaysian patients, have shown the predominance of type-A EBV in sino-nasal lymphoma in Asia. © 1997 Wiley-Liss, Inc.     

Natural killer cell neoplasms

Natural killer (NK) cell tumors are an uncommon and heterogeneous group of disorders. The World Health Organization (WHO) classified mature NK cell neoplasms into 2 types: 1) extranodal NK cell lymphoma, nasal type and 2) aggressive NK cell leukemia. The mature NK cell tumors are prevalent in Asia and Central and South America. These tumors show polymorphic neoplastic infiltrate with angioinvasion and/or angiodestruction, cytoplasmic azurophilic granules, CD2-positive (CD2+)/CD3-negative (CD3-)/cCD3epsilon+/CD56+ phenotype, and strong association with Epstein-Barr virus (EBV). Loss of chromosomes 6q, 11q, 13q, and 17p are recurrent aberrations. Although blastic NK cell lymphoma, currently referred to as CD4+/CD56+ hematodermic neoplasm, also was included in the NK cell lymphoma category in the WHO classification scheme, existing evidence indicates a plasmacytoid dendritic cell derivation as opposed to an NK cell origin. Recently, rare cases of CD56+ immature lymphoid tumors have been reported in the literature. These tumors are characterized by blastic appearance, CD3-/CD4-/CD56+/CD13-/CD33- phenotype, T-cell receptor and immunoglobulin genes in germline configuration, and no evidence of EBV, suggesting a true immature NK cell derivation. For this article, the authors reviewed the recent concepts and progress in clinicopathologic features, pathogenesis, genetic characteristics, diagnosis, differential diagnosis, treatment approaches, and outcomes of all subtypes of NK cell neoplasms.     

EB病毒潜伏膜蛋白1基因C末端30 bp缺失与非霍奇金淋巴瘤的关系

背景与目的EB病毒(Epstein-Barr virus,EBV)与多种肿瘤包括非霍奇金淋巴瘤(non-Hodgkin'slymphoma,NHL)的发生有着密切关系,其中潜伏膜蛋白1(latent membrane protein 1,LMP1)基因被认为是EBV的致癌基因。近年的研究发现,LMP1基因的多态性尤其C末端30bp的缺失(del-LMP1)与其致瘤性密切相关,可能在肿瘤的发生发展中起着重要作用。本研究观察del-LMP1基因在非霍奇金淋巴瘤患者和健康人中的表达情况,以探讨缺失型LMP1基因是否与NHL的发生及临床预后相关。方法应用特异性引物,采用聚合酶链反应扩增NHL和健康人中LMP1基因片段(产物包括30bp缺失部分),根据扩增产物的大小判断其是否存在30bp的缺失,并随机抽取PCR产物进行测序。观察缺失型LMP1基因在两者之间的构成比,并分析缺失型LMP1基因与NHL临床预后的关系。结果(1)48例NHL中有23例LMP1基因片段扩增阳性,其中携带缺失型者18例(78%),原型5例(22%);60例健康人有32例LMP1基因片段扩增阳性,其中携带缺失型者13例(41%),原型19例(59%);NHL患者和健康人中30bp缺失型LMP1基因的表达有显著性差异(P<0.05)。(2)LMP1基因片断扩增阳性的NHL中,IPI≥3者为15例,其中缺失型14例(93%),原型1例(7%);IPI<3者为8例,其中缺失型4例(50%),原型4例(50%),缺失型LMP1在不同IPI值的NHL     

A Collaborative Nationwide Lymphoma Study in Lebanon: Incidence of Various Subtypes and Analysis of Associations with Viruses

Incidence of various Hodgkin (HL) and non-Hodgkin lymphoma (NHL) subtypes and association with viruses in Lebanon are not known. We undertook a nationwide study of 272 patients diagnosed with lymphoma in 2007. HL comprised 32.7 % (n?=?89) of cases while NHL represented 67.3 % (n?=?183). Consistent with the literature, nodular sclerosis was the most predominant HL subtype (n?=?57/89). Among NHL, B-cell NHL represented 88 % (n?=?161/183), T-cell NHL 9 % (n?=?17/183), whereas in 2.7 % it was not classifiable. The B-cell NHL comprised predominantly diffuse large B-cell lymphoma (46 %) and follicular lymphoma (23 %). 81 cases were reviewed by a panel of pathologists with 87.6 % concordance rate. Serology was negative for hepatitis C in 122 tested cases. HIV was positive in 2 cases. Two adult T-cell leukemia/lymphoma were HTLV-I positive. EBV IgG were positive in 88.5 % of cases. 38 EBV seropositive cases [27 NHL (24 B-cell, 3 T-cell) and 11 HL] were studied for EBV genome expression using EBV-encoded RNA (EBER)-in situ hybridization. EBER expression was positive in 8 (21 %) cases (6 HL, 2 T-cell NHL). The distribution of lymphoma subtypes in Lebanon appears similar to that of Western countries. The high rate of EBV positivity in HL and T-cell lymphoma by EBER deserves further investigation.     

