CHEMOTACTIC ACTIVITY FOR POLYMORPHONUCLEAR AND MONONUCLEAR LEUKOCYTES IN RHEUMATOID SYNOVIAL FLUIDS

In order to why polymorphonuclear leukocytes (PMNs) are predominant and mononuclear leukocytes (MNLs) are few in rheumatoid synovial fluids, chemotactic factor(s) for PMNs and MNLs were studied in the synovial fluids of rheumatoid arthritis (RA-SF) and osteoarthritis (OA-SF) using both Boyden's and agarose methods. The RA-SF showed strong chemotactic activity for human peripheral blood PMNs compared with non-rheumatoid OA-SF. The chemotactic activity for PMNs was well correlated with the number of PMNs in RA-SF, suggesting that it was a natural mediator for PMN emigration into rheumatoid joint cavity. The major chemotactic factor for PMN in RA-SF was of apparent molecular weight of 14,000 and its activity was suppressed to less than 10 percent by anti-C5a antibody, but it failed to show any anaphylatoxin activity which was an attribute of C5a. It was, therefore, suggested to be C5a-like molecule but not C5a itself. The possibility that the factor may be a C5a des-Arg was discussed. On the contrary, the chemotactic activity for MNLs was not found neither in RA-SF nor OA-SF. These findings may explain the fact that PMNs are predominant in rheumatoid synovial fluids.     

再生障碍性贫血患儿免疫状况及其外周血IL-2活性水平的研究

本文对13例小儿再障的细胞免疫状况及T淋巴细胞亚群和外周血淋巴细胞诱生的白细胞介素-2(IL-2)活力进行测定。其中3例作免疫治疗前后的动态比较。结果显示患儿总T 细胞(CD_3)及T辅助细胞(CD_4)均低于正常儿,而T抑制细胞(CD_8)则高于正常儿,使CD_4/CD_8的比值明显低于正常儿(0.91±0.46:1,正常儿1.67±0.24:1,P<0.01)。而IL-2活性在患儿白细胞中高于正常高限(529.76±±36.84比316.31±53.99,P<0.01),说明患儿有免疫紊乱状态。应用免疫抑制治疗(ATG,环孢素A等),可纠正这种不平衡状态,临床也有良好的效应。     

PRODUCTION OF PYROGEN BY POLYMORPHONUCLEAR LEUKOCYTES DURING THE COURSE OF CASEIN-INDUCED PERITONITIS IN RABBITS

Rabbits with casein-induced peritonitis were febrile only during the early stage (1.5 to 12 hours) of inflammation. At that stage, peritoneal exudate cells (PEC) had preformed endogenous pyrogen (EP), while blood leukocytes and leukocytes obtained at the later stage of the inflammatory process did not. Early PEC consisted of 99% polymorphonuclear leukocytes (PMN). As early PEC and highly purified PMN (almost 100% pure) released almost identical amounts of EP, we concluded that PMN were the EP-producing cells in the early PEC. EP from PMN of rabbits was composed of three factors with similar molecular weight (8,000 to 18,000) but different pI values (pI 7.2, 5.4, and 4.5). EP in the three fractions could not be separated from thymocyte comitogenic factor (TMF) in terms of m.w. and pI values. About 60% of EP activity and 92% of TMF activity in the culture supernatant were present in the pi 7.2 fraction. The pi 7.2 factor was weaker in EP activity and stronger in TMF activity than the other acidic fractions (pI 5.4 and 4.5). After high purification, 42 ng of the pI 7.2 factor had one unit of EP activity: one unit of TMF activity was present in 126 pg of highly purified pi 7.2 factor.     

