过敏性紫癜皮损免疫球蛋白和补体的检测

目的 探讨免疫球蛋白和补体在过敏性紫癜发病中的作用.方法 应用免疫组化LAB-SA法对过敏性紫癜皮损及健康皮肤进行IgG、IgA、IgM及补体C3检测.结果 过敏性紫癜皮损IgG、IgA、IgM及补体C3阳性检出率分别为90.91%、63.64%、90.91%、27.27%,IgG与补体C3、IgM与补体C3差异有统计学意义(P＜0.05).8例健康皮肤均呈阴性.结论 免疫复合物在过敏性紫癜发病中可能起重要作用.     

组胺诱导试验在结节性红斑及结节性血管炎中的意义

本采用组胺诱导试验后DIF法及常规DIF法分别对29例（17例EN和12NV）和31例（16例EN和15例NV）急性期皮肤血管炎患进行了检测组织中沉积免疫复合物的对照研究，发现组胺诱导试验不仅可提高DIF的阳性率有助于诊断，而且经组胺诱导后血管壁IC荧光强度与病情密切相关因而还可作为临床评价病情和疗效判断的指标。     

下肢红斑结节性皮损与结核关系的探讨

目的 了解下肢红斑结节性皮损与结核的关系。方法 对50例下肢红斑结节性皮损的患者进行结核菌素及X线胸片检查。结核菌素试验强阳性者进行抗结核治疗。结果 结核菌素试验强阳性的有42例，占总数的84.0%。其中10例有明确肺结核病史。经抗结核治疗后41病例1月内明显好转，2月内原发皮损消退而痊愈。无肺结核的患者疗程为3个月，有肺结核者疗程为6个月。所有病例治疗完成后，半年内未见复发。结论 结核杆菌感染可能是下肢红斑结节性皮损最常见的原因。     

结节性红斑与硬结性红斑皮损原位抗原研究

采用ＰＡＰ法对结节性红斑、慢性游走性结节性红斑、硬红斑及结节性血管炎４种疾病共３５例的原位抗原进行检测。结果在血管的检出例数分别为１１／１２，８／８，８／８，６／７；在胶原纤维表面的检出例数分别为１１／１２，７／８，８／８，６／７；所有病例表皮角朊细胞内均能检出；在血管腔内与血管腔外的红细胞表面的检出例数除慢性游走性结节性红斑为５／８外，其余病种均能检出。提示结节性红斑等４种疾病均为存在免疫复合物     

结节性红斑的诊疗进展

结节性红斑是以皮下脂肪炎症反应为特征的炎症性疾病,临床表现为双下肢疼痛性红斑。病因主要包括特发性、感染、自身免疫性疾病、妊娠、药物、肿瘤等。相关检查包括皮损活检、血沉、抗“O”、结核试验、胸片、血常规等。应该对症对因治疗。如非甾体抗炎药、糖皮质激素、碘化钾、沙利度胺等免疫抑制剂可以用于严重难治性病例。中医中药治疗及物理治疗也有较好疗效。     

原位聚合酶链反应技术检测结节性红斑皮损中Ⅱ型单纯疱疹...

目的 探讨结节性红斑（ＥＮ）的发病与Ⅱ型单纯疱疹病毒（ＨＳＶ－２）的关系。方法 用原位聚合酶链反应法（ＩＳＰＣＲ）,对３５ＮＥ皮损组织及１４例正常皮肤组织切片中的ＨＳＶ－２ＤＮＡ进行扩增,ＳＰ免疫组化染色,ＡＥＣ显色,结果 ＨＳＶ－２ＤＮＡ在ＥＮ及正常皮肤中的检出率分别为５７．１％（２０／３５）、８．３％（２／１４）,Ｐ〈０．０１,阳性信号主要位于皮损部位,以小血管为著,细胞内的分布以核内型为主,     

结节性红斑患者血浆血小板颗粒膜蛋白及VonWillebrand因子检测

皮肤血管炎发病机理多认为是ＩＣ沉积于血管壁 ,激活补体 ,引起生物因子活化致血管损伤和炎症。ＩＣ、补体活化、血小板活化及内皮细胞功能等相互影响、相辅相成。本文对３２例结节性红斑（ＥＮ）患者血浆血小板颗粒膜蛋白（ＧＭＰ １４０）、ＶｏｎＷｉｌｌｅｂｒａｎｄ因子（     

慢性结节性红斑1例

患者女,36岁.因双小腿结节、斑块及触痛4个月,于2010年2月28日到我科就诊.患者4个月前无明显诱因先后于两小腿出现板栗大红色结节,疼痛不明显,触之稍痛,未引起重视.     

