黄岑同源四倍体株系中黄岑苷含量的比较

黄岑为唇形科植物黄岑(Scutellaria baicalensis Georgi)的干燥根,研究对离体组织培养条件下诱导获得的黄岑四倍体株系,在进行田间农艺性状的观察比较的同时,用高效液相色谱测定了20个黄岑四倍体株系中黄岑苷的含量.结果表明在供试的20个黄岑四倍体株系中,有1个株系的黄岑苷含量高于二倍体,大部分与二倍体接近,但多倍体株系的根部产量明显大于二倍体,因此从大部分株系的有效成分黄岑苷生产量看,大多数四倍体明显优于二倍体.这一结果为今后进一步选育产量高、黄岑苷含量高的优良黄岑品种展示了较好的前景-     

天山雪莲大孔树脂提取物指纹图谱研究与3种活性成分含量测定

目的采用HPLC法建立天山雪莲大孔树脂提取物指纹图谱,并同时测定3种主要活性成分芦丁、紫丁香苷和绿原酸的含量。方法色谱柱为Kromasil C18柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(含0.8%醋酸,0.2%三乙胺),梯度洗脱;检测波长为270 nm;流速为1 mL·min-1;柱温为30℃,记录各批次天山雪莲大孔树脂提取物的相关数据并同时测定3种主要活性成分的含量。结果确立了天山雪莲大孔树脂提取物指纹图谱,并同时测定3种主要活性成分的含量。结论该方法精密度、稳定性和重复性良好,采用指纹图谱并结合主要活性成分含量测定可稳定控制天山雪莲大孔树脂提取物的质量。     

天山大黄总黄酮超声提取及含量测定

目的从天山大黄中提取总黄酮,并测定其含量。方法运用超声技术连续提取天山大黄总黄酮,用比色法测定总黄酮含量。结果测得天山大黄中总黄酮含量0.385%,平均回收率为98.96%,RSD=1.45%(n=6)。结论首次运用超声技术从天山大黄中连续提取总黄酮,速度加快,收率提高。     

雪莲降脂口服液的研制及质量控制

目的探索雪莲降脂口服液的制备工艺及定性定量方法,用以控制制剂的质量.方法采用TLC鉴定雪莲降脂口服液中的大黄素,以紫外分光光度法测定口服液中总黄酮的含量.结果雪莲降脂口服液中含有大黄素,所含总黄酮量为2.56 g/L,回收率为97.9%,标准差为0.0158.结论所建方法具有简便、直观,可操作性强的特点,可作为雪莲降脂口服液的质量控制.     

人工培植雪莲黄酮含量测定及其抗炎作用的研究

目的提取人工培植雪莲中的总黄酮,并研究其抗炎作用。方法采用醇提的方法获得雪莲总黄酮,以硝酸铝作为显示剂,以芦丁为对照品,利用分光光度计测定其波长在510nm处的吸光度值,测定其总黄酮的含量 采用角叉菜胶致足肿胀法和棉球致肉芽肿造成大鼠炎症动物模型,观察人工培植雪莲黄酮抗大鼠炎症的影响。结果人工培植的雪莲总黄酮提取含量达3.59%,同时该黄酮对炎症早期出现的渗出、水肿有明显的抑制作用。结论人工培植雪莲花的总黄酮对炎症大鼠模型有明显的抗炎症作用。     

天山雪莲提取物平衡溶解度和表观油水分配系数的测定

目的:测定天山雪莲提取物的平衡溶解度及表观油水分配系数。方法:采用HPLC法测定了天山雪莲提取物在水和7种有机溶剂中的平衡溶解度,及天山雪莲提取物在正辛醇-水/缓冲盐溶液中的表观油水分配系数。结果:分别以绿原酸和芦丁为指标,天山雪莲提取物在水中的平衡溶解度为400.81mg.L-1和559.65mg.L-1,表观油水分配系数为0.57(logPapp=-0.24)和0.61(logPapp=-0.22),甲醇中溶解度较大,分别为974.73mg.L-1和2 413.64mg.L-1。结论:天山雪莲提取物的脂溶性较好,其平衡溶解度和油水分配系数受pH的影响较大,为天山雪莲透皮给药制剂的研究提供基础。     

紫外-可见分光光度法测定细毡毛忍冬中总黄酮的含量

目的:建立一种简单、快速的方法,测定细毡毛忍冬中总黄酮含量,为细毡毛忍冬的质量控制和开发利用提供科学依据.方法:以芦丁为对照品,采用紫外分光光度法,在波长510nm处测定细毡毛忍冬总黄酮含量.结果:芦丁对照品溶液在8.0179～48.1074μg.mL-1浓度范围内,吸光度与浓度呈良好线性关系,细毡毛忍冬中总黄酮含量为3.38％.结论:该测定方法稳定、快速、简便,为细毡毛忍冬的质量控制和开发利用提供了科学依据.     

