褪黑素对梗阻性黄疸大鼠肝损伤保护作用的实验研究

目的探讨氧化性损伤在大鼠梗阻性黄疸肝功能损害发生中的作用以及褪黑素对其的保护作用。方法成年雄性SD大鼠64只,采用完全随机化法随机分为正常对照组（CN组,n=16）、假手术组（SO组,n=16）、胆总管结扎组（BDL组,n=16）和胆总管结扎＋褪黑素治疗组（BDL＋MT组,n=16）。应用胆总管结扎法建立梗阻性黄疸模型,褪黑素治疗组大鼠手术前1 d至手术后7 d连续腹腔注射褪黑素0.5 mg（/kg.d）,每日10∶00给药。分别于手术后第4 d和第8 d两个时间点采集标本,检测血浆中总胆红素（TBIL）、丙氨酸转氨酶（ALT）、门冬氨酸转氨酶（AST）、碱性磷酸酶（AKP）及γ-谷氨酰转肽酶（GGT）水平变化,比色法测定肝组织匀浆中丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）含量或活力变化,采用TUNEL法检测肝组织细胞凋亡,并计算肝细胞凋亡指数（AI）。结果与CN组和SO组比较,BDL组大鼠血浆TBIL、ALT、AST、AKP、GGT水平和肝组织MDA含量明显升高（P〈0.05,P〈0.01）,SOD、CAT、GSH-Px活力或GSH含量显著降低（P〈0.01）,AI增加（P〈0.01）;褪黑素治疗可使血浆TBIL、ALT、AST、AKP、GGT和肝组织MDA含量显著降低,SOD、CAT、GSH-Px活力或GSH含量明显升高（P〈0.01）,AI减少（P〈0.01）。BDL组肝组织MDA含量与血浆TBIL、ALT、AKP、AST、GGT水平均呈显著正相关（P〈0.01）,GSH、SOD、CAT、GSH-Px与血浆TBIL、ALT、AKP、ALT、AST水平分别均呈显著负相关（P〈0.01）;BDL组肝组织MDA含量的变化与AI呈正相关（P〈0.01）,而GSH含量及SOD、CAT、GSH-Px活力分别与AI呈负相关（P〈0.01）。结论大鼠梗阻性黄疸时,肝组织自由基大量产生介导的氧化性损伤及其细胞凋亡,参与了肝功能损害的发生、发展。褪黑素对大鼠梗阻性黄疸肝功能损害有一定程度的保护作用,其机制可能与其拮抗肝组织过氧化和细胞凋亡有关。     

大黄酸对单侧输尿管梗阻大鼠肾脏的抗氧化保护作用

目的观察大黄酸（RH）对单侧输尿管梗阻（UUO）大鼠肾间质损伤的抗氧化保护作用。方法将30只雄性SD大鼠分成假手术组（Sham组）6只;UUO模型组（UUO组）和RH干预组（UUO＋RH组）各12只。除Sham组外,UUO组和UUO＋RH组分别在第3、7天测定大鼠左肾皮质匀浆中脂质过氧化物标志物丙二醛（MDA）及抗氧化酶过氧化氢酶（CAT）和超氧化物歧化酶（SOD）含量。结果UUO组较Sham组肾脏病理改变加重、肾组织MDA含量升高（P〈0.05）、SOD和CAT含量下降（P〈0.05）;UUO＋RH组较UUO组肾间质纤维化程度减轻、。肾组织MDA含量降低（P〈0.01）、SOD和CAT含量升高（P〈0.01）。结论RH能减少单侧输尿管梗阻侧肾皮质脂质过氧化物的产生,同时增加抗氧化酶的含量,通过改善UUO大鼠肾脏氧化应激来发挥肾脏保护作用。     

长期大量饮酒对大鼠阴茎组织结构和功能的影响

目的探讨长期大量饮洒对大鼠阴茎组织结构和功能的影响。方法将30只雄性大鼠随机分为对照组，20％酒精剂量组和40％酒精剂量组。分别以生理盐水，20％和40％食用酒精各2ml给大鼠灌胃，每日1次。3个月后，观察各组大鼠的性行为和药物诱发阴茎勃起的情况；用酶放大物理发光仪测定各组大鼠血清睾酮的含量；对阴茎平滑肌三色改良法染色，并利用图象分析管理系统，分别计算各组大鼠阴茎平滑肌的目标总面积和面密度。结果与对只照组相比，20％酒精剂量组大鼠性行为受到抑制（P〈0．05），大鼠血清睾酮含量降低（P〈0．05）；40％酒精剂量组人鼠性行为，药物诱发阴茎勃起实验均受到抑制（P〈0．05），血清睾酮含量和平滑肌细胞数量减少。结论大鼠长期大量饮洒后性功能障碍，其机制与血浆睾酮含量降低，阴茎海绵体平滑肌成分减少有关。     

