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T. Skottman H. Piiparinen H. Hyytiäinen V. Myllys M. Skurnik S. Nikkari 《European journal of clinical microbiology & infectious diseases》2007,26(3):207-211
This report describes the development of in-house real-time PCR assays using minor groove binding probes for simultaneous
detection of the Bacillus anthracis pag and cap genes, the Francisella tularensis 23 KDa gene, as well as the Yersinia pestis pla gene. The sensitivities of these assays were at least 1 fg, except for the assay targeting the Bacillus anthracis cap gene, which showed a sensitivity of 10 fg when total DNA was used as a template in a serial dilution. The clinical value
of the Bacillus anthracis- and Francisella tularensis-specific assays was demonstrated by successful amplification of DNA from cases of cow anthrax and hare tularemia, respectively.
No cross-reactivity between these species-specific assays or with 39 other bacterial species was noted. These assays may provide
a rapid tool for the simultaneous detection and identification of the three category A bacterial species listed as biological
threats by the Centers for Disease Control and Prevention. 相似文献
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R. G. Vancini A. Pereira-Neves R. Borojevic M. Benchimol 《European journal of clinical microbiology & infectious diseases》2008,27(4):259-267
The parasite Trichomonas vaginalis causes one of the most common non-viral sexually transmitted infections in humans. Mycoplasmas are frequently found with
trichomonads but the consequences of this association are not yet known. In the present study, the effects of T. vaginalis harboring M. hominis on human vaginal epithelial cells and on MDCK cells are described. The results were analyzed by light, scanning and transmission
electron microscopy, as well as using cell viability assays. There was an increase in the cytopathic effects on the epithelial
cells infected with T. vaginalis associated with M. hominis compared to T. vaginalis alone. The epithelial cells exhibited an increase in the intercellular spaces, a lesser viability, and increased destruction
provoked by the infected T. vaginalis. In addition, the trichomonads presented a higher amoeboid transformation rate and an intense phagocytic activity, characteristics
of higher virulence behavior. 相似文献
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Aneisha M. Collins Judith K. Brown Malik Mujaddad Rehman Marcia E. Roye 《Archives of virology》2009,154(11):1859-1860
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I. Chmelnitsky S. Navon-Venezia A. Leavit E. Somekh G. Regev-Yochai M. Chowers P. Shitrit Y. Carmeli 《European journal of clinical microbiology & infectious diseases》2008,27(5):385-390
Molecular characterization of methicillin-resistant Staphylococcus aureus isolates from three hospitals in Israel was the aim of the study presented here. We identified 11 distinct genetic clones
by pulsed-field gel electrophoresis. Molecular typing identified four different SCCmec types—I, II, IV, and V−and nine spa types. Spa type t002 was the most common.
An erratum to this article can be found at 相似文献
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Zbigniew Zwierzykowski Elżbieta Zwierzykowska Magdalena Taciak Neil Jones Arkadiusz Kosmala Paweł Krajewski 《Chromosome research》2008,16(4):575-585
Genomic in situ hybridization (GISH) was used to make a detailed study of chromosome pairing at metaphase I (MI) of meiosis in six F(1) hybrid plants of the allotetraploid Festuca pratensis x Lolium perenne (2n = 4x = 28; genomic constitution FpFpLpLp). The mean chromosome configurations for all hybrids analysed were 1.13 univalents + 11.51 bivalents + 0.32 trivalents + 0.72 quadrivalents, and the mean chiasma frequency was 21.96 per cell. GISH showed that pairing was predominantly intragenomic, with mean numbers of L. perenne (Lp/Lp) and F. pratensis (Fp/Fp) bivalents being virtually equal at 5.41 and 5.48 per cell, respectively. Intergenomic pairing between Lolium and Festuca chromosomes was observed in 33.3% of Lp/Fp bivalents (0.62 per cell), in 79.7% of trivalents - Lp/Lp/Fp and Lp/Fp/Fp (0.25 per cell), and in 98.4% of quadrivalents - Lp/Lp/Fp/Fp and Lp/Lp/Lp/Fp (0.71 per cell). About 4.0% of the total chromosome complement analysed remained as univalents, an average 0.68 Lp and 0.45 Fp univalents per cell. It is evident that in these hybrids there is opportunity for recombination to take place between the two component genomes, albeit at a low level, and this is discussed in the context of compromising the stability of Festulolium hybrid cultivars and accounting for the drift in the balance of the genomes over generations. We speculate that genotypic differences between hybrids could permit selection for pairing control, and that preferences for homologous versus homoeologous centromeres in their spindle attachments and movement to the poles at anaphase I could form the basis of a mechanism underlying genome drift. 相似文献
