鼠疫菌通过植物毒力变化的研究

用鼠疫菌０６１株接种植物，第１０ｄ分离菌株命名为６１－３，原株小白鼠ＬＤ５０是第１０ｄ分离株的３．５６９倍，第２０ｄ分离菌株命名为６１－２，原株ＬＤ５０是第２０ｄ分离株的３５．１５５倍，６１－３株是６１－２株的９．８５倍，表明随着鼠疫菌在植物组织中时间延长，其毒力增强。     

鼠疫菌通过植物后某些生物学特性的变异

对鼠疫弱毒株０６１及用该株菌接种大豆后不同时期从大豆根中分离的６１－２、６１－３株的某些生物学特性进行比较研究。发现鼠疫菌通过植物后其菌落形态由Ｒ型转变为Ｓ型，但对小白鼠的毒力随在植物体存留时间的延长而增强。未发现原株和植物分离株生化特性变异。     

恶性疟原虫FCC—1／HN株pcDNA3—EBA175／HRPII重组质粒直？…

为观察恶性疟原虫ＦＣＣ－１／ＨＮ株ｐｃＤＮＡ３－ＥＢＡ１７５／ＨＲＰⅡ重组质粒肌肉途径接种后在宿主体内的表达。ＢＡＬＢ／ｃ小鼠分为３组,１组（实验组）经后腿股四头肌注射重组粒ｐｃＤＮＡ３－ＥＢＡ１７５／ＨＲＰⅡ１００μＧ;２组（实验对照组）注射空白质粒ｐｃＤＮＡ３１００μＧ,３组（正常对照组）注射ＰＢＳ（ｐＨ７．４）１００μｌ。每隔２０ｄ加强注射,于第１次接种后２０ｄ和第２次接种后１０ｄ取小鼠双侧     

慢性肝炎患者高压氧治疗前后肝组织中HBsAg、HBcAg检测初步探讨

目的　观察慢性肝炎（ＣＨ） 高压氧（ ＨＢＯ） 治疗前后患者的免疫功能、肝组织中ＨＢｓＡｇ 、ＨＢｃＡｇ 变化。方法：用纯氧单仓治疗（２－５ＭＰａ ,２ｈ／ｄ ,１０ｄ／ｃｙｃ ,６ｃｙｃ）３０ 例ＣＨ,治疗前后用美国ＢＥＣＭＡＮ 公司生产ＡｒｒａｙＴＭＰｒｏｔｅｉｎ Ｓｙｓｔｅｍ ３６０ 测全部患者静脉血白蛋白（ Ａ） 、γ球蛋白（γ） 、ｌｇＡ、ｌｇＧ、ｌｇＭ、和补体Ｃ３ 、Ｃ４ ;二次肝穿刺活检,ＡＢＣ 法检测肝组织中ＨＢｓＡｇ 、ＨＢｃＡｇ 。取算术均数行ｔ 检验。结果：ＨＢＯ 治疗后静脉血γ、ｌｇＧ、ｌｇＭ、明显降低,Ａ、Ｃ３ 明显升高,治疗前后差异显著（ Ｐ＜０－０５） ;肝组织中ＨＢｓＡｇ 、ＨＢｃＡｇ 变化不明显,治疗前后差异不显著（ Ｐ＞ ０－０５） 。结论：ＨＢＯ 治疗ＣＨ,可抑制患者的免疫功能,对ＨＢｓＡｇ 、ＨＢｃＡｇ 抑制作用不明显     

刚地弓形虫细胞培养的研究Ⅳ．临床标本培养检测技术的改进

本文以血清抗弓形虫抗体阳性的孕妇羊水进行细胞培养检测，实验组以ＴＨＰ一１细胞系培养并加入经离心过滤的ＲＨ株速殖子细胞培养上清（ＳＲＣ）作为培养佐剂，在第４～６ｄ，第７～１０ｄ，第１１～１４ｄ进行３次检验；对照组以ＭＲＣ５细胞系培养，在第４ｄ、第８ｄ进行２次常规检测。结果２６例羊水在ＭＲＣ５组全部阴性，而ＴＨＰ一１组分离出３株野生株，并在其后经同时接种小鼠的血清特异性ＩｇＭ抗体和脑包囊检测或脐血特异性ＩｇＭ检测所证实。提示加用ＳＲＣ并延长培养检测时间对提高弓形虫病以细胞培养作病原诊断的敏感性有显著意义。     

