人乳头瘤病毒感染的阴道细胞病理变化

目的 提高人乳头瘤病毒（ＨＰＶ）感染的阴道细胞病理学诊断水平。方法 对１５７例外阴、宫颈活体组织及宫颈／阴道细胞涂片进行组织病理及细胞病理诊断,并对其中的１１２例进行了６Ｂ／１１型ＤＮＡ原位杂交及ＨＰＶ１６、１８型ＤＮＡ ＰＣＲ检测。结果ＨＰＶ ＤＮＡ阳性者８２例。组织病理和宫颈／阴道细胞病理检查为ＨＰＶ感染者,ＨＰＶ ＤＮＡ均为阳性,组织病理检查为ＨＰＶ感染,６２．５０％可经阴道细胞病理检查发     

宫颈癌及癌旁组织中人乳头瘤病毒DNA检测

目的：探讨人乳头瘤病毒（ｈｕｍａｎ ｐａｐｉｌｌｏｍａｖｉｒｕｓ,ＨＰＶ） 与宫颈癌及癌旁组织的关系。方法：应用原位ＰＣＲ技术（ＩＳＰＣＲ）对４４ 例宫颈癌、１８ 例癌旁组织及３０ 例慢性宫颈炎和１５ 例正常宫颈组织中的ＨＰＶ１６ＤＮＡ和ＨＰＶ１８ＤＮＡ进行检测。结果：４４ 例宫颈癌组织中ＨＰＶ１６ＤＮＡ 阳性３５ 例（７９－５％ ）,ＨＰＶ１８ＤＮＡ 阳性５ 例（１１－３ ％） 。其中ＨＰＶ１６ＤＮＡ和ＨＰＶ１８ＤＮＡ均阳性４ 例;ＨＰＶ１８ＤＮＡ 阳性而ＨＰＶ１６ＤＮＡ阴性１ 例;ＨＰＶ１６ＤＮＡ 阳性而ＨＰＶ１８ＤＮＡ 阴性３１ 例。１５ 例癌旁组织伴宫颈上皮内新生物（ｃｅｒｖｉｃａｌｉｎｔｒａｅｐｉｔｈｅｌｉａｌ ｎｅｏｐｌａｓｍ , ＣＩＮ） Ⅱ～Ⅲ级,ＨＰＶ１６ＤＮＡ 阳性１２ 例（８０％ ）,其他３ 例癌旁组织中１ 例ＨＰＶ１６ＤＮＡ阳性;３０ 例慢性宫颈炎有４ 例ＨＰＶ１６ＤＮＡ 阳性（１３－３％ ） ;１５ 例正常宫颈组织ＨＰＶＤＮＡ均阴性。结论：宫颈癌及癌旁伴ＣＩＮⅡⅢ与ＨＰＶ１６、１８ 感染有密切关系,以ＨＰＶ１６ 感染为主     

人乳头瘤病毒感染的多灶性的临床分析

对１７６例外阴尖锐湿疣患者的子宫颈进行检测，以诊断是否同时伴有人乳头状瘤病毒（ＨＰＶ）感染。结果：肉眼观察诊断宫颈ＨＰＶ感染２３例（１３％），阴道镜诊断１４６例（８３％），宫颈活检病理诊断１１２例（６４％），其中合并宫颈上皮内瘤变（ＣＩＮ）１￣２级３２例（１８％）。结果提示：（１）外阴尖锐湿疣患者子宫颈伴有ＨＰＶ感染和／或亚临床感染相当常见。（２）借助阴道镜取宫颈活检是诊断宫颈ＨＰＶ感染的常用方法     

宫颈湿疣的临床病理与HPV亚型分析

目的 探讨宫颈温疣的临床类型及组织病理学特征与人乳头瘤病毒（ＨＰＶ）亚型的关系。方法 对１３０例宫颈湿疣进行病理形态学观察,同时采用ＰＣＲ技术进行ＨＰＶ６／１１、ＨＰＶ１６／１８检测。结果 扁平型宫颈湿疣与ＨＰＶ６／１１感染密切相关（Ｐ〈０．０１）,凹空细胞呈轻度异型性。乳头型宫颈湿疣与ＨＰＶ１６／１８密切相关（Ｐ〈０．０１）,凹空细胞呈重度异型性。结论 宫颈湿疣的临床类型及组织病理学特征与ＨＰＶ     

