寻常型银屑病血清新喋呤水平的测定

用酶联免疫分析法测定３０例寻常型银屑病患者的血清新喋呤水平。结果表明，患者组的血清新喋呤水平显著高于对照组（Ｐ＜０．００１），进行期显著高于静止期（Ｐ＜０．００１），治疗前显著高于治疗后（Ｐ＜０．００１），且新喋呤水平随病情活动而变化，与皮损严重程度呈显著正相关关系（ｒ＝０．７９５６，Ｐ＜０．００１）。提示银屑病的发病与细胞免疫的激活有关，新喋呤水平测定可作为监测该病活动和治疗效果的指标。     

寻常性银屑病患者血清中MIF和VEGF的表达

目的探讨巨噬细胞移动抑制因子(MIF)和血管内皮生长因子(VEGF)与寻常性银屑病的关系。方法用人MIF,VEGF的ELISA试剂盒分别测定40例银屑病患者和17例正常人血清中MIF和VEGF值。结果寻常性银屑病患者MIF水平明显高于正常对照组(P<0.01),进行期患者高于静止期(P<0.01);进行期寻常性银屑病患者VEGF水平高于静止期(P<0.01)及正常对照组;寻常性银屑病患者血清中MIF水平与PASI评分成正相关,而VEGF水平与PASI评分无相关性(P>0.05);寻常性银屑病患者血清中MIF与VEGF水平呈正相关性(P<0.05)。结论寻常性银屑病患者血清MIF和VEGF水平明显改变,二者可能在寻常性银屑病发病机制中起重要作用。     

银屑病患者皮损中VEGF受体FLT-1和KDR的表达

目的:检测VEGF受体FLT-1与KDR在寻常型银屑病皮损中的表达水平,探讨其在银屑病发病中的意义。方法:应用免疫组化SABC法,检测37例寻常型银屑病患者皮损和37例正常人皮肤组织FLT-1蛋白、KDR蛋白的表达。结果:寻常型银屑病皮损处的FLT-1蛋白和KDR蛋白的表达水平明显强于正常对照组(P<0.001);KDR的表达强度与寻常型银屑病严重程度指数PASI评分有显著正相关(r=0.93,P<0.001),且KDR的表达强度高于FLT-1的表达强度(P<0.05)。结论:VEGF的两种受体FLT-1与KDR在银屑病新生血管形成中具有重要作用,其中KDR起主要作用。通过阻断VEGF受体KDR以抗血管生成,可望为治疗银屑病提供一种新的有效方法。     

NB-UVB治疗前后银屑病皮损中VEGF与CD106的表达

用免疫组化的方法检测VEGF与CD106在26例紫外线治疗前后银屑病患者皮损中的表达,20例正常人作对照,探讨它们在银屑病发病机制中的作用.NB-UVB治疗前患者皮损中VEGF与CD106的阳性表达率高于治疗后组,(均P＜0.01).治疗前组阳性表达率高于正常对照组(均P＜0.01),治疗后两者的阳性表达率与正常对照组差异无统计学意义(均P＞0.05).治疗前后患者皮损VEGF和CD106的表达呈正相关(P=0.015).VEGF和CD106在银屑病患者皮损中过表达,在促进银屑病患者皮损中血管生成中发挥作用并互相影响.紫外线照射治疗银屑病有效,可能是通过降低角质形成细胞促血管生成新生因子的表达而间接发挥抗血管生成作用.     

关节病型银屑病患者外周血中血管内皮生长因子(VEGF)水平的动态观测

目的：检测关节型银屑病患者外外周血中VEGF水平的改变，探讨其与PASI评分、治疗前后的关系。方法：采用双抗体夹心酶联免疫技术（ELISA）检测23例关节病型银屑病患者治疗前、中、治疗后血清中VEGF水平。另检测了23例正常人血清中的VEGF水平作为对照组。结果：关节病型银屑病患者血清中VEGF水平较正常对照组显著增高（P〈0．001），治疗前后患者血清中VEGF水平有显著差异（P〈0．05）。结论：VEGF在关节病型银屑病发病机制中起重要作用。     

