PTEN、COX-2、Survivin在胃癌中的表达及意义

目的 探讨第10染色体同源丢失性磷酸酶张力蛋白(PTEN)、环氧合酶-2(COX-2)、生存素(Survivin)与胃癌发生发展的关系及作用机制.方法 应用免疫组化S-P法检测80例胃癌组织、15例非胃癌胃黏膜组织中PTEN、COX-2、Survivin的表达.结果 80例胃癌组织中42例PTEN蛋白表达明显下降或缺失,且与胃癌TNM分期、淋巴结转移相关(P＜0.05).COX-2在胃癌组织中的阳性率为67.5%,显著高于正常胃黏膜组织(P＜0.05),并相关于胃癌淋巴结转移、TNM分期.Survivin在胃癌组织中的阳性率为70.0%(P＜0.05),和胃癌分化程度、淋巴结转移、TNM分期有相关性(P＜0.05).在PTEN蛋白阴性的胃癌组中,COX-2阳性率为70.37%,显著高于PTEN阳性组(P＜0.05).结论 抑癌基因PTEN在胃癌及胃癌发展过程中表达逐渐下降或缺失,与凋亡抑制基因COX-2可能存在相互作用,协同Survivin参与了胃癌的发展、转移及浸润.     

Runx3在不同类型胃息肉与胃癌中的蛋白表达及与幽门螺杆菌感染的相关性

目的:检测不同病理类型胃息肉和胃癌中Runx3蛋白的表达和幽门螺杆菌(H.pylori)的感染率,探讨其在胃息肉与胃癌中的相关性.方法:对炎性胃息肉组25例、增生性胃息肉组25例、腺瘤性息肉组25例、胃癌组30例采用SP染色法检测Runx3的表达水平,HE染色、甲苯胺蓝染色和Warthin-Starry(W-S)银染法检测H.pyloti的感染情况.结果:胃癌组Runx3蛋白的阳性表达率明显低于正常胃黏膜组、炎性胃息肉组和增生性胃息肉组,差异有统计学意义(X2=8.967、5.632、4.289,均P<0.05);腺瘤性息肉组明显低于正常胃黏膜组、炎性胃息肉组和增生性胃息肉组,差异有统计学意义(P<0.05).胃癌组低于腺瘤性息肉组,但差异无统计学意义.H.pylori的感染率在胃癌组与正常胃黏膜组相比H.pylori感染率有统计学意义(P<0.05).胃息肉组和胃癌组中H.pylori、Runx3的表达率之间存在着负性相关.结论:Runx3蛋白表达下调与H.pylori感染可能在胃癌的发生过程中起着协同作用.     

幽门螺杆菌感染对老年人胃黏膜环氧合酶-2表达的影响及意义

目的:评估幽门螺杆菌(H pylori)感染对老年人胃黏膜COX-2表达的影响及意义.方法:取不同阶段的胃黏膜病变共200例,用速尿素酶试验结合组织学Giemsa染色或14C尿素呼气试验检测胃黏膜H pylori感染状况,应用免疫组织化学检测胃黏膜上皮细胞COX-2的表达.结果:不同组织类型H pylori检出率以胃癌最高,其次为不典型增生(AH)和肠上皮化生(IM).COX-2在慢性浅表性胃炎(CSG)、IM、AH和胃癌中的表达阳性率分别为8%、24%、46%和64%,呈递增趋势,其阳性率胃癌与非癌组织相比差异均有统计学意义(P<0.01).同一类型 H pylori 阳性组COX-2的表达高于H pylori 阴性组,2组比较有显著性差异(P＜0.05).结论:COX-2表达上调与H pylori感染的胃黏膜的癌变有关,可能在癌前病变早期阶段起作用.     

