喉癌及鼻腔鼻窦癌患者淋巴细胞染色体不稳定性的研究

本实验用TC199培养基,对37例喉癌、鼻腔鼻窦癌和20例正常人外周血淋巴细胞的染色体不稳定性进行了研究。结果表明,两种癌症患者淋巴细胞自发的染色体畸变率、微核率及平阳霉素(博莱霉素)诱发的染色体畸变率,微核率分别是11.73%、23.68‰、23.97%及47.16‰;对照组正常人淋巴细胞自发的染色体畸变率,微核率及平阳霉素诱发的染色体畸变率,微核率分别是1.95%、6.15‰、5.45%及15.85‰。两组上述各率彼此相比差异均有显著性(P<0.01)。提示喉癌及鼻腔鼻窦癌患者的淋巴细胞存在着染色体不稳定性。     

肿瘤病人及正常人外周血大颗粒淋巴细胞的观察

本文报道了LGL细胞在肿瘤病人外周血中的数量;与治疗有关的动态变化及正常人LGL细胞与NK活性的关系。发现除了LGL细胞具有颗粒外,少数小淋巴细胞浆内也具有颗粒。19例癌症治疗好转病人LGL细胞百分数较正常人为高。18例癌症恢复健康病人LGL细胞百分数与正常人相比无明显差异。5例脑肿瘤手术后及9例肝癌住院治疗病人LGL细胞升高。20例正常人LGL数量与NK活性之间有平行关系。     

非Graves甲亢和Graves'病FT3、FT4、TSH、TGA、TPO-Ab的变化及意义

目的探讨FT3、FT4、s-TSH(超灵敏)、甲状腺过氧化物酶抗体(antithyroid peroxidase antibody, TPO-Ab)、抗甲状腺球蛋白抗体(antithyroidglubulin antibody, TGA)水平在甲亢疾病和Graves'病中的改变及其临床意义.方法对43例非Graves'病患者和29例Graves'病患者、40例正常人分别检测血清TF3、FT4、s-TSH、TPO-Ab、TGA的水平.结果非Graves'病甲亢患者的FT3(15.01±11.01pg/ml)、FT4(38.30±19.82pg/ml)均明显高于正常人(分别为4.27±0.73pg/ml,16.16±2.58pg/ml,P＜0.01),s-TSH(0.25±0.25mIU/L)明显低于正常人(2.24±1.26mIU/L,P＜0.01),TPO-Ab(1319.74±1037.78IU/ml)明显高于正常人(0.01±0.01,P＜0.01)、TGA(33.39±22.43%)明显高于正常人(6.76±2.01,P＜0.01).Graves'病患者FT3(15.23±9.57pg/ml)、FT4(38.57±17.39pg/ml)均明显高于正常人(P＜0.01),s-TSH(0.79±0.46mIU/L)明显低于正常人(P＜0.01),TPO-Ab(2023.24±621.00IU/ml)明显高于正常人(P＜0.01)、TGA(49.72±20.55)明显高于正常人(P＜0.01),甲亢患者和Graves'病患者FT3、FT4、s-TSH无明显差别,但TGA和TPO-Ab明显低于Graves'病患者(P＜0.05).结论FT3、FT4、s-TSH(超灵敏)、甲状腺过氧化物酶抗体(TPO-Ab)、抗甲状腺球蛋白抗体(TGA)在判断甲亢和Graves'病患者中有重要的临床鉴别诊断意义.     

免疫缺陷病与恶性肿瘤

原发性免疫缺陷病伴发恶性肿瘤原发性免疫缺陷病患者中的癌症病死率超过一般人群100～200倍。原发性免疫缺陷病患者中恶性肿瘤发病率各家报导不一,从1.2%(700例)到1.7%     

聚蔗糖代替小牛血清检测E花环及其临床应用

<正> 本文介绍用10％聚蔗糖代替小牛血清行E-玫瑰花环试验,并经70例正常人,60例肺结核病人和80例肝脏病患者对比测定,其结果如下。 一、用5％、10％、20％聚蔗糖液和小牛血清同时对70例正常人进行E-玫瑰花环对比试验,结果见表1。     

肿瘤病人的血清C反应性蛋白(CRP)含量分析

应用双抗元辨认的抗CRP单克隆抗体E-LISA法测定了152例肿瘤病人和67例正常人血清CRP浓度,结果肿瘤病人明显高于正常人的3.8μg/ml±1.6的血清水平。152例癌症病人中。肝癌87例、肺癌40例、胃癌28例、直肠癌17例,各种癌症中又依据带瘤     

