nm23—H1基因蛋白在大肠癌中表达与预后的关系

用ＬＳＡＢ免疫组化法测定２００例大肠癌组织中ｎｍ２３－Ｈ１基因蛋白，结果发现：（１）ｎｍ２３－Ｈ１在２００例大肠癌中阳性表达率２８．５％，其中在乳头状腺癌，高分化腺癌，粘膜内癌及无淋巴结转移者阳性率显著高于其它各型（Ｐ〈０．０１）。（２）ｎｍ２３－Ｈ１阳性病例比阴性病例有较高的生存率和较低的复发率（Ｐ〈０．０１）。结果表明ｎｍ２３－Ｈ１在大肠癌中的阳性表达在抑制肿瘤复发及转移中起了重要作用，可作为     

nm23—H1蛋白在乳腺浸润性癌中的表达与转移及预后的关系

应用ＬＳＡＢ免疫组织化学法，研究转移抑制基因ｎｍ２３－Ｈ１蛋白在乳腺浸润性癌的表达与转移及预后的关系。结果：１．ｎｍ２３－Ｈ１蛋白在ＩＢＣ中阳性率为６９．７６％（６０／８６），２．发生骨，肺，肝，脑等远处转移者原发灶ｎｍ２３－Ｈ１蛋白表达率５５．８８％（１９／３４）低于随访五年以上未发现转移者的表达率８７．５％（２１／２４），两者差异有显著意义（Ｐ〈０．０１）。３．ｎｍ２３－Ｈ１蛋白表达与临床分期     

原发性肝癌（PHC）中nm23—H1蛋白的表达及与预后的关系

肿瘤的转移往往是肿瘤病人死亡的直接原因，转移受其自身的转移基因及转移抑制基因的调控，ｎｍ２３作为转移抑制基因定位于人体１７号染色体长臂（１７ｑ２２），编码产物为二磷酸核苷激酶（ＮＤＰＫ），具有肿瘤转移抑制潜能。ｎｍ２３－Ｈ１蛋白与乳腺癌等肿瘤转移的关...     

宫颈癌中nm23—H1基因的不同表达与淋巴结转移及预后关系研究

目的 探讨ｎｍ２３－Ｈ１基因在宫颈癌组织中表达及其意义。方法 采用免疫组化法检测８８例宫颈癌组织中ｎｍ２３－Ｈ１基因表达;对ｎｍ２３－Ｈ１表达与临床因素及预后关系进行分析。结果 宫颈鳞癌与腺癌的表达阳性率分别为４６．４％（２６／５６）和５６．２％（１８／３２）;在与临床病理指标的相关性分析中发现：宫颈腺癌中,无淋巴结转移者ｎｍ２３－Ｈ１阳性率明显高于有淋巴结转称者（Ｐ〈０．０１）;而鳞癌中ｎｍ２３     

nm23—H1基因与乳腺癌转移和预后的关系及其突变的研究

采用免疫组化染色分析100例乳腺癌组织的nm23－H1基因表达与腋窝淋巴结转移和预后的关系，发现阳性表达者的5年无瘤生存率为84．8％（39／46），明显高于阴性表达者的52．9％（18／34），P＜0．01。进一步对20例采用RNA提取、PT－PCR、PCR－SSCP等方法检测乳腺癌组织中nm23－H1基因mRNA表达和突变情况，发现有突变的乳腺癌其基因表达均为阴性，胶窝淋巴结转移均为阳性（P＝0．036）。研究结果提示：nm23－H1基因突变可能易发生于晚期乳腺癌，同时检测nm23－H1基因mRNA表达水平和突变情况可作为判断乳腺癌（尤其是无淋巴结转移乳腺癌）的一项指标。     

nm23－H_1基因蛋白在大肠癌中表达与预后的关系

用ＬＳＡＢ免疫组化法测定２００例大肠癌组织中ｎｍ２３－Ｈ１基因蛋白，结果发现：（１）ｎｍ２３－Ｈ１在２００例大肠癌中阳性表达率２８．５％，其中在乳头状腺癌，高分化腺癌，粘膜内癌及无淋巴结转移者阳性率显著高于其它各型（Ｐ＜０．０１）。（２）ｎｍ２３－Ｈ１阳性病例比阴性病例有较高的生存率和较低的复发率（Ｐ＜０．０１）。结果表明ｎｍ２３－Ｈ１在大肠癌中的阳性表达在抑制肿瘤复发及转移中起了重要作用，可作为大肠癌患者预测转移和预后的有用指标     

nm23—H1蛋白在鼻咽癌的表达及临床意义

ｎｍ２３－Ｈ１蛋白在鼻咽癌的表达及临床意义王力红梁传余张尚福徐刚华西医科大学附属第一医院耳鼻咽喉科（成都市６１００４１）肿瘤转移是肿瘤的恶性标志和特征，对于肿瘤的预后有极重要的影响，也是肿瘤病人治疗失败和死亡的主要原因。近年发现了一类与肿瘤转移有关的...     

