在成分血生产过程中制备少白细胞浓缩血小板

用含CPD—1保养液的三联袋采集1单位全血于第一袋中,以2200rpm 离心5分钟,加速时间为1分钟,减速过程为3分钟,此时全血为压缩红细胞(RBCs)和富含血小板血浆(PRP),二部分加压使PRP 通过去除白细胞的滤器(由表面处理过的聚酯微纤维制备)进入血小板收藏袋(第二袋),即得少白细胞PRP.再以3250rpm 离心15分钟,加速时间为1.6分钟,降速时间为3.5分钟,然后将上层少血小板血浆挤入第三袋作为新鲜冰冻血浆使用,在第二袋中留下50—60ml 即为少白细胞浓缩血小板。用此方法共制备15份少白细胞浓缩血小板,平均体积为56.7ml.第5天血小板含量平均为8.6×10~(10)/单位。第5天白细胞含量平均为0.83±0.7×10~4/单位(0.14白细胞/μl),而对照组14例,白细胞平均含量为5.95±2.86×10~7/单位,白细胞去除率99.9%。11例配对比较,样本组血小板为7.3±1.4×10~(10)/单位,而对照组为7.7±1.5×10~(10)/单位,血     

白细胞含量对血小板保存条件的影响

为评价浓缩血小板(PC)中白细胞含量对血小板保存条件的影响,作者以CPD为抗凝剂收集了12个单位全血,分别制备成PC后,将其混合,重新分成12份。然后将这12份相同的PC分成4组(Ⅰ-Ⅳ组),每组3份,分别加入不同量的富含白细胞血浆和少白细胞血浆,所有操作在无菌条件下进行。所得PC的血浆体积为58.6±0.6ml,血小板浓度为1.01±0.04×10~9/ml,所含红细胞数小于10~7。Ⅰ-Ⅳ组PC中白细胞的浓度分别为0.14±0.05,1.96±0.09,5.53±0.98和13.00±0.93×10~6/ml。将制备好的上述PC装于普通聚氯乙烯塑料袋内置22℃保存7天,分别于0、2、5、7天时取样测定血小极性质的变     

贮存前除去白细胞对贮存血小板浓缩物细胞因子水平的影响

作者用6份富含血小板血浆(PRP)分别制成不除去白细胞的血小板浓缩物(PC)和用过滤法除去白细胞的PC,对另外8份PC应用血沉棕黄层(Bc)法除去白细胞。分别在贮存前、贮存第3天和第5天,测定各组TNF-α、IL-1β和IL-6的含量变化。 结果:过滤法的PC组,白细胞除去率为96.2±4.1％,PC中白细胞数为0.22±0.29×10~9/L,与     

用前到腺素E_1制备的浓缩血小板在体内的恢复与存活

贮存5-7天的浓缩血小板(PC)在输注后恢复和存活不如新鲜血小板。作者首先在新鲜富血小板血浆(PRP)中加入一种生化激活剂后进行制备,并与常规PC制备法对照。方法:采集至少10天未服药的男性献血者全血,枸橼酸-磷酸-葡萄糖(CPD)抗凝,室温离心(1000g,7分钟)制备PRP。加入用正常盐水稀释的前列腺素E_1(PGE_1)使其最终浓度为1.3×10(~-8)M,然后室温3500g离心7分钟制备PC。PC于22℃±1℃贮存5天后,在使用前2-3小时以自身血浆浸泡血小板,然后弃液体,计数血小板、白细胞,测定pH,按Kunickl法作形态学积分,每个单位浓缩血小板加入250μCi铬酸钠(~(51)Cr)标记,22℃孵育30分钟,以自身血浆洗涤后配成30ml悬液,于输入后1.5、2.0、24、     

单采血小板保存7天:体外研究报道

背景 这项研究的目的是确定单采血小板在血小板保存袋(COBE ELP,Gambro BCT)中保存7天的变化。方法 每袋可控制浓度和容量的单采血小板在血浆中22℃水平摇动保存到8天。体外常规检测血小板参数。耗氧量采用克拉克电极直接检测。所有血小板在第7天作有氧培养。结果 检测了24单位保存状态下的血小板(中位数(范围):340[110-420]ml,1.32[0.99-2.45]×10~6血小板/μl,和每袋有4.8[1.4-5.9]×10~(11)血小     