黄岑同源四倍体株系中黄芩苷含量的比较

黄岑为唇形科植物黄岑（Scutellaria baicalensisGeorgi)的干燥根,研究对离体组织培养条件下诱导获得的黄岑四倍体株系,在进行田间农艺性状的观察比较的同时,用高效液相色谱测定了20个黄岑四倍体株系中黄岑苷的含量。结果表明：在供试的20个黄岑四倍体株系中,有1个株系的黄岑苷含量高于二倍体,大部分与二倍体接近,但多倍体株系的根部产量明显大于二倍,体,因此从大部分株系的有效成分黄岑苷生产量看,大多数四倍体明显优于二倍体,这一结果为今后进一步选育产量高,黄岑苷含量高的优良黄岑品种展示了较好的前景。     

姜四倍体育种的品质评价

将选育获得的5个姜四倍体和二倍体株系试管苗移栽到试验田生长15个月后测定产量性状和主要成分含量,再使用采收的姜块嫩芽建立无菌试管苗,通过根尖染色体鉴定方法观察根尖染色体数目;并将新建立的无菌试管苗再次移栽试验田生长15个月,考察产量性状、主要成分含量和染色体数目的稳定性.结果表明,姜四倍体的产量显著高于二倍体,两次移栽...     

青蒿同源四倍体的诱导及农艺性状的评价

以青蒿组培苗幼嫩顶芽为材料,研究了秋水仙素诱导染色体倍性变异的最佳浓度和最适处理时间,结果表明:用0.2%秋水仙素溶液处理其试管苗幼嫩顶芽24 h的诱导方法最有利于四倍体的产生,诱导率达到36%.显微镜观察变异植株根尖染色体数目为2n=4x=36条,苗床生长2个月后,四倍体植株农艺性状较二倍体有明显的优越性.     

Degraded and undegraded carrageenans and experimental gastric and duodenal ulceration

The prevention of histamine-induced gastric and duodenal ulceration in the guinea-pig has been examined using a series of undegraded and degraded carrageenans. Undegraded carrageenans were active at lower doses than degraded carrageenans. The high viscosity of the undegraded carrageenans in solution prevented their use in larger doses. Degradation of carrageenan without serious loss of sulphate, gives a product which allows the dose to be increased to an extent that its effect more than offsets the slight loss in activity caused by the degradation. No single feature of carrageenan structure can be related to anti-ulcer activity although degradation, and hence reduction of molecular size, generally reduces activity. Sulphate contents over 30% have little apparent effect on activity; κ-carrageenans were not consistently different in anti-ulcer activity from Λ-carrageenans. This contrasts with the antipeptic activity of carrageenans where κ-carrageenans are less active than their Λ-counter-parts. As with antipeptic activity, the degree of anti-ulcer activity is probably determined by a combination of structural features which includes molecular size and polyanionic properties.     

Affective and Anxiety Disorders and Alcohol and Drug Dependence:

Depression and anxiety frequently coexist in patients with substance use disorders. This clinically-oriented article examiens the relationship between these conditions and emphasizes data showing that substances of abuse can cause signs and symptoms of both depression and anxiety. These substance-related syndromes appear to have a different course and prognosis than uncomplicated, independent anxiety and major depressive disorders, and clinicians should consider the role of alcohol and other drugs in all patients presenting with these complaints. The authors will also outline an approach for diagnosing and managing patients with the combination of a substance use and depressive or anxiety disorder.     

Alcohol and Substance Use and Abuse in Women and Children

No abstract available for this article.     

Synthesis and anti-nociceptive and anti-inflammatory effects of gaultherin and its analogs

The synthesis of gaultherin (1) and its analogs was carried out to provide 11 glycosides under phase-transfer catalytic conditions. The activities of all synthesized compounds were evaluated by nitric oxide production inhibitory assay in vitro. Methyl 2-O-(4-O-β-d-galactopyranosyl)-β-d-glucopyranosylbenzoate (5f) showed significantly anti-nociceptive and anti-inflammatory effects by the evaluation in vivo. Structure–activity relationships within these compounds were discussed.