热疗联合丝裂霉素对大肠癌细胞的影响

目的 了解热疗联合丝裂霉素对大肠癌细胞的影响。方法 本用MTT法体外研究热疗联合丝裂霉素(MMC)对人大肠癌细胞株LOVO的细胞毒作用。结果 单纯热，43℃30分钟具有较强的细胞毒作用。单纯化疗，在MMC浓度为0．01mg/ml时，具有较强的细胞毒作用。热疔联合化疗，细胞毒作用明显增强，大于单纯热疗和单纯化疗之和，二具有协同作用。43℃30分钟以上，细胞毒作用很强。单纯热疗和单纯化疗在低渗条件下，细胞毒作用大于等渗的细胞毒作用(P<0.05)。结论 经尽可能短的时间，使温度达43℃，持续时间不少于30分钟，MMC浓度不低于0．01mg/ml时，热疗联合丝裂霉素对人大肠癌细胞有很强的细胞毒作用。     

大肠癌热疗联合放化疗临床研究进展

大肠癌是我国常见的恶性肿瘤.随着经济的发展、生活水平的提高和生活方式的改变,大肠癌的发病率呈逐年上升趋势.手术、放疗、化疗是大肠癌的主要治疗方法.近几年热疗已成为恶性肿瘤综合治疗的方法之一,可以通过致热效应和非致热效应杀伤肿瘤细胞,热疗不仅对肿瘤细胞有直接的治疗作用,而且热疔与放化疗联合应用还具有一定的协同作用.热疗联合传统疗法可以提高大肠癌局部控制率及患者生存率.本文就热疗的机制及国内热疗联合各种放化方法治疗大肠癌的疗效作如下综述.     

心理干预对肿瘤深部热疗患者恐惧心理的影响

目的探讨心理干预对肿瘤患者热疗前及热疗过程中恐惧心理的影响。方法将168例行热疗的中晚期恶性肿瘤患者随机分为干预组（96例）和对照组（72例）。对照组行常规治疗护理，干预组治疗前72h及治疗中增加心理干预。结果干预组治疗2个周期后恐惧程度显著轻于对照组（P〈0．01）。结论心理干预可减轻肿瘤患者对热疗的恐惧心理。     

热疗和LAK细胞联合对人胃癌细胞系的杀伤

浸润到浆膜层的胃癌患者，术后常由于腹膜上肿瘤脱落细胞种植而复发。本实验联合热疗和ＬＡＫ细胞作用于人胃癌细胞系ＳＧＣ－７９０１，结果表明对该种瘤细胞系的杀伤有协同作用，为胃癌患者术后腹腔内热疗和ＬＡＫ细胞联合应用提供了依据。．     

心理干预对肿瘤深部热疗患者恐惧心理的影响

目的探讨心理干预对肿瘤患者热疗前及热疗过程中恐惧心理的影响.方法将168例行热疗的中晚期恶性肿瘤患者随机分为干预组(96例)和对照组(72例),对照组行常规治疗护理,干预组治疗前72 h及治疗中增加心理干预.结果干预组治疗2个周期后恐惧程度显著轻于对照组(P＜0.01).结论心理干预可减轻肿瘤患者对热疗的恐惧心理.     

交变磁场感应肿瘤热疗设备的研究

随着热生物学、热生理学与电子技术的综合发展,肿瘤局部热疗成为近年国际肿瘤治疗的研究热点。肿瘤局部热疗(hyperthermia)希望能适形把癌瘤组织加热到46～70℃有效治疗温度范围,并维持一段时间,使癌细胞受到毁灭性的打击,并刺激机体免疫系统,增强机体对肿瘤的免疫力;同时要避免正常组织的过热损伤,减少患者不能忍受的各种创伤和威胁患者生命的并发症。与手术、放疗和化疗3种传统方法比较,肿瘤热疗处于辅助和次要地位,但有巨大开发和应用潜能。目前,用于肿瘤局部热疗的方法主要有射频加热、微波加热、超声波加热等[1],但这些方法各自存在一…     

微量元素对丝裂霉素C抗膀胱癌细胞生长的作用

为探讨微量元素对丝裂霉素Ｃ（ＭＭＣ）抗膀胱癌细胞生长的作用，采用体外细胞培养药物抑制实验方法对５份人膀胱移行细胞癌标本进行研究，结果：氯化镉以５００ｎｇ／ｍｌ浓度加入ＭＭＣ中时，其对膀胱癌细胞生长抑制５０％的浓度（ＩＣ５０）由单独用药时的１４．５ｎｇ／ｍｌ降至０．５６ｎｇ／ｍｌ，氯化镁以１０００ｎｇ／ｍｌ浓度加入ＭＭＣ中时，ＭＭＣ的ＩＣ５０降至１．９５ｎｇ／ｍｌ。而微量元素氯化镍及硫酸锂无此作用。     

射频组织间质热疗治疗腹腔肿瘤的研究现状

肿瘤热疗是近来肿瘤治疗学的一大热点,分为温热疗法和局部高温固化治疗。利用射频技术对腹腔深部肿瘤进行高温固化治疗是其中较有前途的方法之一,尤其在治疗肝脏肿瘤方面已取得了很大的进展。     