细胞色素P4502E1和氧化应激与大鼠非酒精脂肪性肝炎的关系

目的观察大鼠非酒精脂肪性肝炎（nonalcoholic steatohepattiis,NASH）组织病理形态学改变,探讨其与细胞色素P4502E1的表达和氧化应激的关系。方法用高脂饮食制备非酒精脂肪性肝人鼠模型,正常组普通饲料喂养,12W末处死各组大鼠,全自动生化仪测定血清ALT、AST、血糖、胰岛素,光镜下观察肝组织病理形态学改变,测定肝组织CYP450及同工酶CYP2E1含量,TBA法和黄嘌呤氧化酶法测定肝组织MDA含量和SOD活性。结果与对照组比较,模型组大鼠血清ALT、AST、血糖、胰岛素显著升高（P〈0．05）,肝组织MDA及CYP2E1含量显著增高（P〈0．05）,SOD活性显著降低（P〈0．05）,肝脏的脂肪变性程度和炎症活动度均显著增高（P〈0．05）。结论成功复制NASH大鼠模型,CYP2E1与非酒精性脂肪性肝炎有关,MDA和SOD在NASH脂质过氧化反应中发挥重要作用。     

亚硒酸钠和维生素E对被动吸烟小鼠抗氧化能力和精子的影响

目的研究亚硒酸钠和维生素E对被动吸烟小鼠睾丸抗氧化能力及附睾精子的影响。方法小鼠置于自制被动吸烟箱中，每日吸烟两次，用药组小鼠分别给予亚硒酸钠、维生素E、亚硒酸钠和维生素E灌胃，连续35d。睾丸组织匀浆测超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）、硒含量。附睾精子观察密度、活动率、前向运动、快速前向运动精子百分比和畸形精子百分比。结果各用药组SOD、GSH-Px、硒含量和精子密度、活动率、前向运动精子、快速前向运动精子百分比均有不同程度的升高（P〈0．05，P〈0．01），MDA含量、畸形精子百分比降低（P〈0.05，P〈0．01）。结论亚硒酸钠、维生素E可对抗被动吸烟引起的小鼠睾丸组织氧化损伤，起到保护精子的作用。     

参芎注射液对肾缺血再灌注损伤大鼠的保护作用

目的观察参芎注射液对肾缺血再灌注损伤大鼠肾组织核因子-κB（NF-κB）、肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性的影响,探讨其肾保护作用机制。方法将24只SD大鼠随机分为假手术对照组、缺血再灌注组、参芎预处理组,每组8只。免疫组织化学法检测各组大鼠肾组织NF-κB蛋白表达,酶联免疫吸附法检测肾组织TNF-α含量,用MDA和SOD试剂盒分别检测肾组织MDA含量和SOD活性。结果①与假手术对照组相比,缺血再灌注组大鼠肾组织NF-κB蛋白表达、TNF-α和MDA含量明显升高,差异均有统计学意义（P〈0．01）;而SOD的活性明显降低,差异有统计学意义（P〈0．01）。②与缺血再灌注组相比,参芎预处理组大鼠肾组织NF-κB蛋白表达、TNF-α和MDA含量降低,差异均有统计学意义（P〈0．05或P〈0．01）;而SOD的活性明显升高,差异有统计学意义（P〈0．01）。结论参芎注射液对肾缺血再灌注损伤有一定的保护作用,其机制可能与抗自由基氧化损伤以及抑制炎性细胞因子NF-κB和TNF-α的表达有关。     