HLA抗原与Graves病关联的研究

Graves 病是一种自身免疫性疾病,确切发病机理未明,可有明显的家族遗传性。本文调查了本地区61例Graves 病患者和39例正常人的HLA-A、B、C_w 抗原分型,现摘要报告如下:     

人淋巴细胞ANAE反应的阳性率和反应模型的观察及其临床应用的探讨

本文用酸性α-醋酸萘酯酶(ANAE)反应标记淋巴细胞的细胞化学方法,对80例正常人外周血淋巴细胞的ANAE阳性率进行了观察,见到正常人外周血淋巴细胞的ANAE阳性率为74.1±7.3％,其中ANAE反应为“点状颗粒型”的淋巴细胞百分率为61.5±7.3％,“分散颗粒型”的淋巴细胞百分率为12.6±3.5％。同时我们对20例癌症患者(胃癌10例,乳腺癌5例,直肠癌5例)进行了观察,发现三组癌症患者外周血的淋巴细胞ANAE阳性率都显著地低于正常人(P<0.01),“点状颗粒型”淋巴细胞的百分率也显著地低于正常人(P<0.01),但是,“分散颗粒型”淋巴细胞的百分率和正常人比较没有差别。     

癌症患者心理状况及个性分析

为了解癌症患者心理状况及个性特征,采用SCL—90、EPQ测查120例,并以120例正常人作对照。结果显示．癌症病人躯体化、强迫症状、抑郁、焦虑、恐怖、精神病性等6个因子分,较正常人显著增高,EPQ神经质分较对照组显著增高。     

脑囊虫病患者外周血单个核细胞体外诱生IL-2、IFN-γ及TNF-α活性的研究

本文采用生物活性测定法检测了３１例脑囊虫病患者外周血单个核细胞（ＰＢＭＣ）体外诱生ＩＬ－２、ＩＦＮ－γ及ＴＮＦ－α活性，以３０例正常人作对照，探讨了脑囊虫病人细胞因子的变化及在免疫调节中的作用。结果显示：脑囊虫病患者ＰＢＭＣ体外诱生的ＩＬ－２、ＩＦＮ－γ水平明显降低，与正常人相比差异显著（Ｐ＜０．０５，Ｐ＜０．０１），而患者ＰＢＭＣ体外诱生的ＴＮＦ－α水平明显高于正常对照组（Ｐ＜０．０１）；提示脑囊虫病患者的ＣＤ＋４Ｔｈ１细胞呈低应答状态，脑囊虫病患者过量产生的ＴＮＦ－α可能是造成脑囊虫病的免疫病理损害原因之一。     

微量元素、HP及COX-2与胃癌的相关性研究

为了研究微量元素、HP与胃癌发生的相关性 ,取甘肃河西地区 5 0例胃癌患者作为研究组 (R组 ) ,取当地 5 0例健康志愿者作为对照组 (C组 ) ,监测血清微量元素、组织中幽门螺旋杆菌 (HP)以及环氧合酶 - 2 (COX- 2 )的表达。结果显示胃癌组血清微量元素 Cu/ Zn、Fe值高于对照组 (P<0 .0 1,0 .0 5 ) :Zn、Mn低于对照组 (P<0 .0 1,0 .0 5 ) ,经多因素非条件 L ogistic回归分析 ,进入方程的有 Zn(P<0 .0 1)。胃癌组 HP感染率和 COX- 2表达阳性率分别为 88%和 78% ,而对照组则分别为 4 2 %和 0 (P<0 .0 5 ) ,提示 Zn降低可能为胃癌发生的癌前因素 ,其可诱发 HP的感染和 COX- 2的高表达而发生胃癌 ,测定血清中微量元素可提高胃癌的诊断率 ,调整体内微量元素结构可达到对胃癌的化学干预     

Serum and tissue trace elements in Iranian camels (Camelus dromedarius)

Twenty-six male and female (nonpregnant and nonlactating) apparently healthy adult (5 to 10 years) field camels (Camelus dromedarius) were studied to provide data regarding the normal values of trace elements in serum and different tissues. Blood samples were collected by jugular venepuncture and serum was separated by centrifugation. Tissue samples (liver, heart, striated muscle, spleen, kidney, and hair) were collected during postmortem examinations. All the samples were digested and analyzed for copper, iron, cobalt, and molybdenum using atomic absorption spectrophotometry. The results showed that the highest concentration of iron was present in the spleen and that the concentrations of this element in the liver and kidney were higher than those of the heart, striated muscle, serum, and hair (p<0.05). The lowest mean iron concentration was observed in the serum (p<0.05). The mean copper concentration was highest in the liver in comparison to other tissues (p<0.05). No significant differences in cobalt concentrations were detected among different compartments. The mean molybdenum concentration of the striated muscle, heart, kidney, spleen, and liver were significantly higher than those of the serum and hair (p<0.05). No difference due to sex was detected in different tissue and serum concentrations of trace elements.     