NB—UVB治疗前后银屑病皮损中VEGF与CD106的表达

用免疫组化的方法检On．0VEGF与CD106在26例紫外线治疗前后银屑病患者皮损中的表达,20例正常人作对照,探讨它们在银屑病发病机制中的作用。NB—UVB治疗前患者皮损中VEGF与CD106的阳性表达率高于治疗后组,（均P〈0．01）。治疗前组阳性表达率高于正常对照组（均P〈0．01）,治疗后两者的阳性表达率与正常对照组差异无统计学意义（均尸〉0．05）。治疗前后患者皮损VEGF和CD106的表达呈正相关（P=0．015）。VEGF和CD106在银屑病患者皮损中过表达,在促进银屑病患者皮损中血管生成中发挥作用并互相影响。紫外线照射治疗银屑病有效,可能是通过降低角质形成细胞促血管生成新生因子的表达而间接发挥抗血管生成作用。     

光化学疗法对银屑病患者皮损中微血管密度和血清血管内皮生长因子水平的影响

目的:了解光化学疗法(PUVA)对银屑病患者皮损中微血管密度(MVD)和血清血管内皮生长因子(VEGF)水平的影响。方法:用血管内皮细胞特异性标志物CD34抗体免疫组化法检测银屑病患者PUVA治疗前后皮损中的MVD,用ELISA方法检测患者PUVA治疗前后血清VEGF的水平。结果:银屑病患者PUVA治疗前的MVD明显高于治疗后及正常对照组P均<0.01。银屑病患者PUVA治疗前的血清VEGF水平明显高于治疗后及正常对照组P均<0.01。结论:PUVA可能通过抗血管新生作用来治疗银屑病。     

寻常型银屑病患者血清中MMP-9与VEGF的表达

目的:检测基质金属蛋白酶-9(MMP-9)与血管内皮生长因子(VEGF)在寻常型银屑病(PV)患者血清中的表达.方法:采用ELISA法检测42例PV血清中MMP-9和VEGF水平,以30名正常人做对照.结果:PV患者血清中MMP-9和VEGF的水平均显著高于对照组(P<0.01);进行期患者显著高于静止期患者(P<0.05);血清浓度变化与PV严重程度指数PASI呈正相关.结论:PV患者血清MMP-9和VEGF水平有助于对PV病情的判断.     

银屑病患者角质形成细胞VEGF表达的研究

目的　研究银屑病发病与血管内皮生长因子 (VEGF)的关系 ,探讨银屑病可能的发病机制。方法　①用免疫组化法检测银屑病患者皮损和非皮损处皮肤、正常健康人皮肤及体外培养的银屑病患者和正常人角质形成细胞(KC)VEGF的表达 ;②用双抗体夹心ELISA法检测银屑病患者及正常人KC培养上清液中VEGF含量。结果　①银屑病皮损处VEGF表达明显高于非皮损处和正常人皮肤 (P均 <0 .0 0 1) ,非皮损处与正常人皮肤VEGF表达也有显著性差异 (P <0 .0 5 ) ;②体外培养的银屑病皮损处和非皮损处KCVEGF表达明显高于正常人 (P均 <0 .0 0 1) ;银屑病皮损处KC与非皮损处KC相比VEGF表达也有显著性差异 (P <0 .0 5 ) )。结论　VEGF可能参与银屑病的发病。     

血管内皮生长因子在寻常型银屑病患者中医辨证分型皮损和血清中的表达

目的探讨血管内皮生长因子(VEGF)在寻常型银屑病患者中医辨证分型(血热型、血燥型和血瘀型)皮损和血清中的表达。方法采用免疫组化法和酶联免疫吸附法(ELISA法)对60例寻常型银屑病患者(中医辨证分型)皮损处及血清中VEGF的水平进行了检测。结果皮损处VEGF水平为血热型组>血燥型组及血瘀型组;血清中VEGF的水平为血热型组>血燥型组>血瘀型组。结论对寻常型银屑病患者皮损处及血清中VEGF水平的检测有可能用于血热型银屑病与非血热型银屑病的中医辨证分型。     

维甲酸诱导前后HB-EGF在银屑病皮损中的变化

目的　研究维甲酸在进行期寻常型银屑病中的治疗机制。方法　用免疫组织化学的方法检测维甲酸诱导前后HB GFE在进行期银屑病皮损中的表达。结果　在银屑病皮损中 ,全层几乎无HB EGF的表达 ,维甲酸诱导后 10天可见HB EGF不仅表达于基底层 (10 0 .0 % ) ,且以灶状表达于基底上层 (77.8% )。结论　维甲酸通过上调银屑病表皮中HB EGF的表达 ,抑制角质形成细胞的增殖并诱导凋亡     

