增殖细胞核抗原和p53蛋白在涎腺肿瘤中的表达

为了研究增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）和ｐ５３在涎腺肿瘤中的表达，作者用抗ＰＣＮＡ及ｐ５３单克隆抗体对良、恶性混合瘤及粘液表皮样癌组织标本进行了免疫组化染色。结果表明，恶性混合瘤ＰＣＮＡ增殖指数较高，与混合瘤细胞生长活跃型及混合瘤相比差异有极显著性（Ｐ＜０．０１）；低分化粘液表皮样癌的ＰＣＮＡ阳性表达明显高于高分化型（Ｐ＜０．０１）。ｐ５３在恶性混合瘤中阳性表达率高（６０．０％），与粘液表皮样癌（２０．０％）相比差异有显著性（Ｐ＜０．０５）。另外，我们观察到ｐ５３阳性表达的肿瘤组织ＰＣＮＡ增殖指数较高。结论：ＰＣＮＡ增殖指数可以作为支持诊断恶性混合瘤的指标；是粘液表皮样癌组织学分级的重要参数。ｐ５３蛋白是恶性混合瘤的有效标记物；ｐ５３蛋白参与ＰＣＮＡ表达的调节。     

P53蛋白在恶性混合瘤和粘液表皮样癌中的表达和意义

本研究应用抗Ｐ５３蛋白单克隆抗体ＤＯ－１对恶性混瘤和粘液表皮样癌进行免疫组织化学染色，评价抑癌基因Ｐ５３在恶性混合瘤和粘液表面样癌中的作用，结果表明：Ｐ５３在性混合瘤中有较高的阳性表达率（６０．０％）与粘液表皮样癌（２０．０％）相比有显著性差异（Ｐ＜０．０５），且前者的阳性表达强度明显高于后者。因此认为，Ｐ５３抑癌基因的突变是恶性混合瘤发生的重要机制之一，Ｐ５３蛋白的阳性表达可以作为恶性混合瘤的一     

增殖细胞核抗原及P53在舌癌中的表达

目的：研究ＰＣＮＡ、Ｐ５３在舌癌中表达的相关性及与肿瘤病理分级、淋巴结转移、预后的关系。方法：应用免疫组化ＳＰ法，观察４６例病理确诊的舌癌石蜡标本中ＰＣＮＡ指数及Ｐ５３阳性率的表达。结果：ＰＣＮＡ在所有病例均有不同程度表达，４６例中２８例呈Ｐ５３阳性表达（６０．９％）。Ｐ５３阳性组中ＰＣＮＡ指数明显高于Ｐ５３阴性组（Ｐ＜０．００１）。ＰＣＮＡ指数随舌癌病理分级的升高而增加（Ｐ＜０．０２５）。Ｐ５３阳性率在Ⅱ、Ⅲ级舌癌明显高于Ⅰ级舌癌（Ｐ＜０．０５）。在有淋巴结转移及术后生存小于３年组中ＰＣＮＡ指数及Ｐ５３阳性率均明显高于无淋巴结转移及术后生存３年以上组（Ｐ＜０．００１，Ｐ＜０．０５）。结论：联合检测ＰＣＮＡ及Ｐ５３对判断舌癌恶性度，预测淋巴结转移趋势和预后及指导临床治疗有重要价值。     

涎腺粘液表皮样癌中P53基因表达的研究

目的 探讨Ｐ５３基因在涎腺粘液表皮样癌发生中的作用和意义。方法 采用免疫组织化学方法对４０例涎腺粘液表皮样癌中的表达进行观察。结果 低分化粘液表皮样癌过度表达Ｐ５３基因,阳性表达率为４２．１％,高分化粘液表皮样癌不表达Ｐ５３。经统计学分析表明,高、低分化肿瘤之间差异有显著性。结论 高、低分化的粘液表皮样癌发生时在癌基因激活方面存在差异。Ｐ５３基因突变可能在低分化粘液表皮样癌的发生中具有重要意义。Ｐ     

增殖细胞核抗原PCNA在涎腺瘤中的表达

采用抗增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇ ｃｅｌｌ ｎｕｃｌｅｒ ａｎｔｉｇｅｒ，ＰＣＮＡ）单克隆抗体ＰＣ１０，对４９你涎腺瘤标本进行免疫组化染色。结果显示：涎腺恶性肿瘤与正常涎腺组织和良性肿瘤间，离分化与低分化粘度表皮样癌间及腺样囊性癌实质型与筛孔型之间ＰＣＮＡ标记指数均存在显著差异。提示：ＰＣＮＡ表达对判断涎腺肿瘤的增殖活性有一定意义。     

