食管鳞癌淋巴管生成因子表达与淋巴结转移的关系

目的:探讨淋巴管生成因子(VEGF-C)的表达与食管鳞癌淋巴结转移的关系.方法:采用免疫组化法检测49例食管鳞癌组织及其周围正常食管组织中VEGF-C的表达,并结合食管鳞癌的淋巴结转移特征进行分析.结果: VEGF-C在食管鳞癌组织和对应正常组织中阳性表达率分别为71.4%和12.2%.有淋巴结转移组(N1)VEGF-C蛋白阳性表达率73.3%,明显高于无淋巴结转移组(N0)32.6% (P<0.05).结论: 食管鳞癌组织中存在VEGF-C的高表达,可能与食管癌的淋巴结转移有关.VEGF-C蛋白表达可能作为推断食管鳞癌淋巴结转移的参考指标.     

VEGF-C和VEGF-D在胃癌及相关淋巴结组织中表达和临床意义的研究

目的：比较血管内皮生长因子C（vascular endothelial growth factor C,VEGF-C）和血管内皮生长因子D（vascular endothelial growth factor D,VEGF-D）在胃癌和相关淋巴结的表达,并评价其预后意义.方法：采用原位杂交技术检测45例胃癌患者原发病灶和转移淋巴结中VEGF-C和VEGF-D mRNA的表达水平;并检测转移及非转移淋巴结中VEGF-C和VEGF-D mRNA表达差异.结果：71%和62.2%的胃癌原发病灶可见VEGF-C和VEGF-D mRNA表达,原发病灶阳性者,相应的淋巴结转移病灶都可见VEGF-C和VEGF-D mRNA表达,55.6%和51.1%的胃癌患者淋巴结转移病灶VEGF-C和VEGF-D mRNA表达水平高于原发病灶,原发病灶和转移病灶淋巴管生成表达水平的差异与胃癌的预后密切相关（P＜0.05）.结论：胃癌癌细胞转移至淋巴结后淋巴管生成因子表达可能进一步提高,以进一步增强转移能力.     

食管鳞癌组织VEGF-C和VEGF-D表达及其与淋巴管生成和转移关系的探讨

目的:探讨食管鳞癌组织中血管内皮生长因子C(vascular endothelial growth factor-C,VEGF-C)、血管内皮生长因子D(vascular endothelial growth factor D,VEGF-D)的表达及与淋巴管生成和淋巴结转移的关系.方法:采用免疫组化法检测64例食管癌和14例癌旁正常食管黏膜中VEGF-C、VEGF-D和D2-40的表达,并分析其与临床病理诸因素的关系.结果:VEGF-C在淋巴结转移组和无淋巴结转移组的阳性率分别为73.08%(19/26)和34.21%(13/38),VEGF-D的阳性率分别为53.85%(14/26)和28.95%(11/38),差异有统计学意义,x2值分别为9.328和4.021,P值分别为0.002和0.045.VEGF-C和VEGF-D之间的表达差异无统计学意义,x2=1.641,P=0.20.癌组织和癌旁正常食管黏膜中D2-40阳性表达的淋巴管密度(lymphatic-vessel density, LVD)差异有统计学意义,P=0.000;VEGF-C阳性组与阴性组中D2-40阳性表达的LVD差异有统计学意义,P=0.010,而VEGF-D阳性组与阴性组中差异无统计学意义.P=0.543.结论:在食管癌中存在VEGF-C、VEGF-D的表达,VEGF-C通过增加癌周LVD促进肿瘤细胞的淋巴结转移,与VEGF-D各自参与淋巴结转移的作用.两者的表达预示淋巴结转移的增加,对食管癌预后估计有指导意义.     