丝裂霉素对膀胱癌EJ细胞作用机制的研究

目的　从诱导坏死和凋亡角度探讨丝裂霉素 (MMC)对膀胱癌EJ细胞的作用机制。　方法　将不同浓度的MMC作用于膀胱癌EJ细胞 ,在不同时间内采用流式细胞术 (FCM )和 3’末端脱氧核苷酸转移介导生物素 (TUNEL)及伊红排斥实验检测EJ细胞的凋亡情况和对其的杀伤作用。　结果　不同浓度的MMC在 2 4h内可以持续诱导EJ细胞凋亡 ,MMC 0 .1mg/ml组 2 4h时凋亡率为 71% ,对EJ细胞的杀伤作用与剂量呈正比。　结论　MMC对膀胱癌EJ细胞的抑制作用是通过促凋亡机制和促坏死机制完成的     

The effect of hyperthermia on mitomycin-C induced cytotoxicity in four human bladder cancer cell lines

INTRODUCTION: Hyperthermia and mitomycin-C (MMC) have given very encouraging results in several clinical studies for the treatment of superficial transitional cell carcinoma of the bladder. However, a synergistic effect of hyperthermia and MMC on the decrease of cell proliferation has never been demonstrated accurately in vitro. We investigated the effect of MMC versus MMC combined with hyperthermia on the cytotoxicity in four human bladder cancer cell lines. MATERIAL AND METHODS: The RT112, RT4, 253J and T24 human bladder cancer cell lines were seeded in 96-well microtiter plates at 2.0 x 10(4) cells per well and were left to attach for 24 hours. The cells were then treated for 60 minutes with MMC concentrations ranging from 0 to 400 microg/ml at a temperature of 37 degrees C or 43 degrees C. After treatment cells were rinsed three times with culture medium and left for 24 hours in the incubator. Dimethyl thiazolyl tetrazolium (MTT) solution was added and after 4 hours of incubation the MTT containing media was aspired from all wells and 100 microl of dimethyl sulfoxide was added to each well. A spectrum analyses was performed at 595 nm light wavelength. RESULTS: A decrease of cell proliferation after treatment with increasing concentrations MMC was demonstrated. Hyperthermia has a synergistic effect on the decrease of cell proliferation by different concentrations MMC. In the cells treated without MMC no significant difference in the extent of cell killing at 37 degrees C and 43 degrees C was observed. Furthermore, no difference was observed between cells with a p53 protein mutation (RT112 and T24) or without a p53 protein mutation (253J and RT4). Conclusion: A clear synergistic effect of MMC and hyperthermia has been demonstrated in four human bladder cancer cell lines.     

丝列霉素处理的外周血单核细胞对大鼠同种异体移植心脏的影响

目的观察MMC处理的外周血单核细胞（PBMCs）对大鼠同种异体心脏移植的影响。方法BN大鼠作为供体，Lewis大鼠作为受体，体重在200～300g。PBMCs于含有MMC的细胞培养液中培养30min后于移植前1周静脉注射人受体，然后接受心脏移植，观察移植心脏的存活情况；并利用FACS检测MMC对PBMCs存活率的影响。结果MMC处理过的PBMCs诱导了细胞的凋亡；接受MMC处理过的PBMC的大鼠，移植心脏的存活时间明显得以延长。结论MMC处理的PBMCs可以延长大鼠移植心脏的存活时间，对移植心脏的延长作用可能是由于MMC诱导了细胞的凋亡引起的。     

The effects of hyperthermia on the cell cycle of Ehrlich ascites cancer cellsin vivo

The effects of hyperthermia on the cell cycle of Ehrlich ascites cancer cells were studied, and these effects simultaneously evaluated in terms of prolonging the survival of test mice inoculated with tumor cells from heat-treated mice. DDY mice bearing Ehrlich ascites cancer cells were placed in a water bath at 37°C, 39°C, 41°C, 32°C or 43°C. The heating of mice at 41°C, 42°C and 43°C induced the accumulation of cancer cells at the G₂M phase of the cell cycle with many cells exhibiting polyploidy (16C). The extent of accumulation increased as the temperature of incubation was raised, however the interrupted cell cycle resumed 120 hours after heating. The retransplantation of cells from the heat-treated mice revealed that the mice which were inoculated with Ehrlich ascites cancer cells from mice heated at 43°C survived longer, while the mice which were inoculated with Ehrlich ascites cancer cells from mice heated at 39°C survived for only a slightly shorter time than those which were inoculated with cells from mice heated at 37°C.     