Serum and tissue trace elements in Iranian camels (Camelus dromedarius)

There were 26 male and female (nonpregnant and nonlactating) apparently healthy adult (5 to 10 years) field camels (Camelus dromedarius) studied to provide data regarding the normal values of trace elements in serum and different tissues. Blood samples were collected by jugular venepuncture and serum was separated by centrifugation. Tissue samples (liver, heart, striated muscle, spleen, kidney, and hair) were collected during postmortem examinations. All the samples were digested and analyzed for copper, iron, cobalt, and molybdenum using atomic absorption spectrophotometry. The results showed that the highest concentration of iron was present in the spleen and that the concentrations of this element in the liver and kidney were higher than those in the heart, striated muscle, serum, and hair (p<0.05). The lowest mean iron concentration was observed in the serum (p<0.05). The mean copper concentration was highest in liver in comparison to other tissues (p<0.05). No significant differences in cobalt concentrations were detected among different compartments. The mean molybdenum concentration of striated muscle, heart, kidney, spleen, and liver were significantly higher than those of serum and hair (p<0.05). No difference due to sex was detected in different tissue and serum concentrations of trace elements.     

Trace elemental distribution in the scalp hair of bipolars using PIXE technique

Trace metals play a significant role in neurological disorders. There is very limited information available on the role of macro and trace elements in bipolar disorders. The objective of this investigation was to identification, quantification of essential trace elements in the scalp hair samples of the patients and compare with those of normal subjects. We made a hypothesis about the role played by essential trace metals whose concentrations are significantly different to those of normals in the disease process. The analysis was carried out in the scalp hair samples of 26 male and 26 female patients suffering from bipolar disorder (BD) by Particle Induced X-ray Emission Technique (PIXE). The concentration of Cu (p < 0.002) was found to be higher in the hair samples of male bipolar disorder patients while the concentrations of Mn (p < 0.001), Fe (p < 0.005), Zn (p < 0.0001) and Se (p < 0.005) were found to be lower than those in normal subjects. The concentration of Cu (p < 0.0001) was higher in the hair samples of female bipolar patients but depressed levels of Fe (p < 0.005), Ni (p < 0.05), Zn (p < 0.00001) and Se (p < 0.05) were observed compared to controls. Cu/Zn ratio was found to be higher in the hair samples of male and female patients compared with normals. While the imbalance of certain trace elements leads to generation of more free radicals, the imbalance of some other trace elements causes changes in dopamine (neurotransmitter) activity. It is essential to monitor before and periodically during treatment the levels of essential trace elements for effective treatment of bipolar disorder.     

Age-related differences in hair trace elements: a cross-sectional study in Orenburg,Russia

Abstract

Background: Age-related differences in the trace element content of hair have been reported. However, some discrepancies in the data exist.

Aim: The primary objective of this study was to estimate the change in hair trace elements content in relation to age.

Subjects and methods: Six hundred and eighteen women and 438 men aged from 10–59 years took part in the current cross-sectional study.

Results and conclusions: Hair Cr, Mn, Ni, Si, Al, As, Be, Cd and Pb tended to decrease with age in the female sample, whereas hair Cu, Fe, I, Se, Li and Sn were characterised by an age-associated increase. Hair levels of Cr, Cu, I, Mn, Ni, Si and Al in men decreased with age, whereas hair Co, Fe, Se, Cd, Li and Pb content tended to increase. Hair mercury increased in association with age in men and in women, whereas hair vanadium was characterised by a significant decrease in both sexes. The difference in hair trace element content between men and women decreased with age. These data suggest that age-related differences in trace element status may have a direct implication in the ageing process.     

Indicators of Trace-Element Status of Children Living in Rural Areas

The content of essential and toxic elements, except beryllium and mercury, in the hair of examined children (girls aged 7–9 years) is within the biologically acceptable levels set by WHO. The data on the content of essential trace elements suggest that urgent measures aimed at normalization of the elemental status of children are required.     

Contents of bioelements and toxic metals in a Polish population determined by hair analysis. Part 2. Young persons aged 10-20 years.

