急性脊髓损伤患者外周血淋巴细胞CD69的检测及其临床意义

目的：探讨急性脊髓损伤（acute spinal cord injury,ASCI)患者外周血CD69的表达及其临床意义。方法：应用免疫荧光标记流式细胞仪检测15例急性SCI患者外周血淋巴细胞CD69的表达，并与正常人群进行比较。结果；急性SCI患者CD69值显著高于正常值（P＜0．01）。结论：外周血淋巴细胞对急性SCI的刺激产生了积极的免疫应答，相当一部分外周血淋马细胞活化，可能这种早期的非特异性应答严重严重损害了机体免疫能力（一种消耗性损伤），而使感染率明显上升，另外也提示CD69可能用于与SCI预后的判断。     

监测肾移植受者外周血淋巴细胞选凝素-L表达的临床意义

目的　探讨肾移植前后外周血淋巴细胞选凝素 L的表达水平变化与肾移植排斥反应的关系。　方法　应用单克隆抗体 流式细胞仪荧光抗体技术监测 33例肾移植受者外周血淋巴细胞选凝素 L的表达水平。　结果　肾移植术后所有患者选凝素 L表达水平较术前显著降低 ,排斥反应时排斥组明显升高 (49.1± 13.8) ,同期稳定组无明显变化 (36 .5± 12 7)。　结论　选凝素 L与肾移植排斥反应密切相关 ,抑制选凝素 L表达是联合应用免疫抑制剂防治排斥反应的重要作用机制 ,监测选凝素 L的表达水平有助于肾移植排斥反应的诊断。     

抗Tac单抗诱导治疗对肾移植受者外周血淋巴细胞CD分子表达的影响

目的:探讨首次尸肾移植受者使用抗Tac单抗诱导治疗后外周血淋巴细胞CD分子的动态变化及其临床意义。方法:对首次尸肾移植的患者,在三联免疫抑制方案(激素+骁悉+新山的明)基础上,给予两剂抗Tac单抗诱导治疗,其中舒莱组30例,赛呢哌组28例。以流式细胞仪检测术前,术后第1天、1周、2周、4周、6周及8周外周血淋巴细胞CD分子的表达。结果:移植受者外周血淋巴细胞CD分子变化明显,其中CD25在术后明显下降(P<0.05),并维持4~6周;CD40亦明显下降(P<0.05),术后2周时开始回升;CD86、CD28、CD80、CD95等均有下降,但各组间差异无统计学意义。舒莱组和赛呢哌组CD分子表达差异亦无统计学意义。结论:抗Tac单抗可以有效地封闭外周血活化淋巴细胞表面的CD25,抑制其增殖和分化;通过某种机制,抗Tac单抗可以降低外周血淋巴细胞CD40的表达,可能起到抑制B淋巴细胞活化的作用。     

前列腺癌患者外周血淋巴细胞CD44v6和CD54的表达及临床意义

目的 探讨前列腺癌患者外周血淋巴细胞CD44V6、CD54的表达及临床意义.方法 采用流式细胞术对前列腺癌及前列腺增生患者正常成人外周血淋巴细胞CD44V6、CD54的表达水平进行检测.结果 前列腺癌患者外周血CD44V6、CD54的表达水平(296.47±44.51,187.41±28.07)显著高于良性病变组(82.41±13.87,42.70±8.9)及正常对照组(76.13±11.04,37.25±5.35,P＜0.01).其表达水平与肿瘤病理类型和临床分期成正相关.结论 前列腺癌患者外周血淋巴细胞CD44V6、CD54的高表达与肿瘤分期及转移密切相关,检测其表达的动态变化可以为评估肿瘤发展及疗效判断提供依据.     

