Use of fly screens to reduce Campylobacter spp. introduction in broiler houses

Fly screens that prevented influx of flies in 20 broiler houses during the summer of 2006 in Denmark caused a decrease in Campylobacter spp.-positive flocks from 51.4% in control houses to 15.4% in case houses. A proportional reduction in the incidence of chicken-borne campylobacteriosis can be expected by comprehensive intervention against flies in broiler production houses.     

Presence of Salmonella spp. and Campylobacter spp. in shellfish.

Bivalve molluscs, (cockles, mussels, scallops and oysters) were examined according to EC shellfish bed classification regulations for faecal coliforms, Escherichia coli and salmonella, and for coliforms and campylobacter which are not specified by these regulations. Salmonella serotypes were detected in 8% of 433 molluscs. Seven salmonella isolations (2%) were made from category A beds, nominally suitable for immediate consumption according to E. coli counts. A higher percentage of salmonella isolates (6%) was detected in shellfish which require relaying or depuration prior to eating. In another survey, thermophilic Campylobacter spp. were found in 42% of 380 shellfish. These findings show bed classification on the basis of indicator organisms alone is not sufficient to assure the absence of bacterial, and no doubt viral, pathogens. Depuration and end product specifications which require the absence of salmonellae are an essential part of these regulations. Microbiologists may wish to consider whether tests for pathogens such as salmonella and campylobacter should be included when determining the suitability of shellfish for human consumption.     

Analysis of simultaneous space-time clusters of Campylobacter spp. in humans and in broiler flocks using a multiple dataset approach

Background

Campylobacteriosis is the most frequently reported zoonosis in the EU and the epidemiology of sporadic campylobacteriosis, especially the routes of transmission, is to a great extent unclear. Poultry easily become colonised with Campylobacter spp., being symptom-less intestinal carriers. Earlier it was estimated that internationally between 50% and 80% of the cases could be attributed to chicken as a reservoir. In a Norwegian surveillance programme all broiler flocks under 50 days of age were tested for Campylobacter spp. The aim of the current study was to identify simultaneous local space-time clusters each year from 2002 to 2007 for human cases of campylobacteriosis and for broiler flocks testing positive for Campylobacter spp. using a multivariate spatial scan statistic method. A cluster occurring simultaneously in humans and broilers could indicate the presence of common factors associated with the dissemination of Campylobacter spp. for both humans and broilers.

Results

Local space-time clusters of humans and broilers positive for Campylobacter spp. occurring simultaneously were identified in all investigated years. All clusters but one were identified from May to August. Some municipalities were included in clusters all years.

Conclusions

The simultaneous occurrence of clusters of humans and broilers positive for Campylobacter spp. combined with the knowledge that poultry meat has a nation-wide distribution indicates that campylobacteriosis cases might also be caused by other risk factors than consumption and handling of poultry meat. Broiler farms that are positive could contaminate the environment with further spread to new broiler farms or to humans living in the area and local environmental factors, such as climate, might influence the spread of Campylobacter spp. in an area. Further studies to clarify the role of such factors are needed.     

人类空肠弯曲菌感染的流行病学

八十年代以来,随着对空肠弯曲菌感染研究的不断深入,有关本病的流行病学也获得了较全面的认识[1]。     

Flies and Campylobacter infection of broiler flocks

A total of 8.2% of flies caught outside a broiler house in Denmark had the potential to transmit Campylobacter jejuni to chickens, and hundreds of flies per day passed through the ventilation system into the broiler house. Our study suggests that flies may be an important source of Campylobacter infection of broiler flocks in summer.     

Surveillance of Salmonella spp. and Campylobacter spp. in poultry production flocks in The Netherlands

In The Netherlands, a national programme for the surveillance of zoonotic bacteria in farm animals has been operative since 1997. We describe the results of the surveillance of Salmonella spp. in flocks of laying hens and broilers and of Campylobacter spp. in broiler flocks in the period 1999-2002. The prevalence of Salmonella spp. in laying-hen flocks has significantly decreased from 21.1% in 1999 to 13.4% in 2002. This decreasing trend might indicate that the control measures taken by the poultry industry were effective. S. Enteritidis was the predominant serovar in laying hens accounting for one third of the positive flocks. Although prevalence estimates for Salmonella spp. in broiler flocks did not yield a significant decreasing trend in 1999-2002, a decrease in Salmonella prevalence to 11% was measured in 2002. During the study period, S. Paratyphi B var. Java emerged in broilers to become the predominant serovar in 2002 accounting for one third of the positive flocks. The prevalence of Campylobacter spp. in broiler flocks did not increase nor decrease continuously between 1999 and 2002, which roughly corresponds with the monitoring results from the poultry industry. In this period, the estimated flock prevalence roughly averaged around 20%, with C. jejuni being the predominant species. The approach of monitoring presented in this paper can serve as a blueprint for monitoring schemes in farm animal populations to be developed in the context of the EC Zoonoses Directive.     