胃癌及癌前病变组织中PTEN蛋白的表达及意义

采用免疫组化ABC法对10例正常胃黏膜、16例胃黏膜轻度异型增生、12例中度异型增生、14例重度异型增生、16例早期胃癌及52例进展期胃癌组织中的PTEN蛋白进行了检测.结果显示,正常胃黏膜PTEN蛋白阳性表达率为90.0%,轻、中、重度异型增生胃黏膜分别为81.3%、75.0%、42.9%,早期及进展期胃癌分别为37.5%和36.5%.PTEN蛋白在轻度异型增生胃黏膜中的阳性表达率与重度异型增生、早期及进展期胃癌相比,P均<0.05;中度异型增生与早期及进展期胃癌相比,P均<0.05;肠型胃癌的阳性率(50.0%)与弥漫型胃癌(25.0%)相比,P<0.05;淋巴结转移胃癌的阳性率(14.8%)与未转移者( 51.2%)相比,P<0.05.认为PTEN蛋白在胃黏膜癌变过程中发挥了一定作用,可作为判断胃癌预后的一个有用指标.     

COX-2在胃溃疡与胃癌的表达及其与幽门螺杆菌的关系

[目的]探讨COX-2在幽门螺杆菌(Hp)感染和非感染胃溃疡与胃癌的表达。[方法]选择胃病患者共285例,分为胃溃疡患者(胃溃疡组)200例(病理分型:肠上皮化生96例和异型增生104例),胃癌患者(胃癌组)85例;以正常胃黏膜者50例为对照组。根据胃镜检查和组织病理学检查受试者胃黏膜中COX-2蛋白的表达,并比较各病理分型及Hp感染与非感染者COX-2蛋白表达的差异。[结果]COX-2蛋白在胃溃疡组的肠上皮化生、异型增生中及胃癌组癌细胞中均有表达,在正常胃黏膜组织中不表达。胃溃疡组和胃癌组的COX-2阳性表达均强于对照组,差异有统计学意义(P0.05);胃癌组的COX-2阳性表达强于胃溃疡组,差异有统计学意义(P0.05);胃溃疡组异型增生者COX-2阳性表达强于肠上皮化生者,差异有统计学意义(P0.05);胃癌组Hp阳性COX-2阳性表达强于胃溃疡组Hp阳性者,差异有统计学意义(P0.05);胃癌组Hp阴性者COX-2阳性表达强于胃溃疡组Hp阴性者,差异有统计学意义(P0.05);胃溃疡组和胃癌组中Hp阳性者COX-2阳性表达均强于Hp阴性者,差异有统计学意义(P0.05)。[结论]Hp感染会促进胃溃疡COX-2的表达,Hp感染和COX-2过度表达会使胃溃疡患者癌变的概率大大增加。     

MMP-7与Fas蛋白在胃癌组织中的表达及意义

目的:探讨基质金属蛋白酶-7(MMP-7)蛋白和Fas蛋白在胃癌组织中的表达、相互关系及意义.方法:应用免疫组化方法检测了82例胃癌组织及30例周边正常胃黏膜中MMP-7和Fas的表达情况.结果:胃癌组织中MMP-7蛋白阳性率显著高于正常胃黏膜(73.2%vs10%,P<0.001);正常胃黏膜中Fas蛋白阳性率显著高于胃癌组织(39.1%vs93.3%,P<0.001).MMP-7阳性表达率与淋巴结转移、TNM分期显著相关(P<0.001),而与肿瘤细胞分化程度无显著性相关.Fas蛋白阳性表达率与肿瘤细胞分化程度显著相关(P<0.05),而与淋巴结转移、TNM分期无显著性相关.胃癌组织中MMP-7与Fas表达具有显著等级负相关(r=-0.597,P<0.001).结论:MMP-7与Fas表达胃癌的生物学行为密切相关,且两者之间的表达强度具有显著等级负相关.     

EMMPRIN、HER-2蛋白表达与胃癌侵袭转移的关系

目的: 探讨细胞外基质金属蛋白酶诱导因子(EMMPRIN)、人表皮生长因子受体2(HER-2/neu)蛋白表达与人胃癌侵袭转移的关系, 以及2种蛋白表达之间的关系.方法: 应用量子点免疫荧光组织化学技术检测人胃癌组织芯片150芯(包括70例胃癌组织和5例正常胃黏膜组织)中EMMPRIN和HER2的蛋白表达, 并分析他们与临床病理特征的相关性.结果: 胃癌和正常胃黏膜组织中的EMMPRIN阳性表达率分别为72.86%和20.00%, 差异有显著性( P = 0.029). 在胃癌组织中HER-2蛋白的阳性率为47.14%(33/70), 高于正常胃黏膜组织, 但差异无显著性( P = 0.063).EMMPRIN、HER-2蛋白表达与胃癌患者年龄、性别、分化程度、肿瘤的浸润深度以及临床TNM分期之间差异均无显著性( P>0.05),仅与淋巴结转移显著相关( P<0.05). 在70例胃癌组织中EMMPRIN与HER-2蛋白表达之间呈显著正相关( r = 0.383, P = 0.001).结论: EMMPRIN与HER-2/neu可能协同促进了胃癌的淋巴结转移.     