肾移植患者CD38和CD95在外周血T细胞亚群中表达水平的观察

肾移植是目前开展的最多的同种异体器官移植手术,但排斥反应和病毒感染等诸多因素还严重影响着患者和移植物的存活期。我们运用流式细胞术对50例肾移植患者进行外周血T细胞表面CD38和CD95的动态监测,探讨其与排斥反应关系,现报告如下。对象和方法1.研究对象:正常对照60例,男女各半,年龄27～64岁;肾移植患者组50例,其中男性34例,女性16例,年龄29～57岁。     

基因扫描分析肾移植前后外周血T淋巴细胞谱系的变化

目的 了解肾移植患者移植前后外周血单核细胞T淋巴细胞受体(TCR)Vβ基因谱系的变化和是否存在T淋巴细胞的特征性克隆增殖。方法 通过逆转录聚合酶链反应(TR-PCR)扩增11例首次肾移植患者术前及术后外周血单核细胞TCR Vβ24个亚家族基因，了解其表达情况；阳性的PCR产物进一步经荧光素标记和基因扫描的方法分析TCR的互补决定区3(CDR3)的长度及序列。结果 11例患者术前分别表达1～10个TCR Vβ亚家族，大部分为多克隆；术后第20d分别表达1～5个亚家族，绝大部分为寡克隆；大部分TCR Vβ亚家族与术前相同。发生急性排斥反应的1例在排斥反应冲击治疗前无TCR Vβ亚家族表达，冲击治疗过程中表达5个Vβ亚家族，排斥逆转后表达2个Vβ亚家族(多克隆)；1例肾功能恢复延迟者在恢复中表达4个Vβ亚家族，恢复后表达5个Vβ亚家族，以多克隆为主。结论 肾移植患者术前外周血T淋巴细胞存在着TCR Vβ亚家族倾斜分布的现象，术后20d倾斜分布更明显；急性排斥反应与某些特异性T淋巴细胞克隆有关，随着排斥反应的逆转，这些克隆随之消失；排斥反应逆转和肾功能恢复正常后的多克隆表达可能提示免疫状态趋于正常化。     

颈髓损伤外周血淋巴细胞的表达及临床意义

目的 探讨颈髓损伤（cervical cord injuries,CCI)外周血淋巴细胞表达的相互作用及临床意义。方法 用免疫荧光标记流式细胞仪检测了20例急性颈髓损伤患者外周血淋巴细胞CD19、CD3、CD4、CD8、CD69、CD54、CD56表达的变化,并与正常人群进行比较。结果 T细胞CD3、CD4和CD4／CD8比值显著降低（P＜0．01）。而早期活化标志CD69则显著升高（P＜0．01）。B细胞CD19表达明显增加,粘附分子CD56和CD54表达也明显增加（P＜0．01）。结论 以上结果提示急性颈髓损伤后机体细胞免疫功能发生明显变化,主要表面为T细胞的减少,B细胞的增加以及淋巴细胞活化抗原和粘附分子的表达增加。CCI对细胞功能造成严重损伤,机体免疫系统对CCI的刺激则表面为产生能力的增强和活化及粘附功能的反应性增强。     

CD15抗原在胃癌组织中的表达

目的 探讨CD15抗原在胃癌组织中的表达及分布特点,及其与肿瘤发生发展、转移的关系。方法 应用微波－LSAB免疫组化法,对95例胃癌组织及癌旁粘膜组织进行CD15抗原检测,并进一步用胶体镜技术对CD15抗原的分布特征进行观察。结果 95例胃癌中,82例呈CD15阳性表达,阳性率86．3％;癌旁粘膜亦呈CD15弱阳性表达。CD15表达阳性率在伴有淋巴结转移的胃癌中为92．6％,显著高于无转移者的70．4％（P＜0．005）。免疫电镜显示,CD15抗原主要分布于胃癌细胞质的界膜、内质网、高尔基体及近细胞核膜处和癌旁粘膜上皮细胞质的胃膜处。结论 胃癌的发生发展及侵袭、转移与CD15的表达相关,CD15可能是通过改变其糖基构型而参与肿瘤的形成或转移过程。     

肾移植受者外周血白细胞巴细胞功能相关抗原—1表达的意义

目的 监测肾移植前、后外周血白细胞淋巴细胞功能相关抗原－１（ＬＦＡ－１）的表达水平,了解其变化规律,探讨其在肾移植排斥反应中的意义。方法 应用单克隆抗体－流式细胞仪荧光抗体技术监测肾移植受者得外周血白细胞的ＬＦＡ－１表达水平。结果 肾移植术后ＬＦＡ－１表达水平较术前显著降低,并维持在低水平,发生排斥反应时间显著升高;排斥时的ＬＦＡ－１表达水平与术后无排斥时及排斥反应控制后比较,差异有显著性,与术前     

