影响飞行员睡眠质量的心理社会因素分析

①目的探讨影响飞行员睡眠质量的心理社会因素。②方法采用临床症状自评量表、卡特尔１６种个性因素问卷、紧张性生活事件量表、Ｚｉｍｅｔ领悟社会支持量表进行集体测评。③结果睡眠状况与个性中的聪慧性、稳定性、兴奋性、有恒性、敢为性、自律性呈负相关（ｒ＝－０．２９～－０．１３，Ｐ＜０．０５或０．０１）；与忧虑性、怀疑性、紧张性呈正相关（ｒ＝０．１４～０．３７，Ｐ＜０．０５或０．０１）；与紧张性生活事件中的婚姻恋爱、健康、家庭、环境因素呈正相关（ｒ＝０．１９～０．３２，Ｐ＜０．０１）；与反映心理健康水平的ＳＣＬ－９０量表中的９个因子以及家庭支持和朋友支持也具有相关性。同时也证实飞行员睡眠状况与飞行时间呈正相关（ｒ＝０．１５，Ｐ＜０．０５）。④结论飞行员睡眠质量与心理社会因素有相关性。     

青少年自我报告的行为,情绪问题15个月随访研究

探讨青少年自我报告的行为、情绪问题的稳定性和变化趋势。方法应用Ａｃｈｅｎｂａｃｈ青少年行为自评量表（ＹＳＲ）对２６６名１２～１７岁的青少年１５个月前后进行了两次评定。结果青少年自我报告的行为、情绪问题总分１５个月内有高度稳定性（ｒ＝０．６０１２），尤以攻击行为和抑郁情绪两个因子为著。１５个月后行为、情绪问题分有升高的趋势。５０％符合行为问题标准的青少年１５个月后仍偏离正常，４．６％的正常青少年达到了行为问题标准。结论青少年行为、情绪问题具有较高的稳定性，提示对这些问题进行早期干预是必要的。     

营养性贫血病儿血清微量元素的变化

①目的　探讨儿童营养性贫血与血清微量元素铁、钴、锌、铅水平的关系。②方法　测定８０ 例营养性贫血病儿血清铁、钴、锌、铅、血红蛋白含量及红细胞计数，并与３３ 例健康儿童进行了比较。③结果　贫血组儿童血清铁、锌水平较对照组明显降低，铅明显增高，差异均有极显著性（ｔ＝ ３．０１４～８．３５３， Ｐ 均＜ ０．０１）； 钴含量差异无显著性（ｔ＝ １．９３３， Ｐ＞ ０．０５）。血清铁含量与轻、中度贫血呈正相关（ｒ＝ ０．５０７，０．９１２， Ｐ＜ ０．０１）；钴、锌含量与中度贫血呈正相关（ｒ＝ ０．８９１，０．８５６， Ｐ＜ ０．０１），与轻度贫血无相关性（ｒ＝ ０．０５２，０．１１６， Ｐ＞ ０．０５）；铅与中度贫血呈负相关（ｒ＝ － ０．６２５， Ｐ＜ ０．０１），与轻度贫血无相关性（ｒ＝ ０．０６０， Ｐ＞ ０．０５）。④结论　治疗营养性贫血病儿应注意相关微量元素水平的合理调整。     

非胰岛素依赖型糖尿病患者运动前后血脂和白蛋白变化对胰岛素受体的影响

为广解急性运动前后血脂和白蛋白水平对胰岛素受体结合的影响．对３８例非肥胖非胰岛素依赖型糖尿病（ＮＩＤＤＭ）患者和３７名正常人进行了３０分钟中等强度运动前后红细胞膜胰岛素受体的测定，并对受体参数与血脂、白蛋白之间进行了相关分析。结果显示急性运动前ＮＩＤＤＭ组高亲和力亲和常数（Ｋ＿１）与总胆固醇和白蛋白水平呈显著负相关（ｒ＝－０．４７３，Ｐ＝０．０１；ｒ＝－０．４８３，Ｐ＝０．０１）。低亲和力亲和常数（Ｋ＿２）与甘油三酯呈显著正相关（ｒ＝０．５０３，Ｐ＝０．０１）．与高密度脂蛋白胆固醇呈显著负相关（ｒ＝－０．４８０；Ｐ＝０．０１），急性运动后血脂和白蛋白浓度的变化与受体参数之间未发现有意义的相关关系。提示ＮＩＤＤＭ病人基础血脂和白蛋白水平是影响胰岛素受体亲和力的重要因素。     

三维超声心动图评价左室容量和心功能的探讨

目的评价三维超声心动图测量左室容量和射血分数的准确性。方法应用ＥｃｈｏＰＡＣ计算机三维重建系统，通过３个互成６０°的左室心尖长轴切面对２０例健康体检者（正常组）和１６例冠心病人（冠心病组）进行左室心内膜三维重建，计算其舒张末期容量（ＬＶＶｄ）和射血分数（ＥＦ），并与Ｍ型法、二维法（双平面法）及核素心血池造影对照，分析不同方法ＬＶＶｄ、ＥＦ测值间的相关性。结果（１）双平面法、三维法同核素心血池造影法的ＥＦ测值间的相关性好（ｒ分别为０．８０和０．８７），（２）在正常组，Ｍ型超声的ＬＶＶｄ、ＥＦ测值同双平面法、三维法结果呈中度相关（ｒ值０．５８～０．６６），双平面法与三维法间高度相关（ｒ＝０．９０和０．８７）；（３）在冠心病组，上述相应的相关系数降低，但双平面法同三维法间依然高度相关（ｒ＝０．８７和０．８４）。结论（１）对于冠心病心脏（心腔形态异常）的容量指标估测，三维超声心动图方法准确性高，优于其他超声方法；（２）本文的三维重建方法简单易行，在容量指标估计方面不亚于其他多平面重建法。     