食管鳞状细胞癌组织中COX-2、VEGF-C的表达及其与淋巴管生成的关系

摘 要　目的：探讨人食管鳞状细胞癌组织中环氧合酶2（cyclooxygenase-2,COX-2）和血管内皮生长因子C（vascular endothelial growth factor-C,VEGF-C）的表达及其与肿瘤淋巴管生成的关系。方法：取2002年1月至2007年1月间辽宁医学院附属第一医院病理科66例食管鳞状细胞癌组织标本,另取10例癌旁组织标本（食管癌病灶旁2 cm外黏膜）作为对照。采用免疫组化SP法检测食管鳞状细胞癌组织中COX-2、VEGF-C的表达情况;采用血管内皮生长因子受体3（VEGFR-3）和基底膜标记物Ⅳ型胶原免疫组化染色观察肿瘤组织淋巴管生成情况,测量淋巴管密度（lymphatic vessel density,LVD）,并分析其与淋巴结转移的关系。结果：66例食管鳞癌组织中COX-2和VEGF-C表达阳性率分别为69.70%和56.06%,均显著高于相应的癌旁组织（P<0.05）;淋巴结转移组织中的COX-2和VEGF-C表达阳性率明显高于无淋巴结转移组织（P<0.01）;COX-2和VEGF-C表达之间存在明显相关性(r= 0.479,P<0.05)。COX-2和VEGF-C蛋白双阳性的食管鳞癌组织中LVD明显高于均呈双阴性表达者（P<0.05）。 结论：人食管鳞状细胞癌组织中存在COX-2和VEGF-C的高表达,COX-2可能通过上调VEGF-C表达促进食管鳞癌组织中淋巴管生成,进而促进淋巴结转移的发生。     

VEGF-C和VEGF-D诱导结直肠癌癌周淋巴管生成

目的:探讨结直肠癌(colorectal cancer,CRC)组织淋巴管生成情况与血管内皮生长因子-C(vascular endothelialgrowth factor-C,VEGF-C)和VEGF-D的关系。方法:应用淋巴管特异标志podoplanin检测96例结直肠癌及正常结直肠组织中淋巴管密度,以CD34标志血管作对比研究,并分别与ki-67进行双标免疫组化染色检测淋巴管和血管增殖活性;免疫组化SP法检测VEGF-C和VEGF-D的表达。结果:VEGF-C和VEGF-D表达与结直肠癌微淋巴管密度(microlymphatic den-sity,MLD)密切相关(P<0.05,P<0.01),尤以癌组织边缘区为甚(P<0.01),并且VEGF-C和VEGF-D高表达的结直肠癌组织中淋巴管内皮细胞ki-67指数也显著增加(P<0.01)。结论:结直肠癌组织存在新生淋巴管;VEGF-C和VEGF-D高表达可能诱导结直肠癌尤其癌周淋巴管生成。     

子宫内膜癌VEGF-D、MLD的表达及其与淋巴结转移的关系

目的 探讨子宫内膜癌组织中血管内皮生长因子D(VEGF-D)和微淋巴管密度(MLD)的表达与淋巴结转移的关系.方法 选取子宫内膜癌标本53例,正常子宫内膜标本20例,以淋巴管特异性标记物D2-40标记淋巴管并计数MLD,运用免疫组织化学技术检测VEGF-D和MLD在子宫内膜癌、正常内膜组织中的表达.结果 (1)VEGF-D和MLD在子宫内膜癌组织中的阳性表达率及密度值(39/53,23.54±7.84)均显著高于正常内膜组织(5/20,10.48±1.62)(P<0.05);两者在淋巴结转移组的阳性表达率及密度值(33/38,32.05±3.27)也明显高于无淋巴结转移组(6/15,20.23±4.57)(P<0.05);VEGF-D的表达在不同年龄、不同组织分化程度、不同浸润深度之间差异均无统计学意义(P>0.05),在临床分期的表达中差异有统计学意叉(P<0.05).(2)子宫内膜癌VEGF-D的表达与MLD存在相关性.相关系数γs=0.398(P<0.05),VEGF-D阳性组MLD(31.17±2.82)较VEGF-D阴性组MLD(16.75±3.63)显著增高(P<0.05).结论 VEGF-D在子宫内膜癌中呈高表达且与微淋巴管密度(MLD)呈正相关,其可能通过诱导淋巴管生成促进子宫内膜癌淋巴结转移,可作为指导子宫内膜癌诊治及判断预后的一个重要指标.     