普通血袋常温保存分离后富含血小板血浆24 h再制备浓缩血小板的质量观察

目的探讨普通血袋常温分离富含血小板血浆(PRP)后保存24 h再制备的浓缩血小板(PC)质量。方法将采集的400 mL全血50袋,保存在20-24℃的恒温保存箱内,采集后4 h经1次轻离心分离出PRP,容量(200±10)mL/份,再每份均分成2袋存放于普通血袋中,分别标识为实验组:将PRP放置20-24℃的恒温保存箱内过夜,次日2次重离心,分出上清血浆,制备成PC,从全血采集时间算起,24 h完成制备;对照组:将PRP立即2次重离心,分出上清血浆,制备成PC,从全血采集时间算起,6 h完成制备。采集后24和48 h,分别检测2组PC中血小板、红细胞含量和pH值。结果 2组PC中红细胞混入量和pH值差异甚小(P0.05),实验组与对照组血小板含量(×1010/袋)分别为1.36±0.33和2.63±0.46(P0.05);对照组3项指标均合格,实验组只有红细胞混入量与pH值合格。结论分离PRP后,在普通血袋常温保存24 h再制备浓缩血小板,质量达不到《全血与成分血质量要求》。     

用混合白膜制备血小板浓缩物:使用自动OrbiSac系统的体外研究和经验

背景本研究的目的在于评估自动OrbiSac系统用混合白膜(BCs)制备保存于血小板添加液中的血小板浓缩物(PCs) 的质量。设计和方法实验1是制备PCs的配对研究(6份 BCs),使用自动和手工程序。实验2和3是评估OrbiSac系统制备的PCs(6份BCs),实验3包括依据捐献者的数据选择 BCs。实验4是配对体外试验,使用了白细胞过滤器和两种不同的保存容器。实验5评价连接有白细胞过滤器的OrbiSac系统制备的PCs(6份BCs)。实验6与实验5类似,使用的是计算机选定的混合5份BCs。体外研究还评估了7天保存对血小板代谢和降解。结果实验1和4有类似的体外实验效果。在实验 2中,血小板浓度为370×109±70×109／PC,BCs的回收率为 76±6％。在实验3中,血小板的浓度为380×109±50×109／ PC,与随机选取BCs混合相比,变异程度较小。在实验5中,血小板浓度有所增加(420×109±70×109／PC,BCs的回收率为 80±5％)。在实验6中,5份BCS制备的血小板为340×109± 60×109／PC,回收率为79±5％。结论这些体外研究表明,保存7天的的血小板体外特性方面,OrbiSac,技术与标准的手工方法制备的血小板的质量相似。依据血液捐献者血小板计数选择BCs混合,制备标准化的血小板计数的PCs,其结果是令人鼓舞的。     

白膜放置时间对制备手工浓缩血小板质量的影响

目的评价全血当天分离的白膜(BC)在不同时间分离制备的浓缩血小板(PC)的质量,为手工制备PC提供参考。方法将80袋400 mL全血于采集6 h内分离出BC,将5袋同血型BC由无菌接驳机对接合并成1袋(1个治疗量)后,再均分在3个血小板保存袋内,1袋即刻(0 h组)轻离心分离制备PC,另2袋在22℃血小板保存箱分别振摇4 h(4 h组)和16 h(16 h组)后再分离PC。对所有标本留样进行血小板质量检测,包括Plt、血小板回收率、CD62P、聚集率、RBC混入量、WBC混入量、FHb含量。结果 3组PC制剂RBC混入量、Plt、血小板回收率、CD62P、聚集率差异无统计学意义;WBC混入量:0 h(6.76±1.29)和4 h组变化不明显,16 h(3.78±0.45)组降低明显(P<0.05);FHb含量:随BC处理时间延长有增高趋势,16 h(65.62±11.11)与0 h(33.45±6.95)比差异具统计学意义(P<0.05)。结论随BC放置时间延长对制备PC制剂的质量有一定影响。     

离心制备无白细胞的血小板浓缩物

反复输注随机供血者的血小板常引起受体抗血小板输注,最近的研究认为这一现象并不是由血小板引起,而是由血小板中污染的白细胞所致.本文报道用标准血小板浓缩物制备无白细胞污染的血小板的离心技术.3单位血小板浓缩物在一转移袋内混和(150ml),在以下条件室温离心:200×g,10分钟;300×g,5分钟;300×g,10分钟;370×g,5分钟.吸出上清液,袋内留约30ml 血浆。离心前后,对每袋混合浓缩物作白细胞和血小板计数,根据离心后血小板浓度     