nm23—H1蛋白在食管鳞癌中的表达及意义

1988年，Steeg等首先分离并鉴定了一个能抑制小鼠黑色素瘤细胞转移的基因，称之为肿瘤转移抑制基因-nm23。此基因有两个亚型，nm23－H1和nm23－H2研究表明，nm23－H1在人体肝癌、胃癌及结肠癌中起更重要的作用，但在食管癌中的作用尚不清楚，本文采用免疫组织化学方法，研究nm23－H1蛋白在食管鳞癌中的表达及其临床意义。1材料与方法1．1材料取自我院1991～1995年间原发性食管鳞癌手术切除标本40例，经福尔马林固定后，石蜡包埋，常规制备印m切片。按国际卜TNM分期，I期1例．I期11例，皿期22例，IV期6例。肿瘤分化程度分级：I级（高分…     

肾癌nm23—H1nRNA表达与浸润转移的关系

为探讨nm23-H1在肾癌组织中的表达状况,我们采用RT-PCR方法检测25例肾癌及癌周组织中nm23-H1mRNA的表达,并探讨nm23-H1mRNA的表达与肾癌浸润转移的关系.结果报告如下:     

肿瘤转移抑制基因nm23—H1在肾癌中的表达

我们应用免疫组织化学（ＬＳＡＢ）方法检测ｎｍ２３Ｈ１基因在肾癌中的表达情况,以探讨其在肾癌发生发展中的作用。１　材料和方法收集１９８０年至１９９１年肾癌存档标本５６例,男性３６例,女性２０例,平均年龄５７．２岁。标本经１０％福尔马林固定,石蜡包埋。按ＴＮＭ分期：Ⅰ期３例,Ⅱ期１９例,Ⅲ１４例,Ⅳ期２０例。Ｆｕｈｒｍａｎ核分级：Ｇ１级２３例,Ｇ２级２４例,Ｇ３级９例。另选１０例癌旁组织作为对照。石蜡切片脱蜡、水化,０．３％Ｈ２Ｏ２室温１０ｍｉｎ阻断内源性过氧化物酶,１０％硫酸铝钾液微波内辐射２ｍ…     

nm23-H1与大肠癌微转移关系的研究

目的探讨nm23-H1基因表达与大肠癌浸润、微转移和预后的关系。方法经病理确诊的120例大肠癌组织标本,采用SP法检测nm23-H1基因蛋白表达、巢式RT-PCR方法检测进入外周静脉血的大肠癌微转移灶(CK20mRNA),并与同一患者大肠癌组织标本中nm23-H1基因表达情况进行综合分析。结果nm23-H1在大肠癌中的阳性表达率为61.7%(74/120),低分化腺癌中nm23-H1阳性表达率(41.0%)显著低于高中分化腺癌中的表达率(70.3%)(P<0.05);有淋巴结转移者nm23-H1阳性表达率为53.7%,无淋巴结转移者为71.7%,两组比较有显著性差异(P<0.05);120例大肠癌患者术后3年随访中,CK20mRNA阳性检出率为41.5%,nm23-H1阳性表达率与CK2OmRNA阳性率呈显著负相关(P<0.001);nm23-H1蛋白阳性表达组术后3年生存率为60.8%(45/74),阴性表达组术后3年生存率为21.7%(10/46),二组比较有显著性差异(P<0.001)。结论nm23-H1与大肠癌细胞分化、淋巴结转移及外周静脉血中微转移密切相关,是影响大肠癌预后的重要指标之一。     

Expression of nm23-H1 and nm23-H2 protein in endometrial carcinoma.

nm23 gene expression has been shown to be inversely correlated with tumour metastatic potential in some cancers but not in others. Examination was made of the expression of nm23-H1 and nm23-H2 gene products by immunohistochemistry and immunoblotting in 28 endometrial carcinomas. Immunohistochemistry indicated the cytoplasm of cancer cells to be positive, and myometrium and endometrial stromal cells negative, for nm23-H1 and -H2 protein. The staining intensity for these proteins was significantly stronger in well-differentiated adenocarcinomas (G1) than in those moderately differentiated (G2) (P < 0.05). nm23-H1 and -H2 proteins were shown by immunoblotting to be present at significantly higher levels in G1 than in G2 tumours (P < 0.05). Two of eight cases expressed high nm23-H1 and -H2 protein in poorly differentiated adenocarcinomas (G3). In G3 tumours, nm23 expression may be diverse. In this study, the expression of nm23-H1 and -H2 was not correlated with stage, metastasis, tumour size, myometrial invasion, oestrogen receptor, progesterone receptor or menopause. It follows from the findings presented above that the high expression of nm23-H1 and -H2 is positively correlated with histological differentiation.     