银屑病与血清瘦素水平的研究

目的 探讨血清瘦素水平与不同类型银屑病的发生及其病情的相关性。方法 51例银屑病患者及年龄、性别、体质指数(BMI)与之均衡的正常人对照50例,采用放射免疫技术检测其血清瘦素水平。结果 银屑病患者血清瘦素水平(7.62±4.32ng/mL)较正常人对照(5.40±3.14ng/mL)显著增高(P=0.004);血清瘦素水平可见显著的性别差异(P<0.001);寻常性银屑病与正常人对照比较,患者血清瘦素水平(7.64±3.90ng/mL)显著增高(P=0.006),尤其进行期患者及无银屑病家族史患者血清瘦素水平(8.29±3.85ng/mL及8.41±3.70ng/mL)显著增高(P=0.002及P=0.001);与正常人对照比较,脓疱性银屑病血清瘦素水平(11.72±6.45ng/mL)显著增高(P<0.001),而红皮病性及关节病性银屑病差异无统计学意义(P>0.05)。结论 瘦素可能在银屑病的发生及发展过程中起作用。     

Erythroid Disturbances Before and After Treatment of Portuguese Psoriasis Vulgaris Patients

Background: A few studies in psoriasis vulgaris patients have reported changes suggesting red blood cell (RBC) damage is linked to neutrophil activation, oxidative stress, and psoriasis worsening. Objective: The aim of this study was to evaluate erythroid disturbances in Portuguese psoriasis vulgaris patients, before, during, and after treatment. Methods: Across-sectional study (n = 73 patients vs 40 healthy control subjects) followed by a longitudinal study (n = 47 patients)was performed, with assessments before, and at 3, 6, and 12weeks of therapy (10 patients started topical treatment, 17 narrow-band UVB, and 20 photochemotherapy [psoralen plus UVA; PUVA]). Evaluations included hematologic data, total bilirubin levels,membrane-bound hemoglobin (MBH),membrane protein band 3 profile, total plasma antioxidant status (TAS), lipid peroxidation (thiobarbituric acid [TBA] assay), elastase, lactoferrin, and C-reactive protein (CRP). Results: Before treatment, patients presented with higher leukocyte/neutrophil and reticulocyte counts, elastase, lactoferrin, TBA, TBA/TAS, reticulocyte production index, total bilirubin and MBH values, lower RBC and hematocrit, higher percentages of high-molecular-weight aggregates, and lower percentages of band 3 monomer. After treatment, we observed a reversal in most of the parameters. However, patients still presented with values suggestive of accelerated RBC damage, removal, and production, as most of the parameters were still higher than those in the control group; the same occurred with CRP. Conclusion: Our data suggest that psoriasis vulgaris triggers an inflammatory response, with release of acutephase reactants, reactive oxygen species, cationic proteins, and proteases, leading to enhanced RBC damage/ aging and, ultimately, to enhanced RBC removal. These assumptions were strengthened by the observation that, with treatment, all of these changeswere reversed, the inflammation was reduced, the production of reticulocytes was increased, and the RBCs presented changes usually observed in younger/less damaged RBCs. These erythroid changes were enhanced with PUVA therapy, probably due to the more pronounced clearing of the lesions, as suggested by Psoriasis Area and Severity Index (PASI) scores. Finally, after treatment, a residual inflammation still persisted that might contribute to the observed erythroid disturbances.     

银屑病患者血浆β—内啡肽测定及其临床意义

为了探讨β－内啡肽（β－ＥＰ）在银屑病发病中的作用机理，采用放射免疫分析法对５１例银屑病患者外周血β－ＥＰ水平进行了检测，并与３２例健康体检者对照。结果显示：银屑病患者血浆β－ＥＰ水平明显高于对照组，即使达到临床治愈时，β－ＥＰ水平有所下降，但仍高于对照组；发病前有诱因者较无诱因者β－ＥＰ水平有增高趋势，但无统计学意义；皮损瘙痒的强度与血浆β－ＥＰ水平之间呈显著负相关。结论：β－ＥＰ参与银屑病的发病过程，其分泌既受中枢神经系统控制，又受皮损内炎症反应的影响，β－ＥＰ水平与皮损内炎症反应之间可能互为因果     