比较三种用于混合血奶黄层制备的血小板的WBC的滤器:血小板的活化和微囊泡化

尚不知道不同的WBC滤器过滤由混合血奶黄层(BC)所制备的浓缩血小板(PC)是产生还是消除了微泡(MV)。此外,由每种滤器所提供的各种保存袋可能会对血小板的微囊泡化产生不同影响。作者调查了3种内置式滤器/血袋,即PL×5/PL2410、Autoshop BC/CLX和Imugard Ⅲ/Teruflex。在每组试验中,4单位混合BC来自同一献血者。采用这3种滤器中之一种过滤由每一份混合BC制得的PC,第4份混合BC则采用CLX袋、不过滤作为对照。每单位25ml血液保存于Cobe样品袋以消除保存袋间的差异性。所有过滤的PC中WBC<1×10~6/单位。由这四组所测得的容量、血小板产量和pH相当。血小板膜(m)和上清     

Collection of platelets and peripheral progenitor cells with the Fresenius AS.TEC 204 blood cell separator

The aim of the present study was to clinically evaluate the blood cell separator AS. TEC 204. One hundred fifteen platelet collections were carried out with the dual or single needle procedure. Platelet yield was 3.21 ± 0.80 × 10¹¹ (mean ± standard deviation) and 59.1% of the collections showed platelet counts >3.0 × 10¹¹. Leukocyte contamination was 1.77 ± 2.81 × 10⁶ and 89.0% of the platelet concentrates had a white blood cell content <5 × 10⁶. Using a dual needle technique with an alternating interface adjustment, all of the products were contaminated with less than 1 × 10⁶ leukocytes. Furthermore, 23 peripheral progenitor cell collections were performed in 12 patients and three allogeneic donors. Median numbers of harvested CD 34 antigen expressing cells/kg body weight were 0.78 (range 0–4.7) and 3.67 × 10⁶ (range 2.2–5.23), respectively. We conclude that platelet and progenitor cell collections can be carried out with efficient results. The collections were well tolerated by the donors. J. Clin. Apheresis 12:126–129, 1997. © 1997 Wiley-Liss, Inc.     

PLT-30: An improvement for platelet collection on the baxter CS-3000 plus

The CS-3000 is a well-established blood cell separator. To increase the efficiency of platelet collection, Baxter developed a new separation chamber, the TNX-6, for the follow-up model of the CS-3000, the CS-3000 Plus. To reduce the occurrence of platelet aggregates and to increase platelet yield and efficiency the collection chamber A-35 has been replaced by a new, volume-reduced chamber called PLT-30. We analyzed on the basis of 201 platelet separation procedures with the CS-3000 Plus fitted with both new chambers the efficiency of platelet collection. The platelet yield per unit (mean ± SE) was 4.55 × 10¹¹ M-1 0.07 × 10¹¹ and the efficiency was 50.79 ± 0.76%; only 3.98% of the platelet concentrates were below 3 × 10. To compare our data with results from the A-35 we enrolled 33 random donors (17 in Innsbruck, 16 in Vienna) in a controlled study. Using both chambers in a randomly selected sequence within a period of 1 month on each chamber, we evaluated platelet yield, efficiency, and the contamination with white blood cells (WBCs). We found a platelet yield per unit (mean ± SE) of 3.74 × 10n ±0.11 × 10¹¹ using the A-35 and of 4.4 × 10¹¹ ±0.21 × 10¹¹ with the new PLT-30 (P = .0001). The efficiency of the collecting process (mean ± SE) was higher with the PLT-30 (47.76 ±2.11 %)than with the A-35 (40.68 ± 1.29%). The difference is statistically significant (P= .0001). Comparing the contamination of the platelet concentrates with WBCs no significant differences could be found (P= .35). Our results demonstrate that the use of the new collection chamber PLT-30 in combination with the separation chamber TNX-6 significantly increases platelet yield and efficiency per unit, while no decrease of the contaminating WBCs could be obtained.     

