联合检测血清胃蛋白酶原、胃泌素-17在萎缩性胃炎及胃癌筛查中的价值

目的: 通过测定不同胃疾病的血清胃蛋白酶原(PG)Ⅰ、PGⅡ水平和二者比值(PGR)以及胃泌素-17(G-17)水平,探讨PG、G-17作为萎缩性胃炎和胃癌筛查指标的价值.方法: 选取2012年8月至2013年10月行胃镜检查的门诊及住院患者共278例,根据胃镜及病理结果分为4组,包括非萎缩性胃炎组105例,萎缩性胃炎组42例,胃癌组64例和胃溃疡组67例.用酶联免疫吸附试验定量检测每例血清PGⅠ、PGⅡ和G-17水平,并计算PGR值.结果: 萎缩性胃炎组及胃癌组PGⅠ水平和PGR均较非萎缩性胃炎组明显降低(P< 0.01),G-17水平明显升高(P< 0.01);胃癌组PGⅠ水平和PGR均较萎缩性胃炎组明显降低(P< 0.01),G-17水平差异无统计学意义(P >0.05).胃癌组中,进展期胃癌血清PGⅠ水平、PGR明显低于早期胃癌(P< 0.01),G-17水平差异无统计学意义(P >0.05).结论: 血清PGⅠ、PGR降低及G-17升高提示了萎缩性胃炎和胃癌的高危风险,联合检测血清PG和G-17可以作为胃萎缩及胃癌的筛查手段,为是否需行进一步胃镜检查提供积极参考.     

血清胃泌素联合胃蛋白酶原检测对萎缩性胃窦炎及胃癌的诊断价值

目的:探讨血清胃泌素联合胃蛋白酶原检测对萎缩性胃窦炎及胃癌的诊断价值。方法:选择本院门诊及住院符合入选标准的182例患者,据胃镜及病理检查结果分为非萎缩性胃炎组67例,萎缩性胃窦炎组66例,胃癌组49例。采用酶联免疫吸附法检测各组血清中胃泌素-17(gastrin-17,G-17)、胃蛋白酶原Ⅰ(pepsinogenⅠ,PGⅠ)及胃蛋白酶原Ⅱ(pepsinogenⅡ,PGⅡ)水平,计算胃蛋白酶原Ⅰ/胃蛋白酶原Ⅱ(pepsinogen I/pepsinogen II,PGR)。结果:与慢性非萎缩性胃炎组比较,萎缩性胃窦炎组血清G-17水平明显降低,胃癌组血清G-17水平明显升高,萎缩性胃窦炎组及胃癌组血清PGⅠ及PGR均明显降低,具有统计学意义(P0.05)。与萎缩性胃窦炎组比较,胃癌组血清PGⅠ值明显降低,有统计学意义(P0.05),PGR降低,无统计学意义。结论:血清G-17、PGⅠ、PGR在萎缩性胃窦炎及胃癌中的表达具有特异性,可作为萎缩性胃窦炎和胃癌的筛查指标。     

胃蛋白酶原、胃泌素17和细胞毒素相关蛋白A检测在上消化道肿瘤中的价值

目的 探讨血清中胃蛋白酶原(pepsinogen,PG)I、PGII、胃蛋白酶原比值(pepsinogen ratio,PGR,PGI/PGII)和胃泌素17(gastrin-17,G-17)的水平以及幽门螺旋杆菌细胞毒素相关蛋白A(cytotoxin-associated protein,CagA)在萎缩性胃炎和胃癌筛查中的价值,为胃癌的早期防治提供科学依据。方法 选取就诊于武警边防部队总医院的186例胃肠道疾病患者和健康体检人群作为研究对象;依据胃镜和病理确诊的结果分为萎缩性胃炎组53例、早期胃癌组39例、进展期胃癌组46例和健康对照组(含轻度非萎缩性胃炎)48例。采集受检人群的空腹血,同时检测血清中PGI、PGII、G-17和CagA四项标志物,其中PGI、PGII、G-17采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法进行定量检测,CagA采用免疫印迹法进行定性检测。结果 在萎缩性胃炎和胃癌患者中,CagA的阳性率高于对照组(P<0.01),PGI的水平低于对照组(P<0.05);CagA阳性的患者其PGI的水平和PGR要低于CagA阴性的患者(P<0.05),而PGII和G-17的水平要高于CagA阴性的患者(P<0.05)。早期、进展期两组胃癌患者与对照组和萎缩性胃炎组相比,其PGII和G-17的水平均升高(P<0.05),PGI的水平和PGR降低(P<0.05),且进展期胃癌组PGI的水平要低于早期胃癌组(P<0.05)。在对照组和萎缩性胃炎组之间PGII、G-17的水平和PGR的差异无统计学意义(P>0.05)。结论 CagA感染与萎缩性胃炎和胃癌有相关性,在萎缩性胃炎和胃癌患者中PGI的水平降低;在胃癌患者中PGII和G-17的水平升高,PGR降低;PGI、PGII和G-17的水平变化与CagA感染有关。联合检测血清中PGI、PGII、G-17和CagA可对萎缩性胃炎和胃癌患者进行早期筛查。     