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M. E. Falagas E. A. Karveli I. Kelesidis T. Kelesidis 《European journal of clinical microbiology & infectious diseases》2007,26(12):857-868
Acinetobacter infections have been attracting increasing attention during recent years because they have become common in hospitalized patients, especially in the intensive care unit (ICU) setting. However, the available literature suggests that the pathogen has another fearful potential; it can cause community-acquired infections. We searched PubMed and the reference lists of the initially identified articles and identified six case series regarding a total of 80 patients with community-acquired Acinetobacter baumannii infections; from these, 51 had pneumonia and 29 had bacteremia. Of these 80 patients, 45 (56%) died of the infection. In addition, we identified 26 case reports regarding 43 patients with community-acquired Acinetobacter infections; from these, 38 had pneumonia, two had meningitis, one had soft-tissue infection, one had ocular infection, and one had native valve endocarditis. Comorbidity was commonly present in patients reported in the case series as well as the case reports, mainly, chronic obstructive pulmonary disease, renal disease, and diabetes mellitus; heavy smoking and excess alcohol consumption were also common. Most of the studies originated from China, Taiwan, and tropical Australia. We also identified 12 retrospective or prospective studies (seven from the Far East, two from Oceania, one from N. Guinea, one from Palestine, and one from USA/Canada) that reported the frequency of community-acquired Acinetobacter infections; the range of isolation of Acinetobacter from patients with community-acquired pneumonia in these studies was 1.3%-25.9%. In conclusion, most community-acquired Acinetobacter infections have been reported from countries with tropical or subtropical climate, and mainly affect patients with some form of comorbidity or are associated with heavy smoking and excess alcohol consumption. 相似文献
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Ahmad Oryan Davood Mehrabani Seyed Mohammad Owji Mohammad-Hossein Motazedian Qasem Asgari 《Comparative clinical pathology》2007,16(4):275-279
Cutaneous leishmaniasis (CL) is a zoonotic disease transmitted between rodents and canines, mainly by Phlebotomus sand flies and man. In southern Iran, the incidence of this protozoan disease has doubled over the last decade. The present
study deals with histopathological features of CL in Tatera indica and Gerbillus spp. that participate in the epidemiology of CL in southern Iran. Thirty-two trapped rodents were evaluated for any Leishmania infection using enzyme electrophoresis and polymerase chain reaction and were concomitantly studied for any histopathological
changes. Histopathological studies showed that bone marrow was the tissue of choice for light and electron microscopic study
of Leishmania, demonstrating the macrophages infected with the amastigote form of the parasite. This is the first report of the histopathological
detection of L. major in naturally infected T. indica and Gerbillus spp in the Larestan region. 相似文献
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Fabricia S Predes Ana LTG Ruiz João E Carvalho Mary A Foglio Heidi Dolder 《BMC complementary and alternative medicine》2011,11(1):25
Background
Arctium lappa, known as burdock, is widely used in popular medicine for hypertension, gout, hepatitis and other inflammatory disorders. Pharmacological studies indicated that burdock roots have hepatoprotective, anti-inflammatory, free radical scavenging and antiproliferative activities. The aim of this study was to evaluate total phenolic content, radical scavenging activity by DPPH and in vitro antiproliferative activity of different A. lappa root extracts. 相似文献13.
