真菌性角膜溃疡84例临床分析

目的总结分析真菌性角膜溃疡的临床治疗效果。方法采用抗真菌药物及3％碘酊烧灼角膜溃疡面,清除病灶坏死组织等治疗。结果治愈72例（占85％）,好转10例（占10．9％）,2例角膜穿孔（占2．38）。结论抗真菌药物联合3％碘酊烧灼角膜溃疡面,清除病灶坏死组织,促进角膜愈合等治疗,病程明显缩短,疗效明显提高。     

氟康唑联合碘酊烧灼治疗真菌性角膜溃疡临床分析

目的分析氟康唑联合碘酊烧灼治疗真菌性角膜溃疡治疗效果。方法通过对角膜溃疡病灶清除,5%碘酊烧灼联合氟康唑治疗24例。结果有18例治愈,治愈率75.0%。结论氟康唑联合碘酊烧灼治疗真菌性角膜溃疡效果明显。     

角膜病灶表面清创术、碘酊烧灼联合自制氟康唑粉剂、透明质酸钠眼用凝胶治疗真菌性角膜溃疡的疗效

目的观察角膜病灶表面清创术、碘酊烧灼联合自制氟康唑粉剂、透明质酸钠眼用凝胶敷眼治疗真菌性角膜溃疡的临床疗效。方法对52例(52只眼)真菌性角膜溃疡患者行角膜病灶表面清创术、碘酊烧灼联合自制氟康唑粉剂、透明质酸钠眼用凝胶敷眼治疗。结果 52例(52只眼)患者中,痊愈46例(46只眼);好转3例(3只眼);无效3例(3只眼),其中2例(2只眼)转上级医院行角膜移植术,1例(1只眼)行眼内容物剜除术。结论角膜病灶表面清创术、碘酊烧灼联合自制氟康唑粉剂、透明质酸钠眼用凝胶敷眼治疗真菌性角膜溃疡安全、有效。     

碘酊烧灼联合氟康唑治疗真菌性角膜溃疡36例

目的 探讨碘酊烧灼联合氟康唑治疗真菌性角膜溃疡的疗效.方法 对角膜溃疡面进行清创,5%碘酊小病灶早期烧灼,或大病灶的中晚期烧灼,一般不超过三次.氟康唑结膜下注射,加频点眼,加强散瞳,素高捷眼膏涂患眼.重症患者加服消炎痛.结果 36例(36只眼)治疗9～30天,痊愈32例,有效2例,无效2例,总有效率94.44%.结论 碘酊烧灼联合氟康唑治疗真菌性角膜溃疡取得满意效果,值得推广.     

氟康唑联合碘酊治疗真菌性角膜溃疡42例临床分析

目的探讨氟康唑联合碘酊治疗真菌性角膜溃疡的效果。方法对42例真菌性角膜溃疡应用氟康唑点滴患眼或结膜下注射,碘酊烧灼角膜病灶等治疗,观察其效果。结果32例治愈,8例好转,视力有所提高;2例未愈。结论应用氟康唑联合碘酊治疗真菌性角膜溃疡疗效显著。     

真菌性角膜溃疡综合治疗的临床观察

目的探讨真菌性角膜溃疡综合治疗的疗效.方法52例52眼真菌性角膜溃疡分为两组,治疗A组35例应用氟康唑、角膜病灶局部清创及碘酊烧灼综合治疗,治疗B组17例单纯应用氟康唑治疗,对比两组治疗效果.结果综合治疗效果明显优于单纯药物治疗.结论广谱抗真菌药物氟康唑联合局部清创及碘酊烧灼的综合治疗方法治疗真菌性角膜溃疡的疗效显著.     

特比奈芬联合5%碘酊治疗真菌性角膜溃疡

目的 观察特比萘芬联合5%碘酊治疗真菌性角膜溃疡的疗效.方法 临床确诊为真菌性角膜溃疡32例(32眼),特比奈芬口服联合用5%碘酊点眼,治疗真菌性角膜溃疡32例.结果 随访3个月,32例中25例角膜溃疡愈合,好转4例,无效3例.结论 特比萘芬联合5%碘酊治疗真菌性角膜溃疡疗效好,无明显副作用.     

碘酊烧灼联合氟康唑治疗真菌性角膜炎疗效观察

目的 探讨碘酊烧灼联合氟康唑滴眼治疗真菌性角膜炎的疗效.方法 对36例真菌性角膜炎的患者采取碘酊烧灼联合氟康唑滴眼治疗,观察治疗效果.结果 经过碘酊烧灼联合氟康唑滴眼治疗,轻度患者治愈率100%,中度者66.6%,重度者33.3%.结论 碘酊烧灼联合氟康唑滴眼对轻、中度真菌角膜溃疡治疗效果好,对重度者效果欠佳.     

碘酊烧灼联合贝复舒加口服斯皮仁诺治疗真菌性角膜炎

目的：观察碘酊烧灼联合贝复舒加口服斯皮仁诺治疗真菌性角膜炎的临床效果。方法：对11例11眼经角膜病灶区组织刮片镜检或分泌物涂片行镜检，确认为真菌性角膜炎，局部用10％碘酊烧灼联合贝复舒加口服斯皮仁诺治疗。结果：11例11眼经治疗8例治愈，3例有效。无1例无效。结论：真菌性角膜炎在早期及时确诊后，局部用碘酊烧灼联合贝复舒加口服斯皮仁诺治疗，取得较好结果。     

真菌性角膜溃疡68例临床分析

目的分析真菌性角膜溃疡的临床表现及综合治疗效果。方法对68例真菌性角膜溃疡在抗真菌进行药物治疗的基础上，应用病灶刮除、生理盐水冲洗、2．5％碘酊烧灼创面、结膜瓣覆盖等方法综合治疗，观察病变角膜的改变。结果治愈57例（83．8％），显效6例（8．8％），有效2例（2．9％），无效3例（4．4％），总有效率95．6％。结论对真菌性角膜溃疡以药物治疗为主，辅以其它综合治疗是安全和有效的。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.