赛庚啶对大鼠血清性激素含量的影响

目的　研究赛庚啶 (CYP)对大鼠血清性激素含量的影响。方法　用放射免疫分析法 (RIA)观察赛庚啶对大鼠垂体 性腺轴内分泌功能的影响。结果　赛庚啶 (2 .3mg·kg-1·d-1,灌胃 ,14d)可明显升高雌性大鼠血清LH的水平 (P <0 .0 5 ) ,降低其卵泡刺激素 (FSH)、孕酮 (P)的含量 (P <0 .0 1) ;对雄性大鼠血清黄体生成素(LH)、FSH、睾酮 (T)的含量均无显著影响。赛庚啶 (4.6mg·kg-1·d-1,灌胃 ,14d)可明显升高雄性大鼠血清LH ,T的含量 (P <0 .0 5 ) ,而对FSH无显著影响 ;同时可显著升高雌性大鼠血清LH的含量 (P <0 .0 5 ) ,降低其FSH ,P ,E2的含量 (P <0 .0 1)。结论　赛庚啶可促进雌雄大鼠LH及雄性大鼠T分泌 ,抑制雌性大鼠FSH ,E2 ,P的分泌 ,对雄性大鼠FSH分泌无显著影响 ,且存在性别差异。     

二甲双胍对大鼠血清性激素含量的影响

目的　观察二甲双胍对大鼠血清性激素含量的影响。方法　用放射免疫分析法 (RIA)观察二甲双胍对大鼠血清性激素含量的影响。结果　二甲双胍 (135mg·kg-1·d-1,灌胃 ,14d)可明显降低雌性大鼠血清黄体生成素 (LH)及雄性大鼠睾酮 (T)的水平 ,对雌性大鼠卵泡刺激素 (FSH)、雌二醇 (E2 )、孕酮 (P)的含量和雄性大鼠血清LH、FSH的含量均无显著影响。二甲双胍 (2 70mg·kg-1·d-1,灌胃 ,14d)可降低雄性大鼠血清T的含量 ,而对LH ,FSH无显著影响 ;可明显降低雌性大鼠血清LH的含量 ,升高其P ,E2 的含量。结论　二甲双胍可降低雌性大鼠LH及雄性大鼠T水平 ,升高雌性大鼠E2 ,P的含量 ,对大鼠FSH分泌无显著影响     

甲状腺素对雌性大鼠血清性激素含量的影响

目的 观察甲状腺素(T4)对雌性大鼠血清性激素含量的影响.方法 采用放射免疫(RIA)分析法系统观察T4对大鼠血清性激素含量影响.结果 T4100μg/(kg·d),灌胃,连续用药21d,可显著降低大鼠血清黄体生成素(LH)的水平(P＜0.01),而对大鼠卵泡刺激素(FSH)、雌二醇(E2)、孕酮(P)的含量无显著影响.T4 600μg/(kg·d)T4可明显降低雌性大鼠LH,E2,P的水平(P＜0.01),对大鼠FSH的含量无显著性影响(P＞0.05).结论 甲状腺素大剂量、长期应用可降低雌性大鼠血清性激素的水平.     

甲状腺素对胰岛β细胞A20 mRNA表达作用的研究

目的 研究甲状腺素对胰岛β细胞A20 mRNA表达作用的影响,观察大鼠胰岛β细胞形态学变化.方法 采用氧化酶法及放射免疫法测定大鼠血清葡萄糖和血清胰岛素的含量.大鼠胰岛β细胞总DNA琼脂糖凝胶电泳,检测凋亡条带的形成.RT-PCR检测胰岛细胞抗凋亡蛋白A20(TNF-induced protein 3)mRNA的表达.结果 给予T4(600μg·kg-1·d-1)灌胃14d后,可使大鼠血清胰岛素水平明显降低(P＜0.05),血糖水平则明显升高(P＜0.01),并且大鼠胰岛β细胞总DNA琼脂糖凝胶电泳呈现典型凋亡梯状条带.而给予T4灌胃7d时,不发生上述改变.A20 mRNA在给予T4(600μg·kg-1·d-1)灌胃7d和14d组有表达,而且后者明显低于前者(P＜0.01),但在对照组和给予T4灌胃21d组无表达.结论 T4引起胰岛细胞的凋亡可能与抗凋亡蛋白A20在胰岛β细胞的非持续性表达有关.     

巢白金红杞胶囊对衰老大鼠卵巢的功能提高作用

目的：观察天然药物对衰老卵巢内分泌功能的改善作用。方法：以化学发光法检测15月龄雌性SD大鼠用药前、后血清雌激素（E2）、孕激素（P）、促卵泡生长素（FSH）及促黄体生长素（LH）水平改变;观察比较动物用药前、后的垂体、卵巢、子宫脏器系数变化及组织病理学改变。结果：用药组大鼠血清E2、P水平的提高和血清FSH、LH水平的降低均具有统计学意义;阴道脱落细胞呈现中度雌激素影响;子宫系数明显提高;卵巢内发育卵泡和黄体数量增加,颗粒细胞和卵泡膜细胞明显增殖且排列有序。结论：天然药物巢白金红杞胶囊能改善衰老大鼠卵巢的内分泌功能。     