应用二氧化碳培养箱连续培养大量恶性疟原虫配子体

目的：为建立在二氧化碳（ＣＯ２）培养箱中连续培养大量恶性疟原虫（Ｐ．ｆ）配子体（Ｇ）的方法。方法：使用加入不同量ＮａＨＣＯ３的培养基，观察比较Ｇ的消长。结果：在培养第５ｄ（ｄ５）出现Ｇ，至ｄ１１－ｄ１３达到高峰；实验组与对照组Ｇ的峰值分别为１．８８％±０．６１％和１．２９％±０．４１％（Ｐ＜０．０５），表明实验组培养后期使用含较高浓度ＮａＨＣＯ３的完全培养基，有助于Ｇ的分化。Ⅰ－Ⅴ期Ｇ的高峰分别出现于培养ｄ５、ｄ７、ｄ１１、ｄ１３和ｄ１５。ｄ１５Ｖ期Ｇ率为７．１％— ５２．６％，平均为２４．３％。雌、雄Ｇ比例为１２．８∶１。原虫从液氮中取出复苏后，加入新鲜红细胞连续分皿２４代，仍保持较高的产生Ｇ的能力。经薄膜饲血器多次人工感染斯氏按蚊（Ａｎｏｐｈｅｌｅｓｓｔｅｐｈｅｎｓｉ），解剖３２８只蚊胃，未见卵囊。结论：本虫株使用该方法可以持续稳定地取得大量Ｐ． ｆＧ。     

人参皂甙通过GATA转录因子刺激巨核系祖细胞增殖

目的：了解人参皂甙（ＧＳ）对巨核系祖细胞（ＣＦＵ－Ｍｅｇ）的刺激增殖作用，比较ＧＳ与生长因子ＴＰＯ、ＩＬ－３对ＧＡＴＡ转录调控蛋白的诱导作用。方法：正常人和ＩＴＰ患者骨髓ＣＦＵ－Ｍｅｇ培养和ＧＳ刺激试验、电泳带移动阻滞试验（ＥＭＳＡ）、抗体胶移动试验（Ｓｕｐｅｒｓｈｉｆｔ ａｓｓａｙ）和蛋白免疫印迹法（Ｗｅｓｔｅｒｎ Ｂｌｏｔ）。结果：ＧＳ刺激正常ＣＦＵ－Ｍｅｇ增殖，呈剂量依赖的方式，血小板减少性紫癜（ＩＴＰ）患者的ＣＦＵ－Ｍｅｇ也对ＧＳ敏感。ＧＳ、ＴＰＯ和ＩＬ－３均能诱导生长因子依赖ＭＯ７ｅ细胞核内ＧＡＴＡ蛋白与ＤＮＡ结合的活性及使ＧＡＴＡ－２蛋白含量增加。ＧＳ＋ＴＰＯ或ＧＳ＋ＩＬ－３可协同地诱导ＧＡＴＡ蛋白活性进一步增高。结论：ＧＳ能够通过类生长因子和增强生长因子作用而刺激ＣＦＵ－Ｍｅｇ增殖，ＧＡＴＡ族转录因子，主要是ＧＡＴＡ－２参与造血细胞对ＧＳ反应的信号传递途径。     

鼠疫菌弱毒株粗制鼠毒素含量及其SDS—PAGE电泳图形比较

鼠疫菌ＥＶｐａｒｉｓ的湿菌体和等湿菌体帛的菌粉，用２．５％ＮａＣｌ浸洗可溶性蛋白质，硫酸 铵分段沉淀法制备粗制鼠毒素，收获量及其ＳＤＳ－ＰＡＧＥ电泳形基本相同。用ＬＢ固体培养基增殖鼠疫菌弱毒株，每株培养面积３００ｃｍ＾２，收获湿功和体ＥＶｐａｒｉｓ株０．６ｇ，ＭⅡ４０株、Ｏｔｔｅｎ株、Ａ１１２２株１．２ｇ，１７５株１．４ｇ。不同菌株每克湿菌 体收获粗制鼠毒素的量分别为：ＭⅡ４０ｓｒｉ９．３４ｍｇ，     