改良白膜法制备浓缩血小板及回收率的影响因素

背景：白膜法和富含血浆法制备的浓缩血小板有无效输注发生率高和不良反应发生率高的缺点。 目的：观察改良白膜法制备浓缩血小板的实验研究,分析制备浓缩血小板回收率的影响因素。 方法：随机抽取126例站内采集后4-6 h的400 mL血液,随机分成改良白膜法组、白膜法组和富含血浆法组。改良白膜法采用3步离心,第1次采用次重离心,离心转速2300 r/min,离心时间12 min,降速5,离心温度（22±2）℃;第2次采用轻离心,离心转速910 r/min,离心时间10 min,离心温度（22±2）℃;第3次离心转速2800 r/min,离心时间12 min,离心温度（22±2）℃,离心后,挤去上层含血小板较少的血浆,袋中留30 mL血浆悬浮血小板,即为浓缩血小板。通过数据库文献检索的方法分析制备浓缩血小板回收率的影响因素。 结果与结论：改良白膜法、白膜法以及富含血浆法制备的手工浓缩血小板中,制备前各组血小板总数差别无统计学意义（P 〉0.05）;富含血浆法组和改良白膜法组较白膜法组血小板回收率高,差异有显著性意义（P 0.05）;白膜法组和改良白膜法组较富含血浆法组残留红细胞和残留白细胞的量少,差异有显著性意义（P0.05）。制备浓缩血小板的回收率受到全血量、离心转速、离心时间、离心方法等因素的影响。改良白膜法制备浓缩血小板减少红细胞和白细胞的残留量,提高了血小板的回收率,可在血液中心或中心血站推广应用。     

Factors influencing white cell removal from red cell concentrates by filtration

BACKGROUND: The preparation of blood components by hard centrifugation results in red cell concentrates with a small amount of plasma. The influence of various plasma factors, temperature, and storage time on white cell reduction by filtration was studied. STUDY DESIGN AND METHODS: Red cell concentrates were suspended in 100 mL of saline- adenine-glucose-mannitol (SAGMAN) solution or in SAGMAN solution in which 5 or 10 mL had been replaced with an equal amount of fresh plasma, albumin (4%), or heat-inactivated plasma. After overnight storage at 4 degrees C, filtration at a slow flow rate (2 hours) was performed. The effect of temperature was studied by filtration at 4 degrees C and 37 degrees C. To study the influence of storage time, red cell concentrates were stored for 4 to 8 hours or 14 to 20 hours at 4 degrees C and filtered through another model of filter. The number of white cells was counted microscopically or by flow cytometry. RESULTS: When 5 or 10 mL of plasma was added, a significantly smaller number of white cells were found after filtration than were found in the SAGMAN control (the median difference between pairs: 23.6 × 10(6) for 5 mL [p = 0.006] and 14.9 × 10(6) for 10 mL [p = 0.003]). The number of white cells was significantly higher with 10 mL of albumin than with 10 mL of plasma (difference, 15.0 × 10(6); p = 0.006). When heat-inactivated plasma was used, the number of white cells was significantly lower than when fresh plasma was used (difference, 0.3 × 10(6); p = 0.009). Filtration at 37 degrees C resulted in a 64-percent reduction in white cells and that at 4 degrees C led to a 99.7-percent reduction (p = 0.006). When the second filter was used, a slight but significantly lower number of white cells was found in the red cell concentrate stored for 14 to 20 hours than in that stored for 4 to 8 hours (difference, 0.03 × 10(6); p < 0.001). CONCLUSION: The amount of plasma in the red cell concentrate and the storage time and temperature are important factors in the outcome of white cell reduction by filtration. The effect of plasma does not seem to be due to a general influence of protein or to the activity of complement or fibrinogen.     

Leukotrap,a device for white cell poor platelets quality control studies In vitro and In vivo

Most febrile transfusion reactions are due to leucoagglutinins. Cutter's Leukotrap platelet pooling bag has a distal conic pouch for depleting the platelets of white blood cells by centrifugation. We tested 33 Leukotraps each containing six platelet units in vitro and 32 in vivo. The mean in vitro platelet count was 3.7 ± 0.5 × 10¹¹ platelets before, and 3.0 ± 0.5 × 10¹¹ after spinning, representing a platelet recovery of 80.2 ± 9.6% Mean white blood cells were 3.8 ± 0.6 × 10⁸ before, and 0.6 ± 0.1 × 10⁸ after centrifugation, this constituting a white cell removal of 83.5 ± 7.7%. pH ranged from 7.37 for 24-h platelets to 7.19 for 96-h platelets. 24-h after platelet pooling, all Leukotraps were sterile. Platelet aggregation with physiologic agents showed little change compared to individual platelet units. Glucose ranged between 418 and 336 mg/dL, pCO₂ between 27.8 and 19.1 mmHg, but pO₂ dropped drastically from 74.8 mmHg to 11.6 mmHg. Hypotonic osmotic recovery was satisfactory. In vivo studies were carried out with pooled, leucocyte-poor platelets which were transfused to six bone marrow transplant patients with no splenomegaly or septicemia at the outset. These patients had all demonstrated febrile transfusion reactions to standard donor units. The mean platelet increment was 16.8 × 10⁹/L. A single febrile transfusion reaction witnessed in one patient, was accompanied by an adequate platelet response. Hence Leukotrap is a useful clinical tool for reducing febrile transfusion reactions related to white blood cells.     

