免疫荧光法在检测分泌物淋病奈瑟氏球菌、沙眼衣原体中的应用价值

目的:探讨免疫荧光法在检测分泌物淋病奈瑟氏球菌、沙眼衣原体中的价值。方法:选取2019年3月?2019年8月在本院妇产科,男科,皮肤性病科就诊的疑似泌尿生殖道感染的患者600名为本次研究对象。样本取尿道分泌物或宫颈口分泌物送检。分别采用常规PCR法、免疫荧光法、培养法进行淋病奈瑟氏球菌、沙眼衣原体的检测。以培养法作为金标准,统计并比较免疫荧光法、常规PCR法对淋病奈瑟氏球菌、沙眼衣原体检测结果的诊断效能。结果:免疫荧光法对淋病奈瑟氏球菌、沙眼衣原体检测的准确度、敏感度、特异性、阳性预测值、阴性预测值均明显高于常规PCR法,差异有统计学意义(P<0.05)。免疫荧光法对淋病奈瑟氏球菌、沙眼衣原体检测的曲线下面积均明显高于常规PCR法,经比较差异有统计学意义(P<0.05)。结论:免疫荧光法检测淋病奈瑟氏球菌、沙眼衣原体快速、准确、灵敏,临床应用价值优于常规PCR法。     

卵巢切除和衰老对基底前脑的胆碱能神经元的影响

目的:本实验通过观察卵巢切除和衰老对基底前脑的内侧隔核和斜角带水平肢的乙酰胆碱转移酶胆碱能神经元变化,来探讨卵巢切除和衰老对基底前脑的胆碱能神经元胞体和突起的影响。方法:通过利用乙酰胆碱转移酶免疫组织化学的方法观察正常成年雌性组、切除卵巢组和老年组的动物胆碱能神经元形态和突起的变化。结果:切除卵巢组的动物的胆碱能神经元的细胞数(237±8),细胞大小(100.86±37.01)μm2,突起长度(100.86±37.01)与正常成年雌性组之间有明显的差异性(t=4.82～33.08,P<0.05)。正常雌性组和老年组的乙酰胆碱转移酶的细胞数(320±11,185±12;220±14,164±11),细胞大小(172±63,200±69;125±38,129±44),突出数目(1.17±0.45,1.64±0.23;0.76±0.45,1.17±0.42)有明显的差异性(t=9.11～31.02,P<0.05)。结论:切除卵巢和衰老能够引起基底前脑的胆碱能神经元胞体大小、数目及其突起数目的明显减少,这有可能解释衰老或神经退行性疾病导致的认知功能障碍。     

The management of septic abortion in an intensive care unit

The value of ICU treatment in the management of septic abortion has been assessed. Of patients subjected to hysterectomy, the mortality is now 20%, as opposed to 37% prior to the advent of ICU. It would therefore appear that these patients can benefit from intensive nursing and medical attention.     

The placebo effect of transcutaneous electrical stimulation.

The placebo effect of transcutaneous electrical stimulation was studied in 93 patients in a double-blind cross-over trial using a genuine stimulator and a placebo machine. Placebo analgesic effect occurred in 32% of trials, as compared with 48% for actual stimulation. The placebo effect of the transcutaneous electrical stimulator is similar to the placebo effect that is noted in other double-blind studies in which medications are used.