Experimental colonization of broiler chicks with Campylobacter jejuni

Minimal colonization inocula for two broiler strains of Campylobacter jejuni were determined in broiler chicks aged 2-3 days and 2 weeks. Individually housed chicks were exposed to a single oral or cloacal challenge. Diarrhoeal symptoms were absent in all 380 chicks included in the study. Chick susceptibility to the two C. jejuni strains varied. Colonization was effected by less than 10(2)-10(4) colony forming units (c.f.u.) via cloacal challenge and 10(4)-10(6) c.f.u. via the oral route. Colonization inocula for 2- to 3-day and 2-week-old chicks were similar. Treatment of 1-day-old chicks with fresh adult caecal flora or an anaerobic broth culture of adult caecal flora did not inhibit colonization after challenge with low-dose C. jejuni. Susceptible chicks were colonized rapidly. C. jejuni was detected in 167 of 189 (88%) colonized chicks within 3 days of challenge and persisted during the 2-week monitoring period. Our data suggest that colonization of broiler chicks with C. jejuni is effected more easily by the cloacal than the oral route and is independent of age.     

Ciprofloxacin-Resistant Campylobacter spp. in Retail Chicken,Western Canada

During 2005–2010, the Canadian Integrated Program for Antimicrobial Resistance Surveillance identified increased prevalence of ciprofloxacin (a fluororquinolone) resistance among Campylobacter isolates from retail chicken in British Columbia (4%–17%) and Saskatchewan (6%–11%), Canada. Fluoroquinolones are critically important to human medicine and are not labeled for use in poultry in Canada.     

Campylobacter spp. in Icelandic poultry operations and human disease

We describe the observed relationship of campylobacter in poultry operations to human cases in a closed environment. During 1999 in Iceland, domestic cases of campylobacteriosis reached peak levels at 116/100,000 and in 2000 dropped to 33/100,000. Approximately 62% of broiler carcass rinses were contaminated with Campylobacter spp. in 1999. During 2000, only 15% of the broiler flocks tested Campylobacter spp. positive. In 2000, carcasses from flocks which tested positive on the farms at 4 weeks of age were subsequently frozen prior to distribution. We suggest that public education, enhanced on-farm biological security measures, carcass freezing and other unidentified factors, such as variations in weather, contributed to the large reduction in poultry-borne campylobacteriosis. There is no immediate basis for assigning credit to any specific intervention. We continue to seek additional information to understand the decline in campylobacteriosis and to create a risk assessment model for Campylobacter spp. transmission through this well defined system.     

The isolation of Campylobacter spp. from modified atmosphere packaged foods

Foodborne illness caused by Campylobacter spp. is a major public health concern in many countries. This study investigated the prevalence of Campylobacter spp. in modified atmosphere packaged (MAP) foods such as fresh meat and vegetables in order to assess the risk to human health from foods packaged using this new technology, the application of which is increasing rapidly. Samples of MAP chicken meat, MAP turkey meat, MAP bacon and MAP mixed salad vegetables from two supermarkets were tested for the presence of the organism in two types of samples; those tested immediately on purchase and those stored at 4 C (+/- 2 C) and subsequently tested on the 'use by' date stated on the package. Campylobacter spp. were found to be present in a proportion of all MAP products tested: 90% of MAP turkey samples, 71.25% of MAP chicken samples, 67.5% of cured bacon samples and 22.2% of mixed salad vegetables samples were found to be contaminated with Campylobacter spp. Viable counts indicated that the level of infection varied both between food products and between different packages of the same product. Turkey meat was found to have the highest numbers of organisms present (5.2 102-3.3 103 cfu g-1) compared with 0.2-7.2 102 cfu g-1 for chicken meat and 0.1-3.1 102 g-1 for bacon. The highest count obtained from one sample of salad vegetables was 1.7 102 cfu g-1 with the majority (16 out of a total of 20 positive samples) being less than 0.8 102 cfu g-1 and all 12 of the positive samples tested on date of purchase having viable counts of less than 0.1 102 cfu g-1.     