The aim of this study was to define referential values of 5 basic bioelements (Ca, Mg, Zn, Cu, Fe) in the hair of Polish young persons aged 10-20 years, based on the research conducted from 1991 to 2004 on a group of over 3420 healthy young persons. Mean concentrations of two toxic metals (Pb and Cd) in over 2000 young men were also investigated. The results of biochemical tests were analyzed with the program STATISTICA 7.0 (StatSoft PL). Significant differences between girls' and boys' hair Ca, Mg and Zn in the same age group were observed (p = 0.0000). It was determined that boys have smaller concentrations of hair Ca, Mg and Zn than girls. No significant differences were observed between concentrations of Cu and Fe in the hair of girls and boys of the same age in the tested period. Significantly greater amounts of Pb and Cd characterize boys. Analyses of correlations confirm significance (p < 0.05000) of synergistic interactions between bioelements: Ca-Mg (r = +0.80), Ca-Zn (r = +0.52), Ca-Cu (r = +0.14), Mg-Zn (r = +0.50), Mg-Cu (r = +0.13), Zn-Cu (r = +0.11), Cu-Fe (r = +0.04). Significance (p < 0.05000) of antagonistic interactions of bioelements with toxic metals: Zn-Pb (r = -0.30), Mg-Pb (r = -0.20), Ca-Pb (r = -0.18) and Zn-Cd (r = -0.07) was confirmed. Significance (p < 0.05000) of synergistic effects with toxic elements: Pb-Cd (r = +0.34), Fe-Pb (r = +0.22), Fe-Cd (r = +0.13) was determined. Compatibility of the statistically mapped basic characteristics of young persons' developmental age with the functions of concentrations in tested hair bioelements was confirmed. Young persons whose concentrations of selected hair bioelements showed values outside the reference ranges and who had higher concentrations of toxic metals should undergo further diagnostic tests since the results of previous tests could be a sign of disturbances leading to various diseases. Analyses of the concentrations of bioelements and toxic metals in humans based on hair analysis can be useful as a convenient, non-invasive and painless method in the diagnosis of pathological states. Combined with other analytical data, this method can be used by practising physicians as a complementary diagnostic procedure.     

Role of micro-nutrients and trace elements in carcinoma of larynx

Cancer of the larynx is fourteenth most common cancer in the world. Evidence from case-control studies conducted in developed as well as developing countries suggest that specific micro-nutrients and trace elements have been implicated to be significantly associated with laryngeal carcinoma. The association of these micro-nutrients and trace elements with laryngeal cancer has been discussed in this review article.     

Line-focusing x-ray monochromator for the analysis of trace elements in biological specimens.

A line-focusing x-ray monochromator has been designed and developed to be used with an energy dispersive detection system for the quantitative analysis of trace elements in biological specimens. This instrument uses a cylindrically curved Johansson-type crystal to monochromatize and focus the excitation radiation into a line 0.5 mm wide and 40 mm long. The excitation radiation chosen for these experiments was Mo Kalpha and the total intensity of the line-focused beam was estimated to be 2 X 10(7) cps. Because of the narrow width and high power density of the excitation between beam, small tissue specimens 1 X 1 X 12 mm in size along with a single strand of hair were analyzed for trace elements from Al through Sr. For the transition elements, concentrations of a few parts per million were easily detected with this analyzer.     

浅析未成年人失范的心理压力及应对策略

目前,我国18岁以下的未成年人约有3．67亿。未成年人的思想道德、心理行为将直接关系到中华民族的整体素质,关系到国家的前途和命运,关系到我们中国在世界民族之林中的威望,关系到社会主义现代化事业是否后继有人的问题。且时下一些未成年人思想道德素质低下、违法乱纪行为呈上升趋势,这不仅是中国而且是世界性问题。因此,为了把未成年人培养成为祖国未来的建设者和社会主义事业的接班人,使他们在新形势下能健康成长和全面发展,我们特对未成年人的心理行为现状进行了全面分析,探讨了导致未成年人产生失范行为的“不良的早期社会化、不良的心理需要、畸形的心理动机、错误的认知和错误的人生观价值观”等心理原因和社会原因。着重提出了加强对未成年人心理健康教育和思想道德建设,强化对未成年人学校、家庭和社会管理机制,积极营造有利于未成年人健康成长的社会文化氛围,以及建立危机干预机构,形成社会支持系统,对处于危机中的未成年人提供帮助,对突发事件给予及时干预等应对策略。以全面提高未成年人的素质,增强辨别和抵制能力,防止、减少和遏制其失范行为的发生,使未成年人以健康的心态和全新的风貌展现在新世纪。