不同浓度罗哌卡因对乳腺癌MCF-7细胞增殖和细胞周期的作用研究

目的观察不同浓度罗哌卡因对乳腺癌MCF-7细胞增殖和细胞周期的影响并探讨其机制。方法人乳腺癌细胞MCF-7接种于培养板培养24h,随机分为四组:对照组(C组)、罗哌卡因100μg/ml组(R1组)、罗哌卡因200μg/ml组(R2组)和罗哌卡因400μg/ml组(R3组)处理乳腺癌MCF-7细胞48h后,检测其细胞增殖能力(细胞活力)和细胞周期。检测R3组作用于MCF-7细胞48h后TCF-4、beta-catenin的蛋白表达水平。结果 R2、R3组MCF-7细胞活力明显低于C组(P0.05);R1、R2、R3组MCF-7细胞G0/G1期细胞明显少于C组,S期和G2/M期细胞明显多于C组(P0.05);R3组TCF-4和beta-catenin蛋白表达水平明显低于C组(P0.05)。结论罗哌卡因可能通过下调TCF-4和beta-catenin蛋白表达水平抑制人乳腺癌MCF-7细胞增殖。     

The anti-proliferative effect of proliferating cell nuclear antigen-specific antisense oligonucleotides on human gastric cancer cell lines

The proliferating cell nuclear antigen (PCNA) is a nuclear protein that leads DNA synthesis by the DNA polymerase delta. As the PCNA gene is strongly expressed in invasive gastric cancer cells with high proliferative activity, PCNA is suspected of playing an important role in the proliferation and advancement of gastric cancer. Thus, the effects of antisense oligonucleotides specific for PCNA mRNA were examined in seven gastric cancer cell lines. It was found that treatment with antisense oligonucleotides at concentrations of 10–40 M dose-dependently inhibited the growth of all cell lines; however, random sequence oligonucleotides did not modify the proliferation of any type of cells. These results indicate that PCNA is essential for cell proliferation in gastric cancer cells, and that the growth inhibitory effect results from the inhibition of PCNA gene expression. Therefore, PCNA-specific antisense oligonucleotides may be effective in the treatment of gastric cancer.     

The effect of hyperthermia on human prostatic carcinoma cell lines: evaluation in vitro

The effect of hyperthermia on established human prostate carcinoma cell lines (PC-3, DU-145) and related sublines (1-LN, 125-1L) was investigated in vitro. Cells were exposed to heat treatment at 43C or 37C for varying time intervals, (one hr or two hrs) and cell survival was evaluated by the colony formation assay and by measurement of cellular growth rate. While one hr exposure at 43C did show a mean inhibition of colony formation, ranging from 29 to 41%, a statistically significant increase in inhibition rate (p less than 0.001) was observed at two hr exposure, ranging from 57 to 92%. This study is a report of the cytotoxic effect of hyperthermia on established human prostatic tumor cell lines. These in vitro results indicate that hyperthermia may become a potentially useful form of adjunctive therapy for local control of prostatic cancer. However, the temperature and exposure time may have an important impact on cell kill when this new modality for cancer treatment is proposed for a clinical trial.     

胃癌肿瘤裂解物修饰的树突状细胞体外诱导抗原特异性细胞毒T淋巴细胞

目的　利用树突状细胞呈递肿瘤抗原的特性提高细胞毒T淋巴细胞 (CTLs)对胃癌细胞的杀伤活性。方法　胃细胞癌患者外周血来源的有核细胞体外经GM -CSF和IL -4诱导产生树突状细胞 ,负载肿瘤裂解物后诱导自体CTLs产生。用细胞毒试验检测CTLs杀伤活性 ,和用ELISA测定细胞因子的分泌。结果　胃癌患者自体来源的DC裂解物能诱导产生的CTLs对自体胃癌细胞具有高杀伤率 ,可达 83 % ;致敏的DC组中IL -12与TNF -α的浓度 ( 1161± 2 3 9pg/ml,10 44± 3 12 pg/ml)显著高于未致敏的DC组 ( P <0 .0 5 )。结论　DC能呈递胃癌裂解物 ,诱导产生抗原特异性CTLs。     

The presence of an aberrant type of human chorionic gonadotropin in patients with gastric or colorectal cancer

Beta subunits of human chorionic gonadotropin (β-hCG) and human chorionic gonadotropin-like substance (hCGLS) were measured radioimmunologically in the serum and malignant tissue from patients with gastrointestinal cancer. Since serum β-hCG and hCGLS correlate closely to those in cancer tissues, it is assumed that these two gonadotropins originate from cancer tissues. The serum hCGLS levels in 54 patients with gastrointestinal cancer were significantly higher, when compared with the findings in 19 healthy volunteers and 10 peptic ulcer patients. The frequency of high levels of serum hCGLS accounted for 71 per cent of those with operable gastric cancer, 44 per cent of those with inoperable gastric cancer, 100 per cent of those with operable colorectal cancer, and 67 per cent of those with inoperable colorectal cancer. On the contrary, serum β-hCG levels did not differ between the volunteers and the cancer patients. In the 17 sera and 15 cancer tissues assayed, β-hCG did not correlate to hCGLS. Moreover, the high levels of β-hCG in cancer patients occurred in only 1/14 (7.1 per cent) of the assayed serum, and in 5/14 (35.7 per cent) of the cancer tissue. The increased production of these two hCGs may result from neoplastic trans-fromation of an unrestrained fetal genome responsible for hCG production during gestation. It is assumed that the increased producibility of a defective hCG, i.e., an aberrant hCG such as hCGLS, is characteristic of malignant tumors.