宫颈癌病理学类型与人乳头瘤病毒感染型别的关系

探讨宫颈癌病理学类型与人乳头瘤病毒感染型别的关系。方法每例宫颈癌标本取样两份,一份进行组织病理学检查,包括ＨＥ、ＰＡＳ和粘液卡红染色;另一份用于提取ＤＮＡ,分别用于ＨＰＶ－１６和ＨＰＶ－１８基因组探讨针进行核酸杂交。结论宫颈鳞癌与ＨＰＶ－１６感染密切相关,宫颈腺癌与ＨＰＶ－１８感染密切相关;而宫颈鳞癌中的不同类型与ＨＰＶ－１６之间的以及宫颈腺癌中的不同类型与ＨＰＶ－１８之间均无明显的相关性。     

HPV核酸探针斑点杂交技术在诊断尖锐湿疣中的应用

目的;评估人类乳头瘤病毒（ＨＰＶ）核酸斑点杂交技术在诊断尖锐湿疣中的价值。方法：采用地高辛标记的ＨＰＶ分型核酸探针斑点杂交技术对５０例外阴、有道尖锐湿疣和２６例保存的尖锐湿疣病理石蜡标本进行ＨＰＶ ＤＮＡ检测。结果：５０例临床是性显８４．０％,２６例病理标本阳性率为８４．６％,差异无显著性（Ｐ〉０．０５）。结论：斑点杂交技术结合组织病理学检查对尖锐湿疣有诊断价值。     

宫颈HPV亚临床感染的病理形态特点及其与HPV亚型的相关性

目的：探讨宫颈人乳头状瘤病毒（ＨＰＶ）亚临床感染的病理变化与ＨＰＶ亚型的关系。方法：观察分析１０２例宫颈ＨＰＶ亚临床感染的病理特点,其中５８例同时取宫颈脱落细胞及分泌物,采用ＰＣＲ方法检测ＨＰＶ６／１１及ＨＰＶ１６／１８,将２组结果进行对比分析。结果：宫颈ＨＰＶ亚临床感染的病理改变不完全等同于临床型尖锐湿疣,除表现Ⅰ型诊断性挖空细胞（４１．２％）外,另可见异型性明显的Ⅱ型非典型性挖空细胞（５８．８％）。宫颈ＨＰＶ感染同时合并ＣＩＮ４２便（４１．２％）,３１例ＨＰＶ感染伴ＣＩＮ２－３病变中有２６例（８３．９％）含Ⅱ型挖空细胞。３６例含Ⅱ型挖空细胞的病变中有３１例（８６．１％）ＨＰＶ１６／１８阳性。结论：Ⅱ型挖空细胞与高危型ＨＰＶ１６／１８及ＣＩＮ２－３密切相关,当出现Ⅱ型挖空细胞时提示ＨＰＶ１６／１８感染的可能性     

153例女阴尖锐湿疣及假性湿疣病理学与HPVDNA原位杂交的对比研究

对１５３例病理学诊断为女阴尖锐湿疣,湿疣样病变及假性湿疣的材料进行地高辛标记的ＨＰＶ６Ｂ／１１ＤＮＡ原位杂交及免疫组化ＨＰＶ抗原检测。结果显示：１１５例尖锐湿疣中１１３例（９８．２６％）ＨＰＶＤＮＡ阳性,１８例湿疣样病变４例（２２．２２％）ＨＰＶＤＮＡ阳性,２０例假性湿疣原位杂交均为阴性。提出特征性的挖空细胞是组织学诊断的重要依据,而地高辛标记的ＨＰＶＤＮＡ原位杂交检测有助于尖锐湿疣与假性湿疣的诊     

