共查询到10条相似文献,搜索用时 15 毫秒
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Comparison of the digene hybrid capture 2 and Roche cobas 4800 HPV tests for detection of CIN2+ in a referral population in Japan
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Toshiyuki Sasagawa Toshiyuki Maehama Yasuhiro Osaka Jinichi Sakamoto Takeo Shibata Satoko Fujita Masahiro Takakura Hiroaki Takagi 《Journal of medical virology》2018,90(5):972-980
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Sensitivity of APTIMA HPV E6/E7 mRNA test in comparison with hybrid capture 2 HPV DNA test for detection of high risk oncogenic human papillomavirus in 396 biopsy confirmed cervical cancers
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Partha Basu Dipanwita Banerjee Srabani Mittal Sankhadeep Dutta Ishita Ghosh Nilarun Chowdhury Priya Abraham Puneet Chandna Sam Ratnam 《Journal of medical virology》2016,88(7):1271-1278
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Jack Cuzick Laurence Ambroisine Louise Cadman Janet Austin Linda Ho George Terry Stuart Liddle Roberto Dina Julie McCarthy Hilary Buckley Christine Bergeron W.P. Soutter Deirdre Lyons Anne Szarewski 《Journal of medical virology》2010,82(7):1186-1191
HPV DNA testing is known to be much more sensitive than cytology, but less specific. A range of HPV and related tests in 858 women referred for colposcopy because of an abnormal smear were evaluated to compare the performances of these tests. This article compared the Abbott test to other tests which had been previously evaluated. This test was a real true test for 14 high‐risk HPV types. The Abbott test was found to be highly sensitive for cervical intraepithelial neoplasia grade 3 or worse (CIN3+) (98.9%) with a specificity of 31.5%. These numbers were comparable with the Qiagen HC2 test, the Roche Linear Array and Amplicor tests, and the Gen‐Probe APTIMA test. Differences between these tests appeared to be related mostly to the choice of cutoff level. An added feature of the Abbott test was the provision of type specific results for HPV 16 and 18. J. Med. Virol. 82: 1186–1191, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
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Melanie A. Ruffner Seon Hee Kim Nicole R. Bianco Loise M. Francisco Arlene H. Sharpe Paul D. Robbins 《European journal of immunology》2009,39(11):3084-3090
Costimulatory molecules, such as B7‐1/2 and PD‐L1/2 play an important role in the function of APC. The regulation of the surface levels of costimulatory molecules is one mechanism by which APC maintain the balance between tolerance and immunity. We examined the contributions of B7‐1/2 and PD‐L1/2 to the function of IL‐10‐treated, immunosuppressive DC as well as therapeutic exosomes derived from these DC. IL‐10 treatment of DC significantly downregulated surface expression of MHC II, B7‐1, B7‐2, and decreased levels of MHC I and PD‐L2. IL‐10 treatment of DC resulted in a modified costimulatory profile of DC‐secreted exosomes with a reduction in B7‐1, PD‐L1 and PD‐L2. We further demonstrate that absence of B7‐1 or B7‐2 on donor DC results in a loss of ability of IL‐10‐treated DC and their exosomes to suppress the delayed‐type hypersensitivity response, whereas IL‐10‐treated DC deficient in PD‐L1/2 as well as their secreted exosomes retained the ability to suppress delayed‐type hypersensitivity responses. We conclude that B7‐1 and B7‐2, but not PD‐L1 and PD‐L2, on IL‐10‐treated DC and DC‐derived exosomes play a critical role in immunosuppressive functions of both DC and exosomes. 相似文献
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BDCA3 expression is associated with high IFN‐λ production by CD34+‐derived dendritic cells generated in the presence of GM‐CSF,IL‐4, and/or TGF‐β