胃癌及癌前病变中P27和Cyclin E蛋白的表达意义

目的:探讨P27和CyclinE蛋白在胃癌及癌前病变中的表达及其与胃癌病理参数之间的关系.方法:用免疫组化技术(SP法)对正常胃黏膜(normalgastricmucosa,NGM)、慢性浅表性胃炎(chronicsuperficialgastritisCSG)、慢性萎缩性胃炎(chronicatrophiagastritis,CAG)伴肠上皮化生、慢性萎缩性胃炎伴非典型性增生各20例和胃癌(gastriccarcinoma,GC)60例标本进行免疫组织化学染色,分析P27和CyclinE蛋白表达及其与胃癌临床和病理的关系.结果:各组胃组织中P27和CyclinE蛋白表达阳性率分别为NGM组100%和5%,CSG组85%和10%,CAG伴肠化组70%和20%,CAG伴不典型增生组45%和30%,胃癌组38.3%和40%.胃癌组和CAG伴不典型增生组P27阳性率显著低于其他组(P<0.05),CyclinE阳性率则显著高于其他组(P<0.05).P27和CyclinE在胃癌中的表达分别与肿瘤分化程度、浸润深度及肿瘤临床分期相关,P27蛋白的表达尚与有无淋巴结转移相关.P27和CyclinE蛋白在胃癌中的表达呈显著负相关(r=-0.768,P<0.05).结论:检测胃癌组织中P27和CyclinE蛋白的表达有助于判断肿瘤的进展程度,两者联合检测有助于判断肿瘤预后.     

胃癌和胃癌前病变中环氧合酶-2、p16的表达及其临床意义

背景：密切监测胃癌前病变是预防和及早发现胃癌的关键。目的：观察胃癌及其癌前病变中环氧合酶-2（COX-2）、p16的表达,探讨两者对胃癌早期诊断的意义。方法：以免疫组化染色检测20例正常胃黏膜、60例肠化生、60例上皮内瘤变和60例胃癌组织中COX-2和p16的表达,并分析两者的相关性。结果：正常胃黏膜、肠化生、上皮内瘤变和胃癌组织中的COX-2表达呈递增趋势,p16表达呈递减趋势。高级别上皮内瘤变和早期、进展期胃癌组织COX-2、p16表达阳性率与正常胃黏膜和肠化生组织相比差异有统计学意义（P〈0.05）,而前三者之间以及后两者之间无明显差异。COX-2、p16表达阳性率在小肠化生、完全型大肠化生与不完全型大肠化生之间以及早期与进展期胃癌之间无明显差异,但在高级别与低级别上皮内瘤变中差异有统计学意义（P〈0.05）。COX-2表达与p16表达呈负相关（P〈0.001）。早期胃癌组织中COX-2表达阳性同时p16表达阴性的比例显著高于高级别上皮内瘤变组织（P〈0.05）。结论：COX-2、p16或两者联合检测有助于监测和随访胃癌前病变、筛选胃癌高危人群,为胃癌的早期诊断提供了重要的检测方法。     