慢性肝炎患者高压氧治疗前后肝组织中HBsAg,HBcAg检测初步 …

目的 观察慢性肝炎高压氧治疗前后患者的免疫功能,肝组织中ＨＢｓＡｇ,ＨＢｃＡｇ变化,方法：用纯氧单仓治疗（２．５ＭＰａ,２ｈ／ｄ,１０ｄ／ｃｙｃ,ｙｃｙｃ）３０例ＣＨ,治疗前后用美国ＢＥＣＭＡＮ公司生产Ａｒｒａｙ＾ＴＭＰｒｏｔｅｉｎ Ｓｙｓｔｅｍ ３６０测全部患者静脉血白蛋白（Ａ）,γ球蛋白（γ）,ｌｇＡ,ｌｇＧ,ｌｇＭ和补体Ｃ３,Ｃ４,二次肝穿刺活检,ＡＢＣ法检测肝组织中ＨＢｓＡｇ,ＨＢｃＡｇ。     

白色念珠菌26项生化性状的检测

白色念珠菌是人兽感染常见病原,其生化性状在菌种鉴定上极为重要。为了探讨白色念珠菌的生化性状,深入分析ＶＩＴＥＫ　ａｕｔｏｍｉｃｒｏｂｉｃｓｙｓｔｅｍ（ＡＭＳ）鉴定参数设置的适用性,本文用ＶＩＴＥＫｙｅａｓｔｂｉｏｃｈｅｍｉｃａｌ　ｃａｒｄ（ＹＢＣ）测定了３１６株白色念株菌的２６项生化性状,现将结果报告如下。１　材料和方法１－１　试验菌株　３１６株白色念珠菌自临床感染患者标本中分离后用ＡＭＳ－６０进行菌种鉴定,并鉴定值为９１％～９９％,平均９６－４％。１－２　测定方法　用ＹＢＣ测定试验菌株的２６项…     

Complete amino acid sequence of murine beta 2-microglobulin: structural evidence for strain-related polymorphism.

Primary structural analyses of beta 3-microglobulin isolated from the tumor cell lines EL4.BU (derived from a C57BL/6 mouse) and C14 (derived from a BALB/c mouse) have revealed the presence of an amino acid difference at position 85 of this molecule. beta 2-Microglobulin isolated from histocompatibility antigens of EL4.BU has alanine at this position, whereas that from C14 has aspartic acid. Determination of the sequence of these molecules has employed radiochemical methodology that was developed in studies of murine histocompatibility antigens. The sequence obtained in this study is: Ile - Gln - Lys - Thr - Pro - Gln - Ile - Gln - Val - Tyr - Ser - Arg - His - Pro - Pro - Glu - Asn - Gly - Lys - Pro - Asn - Ile - Leu - Asn - Cys - Tyr - Val - Thr - Gln - Phe - His - Pro - Pro - His - Ile - Glu - Ile - Gln - Met - Leu - Lys - Asn - Gly - Lys - Lys - Ile Pro - Lys - Val - Glu - Met - Ser - Asp - Met - Ser - Phe - Ser - Lys - Asp - Trp - Ser - Phe - Tyr - Ile - Leu - Ala - His - Thr - Glu - Phe - Thr - Pro - Thr - Glu - Thr - Asp - Thr - Tyr - Ala - Cys - Arg - Val - Lys - His - Ala/Asp - Ser - Met - Ala - Glu - Pro - Lys - Thr - Val - Tyr - Trp - Asp - Arg - Asp - Met. Comparison of the sequence of murine beta 2-microglobulin to the sequences reported for the homologues from man, rabbit, and guinea pig indicate identities of 68%, 66%, and 61%, respectively.     