测定CD3 8+ CD8+ T淋巴细胞在监测肾移植后巨细胞病毒感染中的作用

目的观察CD38+CD8+T淋巴细胞水平在肾移植后巨细胞病毒感染患者体内的变化,探讨其监测肾移植后巨细胞病毒活动性感染的可能性.方法分别应用流式细胞术和免疫组织化学方法测定56例肾移植受者手术前后的CD38+CD8+T淋巴细胞水平和巨细胞病毒白细胞抗原,并将两者结果进行比较.结果肾移植术前所有患者巨细胞病毒白细胞抗原均为阴性,其(CD38+CD8+)/CD8+的平均比值为0.11±0.05;肾移植后检测到有14例患者巨细胞病毒白细胞抗原阳性,出现阳性的时间为术后(32.7±16.6)d,(CD38+CD8+)/CD8+的比值在术后(29.6±8.4)d出现了有显著意义的升高,平均数值为0.43±0.21.这些患者接受静脉滴注丙氧鸟苷治疗后巨细胞病毒白细胞抗原转阴,(CD38+CD8+)/CD8+平均比值下降为0.16±0.09.治疗前后CD38+CD8+T淋巴细胞水平比较差异有显著性意义(P＜0.05).结论CD38+CD8+T淋巴细胞水平的检测结合巨细胞病毒白细胞抗原检查有助临床监测肾移植后CMV活动性感染.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

目的 探讨肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义.方法 采用流式细胞术检测2007年2月至2008年6月期间,肾移植术后早期发生严重肺部感染的28例患者(感染组)外周血CD4+T淋巴细胞计数的变化,并随机选取同期肾移植术后病情稳定的30例患者(对照组)作为对照.结果 肾移植术后早期,感染组患者入院第1天CD4+T淋巴细胞计数显著低于对照组,分别为(184.1±117.5)个/μl和(518.6±232.7)个/μl(P<0.01).感染组患者中有5例治疗无效死亡,其中4例CD4+T淋巴细胞计数呈持续降低趋势;感染组中存活的患者在治疗恢复后,CD4+T淋巴细胞计数明显上升至(406.5±163.9)个/μl,与治疗前比较,P<0.01.受试者工作特征(ROC)曲线分析表明,CD4+T淋巴细胞计数减少能作为判断发生肺部感染的有效指标,其曲线下面积(AUC)为94.9%(P<0.01),CD4+T淋巴细胞计数为220个/μL时,其特异度为100%.结论 外周血CD4+T淋巴细胞的变化与肾移植术后早期严重肺部感染的转归密切相关.CD4+T淋巴细胞计数低于220个/μl的患者发生感染的可能性极大;测定外周血CD4+T淋巴细胞计数并动态分析对于优化治疗和判断预后有重要的参考价值.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

Objective To explore the clinical implication of peripheral blood CD4+ T-cell counts in renal allograft recipients with severe pulmonary infection in the early stage after kidney transplantation. Methods From February 2007 to June 2008, we investigated the variation of peripheral blood CD4+ T-cell counts using flow cytometry in 28 cases of severe pulmonary infection 1 ～6 months after kidney transplantation (infection group), and 30 cases (control group) randomly selected that had stable situation and normal kidney function in the same period. Results CD4+ T-cell counts on the day of admission in infection group were significantly lower than in control group (184.1 ±117.5/μl vs. 518.6±232.7/μl, P<0.01 ). In infection group, 5 patients died and 4 of them had obviously declining trends of CD4+ T-cell counts during hospitalization course. Comparing to the day of admission, CD4+ T-cell counts of those survivors in infection group were significantly increased (184.1±117.5/μl vs. 406.5±163.9/μl, P<0.01) when infections were controlled. ROC analysis showed that CD4+ T-cell counts on the day of admission were accurate enough to identify who were susceptible to infection. In detail, the area under the curve (AUC) was 94.9% (P<0.01). CD4+ T-cell counts of 220/μl displayed the minimal misdiagnosis rate. Conclusions The variations of CD4+ T-cell counts are correlated to onset and progression of severe pulmonary infection in the early stage after kidney transplantation. Those who had CD4+ T-cell counts lower than 220/μl were at high risk of pulmonary infection. Direct measure and dynamic analysis of CD4+ T-cell subset have an important role in optimizing treatment and predicting prognosis of severe pulmonary infection in the early stage after kidney transplantation.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