RASSF1A与CyclinD1在胃黏膜病变组织中表达及二者的关系

目的:研究RASSF1A蛋白和CyclinD1在胃癌及癌前病变组织中的表达及二者的关系.方法:收集延安大学附属医院2005-01/2006-10行手术切除的69例胃癌患者临床病理资料和切除标本的组织蜡块.同时收集慢性浅表性胃炎45例、慢性萎缩性胃炎45例、慢性胃炎伴肠化生45例及慢性胃炎伴不典型增生57例胃黏膜组织蜡块.采用免疫组化SP法检测胃癌及癌前病变胃黏膜组织中RASSF1A蛋白和CyclinD1的表达.结果:胃癌组织中RASSF1A表达的阳性率低于其在慢性浅表性胃炎胃黏膜组织中的阳性表达率(χ2=10.4,P＜0.05);RASSF1A蛋白的在胃癌癌前病变黏膜中阳性率表达逐渐降低(χ2=28.7,P＜0.05),并且与胃癌组织分化程度有关(P＜0.05).胃癌组织中CyclinD1表达的阳性率为84.1%,慢性浅表性胃炎黏膜组织中的几乎不表达:CyclinD1在胃癌癌前病变黏膜中阳性率表达逐渐升高(χ2=11.9,P＜0.05);胃癌组与其他各组之间阳性差率比较均存在显著性异(χ2=22.0,19.6,29.4,P＜0.01);RASSF1A蛋白和CyclinD1蛋白在胃癌中阳性表达率二者呈负相关(r=-0.323,P＜0.05).结论:胃黏膜在从慢性浅表性胃炎→慢性萎缩性胃炎→肠化生→不典型增生→胃癌这→演化模式过程中,RAssF1A蛋白表达减弱,CyclinD1蛋白表达增强;二者的联合检测,对研究胃癌发生发展机制有一定科学意义.     

Clinicopathological significance of LRP16 protein in 336 gastric carcinoma patients

AIM： To investigate the expression of leukemia related protein 16 （LRP16）, and the possible relationship between LRP16 expression and clinicopathological indices in 336 gastric carcinoma patients. METHODS： Immunohistochemistry was used to detect LRP16 expression in 336 cases of paraffin-embedded gastric carcinoma tissues and 60 cases of distal normal mucosa. The relationships between LRP16 expression and patients＇ age, tumor size, histological grade, clinical stage, metastatic status and prognosis were analysed. RESULTS： The expression of LRP16 was 58.6% （197/336） in gastric carcinoma and 31.7% （19/60） in distal normal gastric mucosa. The expression of LRP16 in carcinoma was significantly higher than that in normal mucosa tissues （x^2 = 14.929, P = 0.001）. LRP16 protein expression was found in 44.1% （63/143） carcinomas at stage Ⅰ and Ⅱ, and 69.4% （134/193） carcinomas at stage Ⅲ and Ⅳ （Z2 = 21.804, P = 0.001）, and in 56.9% （182/320） of cancers without metastasis but 93.8% （15/16） of those with metastasis （2 = 8.543, P = 0.003）. The expression of LRP16 was correlated with tumor size, infiltrative depth, clinical stage, lymphatic invasion and distant metastasis （all P 〈 0.05）. Follow-up data showed that there was a significant difference in median survival time between cancer patients with expression of LRP16 （27.0 mo） and those without （48.0 mo, Log rank =31.644, P = 0.001）. CONCLUSION： The expression of LRP16 may be associated with invasion, metastasis and prognosis of gastric cancer.     

Mechanism and clinical significance of cyclooxygenase-2 expression in gastric cancer

AIM: To determine the correlation between methylation status of 5' CpG island of cyclooxygenase-2 (COX-2) gene and protein expression in gastric cancer tissues for distinguishing the molecular characters of gastric cancers. METHODS: Methylation status of 5' CpG island of COX-2 gene was studied by PCR amplification after HpaⅡ and Hha I restrictive enzyme digestion;COX-2 expression was evaluated by immunohistochemical method. RESULTS: Hpa Ⅱ and HhaI site were all methylated in 12 normal gastric mucosa tissues, whereas they were demethylated in 77.27% (34/44) and 84.09% (37/44) gastric cancer tissues,respectively.Expression of COX-2 was detected in 68.18% (30/44) gastric cancer tissues, but no expression was found in normal gastric mucosa tissues. In gastric cancer tissues, COX-2 expression was correlated significantly with HpaⅡ site demethylation (29/30 vs 5/14, P<0.001 and HhaI site demethylation (28/30 vs 9/14,P<0.05). CONCLUSION: The demethylation of 5' CpG island of gene is necessary for COX-2 expression in human gastric cancer. The expression status of COX-2 may provide theoretical basis for COX-2 targeting gastric cancer treatments.     