阿魏酸对UVB导致人角质形成细胞氧化损伤的保护作用研究

目的：探讨阿魏酸是否能对中波紫外线（UVB）诱导的人角质形成细胞（HaCaT细胞）氧化损伤和凋亡起保护作用以及其可能的作用机制。方法：将HaCaT细胞分成空白组、阿魏酸纽、UVB照射组、UVB照射＋阿魏酸纽。采用四甲基偶氮唑蓝（MTT）比色法检测细胞活性,筛选最佳药物浓度;检测细胞上清液中的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）和过氧化氢酶（CAT）活性以及丙二醛（MDA）含量变化。结果：UVB照射组的SOD、CAT、GSH-Px明显下降,MDA相应上升。阿魏酸处理的细胞UVB照射后其活性以及上清液中的SOD、GSH-Px、CAT活性相对于未加药UVB照射纽明显增加,MI）A相应下降。结论：阿魏酸可以减少UVB诱导HaCaT细胞氧化损伤,其机制可能与增强抗氧化成分活性和减少氧自由基有关。     

罗格列酮对糖尿病大鼠肾脏保护作用机制的探讨

目的 探讨罗格列酮对糖尿病大鼠肾脏的保护作用机制。方法 将大鼠分为正常组、糖尿病组、小剂量罗格列酮组、大剂量罗格列酮组。药物干预4周后，检测各组大鼠的血糖（BS）、三酰甘油（TG）、胆固醇（TC）、肌酐（Scr）水平及尿白蛋白定量（24h）。随后处死大鼠，测定肾组织中丙二醛（MDA）含量、总抗氧化能力（T-AOC）及铜锌超氧化物歧化酶（Cu-ZnSOD）、谷胱甘肽过氧化酶（GSH-Px）、核转录因子-κB（NF-κB）的活性。同时留取。肾脏组织作PAS染色行病理检查。RT-PCR和免疫组化法检测。肾组织中单核细胞化学吸引蛋白质1（MCP-1）mRNA及蛋白质的表达。结果 与糖尿病组相比，大剂量罗格列酮干预组BS、TC、TG差异无统计学意义；而。肾组织MDA含量、MCP-1mRNA及蛋白质表达、NF-κB活性差异有统计学意义（P〈0．05），SOD、GSH-Px、T-AOC活性显著上升；肾小球面积及体积减小。结论大剂量的罗格列酮可显著改善糖尿病大鼠的。肾脏损害，其机制可能与其抗氧化，抑制NF-κB活性，降低MCP-1的含量有关。     

巴柳氮对葡聚糖硫酸钠结肠炎小鼠肠黏膜通透性的影响

目的探讨巴柳氮对结肠炎小鼠肠黏膜通透性的影响及其作用机制。方法将45只C57BL／6J小鼠分成正常对照组、葡聚糖硫酸钠（DSS）模型组、巴柳氮不同剂量组（42、141、423mg／kg）,正常对照组自由饮水,其余各组自由饮用5％DSS溶液,巴柳氮灌胃给药。每日予以疾病活动指数（DAI）评分,实验结束后取结肠组织进行苏木精-伊红染色评分,匀浆检测髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、还原型谷胱甘肽活性（GSH-Px）、丙二醛（MDA）含量。小肠黏膜透射电镜检查。Evans蓝检测小肠黏膜通透性。结果与正常对照组小鼠相比,DSS组小鼠均出现明显的体质量减轻、便血和腹泻,DAI评分和病理（HI）评分增高（P〈0．01）。同时,结肠黏膜MPO活性和MDA含量明显增高（P〈0．05）,SOD和GSH-Px活性明显降低。透射电镜检查小鼠回肠黏膜绒毛变短、萎缩、稀疏和排列极不规则,细胞间连接复合体缩短、变宽及细胞间隙扩大;小肠组织中Evans蓝含量增高。巴柳氮给药组小鼠一般情况明显改善,DAI评分与HI评分降低（P〈0．05）,MPO活性减低,MDA含量降低,SOD和GSH-Px活性增高。随着巴柳氮剂量的增高,回肠黏膜微绒毛形态接近正常,小肠组织中Evans蓝含量明显减少。结论DSS模型小鼠肠黏膜通透陛增高,巴柳氮可改善结肠炎模型小鼠肠黏膜通透性。     