胃癌组织中血管内皮生长因子的表达对血管和淋巴管生成的影响及其预后意义

目的 研究胃痛组织中血管内皮生成因子(VEGF)-A、VEGF-C和VEGF-D的表达对血管和淋巴管生成的影响及其预后意义.方法 采用免疫组化法检测123例原发性胃癌组织中VEGF-A、VEGF-C和VEGF-D的表达,采用D2-40和CD34免疫组化双染法分别标记淋巴管和血管,并测定淋巴管密度(LVD)和血管密度(MVD).采用单因素分析VEGF-A、VEGF-C和VEGF-D表达与胃癌临床病理特征的关系;采用Kaplan-Meier法和Log rank检验评价VEGF-A、VEGF-C和VEGF-D表达与胃癌患者预后的关系,并用Cox比例风险模型进行多因素分析.结果 123例胃癌组织中,VEGF-A、VEGF-C和VEGF-D的高表达率分别为64.2%、65.9%和41.5%.VEGF-A、VEGF-C或VEGF-D高表达及两两高表达均与LVD有关(均P＜0.05);VEGF-A和VEGF-C高表达还与浸润深度、淋巴管浸润、静脉浸润、淋巴结转移和MVD有关(均P＜0.05);VEGF-C和VEGF-D高表达均与淋巴管浸润和淋巴结转移有关(均P＜0.05).VEGF-A、VEGF-C或VEGF-D高表达者的生存时间均明显短于其低表达者(均P＜0.05),其中VEGF-A和VEGF-C均高表达者的生存时间最短(56个月).VEGF-A的表达水平、MVD、淋巴结转移及浸润深度是影响胃癌患者预后的独立因素.结论 VEGF-A和VEGFC均高表达的胃癌有更强的促进血管和淋巴管生成的能力,并可促进肿瘤转移和影响患者预后;VEGF-C和VEGF-D可共同介导淋巴管生成,促进淋巴结转移;但仅VEGF-A高表达是影响患者预后的独立危险因素.     

VEGF-C和VEGF-D在胰腺癌淋巴转移中的意义

目的 分析胰腺癌肿瘤中心和肿瘤周边组织中血管内皮生长因子(VEGF)-C、VEGF-D与微血管密度(MVD)、微淋巴管密度(MLVD)的关系,探讨VEGF-C、VEGF-D在胰腺癌淋巴结转移及发展中的意义.方法 免疫组织化学法检测30例胰腺癌组织中VEGF-C、VEGF-D、VEGF受体(VEGFR)-3、CDM蛋白的表达情况.结果 30例胰腺癌中肿瘤周边部位VEGF-C、VEGF-D蛋白阳性率分别为73.3%和56.7%,显著高于肿瘤中心部位(30.0%和16.7%,P<0.01).VEGF-C、VEGF-D高表达的肿瘤周边部位淋巴结转移、淋巴管和血管浸润显著增加(P<0.01).VEGF-C蛋白阳性组MVD高于阴性组,MLVD显著高于阴性组(P<0.01),淋巴结转移增多;VEGF-D蛋白阳性组与阴性组相比MVD无变化(P=0.07),MLVD高于阴性组(P<0.01),淋巴结转移增加.结论 胰腺癌中肿瘤周边区域中VEGF-C、VEGF-D的表达与患者淋巴结转移显著相关,并介导其淋巴管生成;而VEGF-C可能主要参与胰腺癌的血管生成和淋巴管生成的调节,VEGF-D可能仅参与其淋巴管生成的调节.     

胆囊癌组织中血管内皮生长因子C和D的表达及其与淋巴管和血管生成的关系

目的 探讨胆囊癌组织中血管内皮生长因子C(VEGF-C)和VEGF-D的表达及其与淋巴管和血管生成的关系.方法 采用免疫组化法检测50例胆囊癌组织中VEGF-C、VEGF-D、D2-40和CD31的表达,以10例癌旁正常胆囊组织和19例慢性胆囊炎作为对照,并分析其与临床病理诸因素的关系.结果 50例胆囊癌组织中,32例VEGF-C表达阳性,阳性率为64.0%,31例VEGF-D表达阳性,阳性率为62.0%,均高于癌旁正常组织(P<0.05),但与慢性胆囊炎差异无统计学意义(P>0.05).VEGF-C的表达与胆囊癌患者的年龄和淋巴结转移有关,VEGF-D的表达仅与胆囊癌患者的淋巴结转移有关.50例胆囊癌组织的微淋巴管密度(MLVD)和微血管密度(MVD)分别为6.94±3.6和36.1±12.8.VEGF-C阳性组和VEGF-D阳性组的MLVD和MVD均高于阴性组.MLVD与淋巴结转移有关,MVD与淋巴结转移、分化程度有关.VEGF-C与VEGF-D的表达呈正相关(r=0.498,P<0.01).结论 在胆囊癌中,VEGF-C和VEGF-D参与胆囊癌的淋巴生成和血管生成的调节,通过增加瘤周淋巴管的密度促进肿瘤细胞的淋巴结转移.     

食管鳞癌中VEGF—C的表达及其与淋巴血道转移和预后的关系

目的探讨血管内皮生长因子-C在食管鳞癌组织中表达及其与食管鳞癌淋巴血道转移和预后的关系.方法回顾性分析43例术前未进行化疗和放疗的食管鳞癌患者的切除标本,采用免疫组化S-P法检测食管鳞癌组织中VEGF-C的表达.结果食管鳞癌中VEGF-C的阳性表达率为67.4%(29/43),VEGF-C的阳性表达与淋巴结转移、食管癌的病理分期以及手术后血性转移显著相关(P=0.003,P＝0.012).VEGF-C阳性表达者3年生存率为23.4%,5年生存率为18.7%;阴性表达者3年生存率为60.7%,5年生存率为59.8%,两者差异有显著性(P<0.05).结论 VEGF-C与食管鳞癌的侵袭、淋巴结转移、手术后血性转移以及预后有一定关系.     