胃癌组织Maspin,uPA,MMP-7表达的意义

目的:观察胃癌及正常胃黏膜Maspin,uPA, MMP-7表达的意义．方法:应用免疫组化SP法检测胃管状腺癌30 例,胃印戒细胞癌30例,正常胃黏膜组织20例中Maspin,uPA,MMP-7的表达情况．结果:在胃管状腺癌中Maspin,uPA,MMP-7 阳性表达率分别为50％,70％和80％;胃印戒细胞癌中阳性表达率分别为46．7％,76．7％和 90％;正常胃黏膜组织中阳性表达率分别为 90％,35％和30％．Maspin的表达与浸润深度、淋巴结转移相关,而与肿块的大小和TNM分期无关．uPA和MMP-7的表达与浸润深度、淋巴结转移、TNM分期相关,而与肿块的大小无关．Maspin的表达与uPA和MMP-7的表达呈负相关(P=0．012,r=-0．322;P=0．008,r= -0．341);uPA的表达与MMP-7的表达呈正相关 (P=0．034,r=0．274)．结论:Maspin在胃癌中表达下调,uPA和 MMP-7在胃癌中过表达,他们在胃癌的浸润转移中起重要作用,可作为反应胃癌病理生物学行为的有效指标．     

Relationship between expression and distribution of cyclooxygenase-2 and bcl-2 in human gastric adenocarcinoma

AIM: To explore expression and distribution features of COX-2 and bcl-2 in human gastric adenocarcinoma tissues and to study its biological significance. METHODS: Totally 36 human gastric carcinoma samples were enrolled in this study (cardiac adenocarcinoma 16 cases, distal gastric adenocarcinoma 20 cases). The expressions of COX-2 and bcl-2 in cancerous tissues and corresponding para-cancerous tissues were investigated by immunohistochemistry using COX-2 polyclonal antibody and bcl-2 monoclonal antibody. The normal gastric mucosa tissues were used as control. RESULTS: The expressions of COX-2 and bcl-2 in gastric carcinoma were significantly higher than that in the para-cancerous tissues (77.8% vs 47.2%, P<0.01, 80.56% vs 58.33%, P<0.05). The expression of COX-2 in cardiac adenocarcinoma was remarkably higher than that in the distal gastric carcinoma (93.8% vs 65.0%, P<0.01). The expression of COX-2 was mainly localized in the cytoplasm of tumor cells and partly in the nucleus. There is a transition of the COX-2 cytoplasmic positivity to nucleic in tumor cells with the increase of gastric carcinoma pathological grade. Interstitial macrophages, fibroblasts and vascular endothelial cells also expressed COX-2. The tissues with higher expression of COX-2 also expressed high level of bcl-2 protein. CONCLUSION: Abnormal expression pattern of COX-2 within the tissues of human gastric cancer is correlated with tumor location and lymph node metastasis. COX-2 may regulate expression of apoptosis suppressor gene (bcl-2) through interaction of tumor cells and stromal cells and play an important role in the generation and development of tumors, which will be of great help in developing new methods for antitumor therapy.     

Expression of ornithine decarboxylase in precancerous and cancerous gastric lesions

AIM: To investigate the expression of ornithine decarboxylase (ODC) in precancerous and cancerous gastric lesions. METHODS: We studied the expression of ODC in gastric mucosa from patients with chronic superficial gastritis (CSG,n = 32),chronic atrophic gastritis CAG,n = 43; 15 with and 28 without intestinal metaplasia (IM),gastric dysplasia (DYS,n = 11) and gastric cancer (GC,n = 48) tissues using immunohistochemical staining. All 134 biopsy specimens of gastric mucosa were collected by gastroscopy. METHODS: The positive rate of ODC expression was 34.4%,42.9%,73.3%,81.8% and 91.7% in cases with CSG,CAG without IM,CAG with IM,DYS and GC,respectively (P < 0.01),The positive rate of ODC expression increased in the order of CSG < CAG (without IM) < CAG (with IM) < DYS and finally,GC. In addition,ODC positive immunostaining rate was lower in well-differentiated GC than in poorly-differentiated GC (P < 0.05). CONCLUSION: The expression of ODC is positively correlated with the degree of malignity of gastric mucosa and development of gastric lesions. This finding indicates that ODC may be used as a good biomarker in the screening and diagnosis of precancerous lesions.     