血清胃蛋白酶原、胃泌素-17及幽门螺杆菌检测在胃癌及癌前病变诊断中的价值研究

目的探讨血清胃蛋白酶原(PG)、胃泌素-17(G-17)及幽门螺杆菌(Hp)检测在胃癌及癌前病变诊断中的应用价值。方法选取该院2017年1月～2018年1月治疗的168例胃部疾病患者为研究对象,分为浅表性胃炎45例、胃溃疡40例、萎缩性胃炎30例、非典型增生28例、胃癌25例,同时选取38例健康体检者为对照组。采用荧光免疫层析法检测各组血清PGⅠ、PGⅡ、PGⅠ/PGⅡ(PGR)及G-17水平。~(13)C尿素呼气试验检测各组Hp并比较Hp感染阳性率。对各组及Hp阳性组、Hp阴性组血清PGⅠ、PGⅡ、PGR及G-17水平进行分析比较。结果与其余各组相比,胃癌组PGⅠ明显降低,溃疡组PGⅠ明显升高(P0.01)。胃癌组、溃疡组及非典型增生组PGⅡ明显高于对照组(P0.01)。胃癌组PGR明显低于其余各组,非典型增生组及萎缩性胃炎组PGR明显低于对照组、浅表性胃炎组及溃疡组(P0.01)。胃癌组G-17明显高于其余各组,萎缩性胃炎组G-17明显低于溃疡组、非典型增生组及胃癌组。溃疡组、萎缩性胃炎组、非典型增生组及胃癌组Hp阳性率明显高于对照组(P0.05)。与Hp阴性组相比,Hp阳性组PGⅠ及PGR明显降低,G-17明显升高(P0.01)。结论血清胃蛋白酶原、胃泌素-17及幽门螺杆菌检测在胃癌及癌前病变的诊断中具有重要价值。     

血清胃蛋白酶原、胃泌素17在胃癌筛查中的检测价值

目的:探讨胃蛋白酶原(PG)、胃泌素17(G-17)的血清学检测在胃癌筛查中的价值。方法:选取门诊及住院患者505例,通过酶联免疫吸附试验检测患者的血清PG I、PG II、G-17水平,计算PG I/PG II比值(PGR),并对患者进行胃镜、活检检查。根据血清PG I、PGR、G-17将受检者分为A[G-17(-)PG(-)]、B[G-17(+)PG(-)]、C[G-17(-)PG(+)]、D[G-17(+)PG(+)]组,根据胃镜检查将受检者分为浅表性胃炎组、萎缩性胃炎组和胃癌组(包括早期胃癌和进展期胃癌)。结合内镜活检和病理检查结果,对比分析受检者血清PG和G-17与胃癌的关系。结果:受检者中早期及进展期胃癌共17例(3.4%),浅表性胃炎112例(22.2%),萎缩性胃炎376例(74.5%),胃癌检出率为3.4%(17/505)。A、B、C、D四组胃癌检出率分别为1.0%(3/295)、4.8%(1/21)、2.6%(4/151)、23.7%(9/38);D组胃癌检出率明显高于A、B、C组,差异有统计学意义(P<0.05)。浅表性胃炎组、萎缩性胃炎组和胃癌组PGI水平比较差异无统计学意义(P>0.05);胃癌组PGR水平低于浅表性胃炎组,差异有统计学意义(P<0.05);萎缩性胃炎组G-17水平高于浅表性胃炎组,差异有统计学意义(P<0.05)。结论:血清PG、G-17可以作为胃癌筛查的指标,对于筛查阳性的患者行胃镜及病理检查,尤其是胃镜精查,既可明显提高胃癌的检出率,又可减少患者痛苦。     