非霍奇金淋巴瘤组织坏死相关因素的探讨

目的 探讨非霍奇金淋巴瘤组织坏死相关因素.方法 将124例NHL分成有坏死组和无坏死组两组;分成NK/T、TCL、BcL三种类型,用IHC(SP法)标记CD95(Fas)、CD95L(FasL)、bcl-2、TNF-α;ISH技术检测EB病毒编码的小核苷酸RNA(EBER1/2);病例随访.结果 坏死组Fas、FasL、EBER1/2的表达率依次为81.5%(22/27)、74.1%(20/27)、82.5%(23/27),无坏死组依次为68.1%(66/97)、59.8%(58/97)、12.4%(12/97),与无坏死组比较P<0.005,bcl-2表达正好相反,与坏死组比较P<0.005.Fas、FasL、EBER1/2表达主要集中在NK/T和TCL,与BCL比较差异有统计学意义;而bcl-2表达主要集中在BCL,与TCL及NK/T比较差异有统计学意义.坏死组、无坏死组平均生存时间分别为7.4月、24.2月,两组比较P<0.005.结论 Fas、FasL、bcl-2的异常表迭和EBV感染,在NHL的坏死中可能发挥了一定的作用,NHL中组织坏死是多因素共同作用的结果,组织坏死与预后有关.     

An autopsy case of aggressive CD30+ extra-nodal NK/T-cell lymphoma initially manifested with granulomatous myositis

This study reports an autopsy case of a 53 year-old male with rapidly progressive extra-nodal NK/T-cell lymphoma accompanied with unusual clinical and pathologic features. He was initially presented with localized swelling and tenderness in the right lower extremity and the biopsy from the calf muscle was interpreted as granulomatous myositis masquerizing lymphoma. The biopsy from erythematous skin lesion of trunk showed infiltration of medium sized atypical lymphoid cells with relatively plump cytoplasm and immunophenotype of CD30+, CD56+/- and surface CD3-, which lead to the diagnosis of CD30+ anaplastic large cell lymphoma. About 2 months later, nasal obstruction was developed and the nasal biopsy was done. After confirmation of EBV infection, he was finally diagnosed as extra-nodal NK/T-cell lymphoma with peculiar immunophenotype of CD3 dim+ and CD30+. Despite the chemotherapy, he was going rapidly downhill and died of respiratory and multi-organ failure 8 months after the onset of soft tissue lesion. At autopsy, disseminated angiocentric lymphoma was found all over the internal organs including the brain. This case emphasizes that extra-nodal NK/T-cell lymphoma should be considered as a cause of granulomatous myositis and can express CD30 positivity and CD3 weak positivity, which are unusual but rarely predominant feature of NK/T-cell lymphoma.     

恶性淋巴瘤患者外周血T淋巴细胞亚群变化的讨论

目的探讨恶性淋巴瘤患者T淋巴细胞亚群的变化特点。方法采用流式细胞术检测24例各型恶性淋巴瘤患者及20例正常人的外周血中T淋巴细胞(CD3+细胞)、辅助性T细胞(CD4+)、抑制性细胞毒T细胞(CD3+/CD8+)、NK细胞(CD3-/CD16+56)和NKT细胞(CD3+/CD16+56),对两者百分比进行比较分析。结果患者组中辅助性T细胞(CD3+/CD4+/CD8-)的百分比和绝对值明显低于正常对照组(P0.01),而患者组中CD4/CD8细胞亦明显低于正常对照组(P0.01)。结论在恶性淋巴瘤发生发展的过程中了解淋巴细胞亚群的变化,可为后期病情判断及可能采取的相关治疗提供依据。