绞股蓝皂苷在小鼠皮肤衰老中的抗氧化损伤作用研究

目的:探讨绞股蓝皂苷对衰老小鼠皮肤的抗氧化保护作用。方法:60只小鼠随机分成3组,青年组(A)、老年对照组(B)和老年给药组(C);A、B组灌胃生理盐水20ml/kg,C组灌胃绞股蓝皂苷提取液8g/kg,每天1次。30天后取皮肤组织,检测丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果:与A组比较,B组、C组皮肤组织中MDA含量增加,SOD、CAT、GSH-Px活性降低(P<0.05);与B组比较,C组皮肤组织中MDA含量减少,SOD、CAT、GSH-Px活性增加(P<0.05)。结论:灌胃绞股蓝皂苷可减轻衰老小鼠皮肤的氧化损伤,具有延缓小鼠皮肤衰老的作用。     

Effects of melatonin on lipid peroxidation and antioxidant enzymes in streptozotocin-induced diabetic rat testis

Aim:To examine the effects of melatonin treatment on lipid peroxidation(LPO)and the activities of antioxidantenzymes in the testicular tissue of streptozotocin(STZ)-induced diabetic rats.Methods:Twenty-six male rats wererandomly divided into three groups as follows:group Ⅰ,control,non-diabetic rats(n=9);group Ⅱ,STZ-induced,untreated diabetic rats(n = 8);group Ⅲ,STZ-induced,melatonin-treated(dose of 10 mg/kg-day)diabetic rats(n=9).Following 8-week melatonin treatment,all rats were anaesthetized and then were killed to remove testes from thescrotum.Results:As compared to group Ⅰ,in rat testicular tissues of group Ⅱ,increased levels of malondialdehyde(MDA)(P<0.01)and superoxide dismutase(SOD)(P<0.01)as well as decreased levels of catalase(CAT)(P<0.01)and glutathione peroxidase(GSH-Px)(P>0.05)were found.In centrast,as compared to group Ⅱ,in rat testiculartissues of group Ⅲ,levels of MDA decreased(but this decrease was not significant,P>0.05)and SOD(P<0.01)aswell as CAT(P<0.05)increased.GSH-Px was not influenced by any of the treatment.Melatonin did not signifi-cantly affect the elevated glucose concentration of diabetic group.At the end of the study,there was no significantdifference between the melatonin-treated group and the untreated group by means of body and testicular weight.Conclusion:Diabetes mellitus increases oxidative stress and melatonin inhibits lipid peroxidation and might regulatethe activities of antioxidant enzymes of diabetic rat testes.(Asian J Androl 2006 Sep;8:595-600)     

Impact of polychlorinated biphenyl （Aroclor 1254） and vitamin C on antioxidant system of rat ventral prostate

Aim: To evaluate the effect of polychlorinated biphenyls (PCB) and vitamin C on ventral prostatic antioxidant system in adult male rats. Methods: A group of 20 adult male rats were administered ip Aroclor 1254 in corn oil at a dose of 2 mg·kg-1·day-1 for 30 days. Ten control rats were administered only the vehicle. After 30 days the treated rats were divided at random into 2 sub-groups of 10 animals each. One sub-group received vitamin C at a dose of 500 mg·kg-1·day-1 for 10 days. The other group was maintained as Aroclor 1254 control. Twenty-four hours after the last treatment the rats were killed by decapitation. Ventral prostatic homogenate was prepared and used for the estimation of enzymatic antioxidants, including superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) and hydrogen peroxide (H2O2), lipid peroxidation (LPO) and prostatic acid phosphatase. The serum levels of total T3, total T4, TSH, testosterone and estradiol were also assayed. Results: The body weight and     

Effects of endurance training on antioxidant defense mechanisms and lipid peroxidation in testis of rats

Male rats were equally divided into trained rest (TR), trained exhaustive exercise (TE), untrained rest (UR), and untrained exhaustive exercise (UE). Endurance training consisted of treadmill running for 1.5 h/d, 5 days a week for 8 weeks reaching the speed of 2.1 km/h at the fortieth week. For acute exhaustive exercise, graded treadmill running was conducted reaching the speed of 2.1 km/h at 95th min, 10% uphill, continued until exhaustion. Testicular tissue malondialdehyde (MDA), antioxidant potential (AOP) levels, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), glutathione reductase (GR) and catalase (CAT) activities were determined. There was a slight decrease, but not significant, in the SOD activity in UE group compared to TE and TR groups. Activity of GSH-Px decreased in the UE group compared to UR, TR and TE groups. Acute exhaustive exercise did not affect testicular tissue GSH-Px activity in trained rats. Testicular tissue GST activity of the UE group was similar to TE group, but lower than UR and TR groups. In UE group, testicular tissue AOP values were lower than UR, TR and TE groups. The oxidative effects of acute exhaustive exercise on the rat testis decreased with endurance training. Endurance training prevents oxidative injuries by eliminating oxygen radicals and inhibiting lipid peroxidation via preventing decreases in antioxidant enzyme activities.     