血清胃蛋白酶原与胃溃疡及胃癌

目的 通过检测血清胃蛋白酶原I、血清胃蛋白酶原Ⅱ,探讨胃溃疡及胃癌患者血清胃蛋白酶原Ⅰ、血清胃蛋白酶原Ⅱ及血清胃蛋白酶原I/血清胃蛋白酶原Ⅱ比值的变化规律.方法 用免疫放射法测定血清PGⅠ及PGⅡ并计算PGI/PGⅡ的比值即PGR.结果 消化性溃疡患者血清PGI及PGⅡ升高.在溃疡组的分层研究中,血清PGI及PGR升高在活动组更为明显,而在愈合组变化无统计学意义.胃癌患者血清PGI降低、PGR降低.在胃癌组的分层研究中:早期胃癌患者PGI及PGR明显降低;进展期胃癌患者PGI及PGR亦降低,两者间无统计学差异.结论 测定血清PGI、PGⅡ水平及PGR值对胃溃疡及胃癌患者的鉴别诊断具有重要的参考意义.     

血清PG、G-17联合CA72-4和13C UBT在早期胃癌的诊断价值

目的 探讨血清胃蛋白酶原(PG)、胃泌素17(G-17)在胃癌的水平变化情况,在此基础之上联合糖类抗原72-4(CA72-4)以及13C尿素呼气试验(13C UBT)的检测在早期胃癌的诊断意义.方法 使用酶联免疫吸附试验(ELISA)检测健康人(对照组)、萎缩性胃炎组和胃癌组血清PGⅠ、PGⅡ、G-17的水平,先比较以上3种指标的变化情况;后根据PGⅠ和G-17将胃癌组分为A、B、C、D组后,对4组受检者,采用13C呼气试验检测幽门螺杆菌感染情况,利用肿瘤标记物检测结果统计CA72-4的水平情况.结果 PGⅠ血清水平和PG Ⅰ/PGⅡ比值在对照组、萎缩性胃炎组以及胃癌组中逐渐下降,差异有统计学意义(P＜0.05);G-17血清水平在对照组、萎缩性胃炎组以及胃癌组中逐步增加,3组之间水平差异有统计学意义(P＜0.05);A～D 4组间早期胃癌率比值中,B组的早期胃癌阳性率最高,差异有统计学意义(P＜0.05);4组中比较”C UBT和CA72-4水平在早期胃癌的阳性率的情况,B组13C阳性率和CA72-4水平高于ACD组,差异均有统计学意义(P＜0.05).并且B组CA72-4水平在早期胃癌和进展期胃癌差异上更加明显(P＜0.01).结论 血清PG Ⅰ降低和G-17水平升高联合CA72-4高水平和”C UBT阳性对于胃癌的诊断具有重要的预警价值.     