寻常性银屑病血清MCP-1的检测

目的 初步探讨单核细胞趋化因子-1（monocyte chemoattractant protein-1,MCP-1)在银屑病发病机制中的作用。方法 分离处于不同病期的寻常性银屑病患者外周血清，通过酶联免疫吸附（ELISA）法检测银屑病患者血清中MCP-1的水平；同时比较了不同病期中血清MCP-1水平。结果 银屑病患者血清中MCP-1水平明显高于健康对照组，但MCP-1的水平并不随病期的变化而变化。结论 银屑病外周血MCP-1的高表达可能与银屑病的发病有关。     

Tissue and Serum Inflammatory Cytokine Levels in Korean Psoriasis Patients: A Comparison between Plaque and Guttate Psoriasis

BackgroundThe phenotypic heterogeneity of psoriasis could be explained by the alternate activation of either T-helper (Th)-1- or Th-17-related cytokines. However, evidence directly supporting this hypothesis is scarce.ObjectiveTo characterize the expression of Th-1- and Th-17-related cytokines according to the morphological psoriasis phenotype: guttate vs. plaque.MethodsIn this study, we enrolled 68 patients exhibiting either guttate or plaque psoriasis, and 10 healthy controls. To avoid age-related bias, age matching was performed for each group. Circulating levels of interferon (IFN)-γ, interleukin (IL)-1RA, IL-2, IL-12p40, IL-17A, IL-22, and IL-23 were measured with an enzyme-linked immunosorbent assay (ELISA). Psoriasis-affected tissue was obtained through biopsy sampling from the eight patients who exhibited the most typical morphology. Levels of IL-1RA, IL-12p40, IL-17, IL-22, and IL-23 in the psoriasis tissue samples were measured with western blot analysis.ResultsELISAs of the serum samples showed higher levels of inflammatory cytokines such as IL-1RA, IL-2, IL-23, and IFN-γ in patients with psoriasis than in healthy controls. However, the inflammatory cytokine levels did not differ significantly between guttate and plaque psoriasis patients. Western blot analysis of psoriatic tissue revealed higher protein levels of Th-1- and Th-17-related cytokines in patients than in healthy controls. The levels of IL-12p40 and IL-23 were unexpectedly higher in plaque tissue than in guttate tissue.ConclusionThe morphological phenotype of psoriasis does not appear to be determined by a specific activation of either the Th-1 or Th-17 pathway. Rather, the cytokine profile influences disease activity and is altered according to the status of the lesion (early or chronic).     

补骨脂注射液治疗寻常性银屑病临床疗效观察

目的观察补骨脂治疗寻常性银屑病的临床疗效及安全性。方法对照组口服克银丸、维生素E,外用丁酸氢化可的松霜,治疗组在对照组基础上,加用补骨脂注射液,1个月后判定疗效。结果治疗组治疗前后的PASI评分分别为12.12±2.61,2.87±0.92,对照组为13.00±2.54,5.67±1.01,两组治疗前后评分差异有显著性(P均<0.001);治疗组有效率为66.67%,对照组为40.00%,两组有效率差异有显著性(P<0.05)。结论补骨脂治疗银屑病安全、有效。     

银屑病性关节炎的治疗进展

银屑病性关节炎是一种难治性疾病。过去治疗一直局限在非甾体类解热镇痛药和免疫抑制剂,治疗效果不满意。近年来国际上对其治疗的研究已主要集中在分子水平上,如抗肿瘤坏死因子(TNF)抗体,T细胞靶向药物等。本文将在回顾传统治疗的基础上,重点介绍其治疗新进展。     

银屑病皮损中Survivin、VEGF和Ang—2的表达

目的：检测存活素（Survivin）、血管内皮生长因子（VEGF）及血管生成素-2（Ang-2）等因子在寻常型银屑病（PV）皮损中的表达。方法：用免疫组化方法检测30例PV皮损及30例正常皮肤组织中Survivin、VEGF及Ang-2的表达。结果：PV皮损中Survivin、VEGF及Ang-2的表达明显强于正常皮肤组织。Survivin,VEGF和Ang-2之间的表达呈正相关。结论：Survivin、VEGF及Ang-2共同参与了银屑病新生血管的生成过程。