In vitro and in vivo effects of prestorage filtration of apheresis platelets

BACKGROUND: The effect of prestorage filtration on the quality of apheresis platelet concentrates stored for transfusion is undetermined. STUDY DESIGN AND METHODS: Investigation of 11 plateletpheresis components used a concurrent paired-study design. On the day of collection, each component was equally divided into two suspensions; one half was filtered, and the other half was not. Each suspension was stored for 5 days. In vitro testing was performed on the day of collection (Day 0) for cell counts and on Day 5 for measurements of lactate, glucose, blood gases, pH, platelet ATP, hypotonic stress ratio, extent of shape change in response to ADP, tissue necrosis factor alpha, interleukin 8, interleukin 1 alpha, interleukin 1 beta, interleukin 6, and platelet surface glycoproteins by flow cytometry. At the end of the 5-day period, a sample was taken from each of the two suspensions, radiolabeled with either 51Cr or 111In, and transfused concurrently. Posttransfusion samples were drawn for measurements of recovery and platelet survival and for functional assessment of the ex vivo ability of the circulating radiolabeled platelets to aggregate in response to ADP. RESULTS: The apheresis component had a mean platelet yield of 3.2 +/? 0.4 × 10(11) and a white cell yield ranging from 1 × 10(5) to 1 × 10(8), with a median of 2 × 10(7). Filtration resulted in a platelet loss of approximately 10 percent and a variable 2 to 3 log10 reduction in white cell content. No significant differences between filtered and unfiltered suspensions in paired t tests that would likely have an impact on platelet quality were observed in the in vitro tests. The in vivo recovery and survival were highly similar and not statistically different in filtered and unfiltered paired suspensions: the mean difference was 1.2 +/? 4.0 percent for recovery and 7.0 +/? 15 hours for survival. The functional assessment by aggregation to ADP showed no difference between filtered and unfiltered suspensions. A small decrease in tumor necrosis factor alpha and interleukin 8 was evident in the filtered suspension as compared to levels in the unfiltered suspensions. CONCLUSION: Prestorage white cell reduction in apheresis components resulted in WBC reduction by several log10 with no evident adverse effect on platelet viability or function.     

白细胞过滤对血小板保存期间炎性细胞因子水平的影响及临床效果的评估

目的　考察浓缩血小板悬液 (plateletconcentratesuspend ,PCs)在保存期内IL 1β、IL 6、IL 8和TNF α的浓度变化和过滤对其的影响 ,了解在保存前滤除PCs中的白细胞是否能有效地减少这些细胞因子的积累和降低受血者非溶血性发热性输血反应 (febrilenonhemolytictransfusionreactions,FNHTR)发生率。 方法　将 1单位PCs分成两等份 ,分别给予血小板专用白细胞滤器过滤处理和不滤除白细胞处理 ,保存 5d。在 0、3、5d测定IL 1β、IL 6、IL 8和TNF α含量及白细胞计数 ,采用配对t检验进行统计分析 ;临床观察未滤组和过滤组PCs输后FNHTR发生率。结果　PCs中的白细胞计数与保存 5d时IL 1β、IL 6、IL 8和TNF α水平之间呈正相关。未滤组PCs中有较多白细胞混入 [(35 1± 81)× 10 6/袋 ],在保存期间IL 1β、IL 6、IL 8和TNF α水平明显升高 ;过滤组的PCs残余白细胞 <1× 10 6/袋在保存期间诸细胞因子均保持在 0d水平 ;临床观察显示 ,末滤PCs与过滤PCs输注后FNHTR发生率分别为 2 0 .83%和 5 .83% ,P <0 .0 1。结论　保存前用血小板专用去白细胞滤器去除PCs中残留的白细胞能有效地防止细胞因子的积累 ,同时保留 95 %以上的血小板。输注滤除白细胞的PCs能有效地减少FNHTR发生     

Prevention of platelet alloimmunization

Among the immunological problems met within platelet transfusion therapy, HLA alloimmunization represents a major, if not the major risk: the management of alloimmunized patients represents a difficult challenge, inciting clinicians and blood bankers to propose and apply several methods for preventing platelet alloimmunization.Among all the methods published in the literature and detailed in this paper, two are of particular interest:

• 1.(1) the use of leukocyte-poor blood components with less than 1 × 10⁶ leukocytes per unit;
• 2.(2) the irradiation of platelet concentrates in UVB permeable plastic bags.