M. P. Ryan J. T. Pembroke C. C. Adley 《European journal of clinical microbiology & infectious diseases》2011,30(10):1245-1247
Differentiation of the growing nosocomial infectious threats, Ralstonia pickettii and Ralstonia insidiosa, based on nitrate reduction, desferrioxamine susceptibility, arabinose, N-acetyl-glucosamine and phenylacetate assimilation
is described. These tests can be used for preliminary identification of Ralstonia pickettii and Ralstonia insidiosa resulting in more accurate identification of these species. 相似文献
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N. V. Fomenko O. V. Stronin M. N. Khasnatinov G. A. Danchinova J. Bataa N. A. Gol’tsova 《Molecular Genetics, Microbiology and Virology》2009,24(4):183-188
In this work, identification and analyses of 48 full-length sequences of the ospA gene isolates of Borrelia garinii and Borrelia afzelii from Western Siberia and Mongolia has been made. It was shown that B. garinii isolates was of its high genetic heterogeneity of the ospA gene. Four genetic groups of the ospA gene from the Ixodes persulcatus tick collected in of Western Siberia and Mongolia were defined. The basic differences in the genetic variants of the ospA gene considered are seen in regions which code for antibody determinants of thhe OspA protein. 相似文献
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A. Rymaszewska 《European journal of clinical microbiology & infectious diseases》2008,27(11):1025-1036
Anaplasma phagocytophilum is an obligate intracellular bacterial parasite in human and animal granulocytes. In Europe, A. phagocytophilum is transmitted by Ixodes ticks; Ixodes ricinus is the vector of the parasite in Poland. In terms of epidemiology, the identification of pathogens in ticks increasingly
relies on molecular techniques. Polymerase chain reaction (PCR) with species-specific primers is a tool that allows the quick
and accurate detection of pathogens in ticks, humans, or animals. DNA was extracted from the blood of Capreolus capreolus and Cervus elaphus, and amplified using the primers HS1/HS6 (external) and HS43/HSVR (internal). For sequencing, six samples from roe deer and
two samples from red deer were selected, and the resulting sequences were submitted to GenBank (accession numbers DQ779568,
DQ779567, EU157919, EU157920, EU157921, EU157922). These nucleotide sequences were compared with each other and five variants
were distinguished in roe deer and one in red deer. A comparison of the sequences of the author’s database revealed 45 polymorphic
sites of substitution character (76% transitions and 24% transversions). The homology tree revealed two groups, one with sequences
only from roe deer, while the second with sequences isolated mainly from red deer, livestock animals, and humans. These strains
of A. phagocytophilum are also present in Poland. 相似文献
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N. U. Matsenko V. S. Rijikova S. P. Kovalenko 《Bulletin of experimental biology and medicine》2008,145(2):240-244
We compared two technologies of real-time PCR (with the use of fluorescent SYBR Green I dye and specific TaqMan probe) for quantification of the dose of her2 gene in breast tumors. The maximum increase in the gene dose in TaqMan and SYBR Green I analyses was 10-and 5-fold, respectively. In was found that TaqMan and SYBR Green I technologies allow detection of the matrix in amounts corresponding to 1–100 and 2.5–40.0 ng genomic DNA, respectively. Tenfold
increase in the gene dose leads to incorrect evaluation of multiplication ratio in the SYBR Green I analysis. These results suggest that TaqMan technology is more preferable for correct evaluation of her2 gene dose.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 145, No. 2, pp. 201–205, February, 2008 相似文献
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G. A. Eroshenko N. A. Vidaeva G. N. Odinokov L. M. Kukleva Ya. M. Krasnov N. P. Guseva V. V. Kutyrev 《Molecular Genetics, Microbiology and Virology》2009,24(3):138-143
Structural and functional analyses of the araC gene, which is involved in regulation of expression of diagnostically important characteristics, such as arabinose fermentation
in the plague microbe strain, were carried out. It was established that the reason for the lack of arabinose fermentation
in the altaica and hissarica subspecies and strains of talassica groups is the presence of the insertion of a single nucleotide in the araC gene at position 773 bp. The strains of the basic, caucasia, and ulegeica subspecies do not contain this mutation in the araC gene, which correlates to their ability to ferment arabinose. 相似文献