成都地区437例健康男性中老年人血清载脂蛋白CⅡ、CⅢ及E水平分析

为探讨我国健康男性中老年人血清载脂蛋白（ａｐｏ）ＣⅡ、ＣⅢ及Ｅ的正常参考值，采用单向免疫扩散测定法对成都地区４３７例４０～７０岁健康男性空腹血清ａｐｏＣⅡ、ＣⅢ及Ｅ的含量进行了分析。结果显示：成都地区健康男性中老年人空腹血清ａｐｏＣⅡ、ＣⅢ及Ｅ的含量分别为４１．６±１３．１、１１２．８±３１．０和３８．７±８．２ｍｇ／Ｌ，而且不同年龄组间无显著性差异（Ｐ＞０．０５）。血清ａｐｏＣⅡ、ＣⅢ及Ｅ的水平与ＴＧ、ＴＣ及ＬＤＬ－Ｃ水平呈正相关（ｒ＝０．１２９５～０．５５５４，Ｐ＜０．０１），血清ａｐｏＣⅡ及ＣⅢ水平与体重指数呈正相关（ｒ＝０．１２４８～０．２０７９，Ｐ＜０．０１），ａｐｏＥ水平与年龄呈正相关（ｒ＝０．１４４８，Ｐ＜０．０１）。     

化学发光法测定二氢青蒿素

二氢青蒿素与鲁米诺在碱性条件下不需过氧化氢就能直接发生化学发光反应，氯化血红素对这一新的体系同样具有强烈的催化作用。运用这一新的发光体系建立了灵敏度高，特异性强，操作方便的测定抗疟新药二氢青蒿素的化学发光新方法，线性范围为０～３０００ｎｇ／ｍｌ，线性方程为Ｙ＝１．１４６Ｘ＋２．９３（０～１００ｎｇ／ｍｌ），ｒ＝０．９９４，Ｙ＝２．１７Ｘ－１３９（１００～３０００ｎｇ／ｍｌ），ｒ＝０．９９９，，检出     

精神分裂症患者血清LPO、GPX和VE测定

对５６例精神分裂症患者和４２例正常对照组的血清过氧化脂质（ＬＰＯ）、谷胱甘肽过氧化物酶（ＧＰＸ）和维生素Ｅ（ＶＥ）水平进行了测定，结果表明：ＬＰＯ含量明显高于对照组（Ｐ＜０．０５），ＧＰＸ活性和ＶＥ含量明显低于对照组（Ｐ＜０．０１）；ＬＰＯ与病程呈正相关（ｒ＝０．３６１，Ｐ＜０．０１），ＧＰＸ和ＶＥ与病程呈负相关（ｒ＝一０．３８３，ｒ＝０．４０３，Ｐ＜０．０１）：ＬＰＯ与ＨＰＸ和ＶＥ呈负相关（ｒ＝一０．３７８，ｒ＝０．４１１，Ｐ＜０．０１）．结合文献，认为自由基代谢异常在本质的发病机制中具有一定作用。     

反相高压液相色谱法测定植酸研究

本文建立了反相高压液相色谱测定植酸的方法。采用ＺｏｒｂｏｘＣ８色谱柱和０．０２５ｍｏｌ／Ｌ磷酸二氢钾为流动相，流速为０．５ｍｌ／ｍｉｎ，用５％三氯乙酸抽提样品，抽提液过滤后直接注入色谱柱测定，用紫外检测器检测植酸，检测波长为２５４ｎｍ，在１０～８０μｇ范围内，线性回归方程为Ｙ＝０．０３１７＋０．００９６２８Ｘ（ｒ＝０．９９９５）。本方法应用于食品中植酸测定，与分光光度法所测结果进行比较，差异无显著性（Ｐ＞０．０５）。     

培福新注射液中培氟沙星和维生素C钠的高效液相色谱分析

建立了高效液相色谱法测定培福新注射液中培氟沙星和维生素Ｃ钠的含量，采用Ｃ１８柱，１０μｍ（大连化物所Ｓｐｈｅｒｉｓｏｒｂ），以乙腈－柠檬酸液（０．０５ｍｏｌ／Ｌ）－醋酸铵液（０．５ｍｏｌ／Ｌ）－１％磷酸水溶液（２２∶７５∶１∶２，内含０．１％三乙胺）为流动相，在紫外检测器２５４ｎｍ波长处进行检测。培氟沙星线性范围６０～２４０μｇ／ｍｌ，ｒ＝０．９９９７（ｎ＝５），维生素Ｃ钠线性范围１～９μｇ／ｍｌ，ｒ＝０．９９９９（ｎ＝５）。方法简便，结果满意。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.