BRCA1和nm23-H1与乳腺癌预后的关系

目的 :探讨BRCA1、nm2 3 H1在乳腺癌预后中的作用及相互关系。方法 :对 10 6例乳腺癌患者的肿瘤组织石蜡切片采用免疫组织化学染色 (S P法 )技术标记BRCA1、nm2 3 H1。结果 :乳腺癌组织中BRCA1、nm2 3 H1蛋白表达明显降低 ,与生存期呈正相关 ,与复发呈负相关 (P <0 0 5 )。BRCA1与nm2 3 H1蛋白表达水平呈正相关 (P <0 0 5 )。结论 :BRCA1、nm2 3 H1在乳腺癌组织内共同参与癌组织的浸润和转移 ,可作为预后的判断指标     

Expression of nm23-H1 gene product in nasopharyngeal carcinoma and its clinical significance

Objective: The nm23 gene is one of the tumor metastatic suppressor genes. The expression of nm23-H1 has been reported to be inversely associated with metastatic potentiality in a number of human carcinomas, including breast, colorectal, gastric, hepatocellular and gallbladder carcinomas. In this study, the immunohisto-chemical staining of nm23-H1 protein in human naso-pharyngeal carcinoma (NPC) was examined, and the relationship between nm23-H1 and both metastasis and prognosis of patients with NPC was also investigated. Methods: Routine LSAB immunohistochemistry with the nm23-H1 monoclonal murine antibody was employed to study the expression of nm23-H1 protein in 95 paraffin-embedded specimens of NPC treated at our hospital. The clinical pathologic data and results of follow-up were also retrieved. Comparisons between patients with and without expression of nm23-H1 protein with respect to metastasis, loco-regional recurrence and survival were performed using Log rank test. Multivariate prognostic analyses were performed by using Cox’s regression model. Results: Nm23-H1 negative expressive tumors were associated with a higher incidence of lymph-node metastasis (86.7%) than those of nm23-H1 positive (48.6%,P<0.01). Nm23-Hl negative expressive tumors were associated with a high incidence of recurrence and distant metastasis after radiotherapy (P<0.05). A significant association was found between expression of nm23-H1 and prognosis (P<0.01). The expression of nm23-H1 indicated favorable prognosis. Conclusion: It was suggested that nm23-H1 negative expression was significantly associated with lymph-node metastasis, recurrence and distant metastasis. Nm23-H1 may have value for predicting the prognosis of NPC.     

Inverse association of nm23-H1 expression by colorectal cancer with liver metastasis.

The expression of nm23-H1 mRNA and protein was studied in colorectal cancers by Northern blotting and immunohistochemistry. All 21 colorectal cancers studied by Northern blotting had increased levels of nm23-H1 mRNA relative to the adjacent normal colonic mucosa. Increased nm23-H1 protein expression was also observed in all 36 colorectal cancer cases including those studied by Northern blotting. There was no significant correlation between nm23-H1 expression and tumour histology, serosal invasion, lymphatic invasion, venous invasion, or lymph node metastasis. However, the expression of both mRNA and protein was significantly lower in tumours associated with liver metastasis than in those without such metastasis. These observations indicate that the nm23 gene may play a role in the suppression of liver metastasis of colorectal cancer.     

肿瘤血管生成与nm23基因表达及胃癌淋巴结转移的关系

目的 研究肿瘤血管生成与转移抑制基因ｎｍ２３－Ｈ１的表达及胃癌淋巴结转移的关系。方法 对９３例胃癌手术切除标本,检测ｎｍ２３Ｈ１基因蛋白的表达,分析微血管密度和血管上皮生长因子的表达情况。结果 伴有淋巴结转移组ＭＶＤ、ＶＥＧＦ指数明显高于不伴有淋巴结转移组,而ｎｍ２３－Ｈ１的表达则相反同时ｎｍ２３低表达组的ＭＶＤ显著高于ｎｍ２３高表达组,Ｐ〈０．０５。结论ＭＶＤ、ＶＥＧＦ和ｎｍ２３在胃癌淋巴细胞     