Objective To explore the clinical implication of peripheral blood CD4+ T-cell counts in renal allograft recipients with severe pulmonary infection in the early stage after kidney transplantation. Methods From February 2007 to June 2008, we investigated the variation of peripheral blood CD4+ T-cell counts using flow cytometry in 28 cases of severe pulmonary infection 1 ～6 months after kidney transplantation (infection group), and 30 cases (control group) randomly selected that had stable situation and normal kidney function in the same period. Results CD4+ T-cell counts on the day of admission in infection group were significantly lower than in control group (184.1 ±117.5/μl vs. 518.6±232.7/μl, P<0.01 ). In infection group, 5 patients died and 4 of them had obviously declining trends of CD4+ T-cell counts during hospitalization course. Comparing to the day of admission, CD4+ T-cell counts of those survivors in infection group were significantly increased (184.1±117.5/μl vs. 406.5±163.9/μl, P<0.01) when infections were controlled. ROC analysis showed that CD4+ T-cell counts on the day of admission were accurate enough to identify who were susceptible to infection. In detail, the area under the curve (AUC) was 94.9% (P<0.01). CD4+ T-cell counts of 220/μl displayed the minimal misdiagnosis rate. Conclusions The variations of CD4+ T-cell counts are correlated to onset and progression of severe pulmonary infection in the early stage after kidney transplantation. Those who had CD4+ T-cell counts lower than 220/μl were at high risk of pulmonary infection. Direct measure and dynamic analysis of CD4+ T-cell subset have an important role in optimizing treatment and predicting prognosis of severe pulmonary infection in the early stage after kidney transplantation.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

Objective To explore the clinical implication of peripheral blood CD4+ T-cell counts in renal allograft recipients with severe pulmonary infection in the early stage after kidney transplantation. Methods From February 2007 to June 2008, we investigated the variation of peripheral blood CD4+ T-cell counts using flow cytometry in 28 cases of severe pulmonary infection 1 ～6 months after kidney transplantation (infection group), and 30 cases (control group) randomly selected that had stable situation and normal kidney function in the same period. Results CD4+ T-cell counts on the day of admission in infection group were significantly lower than in control group (184.1 ±117.5/μl vs. 518.6±232.7/μl, P<0.01 ). In infection group, 5 patients died and 4 of them had obviously declining trends of CD4+ T-cell counts during hospitalization course. Comparing to the day of admission, CD4+ T-cell counts of those survivors in infection group were significantly increased (184.1±117.5/μl vs. 406.5±163.9/μl, P<0.01) when infections were controlled. ROC analysis showed that CD4+ T-cell counts on the day of admission were accurate enough to identify who were susceptible to infection. In detail, the area under the curve (AUC) was 94.9% (P<0.01). CD4+ T-cell counts of 220/μl displayed the minimal misdiagnosis rate. Conclusions The variations of CD4+ T-cell counts are correlated to onset and progression of severe pulmonary infection in the early stage after kidney transplantation. Those who had CD4+ T-cell counts lower than 220/μl were at high risk of pulmonary infection. Direct measure and dynamic analysis of CD4+ T-cell subset have an important role in optimizing treatment and predicting prognosis of severe pulmonary infection in the early stage after kidney transplantation.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

Objective To explore the clinical implication of peripheral blood CD4+ T-cell counts in renal allograft recipients with severe pulmonary infection in the early stage after kidney transplantation. Methods From February 2007 to June 2008, we investigated the variation of peripheral blood CD4+ T-cell counts using flow cytometry in 28 cases of severe pulmonary infection 1 ～6 months after kidney transplantation (infection group), and 30 cases (control group) randomly selected that had stable situation and normal kidney function in the same period. Results CD4+ T-cell counts on the day of admission in infection group were significantly lower than in control group (184.1 ±117.5/μl vs. 518.6±232.7/μl, P<0.01 ). In infection group, 5 patients died and 4 of them had obviously declining trends of CD4+ T-cell counts during hospitalization course. Comparing to the day of admission, CD4+ T-cell counts of those survivors in infection group were significantly increased (184.1±117.5/μl vs. 406.5±163.9/μl, P<0.01) when infections were controlled. ROC analysis showed that CD4+ T-cell counts on the day of admission were accurate enough to identify who were susceptible to infection. In detail, the area under the curve (AUC) was 94.9% (P<0.01). CD4+ T-cell counts of 220/μl displayed the minimal misdiagnosis rate. Conclusions The variations of CD4+ T-cell counts are correlated to onset and progression of severe pulmonary infection in the early stage after kidney transplantation. Those who had CD4+ T-cell counts lower than 220/μl were at high risk of pulmonary infection. Direct measure and dynamic analysis of CD4+ T-cell subset have an important role in optimizing treatment and predicting prognosis of severe pulmonary infection in the early stage after kidney transplantation.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

