枇杷叶不同炮制品中熊果酸含量的测定

目的了解不同炮制方法的枇杷叶炮制品质量。方法采用双波长扫描法对枇杷叶几种炮制品的熊果酸含量进行测定。结果蜜炙、姜炙(姜汁炒和姜汤煮)的炮制样品中熊果酸含量均高于生品。结论为今后制定合理的炮制工艺提供科学依据。     

枇杷叶不同炮制品中熊果酸含量的测定

目的了解不同炮制方法的枇杷叶炮制品质量。方法采用双波长扫描法对枇杷叶几种炮制品的熊果酸含量进行测定。结果蜜炙、姜炙(姜汁炒和姜汤煮)的炮制样品中熊果酸含量均高于生品。结论为今后制定合理的炮制工艺提供科学依据。     

高效液相色谱法测定枇杷叶中齐墩果酸和熊果酸的含量

目的 建立枇杷叶中齐墩果酸和熊果酸的含量测定方法,测定不同品种和叶龄的枇杷叶中齐墩果酸和熊果酸的含量.方法 采用高效液相色谱法(HPLC)测定,色谱柱为Hypersil BDS C18柱,流动相为甲醇-0.2%乙酸铵溶液(85:15),流速为0.6ml/min,检测波长为210nm,柱温25℃.结果 齐墩果酸和熊果酸的平均回收率分别为104.3%、97.5%,相对标准偏差(RSD)值分别为1.2%、2.2%(n=6).结论 本法简便、灵敏、快速,可作为枇杷叶药材的质量控制方法之一.     

山楂中熊果酸的含量测定

目的：本实验用95％的乙醇为溶剂,用超声波提取的方法对山楂中的熊果酸进行提取。方法：双波长扫描测定入λ1=535nm,λ2=650nm。结果：熊果酸在0．1～5μg范围内具有良好的线性关系,平均加样r=0．9995。回收率为100．10％,RSD=1．94％（n=3）。结论：该方法安全、准确、经济、可靠。     

HPLC-ELSD法测定枇杷叶中齐墩果酸和熊果酸的含量

目的建立枇杷叶中熊果酸和齐墩果酸含量的HPLC-ELSD测定法。方法采用C18色谱柱(250mm×4.6mm,5μm),以甲醇-水-冰乙酸-三乙胺(90∶10∶0.03∶0.06)为流动相,流速为1.0mL·min-1,柱温为30℃,蒸发光散射检测器飘移管温度为100℃。结果熊果酸在0.244~7.335μg范围内线性关系良好(r=0.9997,n=6),回收率为96.0%;齐墩果酸在0.118~3.525μg范围内线性关系良好(r=0.9998,n=6),回收率为95.2%。结论该方法测定准确,重现性好,可用于枇杷叶的质量控制。     

三安胶囊中熊果酸的含量测定

采用薄层扫描法对样品中车前草的熊果酸进行定量分析。方法简单，准确，可有铲控制该产品的质量。     

十味益脾颗粒中熊果酸含量测定

目的建立十味益脾颗粒含量测定方法。方法采用薄层扫描法测定颗粒剂中熊果酸含量。结果熊果酸点样量在0.66~3.30μg范围内线性关系良好。同板精密度为1.3%异板精密度为2.6%。平均回收率为96.5%(RSD为1.6%)。结论方法简便可靠,可用于十味益脾颗粒的质量控制。     

十味益脾颗粒中熊果酸含量测定

目的建立十味益脾颗粒含量测定方法.方法采用薄层扫描法测定颗粒剂中熊果酸含量.结果熊果酸点样量在0.66～3.30 μg范围内线性关系良好.同板精密度为1.3%异板精密度为2.6%.平均回收率为96.5%(RSD为1.6%).结论方法简便可靠,可用于十味益脾颗粒的质量控制.     