Integronlike structures in Campylobacter spp. of human and animal origin

Resistance to antimicrobial agents used to treat severe Campylobacter spp. gastroenteritis is increasing worldwide. We assessed the antimicrobial resistance patterns of Campylobacter spp. isolates of human and animal origin. More than half (n = 32) were resistant to sulphonamide, a feature known to be associated with the presence of integrons. Analysis of these integrons will further our understanding of Campylobacter spp. epidemiology.     

The colonization of broiler chickens with Campylobacter jejuni: some epidemiological investigations.

Between June 1990 and July 1991, broiler chickens from 49 flocks from 23 farms were examined for the carriage of Campylobacter jejuni at slaughter. Thirty-seven flocks (76%) were campylobacter-positive. Prevalence of campylobacter-colonization was not associated with any of a variety of factors such as water source and broiler house floor structure. There was also no apparent seasonal variation in carriage. Investigations on one farm indicated that dipping boots in disinfectant before workers entered broiler houses either delayed or prevented colonization with C. jejuni.     

Salmonella and Campylobacter spp. in northern elephant seals, California

Campylobacter and Salmonella spp. prevalence and antimicrobial drug sensitivity were determined in northern elephant seals that had not entered the water and seals that were stranded on the California coast. Stranded seals had a higher prevalence of pathogenic bacteria, possibly from terrestrial sources, which were more likely to be resistant.     

Antimicrobial growth promoters and Salmonella spp., Campylobacter spp. in poultry and swine,Denmark

The use of antimicrobial growth promoters in Danish food animal production was discontinued in 1998. Contrary to concerns that pathogen load would increase; we found a significant decrease in Salmonella in broilers, swine, pork, and chicken meat and no change in the prevalence of Campylobacter in broilers.     

Campylobacter spp. in waters of three main western Pomerania water bodies

Campylobacters are responsible for increasing numbers of gastroenteritis cases in humans as well as miscarriages and diarrhea in farm and domestic animals. Surface waters are potential reservoirs and transmitting vehicles for these bacteria.

Subject of analysis were surface water samples collected from the River Odra, Szczecin Lagoon and the Pomeranian Bay in monthly or bimonthly intervals, starting from April 1998. Analysis directed on campylobacters included enrichment in Preston broth prior to plating on CCDA isolation medium and identification, to the species level, by the api CAMPY tests. The detection level of the method was 1 CFU/10 ml. Numbers of total and faecal coliforms were counted according to the national standards. In addition water temperature and water saturation with oxygen were measured.

Presence of Campylobacter spp., at the level detectable by the method applied, was confirmed in 19.7 % of the Odra River, 5.6 % of the Szczecin Lagoon and 0 % of the Pomeranian Bay surface water samples. The contamination level of the Campylobacter – positive surface water samples did not exceed 10 CFU/ml. Isolation frequency depended on water purity class, counts of total and faecal coliforms and sampling site (p < 0.01). Species dominating in the River Odra and Szczecin Lagoon surface waters were C. jejuni ssp. jejuni and C. coli, respectively     

A one-year study of campylobacter carriage by individual Danish broiler chickens as the basis for selection of Campylobacter spp. strains for a chicken infection model

From February 1999 to February 2000, 1,250 individual broiler chickens representing 125 broiler flocks originating from 62 broiler farms in Denmark were screened for campylobacter carriage. Every month, 10 flocks were tested for campylobacter carriage. The swabs were tested individually and as a pooled sample representing the flocks. Campylobacter spp. carriage was detected from 512 (40.9%) broiler chickens originating from 63 (50.4%) positive flocks. Campylobacter carriage by both individual chickens and flocks showed seasonal variation. Campylobacter jejuni was the dominant species (95.5%). Campylobacter isolates were typed using Penner heat-stable serotyping and flaA-typing methods. Data of campylobacter carriage by individual chickens and data generated by the use of different typing methods contributed to a better understanding of the dynamics of campylobacter infection within the broiler flocks. C. jejuni Penner heat-stable serotype HS2, flaA-type 1 was the most common type found in Danish broiler chickens.     

Extended sequence typing of Campylobacter spp., United Kingdom

Supplementing Campylobacter spp. multilocus sequence typing with nucleotide sequence typing of 3 antigen genes increased the discriminatory index achieved from 0.975 to 0.992 among 620 clinical isolates from Oxfordshire, United Kingdom. This enhanced typing scheme enabled identification of clusters and retained data required for long-range epidemiologic comparisons of isolates.