Protective effects of thymoquinone against apoptosis and oxidative stress by arsenic in rat kidney

We aimed to investigate the protective role of thymoquinone (TQ) by targeting its antiapoptotic and antioxidant properties against kidney damage induced by arsenic in rats. We have used the 24 male Sprague-Dawley rats. Rats were divided into three groups. Physiological serum in 10?mL/kg dose as intragastric was given to the control group. Sodium arsenite (10?mg/kg, intragastric by gavage for fifteen days) was given to the arsenic group. Sodium arsenite (10?mg/kg, intragastric by gavage for fifteen days) and TQ (10?mg/kg, intragastric by gavage for 15 days) was given to the arsenic?+?TQ group. After 15 days, the animals’ kidneys were taken theirs, then we have performed histological and apoptotic assessment. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzyme activities and malondialdehyde (MDA) levels have examined as the oxidative stress parameters. We have determined the levels of arsenic. Increased renal injury and apoptotic cells have been detected in the arsenic group. Degenerative changes in the arsenic?+?TQ group were diminished. Although the MDA levels were augmented in the arsenic group, SOD, CAT and GSH-Px enzyme activities were lessened than the other groups. Our findings suggest that TQ may impede the oxidative stress, the cells have been damaged and also the generation of apoptotic cells arisen from arsenic. TQ plays a protective role against arsenic-induced toxicity in kidney and may potentially be used as a remedial agent.     

Beneficial effects of chrysin on the reproductive system of adult male rats

In this study, the beneficial effect of chrysin, a natural flavonoid currently under investigation due to its important biological activities, on reproductive system of rats was investigated. Rats (n = 16) were divided randomly into two equal groups. Rats in control group were given corn oil as carrier. Chrysin was orally administered at the dose of 50 mg kg(-1) per day by gavages, and it was dissolved in corn oil for 60 days. Tissue thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) levels, antioxidant enzyme activity (CAT, SOD and GSH-Px), sperm parameters (motility, concentration and abnormal sperm rate), reproductive organ weight (testes, epididymis, vesicula seminalis, prostate) and serum testosterone levels were determined in the rats. Our results indicated that chrysin significantly increased GSH, CAT, GSH-Px and CuZn-SOD levels, but did not change the formation of TBARS significantly. In addition, sperm motility, sperm concentration and serum testosterone levels significantly increased, whereas abnormal sperm rate significantly decreased with chrysin treatment. In conclusion, it is suggested that treatment with chrysin can positively affect the reproductive system in rats, and it can be used for the treatment of male infertility.     

Erdosteine improves oxidative damage in a rat model of renal ischemia-reperfusion injury

The aim of the present study was to determine the effects of erdosteine, a new antioxidant and anti-inflammatory agent, on lipid peroxidation, neutrophil infiltration, and antioxidant enzyme activities in a rat model of renal ischemia-reperfusion (I/R) injury. Twenty-eight rats were divided into three groups: sham operation, I/R, and I/R plus erdosteine groups. After the experimental procedure, rats were sacrificed and kidneys were removed and prepared for malondialdehyde (MDA) levels, myeloperoxidase (MPO), xanthine oxidase (XO), catalase (CAT) and superoxide dismutase (SOD) activities. MDA level, MPO and XO activities were significantly increased in the I/R group. On the other hand, SOD and CAT activities were found to be decreased in the I/R group compared to the sham group. Pretreatment with erdosteine significantly diminished tissue MDA level, MPO and XO activities. Our data support a role for erdosteine in attenuation in renal damage after I/R injury of the kidney, in part at least by inhibition of neutrophil sequestration and XO activity.     