Objective To explore the clinical implication of peripheral blood CD4+ T-cell counts in renal allograft recipients with severe pulmonary infection in the early stage after kidney transplantation. Methods From February 2007 to June 2008, we investigated the variation of peripheral blood CD4+ T-cell counts using flow cytometry in 28 cases of severe pulmonary infection 1 ～6 months after kidney transplantation (infection group), and 30 cases (control group) randomly selected that had stable situation and normal kidney function in the same period. Results CD4+ T-cell counts on the day of admission in infection group were significantly lower than in control group (184.1 ±117.5/μl vs. 518.6±232.7/μl, P<0.01 ). In infection group, 5 patients died and 4 of them had obviously declining trends of CD4+ T-cell counts during hospitalization course. Comparing to the day of admission, CD4+ T-cell counts of those survivors in infection group were significantly increased (184.1±117.5/μl vs. 406.5±163.9/μl, P<0.01) when infections were controlled. ROC analysis showed that CD4+ T-cell counts on the day of admission were accurate enough to identify who were susceptible to infection. In detail, the area under the curve (AUC) was 94.9% (P<0.01). CD4+ T-cell counts of 220/μl displayed the minimal misdiagnosis rate. Conclusions The variations of CD4+ T-cell counts are correlated to onset and progression of severe pulmonary infection in the early stage after kidney transplantation. Those who had CD4+ T-cell counts lower than 220/μl were at high risk of pulmonary infection. Direct measure and dynamic analysis of CD4+ T-cell subset have an important role in optimizing treatment and predicting prognosis of severe pulmonary infection in the early stage after kidney transplantation.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

Objective To explore the clinical implication of peripheral blood CD4+ T-cell counts in renal allograft recipients with severe pulmonary infection in the early stage after kidney transplantation. Methods From February 2007 to June 2008, we investigated the variation of peripheral blood CD4+ T-cell counts using flow cytometry in 28 cases of severe pulmonary infection 1 ～6 months after kidney transplantation (infection group), and 30 cases (control group) randomly selected that had stable situation and normal kidney function in the same period. Results CD4+ T-cell counts on the day of admission in infection group were significantly lower than in control group (184.1 ±117.5/μl vs. 518.6±232.7/μl, P<0.01 ). In infection group, 5 patients died and 4 of them had obviously declining trends of CD4+ T-cell counts during hospitalization course. Comparing to the day of admission, CD4+ T-cell counts of those survivors in infection group were significantly increased (184.1±117.5/μl vs. 406.5±163.9/μl, P<0.01) when infections were controlled. ROC analysis showed that CD4+ T-cell counts on the day of admission were accurate enough to identify who were susceptible to infection. In detail, the area under the curve (AUC) was 94.9% (P<0.01). CD4+ T-cell counts of 220/μl displayed the minimal misdiagnosis rate. Conclusions The variations of CD4+ T-cell counts are correlated to onset and progression of severe pulmonary infection in the early stage after kidney transplantation. Those who had CD4+ T-cell counts lower than 220/μl were at high risk of pulmonary infection. Direct measure and dynamic analysis of CD4+ T-cell subset have an important role in optimizing treatment and predicting prognosis of severe pulmonary infection in the early stage after kidney transplantation.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