枇杷叶中熊果酸的提取与分离

目的研究提取和分离枇杷叶中熊果酸的方法。方法用95%乙醇超声波提取3次，每次30 min，回收乙醇，浸膏分别用石油醚、1%氢氧化钠溶液和水洗至洗出液无色，再用无水乙醇加热溶解，加活性炭回流脱色15 min，滤过。用热乙醇反复洗涤残渣，放冷，静置，得白色片状结晶，用热甲醇重结晶，高效液相色谱法检测结晶纯度。结果从200 g枇杷叶原药材中得到0.85 g熊果酸结晶，所得熊果酸纯度为94.0%。结论该法可提取得到较纯的熊果酸结晶。     

薄层扫描法测定左归丸中熊果酸含量

目的建立左归丸中熊果酸的含量测定方法。方法双波长薄层扫描法,以环己烷-氯仿-乙酸乙酯-冰醋酸（20：5：8：0．5）为展开剂,测定波长543nm,参比波长700nm。结果平均回收率96．33％（RSD=1.03％）。标准曲线r=0．999,线性范围0．30445—3．0445μg。结论该法准确、重复性好,可作为左归丸的质控方法。     

Paracetamol release from spray-dried products prepared with acrylic resins

Ethanolic solutions of various paracetamol (acetaminophen)/Eudragit E, S, and RS ratios were spray-dried to obtain solid particulates. The products were tableted and the dissolution test of the tablets in distilled water and test solution (pH 1.2 and 7.2) were evaluated. The quantities and types of polymer as well as the dissolution media influenced the drug release. Spray-drying technique provided by the different types of Eudragits was an effective method for prolonging the release rate of paracetamol.     

Quantitative and qualitative HPLC analysis of thermogenic weight loss products

An HPLC qualitative and quantitative method of seven analytes (caffeine, ephedrine, forskolin, icariin, pseudoephedrine, synephrine, and yohimbine) in thermogenic weight loss preparations available on the market is described in this paper. After 45 min the seven analytes were separated and detected in the acetonitrile: water (80:20) extract. The method uses a Waters XTerra RP18 (5 microm particle size) column as the stationary phase, a gradient mobile phase of water (5.0 mM SDS) and acetonitrile, and a UV detection of 210 nm. The correlation coefficients for the calibration curves and the recovery rates ranged from 0.994 to 0.999 and from 97.45% to 101.05%, respectively. The qualitative and quantitative results are discussed.     

不同工艺制备延胡索提取物的FTIR谱图特征分析

目的考察延胡索不同提取物粉末的红外光谱学特征,建立延胡索提取物的质量检测方法,为中药提取物的实时质量监控提供参考。方法以延胡索乙素为指标,采用HPLC测定其含量;利用傅里叶变换红外光谱技术(FTIR)对延胡索药材及其不同提取物进行研究。结果延胡索药材及其提取物有着各自稳定的红外光谱指纹特征,图谱中位于1 610,1 520和1 457cm-1处的振动峰是判断延胡索乙素在不同提取物样品中含量高低的主要依据,而其测定结果与HPLC的结果基本吻合。结论该方法简便、快速,能够准确地提供中药提取物中主要化学成分的相关信息,为制定下一步的提取方案提供方向性参考。     

Triboelectrification of spray-dried lactose prepared from different feedstock concentrations

Powder systems may acquire electrostatic charge during various pharmaceutical processing operations and may give rise to difficulties in handling and powder flow, mainly due to adhesion/cohesion effects. We have investigated the electrostatic charging of spray-dried lactose prepared from different feedstock concentrations using a laboratory spray-dryer. Triboelectrification of the spray-dried lactose samples was effected through contact with the stainless steel surface of either a mixing vessel or a cyclone separator. Results from both techniques showed differences in charge accumulation and particle-steel adhesion between the spray-dried lactose samples. As the feedstock concentration used to produce the spray-dried lactose was increased in the range 10-50% w/v, the mean charge on the lactose decreased from -20.8 to -1.3 nC g(-1) and -54.9 to -4.1 nC g(-1) for the mixing vessel and cyclone separator, respectively, with a corresponding decrease in adhesion. In addition, as the feedstock concentration was increased from 10 to 50% w/v, decreases were obtained in surface area values (1.06 to 0.56 m2 g(-1)), pore diameter (198.7 to 83.5 microm) and pore volume (1.09 to 0.75 cm3 g(-1)), and together with differences in crystal form correlated with the charge and adhesion results. The results suggested that the feedstock concentration could have a considerable influence on the charging and adhesional properties of spray-dried lactose. This may have relevance during pharmaceutical processing and manufacturing operations.     