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The dried flowers and buds of Sophora japonica are used as a medicinal herb in China, Japan and Korea to treat bleeding hemorrhoids and hematemesis. This article presents
an overview of the effects of Sophora japonica on cerebral infarction based on literature searched from Medline, PubMed, Cochrane Library and the China National Knowledge
Infrastructure (CNKI). Sophora japonica contains both anti-hemorrhagic and anti-hemostatic substances. Sophora japonica reduces cerebral infarction partly as a result of its anti-oxidative and anti-inflammatory activities. Previous studies found
that Sophora japonica reduced the size of cerebral infarction and neurological deficits and reduced microglial activation, interleukin-1β release
and number of apoptotic cells in ischemia-reperfusion injured Sprague-Dawley rats. Further study is required to determine
the relationship between Sophora japonica-mediated reduction in cerebral infarction size and the effects of Sophora japonica on platelet aggregation and cardiovascular function. 相似文献
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M. L. H. Cuijpers F. J. Vos C. P. Bleeker-Rovers P. F. M. Krabbe P. Pickkers A. P. J. van Dijk G. J. A. Wanten P. D. Sturm W. J. G. Oyen B. J. Kullberg 《European journal of clinical microbiology & infectious diseases》2007,26(2):105-113
Complicating infectious foci resulting from haematogenous or local spread of microorganisms are observed frequently in patients
with Staphylococcus aureus bacteraemia (SAB) or Streptococcus species bacteraemia (SSB). The aim of this study was to compare the epidemiology of complicating infectious foci during SAB
and SSB in a university hospital in The Netherlands. The charts of all adult patients diagnosed with SAB or SSB (except for
Streptococcus pneumoniae bacteraemia) from July 2002 until December 2004 were reviewed retrospectively. Overall, 180 immunocompetent patients were
identified, 127 with SAB and 53 with SSB. The percentage of patients with complicating infectious foci (39% of SAB patients,
25% of SSB patients) did not differ significantly between the groups. Endocarditis and cerebral involvement, however, were
significantly more common in the SSB group. Of all complicating infectious foci, 32% lacked guiding signs or symptoms and
10% were detected only at autopsy. Factors associated with the development of complicating infectious foci were a delay in
treatment for more than 48 h after the onset of symptoms, community acquisition, persistently positive blood cultures, congenital
heart disease, and the presence of foreign bodies or prosthetic valves. Infection-related mortality was 18% in SAB patients
and 11% in SSB patients and was significantly higher in patients with complicating infectious foci (29 vs. 9%). In conclusion,
complicating infectious foci develop in approximately one-third of all patients with SAB and SSB. An active approach that
entails searching for the complicating infectious foci is warranted in these patients, because only two-thirds of complicated
infectious foci have guiding symptoms or signs, and infection-related mortality is significantly increased in patients with
complicating infectious foci compared to patients without these infections.
Authors M.L.H. Cuijpers and F.J. Vos contributed equally to this work. 相似文献
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Yvan?L’Homme Rapha?l?Sansregret étienne?Plante-Fortier Anne-Marie?Lamontagne Geneviève?Lacroix Mourad?Ouardani Jonathan?Deschamps Geneviève?Simard Carole?Simard 《Archives of virology》2009,154(4):581-593
Noroviruses and sapoviruses are members of the family Caliciviridae and emerging enteric pathogens of humans and animals.
Since their discovery and characterization in swine, relatively few strains have been described in detail. In order to investigate
their genetic diversity, a total of 266 fecal samples collected in the province of Quebec, Canada, between 2005 and 2007 were
screened for the presence of caliciviruses by RT-PCR using broadly reactive primers. Genetically heterogeneous caliciviruses
were detected on the majority of farms. Typical noroviruses related to known swine genotypes were present on 20% of the farms.
Sapoviruses were detected on 75% of the farms and were the most heterogeneous group. Further characterization of selected
strains in their 3′ end parts was carried out for their classification and unveiled possibly new clusters of sapoviruses.
No human-like noroviruses or sapoviruses were detected in the present study.
GenBank accession numbers of all sequences described in this study are indicated in the figure legends. 相似文献