Molecular biology and clinical manifestation of hereditary factor VII deficiency

Inherited factor VII (FVII) deficiency is a rare autosomal recessive disorder. Mutations and polymorphisms of the FVII gene were characterized in more than 40 unrelated patients with FVII deficiency. Among the 29 different mutations, the most frequent were Ala294 Val, Ala294Val;404delC, IVS7+7, and Val281 Phe. Four novel mutations (IVS2+1G>C, Arg247 Cys, Glu265 Lys, Asp343 His) were detected. The relationships between genotypes of mutations and polymorphisms of the FVII gene, FVII deficiency, and clinical phenotype were investigated. Homozygosity of the Phe4 Leu, IVS4+1G>A, Cys135 Arg, Ala244 Val, and Ala294 Val;404delC and the double heterozygosity of Tyr68 Cys / IVS3-1G>A, Val252 Met / IVS2+5G>T, Val281 Phe / Cys135 Arg, Ala294 Val / Val281 Phe, Ala294 Val;404delC / Val281Phe, Ala294 Val;404delC / Arg152 stop, Ala294Val;404delC / Gln(-35) stop, Ala294 Val / Val252 Met, Ala294 Val / Gly156 Asp, and Thr359 Met / Asp242 His were related to clinical symptoms. Double heterozygotes for Arg247 Cys / IVS2+1G>C, Ala206 Thr / Pro303 Arg, Leu(-20) Pro / Val252 Met as well as IVS7+7 /Ala294 Val, IVS7+7 /Ala206 Thr, and IVS7+7 / Met298 Ile were asymptomatic. The clinical symptomatology is rather poor in correlation with the FVII activity. Concerning the clinical phanotype, a correlation seems to exist between specific mutations and clinical symptoms.     

用Y.pestis接种某些植物的研究

本研究采用鼠疫弱毒株０６１号对大豆，小麦，兴安胡枝子，紫苜蓿，青蒿等进行接种，在不同时期从大豆组织中分离到目的菌，并发现其菌落形态由Ｒ型向Ｓ型改变，其营养需求也发生了明显的变化。     

The hyperglycemic neuropeptide of the terrestrial isopod, Porcellio dilatatus. I. Isolation and characterization

From isolated sinus glands of Porcellio dilatatus, a hyperglycemic neuropeptide (CHH) was purified by means of a single, two-step purification procedure which consisted of gel chromatography on Sephadex G-50, followed by high-performance liquid chromatography. The 5800- to 6100-Da peptide contains 50-52 amino acids residues. The amino acid composition is (Pro, Ile)1; His1-2; (Thr, Ser, Gly, Met, Tyr)2; (Val, Phe, Lys)3; (Ala, 1/2Cys, Leu, Arg)4; Glx5; Asx7; Trp, n.d. The amino acid composition differs from that of two decapod CHHs analyzed thus far. The N-terminus is blocked. The neuropeptide exhibits little or no interspecific hyperglycemic effect in the brachyuran, Uca pugilator, and its cross-reactivity and potency in the RIA for Carcinus-CHH is very low.     

Congenital coagulation factor X deficiency: Genetic analysis of five patients and functional characterization of mutant factor X proteins

Congenital factor X (FX) deficiency is a rare bleeding disorder that is inherited as an autosomal recessive trait. In this study, a genetic analysis of the FX gene was performed in five families with this disorder. Four heterozygous mutations [p.Gly154Arg, p.Val236Met, p.Gly263Val and p.Arg387Cys] and one pair of compound heterozygous FX gene mutations consisting of p.Gly406Ser and p.Val424Phe were identified. Mutant FX proteins containing the identified amino acid substitutions were also expressed in cultured cells. These proteins were analysed by enzyme‐linked immunosorbent assay and pulse‐chase experiments. The results demonstrated normal intracellular synthesis and extracellular secretion of mutant FX proteins carrying the p.Val236Met, p.Arg387Cys and p.Gly406Ser amino acid substitutions. However, the results also showed that the p.Gly154Arg, p.Gly263Val and p.Val424Phe proteins were secreted less efficiently than the wild‐type protein, although they were synthesized normally in the cell. Collectively, these observations suggest that the amino acid substitutions p.Gly154Arg, p.Gly263Val and p.Val424Phe induce protein misfolding, leading to the intracellular degradation of many FX proteins containing any of these mutations, and ultimately to the development of FX deficiency. On the other hand, for the p.Val236Met, p.Arg387Cys and p.Gly406Ser mutant proteins, we hypothesize that secreted FX proteins have impaired coagulant activities due to functional defects caused by these amino acid substitutions.     

Site of aminoacylation of tRNAs from Escherichia coli with respect to the 2'- or 3'-hydroxyl group of the terminal adenosine.

