木果楝种子中一个新柠檬苦素类化合物

目的 研究红树林植物木果楝Xylocarpus granatum种子的化学成分。 方法 采用多种色谱方法分离纯化，依据理化性质、波谱数据分析进行结构鉴定。 结果 从木果楝种子的95%乙醇提取物中分离鉴定了一个墨西哥交酯型柠檬 苦素类化合物xylogranatin S，并利用MTT法研究了这个化合物对人子宫颈癌细胞HeLa、肝 癌细胞HLE、乳腺癌细胞MDA-MB-231和结肠癌细胞SW-620的生长抑制作用。 结论 该化合物为新的化合物，命名为木果楝亭S(xylogranatin S)，药 理活性实验结果未发现其对上述肿瘤细胞有显著的抑制作用。     

红树林植物木果楝化学成分的研究

目的 研究红树林植物木果楝Xylocarpus granatum的化学成分。方法 采用色谱法分离,用波谱方法进行结构鉴定。结果 从木果楝种子中分离鉴定了10个化合物,分别为过氧化麦角甾醇(Ⅰ)、木果楝素G(xylocarpin G,Ⅱ)、木果楝素H(xylocarpin H,又名granaxylocarpin B,Ⅲ)、xylogranatin C(Ⅳ)、3β-detigloyoxy-2′-methylbutanoyloxy xylogranatin B(Ⅴ)、木果楝亭D(xylogranatin D,Ⅵ)、硬毛鸕鸪花醇(hispidol B,Ⅶ)、东莨菪亭(Ⅷ)、β-谷甾醇(Ⅸ)和胡萝卜苷(Ⅹ)。结论 化合物Ⅰ、Ⅶ和Ⅷ为首次从木果楝属植物中分离得到。     

紫丁香籽外壳的化学成分研究

摘 要：目的 研究紫丁香Syringa oblata 籽外壳醋酸乙酯提取物的化学成分。方法 采用硅胶柱色谱和高效液相色谱进行分离纯化,通过薄层色谱及波谱数据分析进行结构鉴定。结果 分离得到了11个单体化合物,结构鉴定为丁香苦素A(1)、丁香苦素B(2)、丁香苦苷(3)、橄榄苦苷(4)、(8E)-ligustroside(5)、对羟基苯乙醇(6)、3,4-二羟基苯乙醇(7)、对羟基苯乙醇乙酸酯(8)、对羟基苯乙醇葡萄糖苷(9)、(+)-丁香树脂酚(10)、(+)-落叶松脂醇(11)。结论 化合物1为新化合物,命名为丁香苦素A(syringopicrogenin A),其余化合物均为首次从紫丁香籽外壳中分离得到。     

大叶山楝的化学成分研究

目的:对大叶山楝(Aphanamixis grandifolia)的枝叶部分进行化学成分研究。方法:对大叶山楝枝叶的95%乙醇提取物分别经石油醚和乙酸乙酯萃取。乙酸乙酯部分反复利用正相硅胶、反相RP-18、凝胶Sephadex LH-20等色谱方法进行分离纯化,并运用MS和NMR来鉴定化合物的结构。结果:从大叶山楝中分离鉴定了6个化合物,分别为:aromadendrane-4β,10β-diol(I),spathulenol(Ⅱ),eudesm-4(15)-ene-1β,6α-diol(Ⅲ),3α-cimnamoyloxykaur-16-en-19-oic acid(Ⅳ),phillygenin(V),3-methoxybenzoic-4-hydroxy acid(Ⅵ)。结论:6个化合物均为从该属植物中首次分离得到。     

常山胡柚皮的化学成分分离鉴定(英文)

目的:分析常山胡柚(Citrus changshan-huyou Y.B.Chang)皮的化学成分.方法:利用反复硅胶柱色谱分离和纯化,并通过波谱分析鉴定化合物结构.结果:分离并鉴定了10个化合物结构,分别为胡柚皮甲素(Ⅰ)、3-羟基-4-甲氧基苯甲酸(Ⅱ)、3,4-二羟基苯甲酸(Ⅲ)、脂肪酸(Ⅳ)、甘油酯(Ⅴ)、6,7-二甲氧基香豆素(Ⅵ)、6',7'-二羟基香柠檬亭(Ⅶ)、胡萝卜苷A(Ⅷ)、胡萝卜苷B(Ⅸ)和胡柚三萜(Ⅹ).结论:胡柚皮甲索为新化合物;这10个化合物均为首次从该植物中分得.     