Objective To explore the clinical implication of peripheral blood CD4+ T-cell counts in renal allograft recipients with severe pulmonary infection in the early stage after kidney transplantation. Methods From February 2007 to June 2008, we investigated the variation of peripheral blood CD4+ T-cell counts using flow cytometry in 28 cases of severe pulmonary infection 1 ～6 months after kidney transplantation (infection group), and 30 cases (control group) randomly selected that had stable situation and normal kidney function in the same period. Results CD4+ T-cell counts on the day of admission in infection group were significantly lower than in control group (184.1 ±117.5/μl vs. 518.6±232.7/μl, P<0.01 ). In infection group, 5 patients died and 4 of them had obviously declining trends of CD4+ T-cell counts during hospitalization course. Comparing to the day of admission, CD4+ T-cell counts of those survivors in infection group were significantly increased (184.1±117.5/μl vs. 406.5±163.9/μl, P<0.01) when infections were controlled. ROC analysis showed that CD4+ T-cell counts on the day of admission were accurate enough to identify who were susceptible to infection. In detail, the area under the curve (AUC) was 94.9% (P<0.01). CD4+ T-cell counts of 220/μl displayed the minimal misdiagnosis rate. Conclusions The variations of CD4+ T-cell counts are correlated to onset and progression of severe pulmonary infection in the early stage after kidney transplantation. Those who had CD4+ T-cell counts lower than 220/μl were at high risk of pulmonary infection. Direct measure and dynamic analysis of CD4+ T-cell subset have an important role in optimizing treatment and predicting prognosis of severe pulmonary infection in the early stage after kidney transplantation.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

Objective To explore the clinical implication of peripheral blood CD4+ T-cell counts in renal allograft recipients with severe pulmonary infection in the early stage after kidney transplantation. Methods From February 2007 to June 2008, we investigated the variation of peripheral blood CD4+ T-cell counts using flow cytometry in 28 cases of severe pulmonary infection 1 ～6 months after kidney transplantation (infection group), and 30 cases (control group) randomly selected that had stable situation and normal kidney function in the same period. Results CD4+ T-cell counts on the day of admission in infection group were significantly lower than in control group (184.1 ±117.5/μl vs. 518.6±232.7/μl, P<0.01 ). In infection group, 5 patients died and 4 of them had obviously declining trends of CD4+ T-cell counts during hospitalization course. Comparing to the day of admission, CD4+ T-cell counts of those survivors in infection group were significantly increased (184.1±117.5/μl vs. 406.5±163.9/μl, P<0.01) when infections were controlled. ROC analysis showed that CD4+ T-cell counts on the day of admission were accurate enough to identify who were susceptible to infection. In detail, the area under the curve (AUC) was 94.9% (P<0.01). CD4+ T-cell counts of 220/μl displayed the minimal misdiagnosis rate. Conclusions The variations of CD4+ T-cell counts are correlated to onset and progression of severe pulmonary infection in the early stage after kidney transplantation. Those who had CD4+ T-cell counts lower than 220/μl were at high risk of pulmonary infection. Direct measure and dynamic analysis of CD4+ T-cell subset have an important role in optimizing treatment and predicting prognosis of severe pulmonary infection in the early stage after kidney transplantation.     

肾移植术后早期严重肺部感染患者外周血CD4+T淋巴细胞计数的临床意义

Objective To explore the clinical implication of peripheral blood CD4+ T-cell counts in renal allograft recipients with severe pulmonary infection in the early stage after kidney transplantation. Methods From February 2007 to June 2008, we investigated the variation of peripheral blood CD4+ T-cell counts using flow cytometry in 28 cases of severe pulmonary infection 1 ～6 months after kidney transplantation (infection group), and 30 cases (control group) randomly selected that had stable situation and normal kidney function in the same period. Results CD4+ T-cell counts on the day of admission in infection group were significantly lower than in control group (184.1 ±117.5/μl vs. 518.6±232.7/μl, P<0.01 ). In infection group, 5 patients died and 4 of them had obviously declining trends of CD4+ T-cell counts during hospitalization course. Comparing to the day of admission, CD4+ T-cell counts of those survivors in infection group were significantly increased (184.1±117.5/μl vs. 406.5±163.9/μl, P<0.01) when infections were controlled. ROC analysis showed that CD4+ T-cell counts on the day of admission were accurate enough to identify who were susceptible to infection. In detail, the area under the curve (AUC) was 94.9% (P<0.01). CD4+ T-cell counts of 220/μl displayed the minimal misdiagnosis rate. Conclusions The variations of CD4+ T-cell counts are correlated to onset and progression of severe pulmonary infection in the early stage after kidney transplantation. Those who had CD4+ T-cell counts lower than 220/μl were at high risk of pulmonary infection. Direct measure and dynamic analysis of CD4+ T-cell subset have an important role in optimizing treatment and predicting prognosis of severe pulmonary infection in the early stage after kidney transplantation.