The Expression of High Mobility Group Box 1 is Associated with Lymph Node Metastasis and Poor Prognosis in Esophageal Squamous Cell Carcinoma

The objective is to explore the expression of high mobility group box 1 (HMGB1) in esophageal squamous cell carcinoma (ESCC) and its relationship with lymph node metastasis and the prognosis of patients as well as possible mechanism. The expression of HMGB1, vascular endothelial growth factor C (VEGF-C) and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1) in ESCC tissues, which were obtained from 72 patients who underwent radical esophagectomy, was detected through immunohistochemistry, firstly. The correlations between HMGB1 and VEGF-C, and micro-lymphatic vessel density (MLD), and lymph node metastasis, and the prognosis of patients, were analyzed by statistic analysis. The plasmid of small interference RNA (siRNA) targeting HMGB1, giving siHMGB1, was transfected into exponentially growing KYSE150 human esophageal squamous cancer cells and the expression of HMGB1 mRNA and protein was observed by Real-time PCR and Western Blot and the expression of VEGF-C was examined by ELISA. HMGB1 expressed highly in the nuclei and cytoplasm of carcinoma cells as well as the extracellular space in ESCC and was associated with lymph node metastasis, MLD, the expression of VEGF-C, TNM stage and the prognosis of patients (P?相似文献   

Vascular endothelial growth factor C expression correlates with lymphatic involvement and poor prognosis in patients with esophageal squamous cell carcinoma

Vascular endothelial growth factor C (VEGF-C), a member of VEGF gene family, is considered to induce both lymph node metastasis and lymphatic involvement in various cancers related to lymphangiogenesis. We examined the relationship between VEGF-C expression and clinicopathological features in 112 Japanese patients with esophageal squamous cell carcinoma (ESCC) who underwent esophagectomy and reconstruction without preoperative treatment. VEGF-C expression was examined using immunohistochemical staining and RT-PCR in surgically resected tissues. Regarding VEGF-C there were positive findings in 44 (39.3%) cases. VEGF-C expression in ESCC significantly correlated with the depth of tumor (p=0.0131), lymph node metastasis (p=0.0211), lymphatic involvement (p<0.0001) and pathological stage (p=0.0164). The prognosis for the VEGF-C positive group was poorer than that for the VEGF-C negative group (p=0.038). Multivariate analysis indicated that VEGF-C expression in ESCC is an independent factor only in cases of lymphatic involvement. VEGF-C expression in ESCC may play a great key role in lymphatic spread and this may be a consideration in terms of deciding the most appropriate treatment.     

VEGF-C and VEGF-D Expression and its Correlation with Lymph Node Metastasis in Esophageal Squamous Cell Cancer Tissue

Background: To explore vascular endothelial growth factor C (VEGF-C) and VEGF-D expression andits correlation with lymph node metastasis in esophageal squamous cell cancer (ESCC) tissue. Materials andMethods: Immunohistochemical methods were applied to detect the levels of VEGF-C and VEGF-D expressionin 64 surgicall removal ESCC tissues, tissues adjacent to cancer and normal tissues, and the relationship betweenVEGF-C and VEGF-D expression and lymph node metastasis was analyzed. Results: Both VEGF-C and VEGF-Dwere expressed by varying degrees in esophageal cancer tissue, the tissue adjacent to cancer and normal tissue,and the positive expression rate went down successively. The positive expression rates of VEGF-C (59.4%) andVEGF-D (43.8%) in esophageal cancer tissue were significantly higher than in the tissue adjacent to cancer(34.4%, 15.6%) and normal tissue (20.3%, 12.5%), respectively, in which significant differences were manifested(p<0.01). Positive expression rates of VEGF-C and VEGF-D in esophageal cancers with lymph node metastasiswere markedly higher than without such metastasis (p<0.01), while those in the tissue with TNM staging I~IIwere markedly lower than that with TNM staging III~IV (p<0.01). Conclusions: Both VEGF-C and VEGF-Dare highly expressed in ESCC tissue, which may be related to the lymph node metastasis of cancer cells. Hence,VEGF-C and VEGF-D can be clinically considered as important reference indexes of lymph node metastasis inesophageal cancer.