口腔鳞状细胞癌增殖细胞核抗原的研究

作者采用抗增殖细胞核抗原单克隆抗体，对５２例标本进行了免疫组化染色。结果显示：正常口腔粘膜基底细胞具有少量的ＰＣＮＡ阳性细胞，其阳性分级为１级；而Ｉ级鳞癌ＰＣＮＡ阳性分级为３级为主，随着鳞癌组织学分级的升高，ＰＣＮＡ阳性分级有增高趋势，ＩＩＩ级鳞癌的ＰＣＮＡ阳性分级以４级为主，经统计学分析：除鳞癌Ｉ级与ＩＩ级间无显著差异外（Ｐ＞０．０５），其余各组间均有显著差异（Ｐ＜０．０５）。提示：ＰＣＮＡ表达     

p53、C-erbB-2癌基因蛋白在涎腺粘液表皮样癌中的表达及其临床病理学意义

目的探讨癌基因蛋白p53、C-erbB-2（p185）过度表达与粘液表皮样癌生物学行为的关系。方法利用微波免疫组织化学方法对32例人粘液表皮样癌的癌基因蛋白p53、C-erbB-2进行检测。结果正常诞腺组织p53、C-erbB-2均为阴性反应。癌旁导管上皮细胞两者阳性率分别为10.0％及15.0％,但腺泡细胞阴性。粘波表皮样癌组织中p53、C-erbB-2阳性率分别为40.6％及46.9％。p53、C-erbB-2在粘液细胞、表皮样细胞及中间细胞内均有表达。p53、C-erbB-2的表达与粘液表皮样癌肿瘤组织学类型、分化程度及肿瘤复发等肿瘤生物学行为密切相关（P＜0.01）。结论p53、C-erbB-2可作为粘液表皮样癌的分化性标志物,用于监测病情、判断预后等。     

口腔基底样鳞状细胞癌的临床病理及免疫组织化学特点

目的研究口腔基底样鳞状细胞癌的临床病理学特点。方法用ＨＥ染色、病理学观察及免疫组织化学染色法对１４例基底样鳞状细胞癌进行了分析。结果口腔基底样鳞状细胞癌占口腔鳞状细胞癌的１％，男性多于女性，可发生在口腔粘膜任何部位，临床表现主要为肿物和溃疡。肿瘤由基底样细胞和鳞状细胞构成，以基底样细胞为主，常形成粉刺样坏死，核分裂较多，邻近上皮常有异常增生。肿瘤细胞角蛋白ＡＥ１／３、ＣＫ１３阳性，Ｓ１００阴性，增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）阳性细胞多，ｐ５３阳性率为５０％。３５．７％的病例初次手术时已有淋巴结转移。结论口腔基底样鳞状细胞癌较普通鳞状细胞癌恶性程度高，具有独特的组织学特点，临床及病理上应予重视。     

c-erbB-2癌基因在粘液表皮样癌中的表达

ｃ－ｅｒｂＢ－２癌基因在粘液表皮样癌中的表达王洁董福生王小玲尤红煜王永军我们采用ｃ－ｅｒｂＢ－２癌基因蛋白研究粘液表皮样癌，以探讨其在肿瘤中的过度表达及其在与腺癌鉴别诊断中的意义。１．材料与方法：从存档资料中挑选出涎腺粘液表皮样癌４２例（高分化２２例...     

口腔鳞癌细胞增殖核抗原和P53免疫组化的比较研究

本文运用ＡＢＣ免疫组化结合图象分析，定量分析了ＰＣＮＡ（细胞增殖核抗原）和Ｐ５３在口腔鳞癌中的表达。探讨了两者在口腔鳞癌的临床病理、肿瘤侵龚和转移等方面的异同点。结果表明：ＰＣＮＡ的表达强度和阳性率与肿瘤的病理分级有关，与肿瘤细胞的侵龚转换无关。而Ｐ５３和肿瘤细胞的侵龚有关密切的关系（Ｐ〈０．０５）。Ｐ５３阳性者，淋巴结转移率较高（８０％）。结果表明在口腔鳞癌的临床行为恶性，预后等方面定量分析上述     

Expression of p53 in oral mucosal hyperplasia, dysplasia and squamous cell carcinoma