Quantitative analysis of degradation products in pilocarpine hydrochloride ophthalmic formulations.

The presence of isopilocarpine, an epimer of pilocarpine, and of pilocarpinic acid, a hydrolytic degradation product of pilocarpine, was established and all three substances were assayed in various commercial ophthalmic formulations of pilocarpine hydrochloride by 13C-Fourier transform spectroscopy. Assay was based upon integrated intensities of selected resonances from any formulation calibrated against the intensity of tetramethylammonium bromide, used as a common external reference. The normalized intensities were then related to those of a reference solution of pilocarpine hydrochloride, thereby eliminating any factor arising from variability of 13C-relaxation times. The 13C-resonance for the N-methyl group, being common to all products, provides a convenient basis for the assay of the total alkaloid content whereas the C-8 resonances are best suited for assaying residual pilocarpine and its degradation products. This procedure, estimated as accurate to +/- 5%, constitutes the first comprehensive analytical method to differentiate between pilocarpine and its degradation products.     

高效液相色谱法测定不同产地白花蛇舌草中齐墩果酸和熊果酸的含量

目的分析不同产地白花蛇舌草中齐墩果酸和熊果酸含量,为药材及其制剂的质量控制提供参考。方法采用高效液相色谱法(HPLC)测定。色谱柱AgilentC18柱,流动相为甲醇-水-冰醋酸-三乙胺(91.5∶8.5∶0.04∶0.1),检测波长210nm,流速0.80ml/min,柱温20℃。结果齐墩果酸和熊果酸分别在20.4～326.4μg/ml(r=0.9991)和39.2～627.2μg/ml(r=0.9994)范围内线性关系良好;齐墩果酸和熊果酸的平均回收率在98.0%～99.0%,相对标准差(RSD)分别为0.87%和1.22%。结论本方法准确、简便、重现性好,可同时测定白花蛇舌草中齐墩果酸和熊果酸的含量,能够应用于药材及其制剂的质量控制。     

高效液相色谱法测定栀子及其炮制品中熊果酸的含量

目的 建立栀子及其炮制品中熊果酸的测定法,考察栀子炮制前后熊果酸的含量变化。方法 采用高效液相色谱法,色谱柱为Hypersil C_(18)5μm(4.6mm×250mm),流动相为0.02mol/L NaH_2PO_4(pH2.6):甲醇(16:84),检测波长220mm。结果 在23～135μg/ml范围内,呈良好的线性关系,平均加样回收率和RSD分别为97.81％、1.51％。栀子炮制后熊果酸含量下降。结论 该方法可用于栀子及炮制品的质量控制,炮制可导致栀子中熊果酸的含量下降。     

高效液相色谱法测定栀子及其炮制品中熊果酸的含量

目的建立栀子及其炮制品中熊果酸的测定法,考察栀子炮制前后熊果酸的含量变化.方法采用高效液相色谱法,色谱柱为Hypersil C18 5μm(4.6 mm×250 mm),流动相为0.02 mol/L NaH2PO4(pH2.6):甲醇(16:84),检测波长220 mm.结果在23～135 μg/ml范围内,呈良好的线性关系,平均加样回收率和RSD分别为97.81%、1.51%.栀子炮制后熊果酸含量下降.结论该方法可用于栀子及炮制品的质量控制,炮制可导致栀子中熊果酸的含量下降.