COX-2 expression in gastric cancer and its relationship with angiogenesis using tissue microarray

AIM： To explore the expression and clinicopathological significance of cyclooxygenase-2 （COX-2） and microvessel density （MVD） in gastric carcinogenesis, and to investigate their roles in the invasion and the relationship between biological behaviors and prognosis of gastric cancer.
METHODS： Using Envision immunohistochemistry, COX-2 and CD34 expressions in gastric cancer tissue array were examined. MVD was counted and the relationship between the biological behaviors and prognosis was analyzed.
RESULTS： The expression of COX-2 in gastric cancer tissue was significantly higher than that in normal mucosa （χ^2 = 12.191, P 〈 0.05）. The over-expression of COX-2 in gastric cancer was obviously related to metastasis and depth of invasion （χ^2 = 6.315, P 〈 0.05）, but not related to the histological type and Borrmann type （χ^2 = 5.391 and χ^2= 2.228, respectively）. Moreover, MVD in gastric cancer tissues was significantly higher than that in the normal mucosa （65.49 ± 20.64 vs 36.21 ± 18.47, t/F = 7.53, P 〈 0. 05）. MVD was related to the histologic type and metastasis （t/F= 3.68 and t/F = 4.214, respectively, P 〈 0. 05）, but not related to the depth of invasion and Borrmann type （t/F = 0.583 and t/F = 0.459, respectively）. MVD in COX-2-positive tissues was markedly higher compared to COX-2-negative tissues, indicating a positive correlation between COX-2 expression and MVD （t = 13.12, P 〈 0. 05）.
CONCLUSION： Tissue microarray （TMA） is a powerful tool for rapid identification of the molecular alterations in gastric cancer. COX-2 expression, via inducingangiogenesis, may play an important role in gastric carcinogenesis. It could be served as a determinant factor for clinical prognosis and curative effect.     

Increased expression of cyclooxygenase-2 in first-degree relatives of gastric cancer patients

AIM: To study the expression of cydooxygenase-2 (COX-2) in human gastric cancer tissues and their paired adjacent mucosa, as well as mucosa from gastric antrum and corpus of the first-degree relatives of the recruited cancer patients. METHODS: The expression of COX-2 mRNA in 38 patients with gastric cancer and their 29 first-degree relatives and 18 healthy controls was assessed by the real time RT-PCR. The expression of COX-2 protein was determined by Western blot. RESULTS: A marked increase in COX-2 mRNA expression was found in 20 of 37 (54%) cancerous tissues compared to their respective paired normal mucosa (P<0.001). Interestingly, increased COX-2 mRNA expression was also found in mucosa of the corpus (6/29) and antrum (13/29) of their first-degree relatives. Increased COX-2 mRNA expression was more frequently observed in the antrum biopsies from cancer patients than in the antrum biopsies from healthy controls (P<0.05). In addition, 3 of 23 (13%) patients with atrophic mucosa and 6 of 35 (17%) patients with intestinal metaplasia showed increased COX-2 mRNA expression. Furthermore, COX-2 expression increased in H pylori-positive tissues, especially in antrum mucosa. CONCLUSION: Increased COX-2 expression is involved in gastric carcinogenesis, and may be necessary for maintenance of the malignant phenotype and contribute to Helicobacter pylori-associated malignant transformation.     

Relationship between cell adhesion molecules expression and the biological behavior of gastric carcinoma