结核相关结节性红斑的研究进展

结节性红斑(erythema nodosum,EN)是一种常见的间隔性脂膜炎,以红斑、炎症性皮下结节为主要表现。其病因复杂,目前认为部分EN与结核菌感染关系密切。结核相关EN好发于女性及青少年,最常见的结核病类型是原发型结核和淋巴结结核,但大多数患者缺乏相应结核病表现。其发病机制认为与免疫复合物介导的以及细胞介导的免疫机制有关。EN主要根据皮损病理诊断确诊,其组织学表现为不伴血管炎的间隔性脂膜炎,多有间隔增厚,伴各种炎性细胞浸润。当怀疑结核菌感染时,则需完善结核菌素试验、结核抗体、胸片、痰培养等相关检查以明确诊断。认为即使缺乏明确的结核感染证据,对于结核菌素皮肤试验强阳性的患者,尤其是在结核流行病区,应该进行抗痨治疗。     

结节性红斑女性因素的临床分析

结节性红斑女性因素的临床分析张堂德，曹化琴，邱贤文，梁应权结节性红斑好发于女性，女性在不同年龄有不同的生理性变化。目前尚没有报道充分说明这些生理变化是否与结节性红斑的产生与发展有关。本文从临床上探讨４１例女性结节性红斑与多种女性因素之间的关系。１．临...     

Tissue and blood T-lymphocyte subpopulations in erythema nodosum leprosum

To study T lymphocytes in erythema nodosum leprosum (ENL), monoclonal antibodies were used to identify T-lymphocyte subpopulations in the blood and skin lesions of patients with ENL and patients with nonreactional lepromatous leprosy. The blood of nonreactional lepromatous patients had a lymphopenia and a proportionate reduction in pan T cells, helper-inducer, and suppressor-cytotoxic subsets, but a normal helper-suppressor ratio, as compared with controls. Patients with ENL did not differ significantly from the controls. In skin lesions, an admixture of helper and suppressor phenotypes among foamy histiocytes was found. The ENL tissue had more numerous cells of the helper-inducer phenotype and fewer of the suppressor-cytotoxic phenotype, as compared with nonreaction lepromatous tissues. In 22 patients with simultaneous examination of tissue and blood T-cell subsets, there was no correlation between tissue and blood helper-suppressor ratios, indicating that some sort of selection process brings lymphocytes into tissues from peripheral blood.     

结节性红斑患者血清总IgE和MAO水平测定

目的：检测结节性红斑患者血清总IgE和单胺氧化酶（MAO）含量。方法：选择结节性红斑患者115例及正常人36名,应用化学发光免疫分析仪和全自动生化分析仪分别检测其血清总IgE和MAO。结果：结节性红斑患者血清总IgE含量235．36±295．14IU／mL高于健康对照组83．19±82．52IU／mL（P〈0．05）,血清MAO含量15．55±14．32U／L高于健康对照组7．43±3．76U／L（P〈0．01）。结论：IgE和MAO可能参与了结节性红斑的发病过程。     

Detection of spirochaetal DNA simultaneously in skin biopsies, peripheral blood and urine from patients with erythema migrans

Lyme borreliosis is an emerging zoonosis transmitted by infected hard-bodied ticks. The disease is multisystemic. In the initial stage its typical manifestation is the erythema migrans, a cutaneous lesion that occurs in up to 90% of patients. In order to investigate the presence of the specific agent, Borrelia burgdorferi, in the early stages of the disease, DNA from skin biopsies, urine and peripheral blood of 30 patients with clinically documented erythema migrans and without apparent systemic involvement was analysed by polymerase chain reaction. Borrelia DNA in both blood and skin biopsies was detected in 23 patients, while in 9 patients it was discovered in urine and skin biopsies. These results demonstrate that Borrelia DNA is detectable systemically also in patients with early Lyme borreliosis and strongly suggest a possible dissemination of the causative agents even when only a local infection is assumed.     

硬红斑皮损中结核杆菌DNA的检测

为了探讨皮肤血管炎和结核杆菌的关系。应用PCR技术对硬红斑病理组织进行了结核杆菌DNA的检测,结果显示4例作为阳性对照的淋巴结结核中3例呈阳性,正常皮肤组织及5例硬红斑均呈阴性,表明完整的结核杆菌在硬红斑并不多见,可能部分由其产生的蛋白或细菌碎片引起的变态反应所致。     

Histopathology of erythema nodosum

The histopathology of erythema nodosum is discussed with reference to the literature and of 19 cases of our clinic. There is a significant vascular involvement especially of the subcutaneous tissue. The veins and arteries are equally affected. The histological picture consists not only of an acute inflammatory process in the subcutaneous tissue, but also of inflammatory cells which area see to a lesser extent in the dermis. In many cases the typical small granulomatous foci of MIESCHER which are considered to be pathognomonic for erythema nodosum do not develop. In the cases which we examined we were not always able to tell from the histological picture how long the erythema nodosum had been present. Multinucleated giant cells which are often found in the septal connective tissue are seen even in relatively acute cases.