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Evelyn van der Aa Lianne van de Laar Harry L.A. Janssen Nadine van Montfoort Andrea M. Woltman 《European journal of immunology》2015,45(5):1471-1481
High BDCA3 expression is associated with a specific human IFN‐λ‐producing dendritic cell (DC) subset. However, BDCA3 has also been detected on other DC subsets. Thus far, development and function of BDCA3 expression on DCs remains poorly understood. Human Langerhans cells (LCs) and interstitial DCs (intDCs) can be generated in vitro by differentiation of CD34+ hematopoietic progenitors via distinct precursor DCs (preDCs), CD1a+ preDCs, and CD14+ preDCs, respectively. Here, we identified BDCA3 expression in this well‐known GM‐CSF/TNF‐α‐driven culture system and described the effect of IL‐4 and/or TGF‐β on induction of BDCA3 expression. In control or TGF‐β cultures, BDCA3 was only detected on CD14+ preDC‐derived intDCs. IL‐4 induced BDCA3 expression in both CD14+‐derived and CD1a+‐derived cultures. TGF‐β and IL‐4 together further increased CD14+‐derived and CD1a+‐derived BDCA3+ DC frequencies, which partly expressed CLEC9A, but were not identical to the BDCA3highCLEC9A+ DC subset in vivo. Importantly, BDCA3+ cells, but not BDCA3? cells, in this system produced high IFN‐λ levels upon polyinosinic:polycytidylic acid (polyI:C) stimulation. This culture system, in which BDCA3 expression is preferentially associated with the intDC lineage and IFN‐λ‐producing capacity, will greatly contribute to further research on the function and regulation of BDCA3 expression and IFN‐λ production by DCs. 相似文献
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Doris Mayr Sibylle Heim Kerstin Weyrauch Evelyn Zeindl‐Eberhart Anne Kunz Jutta Engel Thomas Kirchner 《Histopathology》2009,55(6):716-723
Aims: Her‐2/neu testing is used as a marker for Herceptin® therapy. The aim was to investigate new dual‐colour chromogenic in situ hybridization (CISH), in a large number of breast carcinomas (n = 205) with DNA‐specific dual‐colour probes (ZytoVision, Bremerhaven, Germany) and to compare the results with immunohistochemistry (n = 205) and fluorescence in situ hybridization (FISH) (n = 129). Methods and results: Paraffin‐embedded tissue of 205 patients was used. After immunohistochemistry with a focus on immunohistochemically uncertain cases, Her‐2/neu amplification using dual‐colour CISH (ZytoVision®) was analysed. Validation by FISH was performed. The results were: immunohistochemistry, 27.8% with strong expression, 53.7% with uncertain overexpression and 18.5% with no expression; FISH, 25.6% amplified and 74.4% negative; CISH, 35.6% amplified, 62.9% negative and 1.5% not evaluable. Comparison of immunohistochemistry with CISH: CISH negative in 100% with immunohistochemistry 0/1+, amplified in 82.5% with immunohistochemistry 3+; 5.9% contradictory results: 4.4% immunohistochemistry 3+ and negative by CISH, 1.5% negative in immunohistochemistry but amplified by CISH; FISH (129 cases), 8.5% contradictory results to immunohistochemistry, 6.2% immunohistochemistry 3+ and negative by FISH, 2.3% negative by immunohistochemistry and amplified by FISH; comparison of CISH and FISH, 94.6% same results, 3.9% different ones, 1.6% CISH not analysable. Conclusions: CISH, using dual‐colour probes (ZytoVision®) is as good as FISH for Her‐2/neu analysis. The few discrepant results are likely to be caused by polysomy or tumour heterogeneity. 相似文献
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PGC‐1α4 gene expression is suppressed by the IL‐6—MEK—ERK 1/2 MAPK signalling axis and altered by resistance exercise,obesity and muscle injury
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J. L. Brown M. E. Rosa‐Caldwell D. E. Lee L. A. Brown R. A. Perry K. L. Shimkus T. A. Blackwell J. D. Fluckey J. A. Carson S. Dridi T. A. Washington N. P. Greene 《Acta physiologica (Oxford, England)》2017,220(2):275-288