OBJECTIVE: To assess p53 expression in a range of oral mucosal lesions and to relate the results to the clinical outcome in patients with dysplastic oral mucosal lesions and oral squamous cell carcinomas (OSCC).
MATERIALS AND METHODS: Archival tissue was available for eight cases of normal oral mucosa, 50 cases of oral mucosal hyperplasia, 41 cases of oral mucosal dysplasia and 48 cases of OSCC. The monoclonal antibody DO-7, reactive to p53 protein, was applied to paraffin-embedded sections using microwave pretreatment and immu-nohistochemical techniques.
RESULTS: The results showed that normal oral mucosa did not express p53.Positive nuclear staining was found in 18/50 (36%) cases of hyperplasia, 35/41 (85%) cases of dysplasia and 45/48 (94%) cases of OSCC.None of the p53 negative dysplasias progressed, while 19% of p53 positive cases of dysplasia recurred following excision and 11% of the cases underwent neoplastic transformation. Five out of 10 (50%) cases of severe dysplasia which were p53 positive resolved.
CONCLUSION: The proportion of cases with positive p53 expression increased from hyperplasia to dysplasia to OSCC. These results may indicate an involvement of p53 in neoplastic transformation as well as in proliferative events although the presence or absence of p53 staining could not be used to predict the outcome of potentially malignant oral mucosal lesions.     

Immunohistochemical analysis of the p53 tumor suppressor gene product in oral leukoplakia

Squamous Cell Carcinoma (SCC) is the most frequent malignancy in the oral cavity. p53 protein has been reported to be expressed at high levels in malignant lesions, while the level in premalignant lesions has yet to be determined. In this study, oral leukoplakia and oral SCC were examined. Seventy-four incision or excision samples from 43 cases diagnosed as leukoplakia, and 41 samples from 37 SCC cases in the oral cavity, were obtained. All samples (formalin-fixed, paraffin embedded) were examined immunohistochemically for overexpression of p53 protein with monoclonal antibody BP 53-12. As the result, 1. Twenty-two out of 43 leukoplakia cases, and 29 out of 37 oral SCC cases, were positive for p53 protein. 2. p53 protein was overexpressed in premalignant lesions, especially in the cases with moderate and severe epithelial dysplasia. 3. There was a relation between p53 protein expression and pathological features of leukoplakia (epithelial dysplasia), statistically. 4. There was a relation between p53 protein expression and clinical features of leukoplakia, statistically. 5. Malignant transformation during clinical observation was seen in 11 cases. Nine out of 11 cases were positive for p53 even before malignant transformation. Since in cancer-development cases, p53 staining was detected even before malignant transformation of oral leukoplakia to squamous cell carcinoma, it is indicated that p53 accumulation occurred at a early stage of cancer-development. In conclusion, immunohistochemical analysis of p53 protein is suggested to be useful diagnostic procedure for oral leukoplakia, which may develop into oral SCC.     

鼠双微粒体2蛋白、P53在口腔白斑和鳞癌中的表达及其相关性的研究

目的 研究鼠双微粒体2(MDM2)和P53在口腔白斑及鳞癌中的表达及其相关性.方法采用免疫组织化学SP方法对15例正常口腔黏膜组织、24例口腔白斑(OLK)组织及41例口腔鳞癌组织中的MDM2蛋白和P53蛋白进行检测.结果 正常黏膜中未见MDM2和P53阳性表达,口腔白斑和口腔鳞癌组织中MDM2阳性率分别为58.3%和...     

Alterations in expression of retinoid receptor beta and p53 in oral submucous fibrosis

OBJECTIVE: Knowledge of the molecular pathogenesis of oral submucous fibrosis (OSF), a potentially malignant condition with high risk of transition to oral cancer, is meagre. Alterations in the expression of retinoic acid receptor beta (RARbeta) and tumor suppressor gene, p53 are early events in oral tumorigenesis. The aim of this study was to investigate the alterations in the expression of RARbeta and p53 in OSF lesions and determine their association with disease pathogenesis. METHODS: The expression of RARbeta and p53 proteins was analyzed by immunohistochemistry in 50 cases of OSF and 30 histologically normal oral tissues. RESULTS: No detectable RARbeta expression was observed in 35 of 50 (70%) OSF cases. p53 protein accumulation was observed in 24 of 50 (48%) OSF cases analyzed. Thirty-six percent OSF lesions showed loss of RARbeta and p53 overexpression. Interestingly, 41 of 50 (82%) of OSF lesions showed altered expression of at least one of these two proteins. CONCLUSION: Altered expression of either RARbeta or p53 in majority of OSF lesions suggests their association with disease pathogenesis and warrants follow-up to determine whether OSF lesions harboring concomitant alterations in RARbeta and p53 are at a high risk of transition to malignancy.     

Expression and mutations of p53 in salivary gland tumours

A series of 219 salivary gland tumours (103 carcinomas and 116 benign tumours) were analysed for p53 protein expression using immunohistochemistry, and for mutations in p53 gene using non-radioactive single strand conformation polymorphism (SSCP). p53 expression was present in 36% (42/116) of the benign tumours and in 54% (56/103) of the carcinomas. The highest prevalence of p53 expression was found in adenoid cystic carcinomas (69%). followed by mucoepidermoid carcinomas (67%). Of the benign tumours, pleomorphic adenomas showed the highest prevalence of p53 positivity (41%). In malignant tumours, expression of p53 bore no correlation to local recurrence, metastatic disease or survival of the patients. Exons 5 through 9 were analysed and four mutations were found in 20 cases of p53-immunopositive tumours and two in 20 p53-negative tumours. Each of the exons 5.6 and 8/9 had two mutations, whereas no mutations were detected in exon 7.     