AIM： To evaluate the relationship between the expression of cell adhesion molecules （CAMs） and the biological behavior of gastric carcinoma. METHODS： Expression of syndecan-1, E-cadherin and integrin β3 were evaluated by immunohistochemical study in a total of 118 gastric carcinomas and 20 nontumor gastric mucosas. RESULTS： The expressions of syndecan-1 and E-cadherin were significantly lower in gastric carcinoma compared to non-tumor gastric mucosa, and the low expression rates were positively correlated to the tumor invasion depth, vessel invasion, lymph node metastasis and distant metastasis （P 〈 0.01 in all cases）. However, the expression of integrin β3 was significantly higher in gastric carcinoma compared to non-tumor gastric mucosa, and the high expression rates were positively correlated to the tumor invasion depth, vessel invasion, lymph node metastasis and distant metastasis （P 〈 0.01 in all cases）. In addition, the three protein expressions were correlated to the tumor growth pattern （P 〈 0.01, P 〈 0.01, and P 〈 0.05 respectively）, but not correlated to tumor differentiation （P 〉 0.05, P 〉 0.05 and P 〉 0.05 respectively）. Positive correlation was observed between the expressions of syndecan-1 and E-cadherin, but they which were negatively correlated to the expression of integrin β3 （P 〈 0.01 in all cases）. Univariate analysis demonstrated that the mean survival time and 5-year survival rate were lower in the cases with low expressions of syndecan-1 and E-cadherin and high expression of integrin β3 （P 〈 0.01, in all cases）. COX multivariate analysis showed that the expression level of syndecan-1 could be an independent prognostic index of gastric carcinoma （P 〈 0.01）, whereas E-cadherin and integrin β3 could not be independent indexes （P 〉 0.05, P 〉 0.05 respectively）.CONCLUSION： The low expression of syndecan-1 and E-cadherin and the high expression of integrin β3 are significantly correlated with the invasion and metastasis o     

Ezrin和HER2在胃癌组织芯片中的表达及意义

目的:探讨Ezrin蛋白在胃癌组织中的表达,与肿瘤浸润、转移的关系及与HER2的相互作用.方法:485例原发性胃癌组织中高、中、低分化胃癌分别为19例、235例和231例;有淋巴结转移者353例;TNM分期Ⅰ、Ⅱ期166例,Ⅲ、Ⅳ期319例.另外取距肿瘤7cm的正常胃黏膜组织40例.制成8个组织芯片蜡块,用免疫组织化学方法检测石蜡包埋的胃及胃癌组织中的Ezrin和人类表皮生长因子受体2(hum an epidermal growth factor receptor 2,HER2)蛋白表达.所有患者均经外科手术治疗,病理诊断明确,术前未经放、化疗.结果:Ezrin和HER2在胃癌组织中高表达,二者均与肿瘤Lauren’s分型和肿瘤分化程度相关(χ2=17.625,χ2=20.386,均P=0.000;χ2=9.474,P=0.009,χ2=13.377,P=0.010);Ezrin同时还与组织学(日本分型)、TNM分期、浸润深度和淋巴结转移相关(χ2=37.542,P=0.000;χ2=12.237,P=0.002;χ2=21.194,P=0.002;χ2=9.868,P=0.007).Ezrin和HER2蛋白表达呈正相关(r=0.129,P=0.004).结论:Ezrin可能是预测胃癌组织浸润、转移有用的指标;联合检测Ezrin和HER2可作为判断胃癌预后、筛选高危转移患者的有效指标并有可能用于指导胃癌的个体化治疗.     

慢性萎缩性胃炎黏膜上皮中P53和C-erbB-2表达的临床意义

目的:探讨慢性萎缩性胃炎(CAG)黏膜上皮中P53及C-erbB-2的表达及意义．方法:用免疫组化技术(SP法)检测正常胃黏膜56例、慢性萎缩性胃炎429例(腺体囊性扩张61例,大肠型化生73例,轻、中、重度不典型增生各120、91和84例)和早期胃癌57例中P53和C-erbB-2的表达,分析P53和C-erbB-2表达及其与CAG胃黏膜病变类型的关系．结果:正常胃黏膜,囊性扩张腺体,轻、中度不典型增生,大肠型化生,重度不典型增生,早期胃癌P53和C-erbB-2表达的阳性率呈上升趋势．前三组间表达率差异无统计学意义(P>0．05);正常胃黏膜与中度不典型增生、大肠型化生、重度不典型增生及胃癌组P53和C-erbB-2的表达差异有统计学意义(P<0．01)．Spearman等级相关分析显示P53和C-efbB-2表达呈正相关(r=0．867,P<0．05)．年龄<40岁和≥40岁组间、性别组间P53表达阳性率差异有统计学意义(x2=12．393,P<0．01;x2=8．799, P<0．01)．C-erbB-2的表达在上述年龄组间差异有统计学意义(x2=7．706,P<0．01),而在性别组间无统计学意义(P>0．05)．结论:检测慢性萎缩性胃炎中P53和C-erbB-2的表达,有助于监测CAG癌前病变的进展及胃癌的早期发现．