A method is presented by which the site of primary attachment of the amino acids with respect to the 2'- or 3'-hydroxyl group of the terminal adenosine of E. coli tRNAs can be determined. It is found that the aminoacyl-tRNA synthetases (EC 6.1.1.-) with specificity for Arg, Asn, Ile, Leu, Met, Phe, Thr, Trp, and Val attach the amino acid to the 2'-position; those with specificity for Gly, His, Lys, and Ser attach the amino acid to the 3'-position; and that Tyr and Cys can be enzymatically attached to both the 2'- and 3'-positions. Together with previous experiments on yeast aminoacyl-tRNA synthetases, it is now shown that the specificity for one particular hydroxyl group is preserved during the evolution from prokaryotic to eukaryotic systems.     

左氧氟沙星联合CD_(25)单克隆抗体治疗小鼠结核病的初步研究

目的 研究CD_4~+CD_(25)~+调节性T细胞(Treg细胞)消除与左氧氟沙星联合治疗对小鼠结核病细胞免疫作用的影响.方法 3～4周龄C57BL/6雌性小鼠76只,体重17～19 g,随机数字表法分为对照组、CD_(25)单克隆抗体(PC61)消除组、左氧氟沙星组和联合治疗组,每组19只.PC61消除组和联合治疗组小鼠腹腔注射含50 μg PC61的PBS,0.2 ml/只,对照组和左氧氟沙星组小鼠腹腔注射含50μg小鼠IgG同型对照的PBS,0.2ml/只.全部小鼠3 d后尾静脉注射0.1 ml的H_(37)Rv(1×10~6 CFU)造模,左氧氟沙星组和联合治疗组小鼠于攻毒后第2天每只给予左氧氟沙星200 mg·kg~(-1)·d~(-1)连续灌胃45 d.分析小鼠体内不同组织中Treg细胞百分率及其对特异性细胞免疫、肺组织病理变化的影响.应用单因素方差分析对4组连续变量进行检验,组间比较采用q检验,组内不同时间点比较采用t检验.结果 感染MTB第10天和第30天小鼠脾细胞中Treg细胞百分率:对照组分别为(30±4)%和(17.3±1.6)%,PC61消除组分别为(21±4)%和(16.1±1.3)%,左氧氟沙星组分别为(44±6)%和(24.7±2.0)%,联合治疗组分别为(24±3)%和(10.4±1.0)%,除第10天联合治疗组与PC61消除组比较无明显差别外,其他各组间比较均差异有统计学意义(q值为3.62～5.56,均P<0.05).联合治疗组小鼠的肺组织病变较轻,死亡数量较少.结论 Treg细胞消除联合左氧氟沙星治疗可促进结核病小鼠的特异性细胞免疫,使肺内病变有所改善,并可降低小鼠的病死率.     

Sequences of the recA gene and protein.

We have determined the nucleotide sequence of the recA gene of Escherichia coli; this permits the formulation of the primary structure for the recA protein. This structure is consistent with the amino acid composition of the tryptic peptides obtained from the recA protein. The coding region of the recA gene has 1059 base pairs, which specify 352 amino acids. The recA protein has alanine and phenylalanine as its NH2- and COOH-terminal amino acids, respectively, and has the following amino acid composition: Cys3 Asp20 Asn15 Met9 Thr17 Ser20 Glu30 Gln13 Pro10 Gly35 Ala38 Val22 Ile27 Leu31 Tyr7 Phe10 His2Lys27 Trp2 Arg14. Of the three cysteine residues, only two can be alkylated under reducing and denaturing conditions. The molecular weight of the recA polypeptide is 37,842.     

山东恙虫病立克次体生物学及免疫学性质的研究

本文报告了山东恙虫病立克次体人株生物学及免疫学性质的初步研究。所研究3株中的1株感染小鼠发病不典型,极少致死;另两株感染小鼠发病典型,小鼠呈规律性的死亡,易适应于鸡胚,对小鼠毒力LD_(50)为3.4。感染小鼠之IgG抗体出现于接种后的第13天,于接种后的第3～4周抗体水平达峰值。上述分离株与Karp毒株间存在较强的交叉保护作用。酶免疫染色血清学反应结果指出,与Gilliam株很一致,可能属Gilliam同一血清型。