GPPCR-RDB法对HPV感染的快速基因诊断

目的：对人乳头瘤病毒（ＨＰＶ）感染进行快速的基因诊断。方法：采用通用引物介导的聚合酶链反应（ＧＰＰＣＲ）扩增皮损组织ＤＮＡ,并同时将扩增产物标记上地高辛配基,再结合扩增产物的非放射性反抽斑点杂交技术,对ＨＰＶ亚临床感染和假性湿疣进行了快速的基因分型诊断及鉴别诊断。结果：１４例亚临床感染中ＨＰＶ６阳性１２例,ＨＰＶ１１阳性４例,ＨＰＶ１６未检测出,所用探针以外型别１例,混合感染５例;１１例假性湿疣中     

人乳头瘤病毒DNA在原发性肺癌组织中的表达

目的：探讨人乳头瘤病毒（ＨＰＶ）ＤＮＡ在原发性肺癌组织中的表达．方法：利用西京医院病理科２７５例肺癌病理标本进行研究,以同期３２例支气客粘膜鳞状化生和３４例粘膜慢性炎症标本作为双非肿瘤对照组．采用随机引物法制备地高辛标记ＨＰＶ６Ｂ／１１,１６,１８,３１型ＤＮＡ探针,应用原位杂交检测肺癌组织中的ＨＰＶ－ＤＮＡ存在情况．结果：ＨＰＶ感染总阳性率为３３．８％（９３／２７５）而各细胞类型阳性率为：肺鳞癌     

应用PCR技术检测尖锐湿疣患者HPV感染

取尖锐湿疣的３０例患者外阴湿疣标枉和宫颈分泌物及同期非尖锐湿疣患者宫艰苦泌物，用聚合酶链反应技术检测其中ＨＰＶ６．１１ＤＮＡ和ＨＰＶ１６．１８ＤＮＡ。结果表明，尖锐湿疣患者宫颈人乳头瘤病毒检出率显著高于非尖锐湿疣患者，以ＨＰＶ６．１１为主，ＨＰＶ１６．１８在二者之间无差别。提示外阴尖锐湿疣主要由ＨＰＶ６．１１感染引起，应用ＰＣＲ技术检测湿疣组织中ＨＰＶ ＤＮＡ可为痢疾诊断提供客观依据。     

HPV Infection and Distribution of HPV Genotypes on Patients with Condyloma Acuminatum

Objective To determine the incidence of infection with HPV and the distribution of HPV genotypes on patients with Condyloma acuminatum. Methods Twenty-three different HPV types were detected by PCR and reverse dot blot （RDB） hybridization over all 6 508 samples of vulva and cervix uteri in patients with condyloma acuminatum. Including 18 types were high-risk （HR）-HPV （16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 83 and MM4）, and 5 types were low-risk （LR）-HPV （6, 11, 42, 43 and 44）. Results Among 6 508 cases, there were 3 288 cases with HPV infection and the incidence rate was 50.52%. The positive HPV patients overlap all 23 genotypes detected. There were 2 038 cases infected with a single HPV type and 1 250 cases infected with multiple HPV types. The constituent ratios in positive cases were 61.98% and 38.02%, respectively. There were 1 453 cases only with LR-HPV types infection, 945 cases only with HR-HPV types and 890 cases both with LR-HPV and HR-HPV infection, and the constituent ratios were 44.19%, 28.74% and 27.07%. There were 4 843 times positive HPV infection, including HR-HPV 2 361 times and LR-HPV 2 482 times. The common HR-HPV genotypes were 16, 52, 58, 56, 18, 66 and 33, and the incidence ratios were 6.31%, 5.06%, 4.04%, 2.60%, 2.41%, 2.40% and 2.28%. And the common LR-HPV genotypes were 6, 11 and 43, and the incidence ratios were 16.98%,11.09% and 6.75%, respectively. Overlap 23 types, the most common geno- types were HPV6 and HPVll, the incidence rates were higher than others （P〈0.05）. Conclusion HPV infection, especially with HR-HPV genotypes infect genital tract, which caused Condyloma acuminatum and cervical lesions, or cervical cancer. The detection of HPV genotypes was very important to prevent, diagnose early and therapy for cervical lesions or cervical cancer.     