单纯用钙泊三醇后再用钙泊三醇/倍他米松二丙酸盐与Tacalcitol治疗寻常银屑病疗效比较:随机双盲试验

背景:已经有文献报道每天应用1次复方制剂钙泊三醇50μg·g-1和倍他米松二丙酸盐0.5m g·g-1(D aivobet,Dovobet)治疗寻常银屑病有效。目的:比较应用钙泊三醇4周后再用钙泊三醇或倍他米松二丙酸盐4周与应用Tacalcitol8周治疗稳定期寻常银屑病的疗效、安全性。方法:501例患者接受随机双盲试验。应用钙泊三醇50μg·g-1,1次·d-1,连续4周后再用复方制剂4周。或者应用Tacalcitol4μg·g-1,1次·d-1,连续8周。结果:就PA SI降低百分率而言,应用钙泊三醇及其复方制剂治疗组的疗效显著高于Tacalcitol治疗组(4周时分别为65.0%和33.3%,8周时分别…     

慢性焦虑刺激对成年雌性大鼠性激素水平的影响

目的 探讨慢性焦虑刺激对成年雌性大鼠性激素水平的影响.方法 将雌性成年大鼠随机分成空白组、模型组,采用不确定性空瓶刺激法建立焦虑应激大鼠模型.以高架十字迷宫实验评价其行为学;采用化学发光法检测大鼠性激素六项:促黄体生成素(LH),促卵泡激素(FSH),泌乳素(PRL),睾酮(T),孕酮(P),雌二醇(E2)水平;光镜观察大鼠子宫、卵巢的形态学变化.结果 与空白组相比,模型组大鼠血清T浓度显著升高(P＜0.01),LH浓度显著减少(P＜0.01),FSH、PRL、P、E2水平差异无统计学意义;模型组卵巢组织闭锁卵泡多,黄体和成熟卵泡少,子宫内膜较薄,间质纤维增生.结论 在焦虑状态下,成年雌性大鼠的血清T浓度升高,LH浓度降低,子宫、卵巢出现病理改变,提示焦虑是影响雌性大鼠生殖机能的重要因素.     

老年前期雌性大鼠下丘脑-垂体-卵巢轴的功能变化

研究雌性老年前期大鼠性腺轴功能的变化．方法：应用阴道细胞形态学检查法和放射免疫测定法观察18月龄雌性SD大鼠动情周期和组织中性激素含量的变化．结果：18月龄雌性大鼠血清E2水平降低，LH和FSH含量升高；下丘脑和血浆GnRH水平上升，垂体GnRH含量减少．结论：18月龄雌性老年前期SD大鼠动情周期停止，下匠脑－垂体－卵巢轴功能明显衰退．     

TNF-α对雌性大鼠生殖轴调控作用的研究

目的　通过动物实验探讨肿瘤坏死因子 (TNF α)在生殖轴调控中的作用及作用部位。方法　情前期成年雌性S D大鼠 4 4只 ,取下丘脑、腺垂体及卵巢分别进行原代细胞培养 ,贴壁后 ,于腺垂体及卵巢培养液中分别加入TNF α (10 0ng/ml) ,孵育 2 4h后 ,进行顺序灌流共 5次 ,将培养液冷冻干燥后按放免试剂盒说明测FSH、LH、E2 及P ,并收集各组培养卵巢颗粒细胞 ,用流式细胞仪检测颗粒细胞凋亡率 ,观察TNF α对生殖轴调控作用及卵巢颗粒细胞增殖的作用的影响。结果　 (1)TNF α (10 0ng/ml)明显抑制原代培养的雌性动情前期大鼠卵巢组织E2 及P的分泌量。 (2 )TNF α (10 0ng/ml)明显抑制原代培养的雌性动情前期大鼠腺垂体细胞LH、FSH的分泌量。 (3)FCM检测结果 :实验组卵巢颗粒细胞凋亡率为 (37 84± 3 84 ) %与对照组 (2 6 3±0 37) %相比有显著性差异 (P <0 0 1) ,说明TNF α能够抑制颗粒细胞增殖 ,并促进其凋亡。结论　TNF α在腺垂体及卵巢水平上同时发挥作用 ,抑制FSH、LH、E2 、P的合成 ,同时抑制颗粒细胞的增殖 ,促进其凋亡。     

宁心红杞胶囊对雌性老龄大鼠垂体-卵巢轴功能的影响

目的宁心红杞胶囊对老龄大鼠雌激素水平及垂体、卵巢、肾上腺的影响。方法14月龄SD雌性大鼠随机分为模型组、阳性药组、宁红低剂量组、宁红中剂量组、宁红高剂量组,4月龄SD雌性大鼠为青年组。给药2个月后,取血、离心,放免（RIA）法测定血清中雌二醇（E2）、孕酮（P）、卵泡刺激素（FSH）、黄体生成素（LH）的水平;垂体、卵巢、肾上腺脏器系数比较。结果宁心红杞胶囊使更年期大鼠血清中原下降的E2水平上升,上升的PSH和LH水平下降,增加垂体、卵巢、肾上腺的质量。结论宁心红杞胶囊能缓解垂体、卵巢、肾上腺的萎缩,调节垂体-卵巢轴功能。      

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.