     

Large platelets continue to circulate in an activated state after myocardial infarction

Abstract This study was intended to investigate the actual platelet activation status after an acute coronary event. The activation status of circulating platelets was assayed directly by measuring the membrane activation markers CD62 and CD63 with the Düssel-dorf III flow cytometry test in 22 patients with the diagnosis of acute myocardial infarction during the 48-h observation period following the acute event. The number of activated, marker-positive sample platelets was significantly increased in the post-MI patients: CD62: 5·8%× 2·25^±1 vs. 3·5%× 2·32^±1, P≤ 0·05; CD63: 18·7%× 1·77^±1 vs. 4·6%× 2·16^±1, P≤ 0·00·1. The platelet volume and count were concomitantly increased (12·1 ± 2·4 fl/ 236 ± 90 times 10³μl^-1 compared to 8·3 ± 1·6 fl/187 ± 42 times 10³μl^-1) in the control group. Particularly large platelets were identified as being activated documented by the exponential increase in the difference in CD63-binding sites per sample platelet above the 90%-percentile and below the 10%-percentile of the volume distribution: Δ+ 1341 ± 903 (MI patients) vs. Δ+ 276 ± 126 (controls), P≤ 0·00·1. Significant creatine kinase elevation and decrease in platelet count was found in the non-survivor subset (n= 5). We conclude that predominantly large platelets continue to circulate in an activated state after MI. This study provides direct evidence that the assumption of an increased thrombotic potential becomes operative in vivo in MI patients. Besides CK elevation and decrease in platelet count this might possibly constitute a prognostic factor for the short-term outcome of the patients.     

Improvement of platelet storage conditions by using new polyolefin containers

BACKGROUND : Storage of pooled platelet concentrates (PCs) with yields above 3.0 × 10¹¹ platelets per unit in a 1-L PL-732 polyolefin container for 5 days often results in a drop in pH to below 6.0. Recently, new oxygen-permeable platelet containers (1-L PL-2410, 1-L and 1.5-L Compoflex) have been developed. The maximal platelet storage capacities of the new containers and the PL-732 were compared. STUDY DESIGN AND METHODS : Large platelet pools (n = 27) with platelet concentrations between 1.2 and 1.4 × 10¹¹ per L were made from 3 to 5 PCs prepared from buffy coats. The pools were divided in equal volumes among the PL-732 and the three new platelet containers. Platelet counts in the PCs ranged from 1.0 to 5.0 × 10¹¹ per unit. All PCs were stored on a flatbed shaker at 22 ± 2°C and evaluated on Days 1, 3, 5, and 7 by measuring platelet count, pH, pO₂, pCO₂, HCO₃-, glucose, lactate, platelet swirling, and soluble p-selectin. RESULTS : Day 7 storage of PCs (n = 6) with yields between 3.0 and 4.0 × 10¹¹ platelets in PL-732 showed mean ± SD pH values of 5.93 ± 0.05 and lactate values of 32.3 ± 7.9 mmol per L; in 4 of these 6 PCs, pH was below 6.0. In contrast, storage of these PCs in 1-L PL-2410 and 1.5-L Compoflex containers and of 2 of these 6 PCs in 1-L Compoflex containers showed pH values above 6.8. Lactate values were 15.5 ± 1.3, 15.3 ± 1.8, and 19.5 ± 4.7 mmol per L, respectively (p < 0.001 vs. PL-732). The platelet storage capacity of the new containers with platelet yields between 4.0 and 5.0 × 10¹¹ per unit (n = 6) was evaluated. Day 7 storage of these PCs in the 1.5-L Compoflex showed an average pH value of 6.74 ± 0.20; in 2 of 6 PCs, pH was below 6.8. The average pH value in the PL-2410 was 6.38 ± 0.31, and in all PCs, pH was below 6.8. Average lactate values were 17.8 ± 5.7 and 25.8 ± 5.6 mmol per L (p < 0.05), respectively. Soluble p-selectin values on Day 7 of storage increased approximately twofold in all PCs. CONCLUSION : The new oxygen-permeable containers showed platelet quality comparable to that with the PL-732 and for longer storage periods and at higher platelet counts.