宫颈病变检测人乳头瘤病毒感染的意义

目的 探讨人乳头瘤病毒（HPV）感染与宫颈病变的关系.方法 用荧光定量PCR法检测15例正常宫颈,57例宫颈尖锐湿疣,78例宫颈上皮内瘤变（CIN）,43例宫颈尖锐湿疣合并CIN,13例宫颈癌的HPV感染情况.结果 本组宫颈病变中HPV感染率92.72％,单一型HPV感染率89.52％,高危型HPV感染率98.25％;宫颈尖锐湿疣以HPV52感染为主（41.18％）;CIN以HPV16感染为主（41.67％）;尖锐湿疣合并CIN感染HPV58者占25.00％,感染HPV16和HPV52的各占15.00％; 60.00％宫颈癌感染HPV16;多重型HPV感染以HPV亚型双重感染为主.结论 HPV检测对于宫颈病变的筛查及临床分流监测有重要价值.     

佛山地区男性尖锐湿疣患者HPV感染现状及基因型分布

目的 探讨佛山地区男性尖锐湿疣患者中人乳头瘤状病毒（HPV）的感染情况、基因型别及其临床意义。方法 选择500例临床诊断为尖锐湿疣的男性患者,从送检样本中提取病毒基因组DNA,通过聚合酶链式反应对病毒DNA进行扩增后,利用基因芯片对扩增产物进行导流杂交和分型检测。使用Excel对数据进行整理,并用SPSS 16.0统计软件对数据进行统计学分析。结果 500例尖锐湿疣男性患者中有388例检出HPV DNA阳性,其检出率为77.6%(388/500)。感染人群主要集中在21~40岁年龄段,占55.8%（279/500）。检出HPV阳性的患者中,低危型HPV感染291例,检出率为58.2%(291/500);高危型HPV感染仅有16例,检出率为3.2%(16/500);混合型HPV感染81例,检出率为16.2%(81/500)。单一型别感染291例,检出率为58.2%（291/500）;多重感染97例,检出率为19.4%(97/500)。HPV单一感染患者中,感染亚型主要是HPV 6型,HPV多重感染以二重感染和混合型感染为主。结论 佛山地区男性尖锐湿疣患者HPV感染以感染低危型HPV 6型为主,好发年龄段主要集中在21~40岁;单一感染居多,多重感染则以混合型二重感染为主;且HPV感染引起的尖锐湿疣具有一定的地域性差异。     

Histological and cytological evidence of viral infection and human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and normal tissue in the west of Scotland: evaluation of treatment policy

Biopsy samples from 27 patients referred to a colposcopy clinic in Glasgow for cervical abnormalities were assessed for the relations among colposcopic appearances, cytological and histological diagnosis, expression of papillomavirus antigen, and the presence of human papillomavirus (HPV) types 6, 11, 16, and 18 deoxyribonucleic acid (DNA) sequences. Specimens were from colposcopically abnormal areas of the transformation zone and from colposcopically apparently normal areas of the zone in the same patients (paired matched internal control tissue). All 27 women referred for abnormal smears had colposcopic abnormalities. HPV-16 or 18 DNA sequences were detected in 20 of the 27 colposcopically abnormal biopsy samples and 13 of the 27 paired normal samples. Twelve samples of colposcopically normal tissue contained histological evidence of viral infection but only four of these contained HPV DNA sequences. The other nine samples of colposcopically normal tissue which contained HPV DNA sequences were, however, histologically apparently normal. HPV-6 and 11 were not detected. Integration of the HPV-16 genome into the host chromosome was indicated in both cervical intraepithelial neoplasia and control tissues. In two thirds of the HPV DNA positive samples the histological grade was classed as normal, viral atypia, or cervical intraepithelial neoplasia grade 1. Papillomavirus antigen was detected in only six of the abnormal and three of the normal biopsy samples, and HPV DNA was detected in all of these. The detection of HPV DNA correlates well with a combination of histological and cytological evidence of viral infection (20 of 22 cases in this series). A poor correlation between the site on the cervix of histologically confirmed colposcopic abnormality and the presence of HPV DNA sequences implies that a cofactor other than HPV is required for preneoplastic disease to develop. A separate study in two further sets of biopsy samples examined the state of HPV DNA alone. The sets were (a) 43 samples from cervical intraepithelial neoplasia and nine external controls and (b) 155 samples from cervical intraepithelial neoplasia, cervical cancer, vulval intraepithelial neoplasia, and vulval cancer and external controls. HPV-11 was found in only two (4.7%) of the 43 specimens from cervical intraepithelial neoplasia, whereas HPV-16 was found in 90 (58%) of the other 155 specimens. These results also suggest that HPV subtype is subject to geographical location rather than being an indicator of severity of the lesion or of prognosis.     