Renal antioxidant status in rats with hypertension induced by N sup omega nitro-L-arginine methyl ester

Nitric oxide (NO) has a role in the etiopathogenesis of hypertension. Relaxation of vascular smooth muscles is failed when NO production is reduced leading to increased vascular peripheral resistance. N sup omega nitro-L-arginine methyl ester (L-NAME) is one of the inhibitors of NO production. The aim of this study was to investigate oxidant-antioxidant systems of renal tissue in rats with hypertension induced by L-NAME. Rats were divided into three groups: control group and study groups treated with 100 or 500 mg/l L-NAME in drinking water for 15 days. The activities of catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), and the levels of malondialdehyde (MDA) and NO were studied in the renal tissue after hypertension induction. Arterial blood pressure was increased in both L- NAME groups. CAT activity of 500-mg L-NAME group was higher than control. GSH-Px activity of 500-mg L-NAME group was decreased compared with 100-mg ones. NO level was lower in 500-mg L-NAME group than control. MDA levels in both L-NAME groups were decreased compared with control. In conclusion, hypertension was induced with oral L-NAME treatment. Increased CAT activity was compensated with decreased GSH-Px activity in 500-mg L-NAME group. Both study groups were protected from lipid peroxidation with NO inhibition.     

The effects of cyclosporine on antioxidant enzyme activities and malondialdehyde levels in rabbit hepatic tissues

Possible molecular mechanisms leading to cyclosporine-induced hepatotoxicity has not been cleared yet. Therefore, investigation of antioxidant status of hepatic tissues exposed to cyclosporine A (CsA) and of free radical involvement in the CsA-induced hepatotoxicity seems of importance. For this aim, 20 rabbits were used in the study. In each group (control, CsA, CsA plus vitamin and, vitamin only) there were 5 animals. CsA was given orally (25 mg/kg/day) for 10 days. Vitamins E (100 mg/kg/ day) and C (200 mg/kg/day) combination was injected intramuscularly. After 10th day, animals were killed, and livers were prepared for the enzymatic assays. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) and, malondialdehyde (MDA) levels were determined in the supernatant fractions. Lowered SOD, unchanged GSH-Px and, increased CAT activities and MDA levels were detected in hepatic tissues of rabbits treated with CsA as compared with controls. In the CsA plus vitamin group, SOD activity was found to be higher, GSH-Px and CAT activities unchanged and MDA levels lower than the CsA group. In the vitamin-treated group, all of the enzyme activities were higher than the controls but MDA levels were unchanged. Correlation analysis revealed some significant differences between the groups. Results suggest that cyclosporine impairs the antioxidant defense system and thus, leads to oxidant stress and peroxidation in rabbit hepatic tissues. It has been established that this process can be prevented by antioxidant vitamin supplementation.     

罗格列酮改善老年大鼠肾脏抗氧化能力

目的 探讨罗格列酮对衰老大鼠肾脏抗氧化能力的影响。 方法 24月龄SD雄性大鼠30只,按数字随机法分成3组,每组10只：对照组（CON组）为自由摄食;罗格列酮组（RGTZ组）为自由摄食,并以罗格列酮4 mg&#8226;kg－1&#8226;d－1灌胃;限制饮食组（CR组）能量摄入为CON组的60%。干预12周后断头处死所有动物,对比各组大鼠干预前后的体质量变化;观察干预后各组大鼠的肾质量及心质量与体质量之比;Western印迹分析肾组织内过氧化物酶体增殖物激活受体γ（PPARγ）蛋白表达;检测肾组织匀浆超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活力及丙二醛（MDA）的含量;肾组织冰冻切片行β半乳糖苷酶（β-Gal）染色。 结果 干预后CR组大鼠体质量明显下降（P ＜ 0.05）,而CON组及RGTZ组无显著变化。各组大鼠干预前后肾质量与体质量比、心质量与体质量比均无显著变化。PPARγ蛋白表达在RGTZ组与CR组显著上调（P ＜ 0.01,P ＜ 0.05）。CR组及RGTZ组肾组织SOD、GSH-PX酶活力均显著高于CONT组（P ＜ 0.01）,MDA的含量均显著低于CON组（P ＜ 0.01,P ＜ 0.05）。RGTZ组、CR组β-Gal染色阳性面积明显低于CON组（P ＜ 0.05,P ＜ 0.01）。 结论 罗格列酮能够减轻衰老大鼠肾组织的氧化损伤、增强其抗氧化能力,从而起到延缓大鼠肾脏衰老的作用。