血管化腓骨瓣修复涉及髁突下颌骨缺损的临床应用

目的:对累及髁突的不同类型下颌骨缺损采用游离腓骨瓣修复的方法、经验进行总结。方法:2001年10月-2004年10月,53例累及下颌升支上部的颌骨良性病损,行下颌骨切除后采用游离腓骨瓣进行下颌骨重建,分别采用了腓骨代替髁突法(30例)、游离髁突法(7例)及保留髁突法(16例)3种方法进行下颌骨缺损修复重建。分析总结临床方法经验,并对患者术后外形和功能进行临床评价。结果:本组血管化游离腓骨瓣移植成活率98.1%(52/53);41例患者术后随访,进行了临床检查及外形、功能评价,经Fisher检验,腓骨替代髁突组、游离髁突组及保留髁突组患者在术后外形和进食、语音功能上无显著差别。结论:3种不同髁突处理方法进行游离腓骨瓣下颌骨缺损重建,在临床上实用可行,可依据患者病损的具体情况选用。     

p53 expression in oral precancer and cancer

Expression of the p53 tumour suppressor gene is a frequent finding in human malignancies, including oral cancer, and it has been detected in some potentially malignant lesions. The results of the present project showed that 35 of the 41 (85 per cent) oral mucosal lesions with histological evidence of epithelial dysplasia expressed p53, but the presence or absence of p53 staining could not be used to predict the outcome of potentially malignant oral mucosal lesions.     

腭部骨折临床分型和治疗

目的探讨腭骨骨折的临床分型及治疗方法。方法通过回顾分析94例腭部骨折，根据临床表现并结合文献对腭骨骨折分为三型，分别对每一型腭部骨折进行治疗探讨。其中观察、保守治疗41例．手术切开复位内固定53例。结果本组资料中所有病例均一期愈合，咬合关系恢复正常．未出现术后植人物外露、口鼻瘘等并发症。结论采用不同形式的复位方法治疗不同类型的腭部骨折．能进一步恢复患者的面部外形及咬合功能，固定可靠。     

p53 aberrations in oral sub mucous fibrosis and oral cancer detected by immunohistochemistry.

Study of expression of p53 oncoprotein in several precancerous and cancer have been done, but only one literature is available regarding p53 expression in Oral Sub Mucous Fibrosis (OMSF), hence this study was taken up (i) to determine the expression of aberrant p53 in Oral Sub Mucous Fibrosis (OSMF) and Oral Squamous cell carcinoma (SCC) patients. (ii)To study correlation if any between p53 expression and degree of dysplasia in OSMF and SCC patients and (iii)To study correlation if any between p53 expression and habits in OSMF and SCC patients. Study Design consists of biopsy specimens of 38 cases of OSMF and 37 cases of Squamous cell carcinoma were subjected for staining by immunohistochemistry for p53 protein using LSAB visualization system kit. Clinical details along with habits were recorded and the data analyzed with t- test and chi- square test. Results of the study reveals 18 cases of OSMF and 26 cases of SCC were positive for p53 protein. Only 4 cases of SCC showed (++)grade and the rest all had (+)grade. Out of 75 patients, 65 had the habit of smoking and chewing, 4 patients history of habit was not known. Among patients with habits (65), 40 specimens were +ve for p53 stain and 2 out of 6 without history of habit, 2 out of 4 unknown history of habit took up p53 stain. To conclude study showed higher percentage of p53 positive cells in oral cancer cases when compared to oral sub mucous fibrosis cases. It suggests p53 expression may correlate with increase in dysplasia or malignant transformation. Both smoking and chewing habits had a significant role in p53 positive expression.     

Incidence and pattern of maxillofacial fractures in the elderly

The results of an audit analysing patients above 60 years and above treated for maxillofacial fractures over a 15-year period at the University College Hospital, lbadan are presented. Of the total number of 1689 patients of all ages treated for facial fractures, 53 (3.1%) were within the age range of the study.The male to female ratio was 1.1:1 and 41 (77.4%) patients were younger than 70 years. Road traffic accidents were the most common cause of facial fractures (58.5%) and the mandible was involved in 91% of cases. Complications were noted in 2 (3.8%) patients.Attention should be paid to update driving courses and home safety instructions in order to reduce the incidence of maxillofacial fractures in the elderly.