Expression of nm23-H1 in uveal melanoma

Uveal melanoma (UM) is the most common malignant intraocular tumor in adults. Despite the high accuracy of clinical diagnosis and advances in local treatment, more than 50% of UM patients develop metastasis within 10 years of initial diagnosis. NM23 is one of the human metastasis suppressor genes. Reduced nm23-H1 expression is correlated with high metastatic potential in many different cancers. The purpose of this study is to investigate the expression of nm23-H1 in UM and its potential value as a prognostic marker. Immunostaining of nm23-H1 was verified in five human UM cell lines with different metastatic potentials. The expression level of nm23-H1 mRNA was evaluated with one-step quantitative real-time PCR. The invasion ability of the cell lines was assessed before and after silencing nm23-H1 with small interference RNA. Thirty-two cases of paraffin-embedded specimens of human UM were immunostained with nm23-H1 monoclonal antibody. The immunostaining was evaluated in a semiquantitative fashion based on extent and intensity. The real-time PCR results of five human UM cell lines showed that expression of nm23-H1 was higher in cell lines with low metastatic potential compared with those with high metastatic potential (P<0.05). The invasive ability of the UM cell lines increased after silencing nm23-H1 expression with small interference RNA (P<0.05). The immunostaining of nm23-H1 was cytoplasmic in all cell lines and UM patients samples. The increased immunostaining intensity of nm23-H1 in patients' samples was associated with better survival rate (Kaplan-Meier test P=0.0097). The expression of nm23-H1 was not correlated with other prognostic factors. It can be concluded that nm23-H1 may be a prognostic marker to predict the survival rate of UM patients and it has the potential to identify high-risk patients.     

核因子-κB和nm23-H1在肝细胞肝癌中的表达及其意义

目的探讨核因子NF-κB和转移抑制基因nm23-H1在肝细胞癌（HCC）组织的表达情况及与临床病理特征的关系。方法采用免疫组织化学方法检测41例HCC中NF-κB和nm23-H1的表达情况，以相应癌旁肝组织、肝硬化组织（10例）、肝血管瘤旁正常肝组织（11例）作对照，并分析其与病理特征的关系。结果NF-κB蛋白在41例HCC组织中阳性28例，表达率为68．3％；41例相应癌旁肝组织中有5例表达，阳性率12．2％，10例肝硬变组织中1例表达，11例正常肝组织未见表达，HCC与非癌组织中NF-κB蛋白的表达差异有统计学意义（χ^2=41．1，P〈0．01）；NF-κB的表达与HCC的临床病理特点无显著相关性。nm23-H1蛋白在41例HCC组织中14例为阳性染色，27例为阴性染色。nm23-H1阴性表达与肝被膜浸润和门静脉浸润显著相关（χ^2=4．16，χ^2=4．19，P均〈0．05）。结论NF-κB可能参与了HCC的发生、发展，可望作为基因治疗的靶点。nm23-H1在HCC中的阴性表达与HCC的恶性生物学特征相关，可能表明肿瘤的预后不良。     

p53和nm23-H1蛋白表达与食管癌浸润转移的关系

Objective To study the relationship between expression of p53 and nm23-H1 and differentiation, invasiveness and metastasis in human esophageal carcinoma, and the correlation between expression of p53 and nm23-H1. Methods Expression of p53 and nm23-H1 in 50 patients with squamous cell carcinoma of esophagus was detected by using immuno-histochemical S-P methods. Results 35 cases (70%) and 32 cases (64%) of esophageal squamous cell carcinoma were positive for nm23-H1 protein and p53 protein, respectively. The expression of nm23-H1 was related to lymphatic metastasis (P<0.025), but not related to tumor differentiation, invasiveness, tumor location, tumor length, patient's gender and age (P>0.05). The lymphatic metastasis location positive group had a very lower expression of nm23-H1 and the negative rate was 70.8%, but the negative group had a higher expression and the positive rate was 65.4%. The expression of p53 was related to tumor differentiation and invasiveness (P<0.05), but not related to lymphatic metastasis, tumor location, tumor length, patient's gender and age(P>0.05). Among the three groups, the high differentiation group had the lowest expression of p53 and the positive rate was 29.2%, but the low differentiation group had the highest positive rate (71.4%). As for tumor invasiveness, the group of outer membrane of esophagus infiltrated had the highest p53 protein positive rate (56%), but in the group, of mucous or submucous layer infiltrated p53 protien was not detectable. The low expression of nm23-H1 and the high expression of p53 were also correlated. The expression of nm23-H1 and p53 were both correlated with TNM stage of esophageal carcinoma (P<0.05). The better esophageal carcinomas differentiated, the lower nm23-H1 expressed and higher p53 expressed. Conclusion Low expression of nm23-H1 and high expression of p53 play an important role in the progression of squamous cell carcinoma of esophagus. Nm23-H1 might beta gene markef in the prophecy of patients' prognosis and benefit tumor treatment clinically.