ENHANCEMENT OF PHYTOHEMAGGLUTININ-INDUCED DNA SYNTHESIS OF MOUSE THYMOCYTES AND T-LYMPHOCYTES BY A NEUTRAL PROTEASE OF POLYMORPHONUCLEAR LEUKOCYTES

Mouse thymocytes were poorly triggered in vitro with phytohemagglutinin (PHA) to induce DNA synthesis and the response was markedly enhanced by culture supernatant (SUP) of polymorphonuclear leukocytes (PMN). The PMN factor was nondialysable, heat-labile and precipitated with 65% ammonium sulphate. Its molecular weight was approximately 19,000 on sephadex G-75. The sephadex fraction had a proteolytic activity on 3H-acetyl hemoglobin at neutral pH. The protease seemed to be a chymotrypsin-like enzyme on the basis of inhibition profile using various protease inhibitors. The thymocyte-helping activity in the protease fraction was absorbed by affinity columns of protease inhibitors. The PMN-protease also enhanced the DNA synthesis by antigen-stimulated lymph node cells and by PHA-stimulated T-lymphocytes, but not by LPS-stimulated B-lymphocytes. Only the lymphocytes already been stimulated with antigen or mitogen received preferentially the helping action by PMN-protease. The helping activity was effectively absorbed by PHA-stimulated thymocytes but not by nonstimulated thymocytes. These evidences seems to suggest that acceptor sites for the protease, newly developed on lymphocyte surface after stimulation, may play an important role in the enhanced DNA synthetic response. ACTA PATH. JAP. 27: 857-868, 1977.     

ELISA法定量检测兔中性粒细胞CD18的表达

应用其自建的定量检测细胞粘附分子（ｃｅｌｌａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅｓ，ＣＡＭｓ）表达的细胞酶联免疫吸附测定法（ｃｅｌｌ－ＥＬＩＳＡ），将兔中性粒细胞（ＰＭＮ）用０．５％甲醛－ＨＢＳＳ固定于９６孔板，通过检测ＰＭＮ上结合物的酶活性和ＰＭＮ的蛋白量，以每分钟内每微克ＰＭＮ上的酶活性（ＯＤ／ｍｉｎ／μｇｐｒｏｔｅｉｎ）相对表示细胞粘附分子的表达，并用此法分别检测了静息状态和ＴＮＦ激活的兔ＰＭＮＣＤ１８的表达。     

ARHINENCEPHALY WITH NORMAL KARYOTYPE ASSOCIATED WITH HYPOCALCEMIC CONVULSION

The patient was a male infant who was born at the 39th week of gestation. He had cleft lips and palate, low set ears, myogenic torticollis in the left neck, clubhand in both 5th fingers, dysplasia of the penis, and cryptorchism. Although he had convulsion due to hypocalcemia 9 days after birth, this improved 25 days after birth by the administration of a calcium agent. Five months after birth, cleft lip plastics were performed. Six months after birth, an apnea attack occurred and cyanosis was observed during examination of the optic fundi. Two days later, he died. Chromosomal analyses has revealed a normal chromosome karyotype 17 days and again 4th months after birth. Autopsy findings showed the brain to be normally separated into two cerebral hemispheres but lacking in olfactory bulbi and tracts. Also observed were an atrio-septal cardiac defect, Meckel's diverticulum in the lower small intestine, and enlarged renal pelves. Retinal and choroid defects were seen at the sites where giant papillae were found in examination of the optic fundi.     

IgM-MONOCLONAL GAMMOPATHY ASSOCIATED WITH MALIGNANT LYMPHOMA WITH RECURRENT PLEURAL EFFUSION

A case of malignant lymphoma (plasmacytold lymphocytic type) with IgM-monoclonal gammopathy is presented. The main site of the lesion was in the lung. The sole clinical manifestation was pleuropulmonary involvement with massive pleural effusion. A clue to the diagnosis was given by cytologlcal and immunocytological examination of pleural aspirates. Subsequent immunological survey of serum protein and a bronchial biopsy confirmed the diagnosis.     

二硫化碳接触者的脑干诱发电位分析

本文对22例接触二硫化碳10年以上的职工进行脑干诱发电位的检查,并以50例正常人进行对照,结果证实:多数患者有不同程度的异常,主要表现为Ⅲ-Ⅴ/Ⅰ-Ⅲ>1,Ⅴ/Ⅰ<0.5,与正常人组有显著差异(P<0.01)