血清TSGF、HP抗体联合多肿瘤标志物对胃癌早期诊断的临床价值

目的探讨血清TSGF、HP抗体、CEA、CA724、CA242、CA19-9及胃蛋白酶原联合检测在胃癌早期诊断中的临床意义。方法采用回顾性分析方法,连续收集从2017年1月至2018年12月就诊于聊城市中医医院脾胃病科及肿瘤科的患者,符合纳入标准、排除标准的143例病例资料。其中经病理证实为胃癌的49例作为胃癌组,48例胃部萎缩性胃炎病变作为萎缩胃炎组,46例胃部非萎缩性胃炎病变作为非萎缩胃炎组,50例健康体检者为健康对照组。检测血清TSGF、HP抗体、CEA、CA242、CA19-9、CA724、PGI、PGⅡ及PGR水平,比较胃癌组、慢性非萎缩性胃炎组、慢性萎缩性胃炎组和健康对照组之间的差异。结果三组患者和健康对照组的血清TSGF、CEA、CA242、CA19-9、CA724、进行比较,慢性非萎缩性胃炎组、慢性萎缩性胃炎组、健康对照组与胃癌组进行比较有显著差异(P0.05);慢性非萎缩性胃炎组、慢性萎缩性胃炎组与健康对照组比较P0.05,无统计学意义。PGⅠ、PGⅡ、PGⅠ/PGⅡ(PGR)水平慢性萎缩性胃炎组、胃癌组与健康对照组比较有显著差异(P0.05);慢性非萎缩性胃炎组与健康对照组比较(P0.05),无统计学意义。Hp-IgG检测呈阳性的在健康对照组26例(52.0%)、慢性非萎缩性胃炎组29例(63%)、慢性萎缩性胃炎组32例(66.7%)、胃癌组36例(73.5%),Hp-IgG阳性率胃癌组慢性萎缩性胃炎组慢性非萎缩性胃炎组健康对照组。多肿瘤标志物联合检测在诊断胃癌早期的灵敏度高达85.9%,特异度为84.9%。结论血清TSGF、Hp抗体、CEA、CA19-9、CA72-4、CA242的水平与胃癌发展有关,在胃癌的早期诊断具有一定的价值,血清PGI、PGR水平降低对胃癌早期诊断具有重要的意义。联合检测有利于提高胃癌患者早期诊断的灵敏度和特异度。     

慢性萎缩性胃炎患者血清胃蛋白酶原的研究

目的:通过检测不同类型慢性胃病患者是否感染幽门螺杆菌(Hp)和血清胃蛋白酶原(PG)水平,探讨Hp感染的慢性胃病患者的血清PG水平变化,为慢性萎缩性胃炎及早期胃癌的诊断与治疗提供依据。方法:应用化学发光法对105例住院患者血清中PG水平进行了检测,观察不同疾病组患者PG水平变化;同时观察Hp感染相关慢性萎缩性胃炎、胃癌患者及非Hp感染萎缩性胃炎、胃癌患者血清中PG水平,判断Hp感染对PG水平变化的影响。结果:各组PGⅠ的比较中,除非萎缩性胃炎组,其他组与对照组均有统计学差异(P<0.01);各组PGⅡ的比较中,胃癌组与对照组存在统计学差异(P<0.01);各组PGⅠ/PGⅡ的比较中,除非萎缩性胃炎组,其他组与对照组均有统计学差异(P<0.01);除对照组的其他四组两两比较中均有统计学差异(P<0.01);萎缩性胃炎组和胃癌组Hp阳性与Hp阴性比较中PGⅠ、PGⅡ、PGⅠ/PGⅡ有显著差异(P<0.01)。结论:Hp慢性感染与慢性萎缩性胃炎、早期胃癌的发生发展密切相关,PGI和PGI/PGⅡ水平明显减低,这一结果对慢性萎缩性胃炎、早期胃癌的发现及诊断有重要的意义。     

胃蛋白酶原 胃泌素17及幽门螺杆菌检测在早期胃癌诊断中的应用价值

目的 探究胃蛋白酶原(PG)、胃泌素17(G-17)及幽门螺杆菌(Hp)检测在早期胃癌诊断中的应用价值。方法 回顾性分析2019年8月至2022年9月于江西省乐平市中医医院和江西省乐平市人民医院进行检查的90例胃部不适患者的临床资料,所有患者均经胃镜及病理检查明确诊断,依据诊断结果将患者分为早期胃癌组(38例)、萎缩性胃炎组(30例)及进展期胃癌组(22例),另选择30名均为同期于医院行健康体检的志愿者入对照组。对比各组检查指标[胃蛋白酶原Ⅰ(PGⅠ)、蛋白酶原Ⅱ(PGⅡ)、G-17、胃蛋白酶原比值(PGR)];对比各组Hp阳性检出率,以及不同性质Hp患者血清各指标水平。结果 萎缩性胃炎组、早期胃癌组及进展期胃癌组PGⅠ、PGR水平均低于对照组,PGⅡ水平均高于对照组,早期胃癌组及进展期胃癌组G-17水平均高于对照组,差异有统计学意义(F=20.346、18.472、16.345、19.326,P均<0.01);萎缩性胃炎组G-17水平与对照组相比,差异无统计学意义(t=1.921,P=0.060),且早期胃癌组与萎缩性胃炎组PGⅡ及PGR水平对比,差异无统计学意义(t=0.69...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.