外阴尖锐湿疣患者宫颈HPV-DNA的检测分型

目的 探讨外阴尖锐湿疣（CA）患者同时伴有宫颈人乳头瘤病毒（HPV）感染的可能性，为宫颈癌的防治提供理论依据。方法 应用基因芯片技术，对58例外阴CA患者宫颈HPV-DNA亚型（18种高危型和5种低危型）进行分型检测；用同期无外阴CA的42例宫颈HPV-DNA作对照。结果 外阴CA患者件宫颈HPV占79．3％，明显高于对照组的19．0％（P〈0．001）；外阴CA患者官颈糜烂较光滑者更多伴有宫颈HPV感染（P〈0．05）。结论 外阴尖锐湿疣患者宫颈HPV感染常见；对外阴尖锐湿疣患者在治疗外在疣体的同时，更应重视宫颈HPV-DNA检测及宫颈糜烂情况，并进行相应的治疗。     

用~(32)P标记的人乳头瘤病毒DNA检测宫颈病变组织DNA中病毒序列

使用Southern blot杂交方法对33例宫颈病变标本进行了检测,发现在26例宫颈癌中,6例存在HPV16DNA同源序列,占23％。HPV18同源序列占7.6％(2/26)。HPVX1同源序列为11.5％(3/26)。HPV16相关序列为27％(7/26)。而在4例慢性宫颈炎中仅有1例含HPV16相关序列,1例尖锐湿疣中含HPV16相关序列。     

用32P标记的人乳头瘤病毒DNA检测宫颈病变组织DNA中病毒序列

使用Southern blot杂交方法对33例宫颈病变标本进行了检测,发现在26例宫颈癌中,6例存在HPV16DNA同源序列,占23%。HPV18同源序列占7.6%(2/26)。HPVX1同源序列为11.5%(3/26)。HPV16相关序列为27%(7/26)。而在4例慢性宫颈炎中仅有1例含HPV16相关序列,1例尖锐湿疣中含HPV16相关序列。     

HPV感染及DAPK甲基化与宫颈病变的相关性研究

目的：研究人乳头瘤病毒(HPV)感染及死亡相关蛋白激酶(DAPK)甲基化与宫颈病变的相关性。方法选择2012年6月至2015年6月在我院接受诊治的310例疑似宫颈病变患者作为研究对象。依照阴道镜的宫颈活检结果进行分组,主要包含宫颈上皮内瘤变Ⅰ~Ⅲ级(CINⅠ~Ⅲ)186例,鳞状细胞癌(SCC)36例,以及未发现恶性细胞及上皮内病变细胞(NILM)88例。检测并对比各组受试者不同宫颈病变对应的HPV感染情况、DAPK甲基化率,分析二者与宫颈病变的相关性。结果310例不同的宫颈病变患者中,共检出212例高危HPV感染阳性者,阳性率为68.39%;伴随宫颈病变程度进展,高危HPV感染阳性率亦表现为增高趋势[NILM：31.82%(28/88);CINⅠ：69.51%(57/82);CINⅡ：85.25%(52/61);CINⅢ：90.70%(39/43);SCC：100.00%(36/36)],组间差异有统计学意义(P<0.05);59例DAPK甲基化阳性者,甲基化率为19.03%;伴随宫颈病变程度进展,宫颈组织DAPK基因甲基化率亦表现为增高趋势[NILM：3.41%(3/88);CINⅠ：9.76%(8/82);CINⅡ：13.11%(8/61);CINⅢ：44.19%(19/43);SCC：58.33%(21/36)],组间差异有统计学意义(P<0.05)。根据Pearson法分析相关性可知,HPV感染与DAPK甲基化及宫颈病变均呈正相关(P<0.05),DAPK甲基化与宫颈病变亦呈正相关(P<0.05)。结论 HPV感染及DAPK甲基化均与宫颈病变具有紧密联系,临床诊断与治疗时均可对其进行持续监测,值得关注。