印楝种子及叶的化学成分研究

目的:对植物印楝种子及叶的化学成分进行研究.方法:采用波谱和化学方法对印楝种子及叶的化学成分进行鉴定.结果:从印楝种子及叶中分离得到9个化合物,其结构分别确定为印楝素(1),11-表印苦楝素H(2),Salannolide(3),槲皮素-3-O-β-D-木糖苷(4),槲皮素-3-O-β-D-葡萄糖苷(5),山柰酚-3-O-(-D-葡萄糖苷(6),芦丁(7),硬脂酸 1-甘油单脂(8)和硬脂酸1,3-甘油二脂(9).结论:化合物3～化合物9为首次从该植物中分离得到.     

倒提壶化学成分研究

目的研究民族药倒提壶（Cynoglossum amabile）的化学成分。方法用硅胶色谱技术分离化学成分,经理化常数测定、波谱解析等方法进行结构鉴定。结果分离得到7个化合物,鉴定了其中5个,分别为豆甾醇（stigmasterol,1）、正二十八烷酸（octacosanoic acid,2）、胡萝卜苷（daucosterol,3）、邻羟基苯甲酸（o-hydroxybenzoic acid,4）和3,4-二羟基苯甲酸（3,4-dihydroxybenzoic acid,5）。结论化合物2、4、5为首次从该属植物中分离得到。     

过山蕨中有机酸类化学成分研究

目的研究过山蕨Camptosorussibiricus中有机酸类化学成分。方法采用反复硅胶柱色谱分离纯化,通过理化常数测定和光谱分析鉴定其化学结构。结果从过山蕨中分离得到了10个有机酸类化合物,即11,12,15-三羟基-13-烯十八碳酸(11,12,15-trihydroxy-13-en-octadecenoic acidⅠ)、咖啡酸(caffeic acid,Ⅱ)、香豆酸(courmaricacid,Ⅲ)、原儿茶酸(protocatechuic acid,Ⅳ)、对羟基苯甲酸(4-hydroxybenzoic acid,Ⅴ)、异香草酸(isovanillic acid,Ⅵ)、2,4-二羟基苯甲酸(2,4-dihydroxybenzoic acid,Ⅶ)、肉桂酸(cinnamicacid,Ⅷ)、丁二酸(succinic acid,Ⅸ)、棕榈酸(palmiticacid,Ⅹ)。结论化合物Ⅰ为新化合物,将其命名为过山蕨酸(camptosoric acid),化合物Ⅲ~Ⅹ均为首次从该属植物中分离得到。     

锦灯笼中酸浆苦素类化学成分的研究

目的 研究锦灯笼95%乙醇提取物的石油醚、氯仿、醋酸乙酯萃取部位中化学成分。方法 利用硅胶柱色谱、反相ODS柱色谱、制备液相色谱等方法进行分离、纯化,根据化合物的理化性质和光谱数据鉴定其结构。结果 分离得到了7个化合物,经结构鉴定分别为酸浆苦素A（1）、酸浆苦素D（2）、酸浆苦素L（3）、酸浆苦素O（4）、木犀草素（5）、木犀草素-7-O-β-D-葡萄糖苷（6）和胡萝卜苷（7）。结论 共分离得到7个化合物,并对酸浆苦素类化合物1～4进行了较为详细的解析和更为全面的NMR归属。     

印楝化学成分研究

印楝 Azadirachta indica A.Juss.又名印度楝 ,为楝科楝属植物。该植物的种子、树皮和叶的提取物均已进行了研究 ,发现其中含有很多在农业、医药及化妆品方面有经济价值的次生产物 ,如含有多种具杀虫活性的四环三萜类化合物 ,对 2 0 0多种农业害虫有较强的毒杀能力 ,而且对人畜无害 [1 ]。印楝是一种极好的具有杀农虫功效的药用植物。作者对其进行了系统的化学成分研究 ,从其中提取物的正丁醇及水部分分离并鉴定了 7个化合物 ,分别为 :槲皮素 ( ) ,异杨梅树皮苷 ( ) ,β-谷甾醇 ( ) ,豆甾醇 ( ) ,胡萝卜苷 ( ) ,柽柳素 -3 -O-β-D-葡萄糖…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.