免疫球蛋白样物质在上皮来源的恶性肿瘤中的表达

目的研究在上皮来源恶性肿瘤细胞中免疫球蛋白样物质的表达.方法采用免疫组化SP法,分别用鼠抗人IgG、IgD、IgA和IgM单克隆抗体对乳腺癌、肝癌、肺癌、膀胱癌等多种上皮来源恶性肿瘤癌组织及其部分传代细胞进行了检测.结果在上述癌组织及癌细胞中有不同程度的阳性反应,与抗人IgG、IgD、IgA和IgM抗体反应阳性率分别为50.0%、85.7%、61.9%和45.2%.其中乳腺癌阳性率为100.0%.结论在上皮来源恶性肿瘤中存在免疫球蛋白样物质的表达,其性质和机制有待于进一步研究.     

免疫球蛋白样物质在上皮来源的恶性肿瘤中的表达

目的：研究在上皮来源恶性肿瘤细胞中免疫球蛋白样物质的表达。方法：采用免疫组化SP法,分别用鼠抗人IgG、IgA和I gM单克隆体对乳腺癌、肝癌、肺癌、膀胱癌等多种上皮来源恶性肿瘤瘤组织及其部分传代细胞进行了检测。结果：在上述 癌组织及癌细胞中有不同程度的阳性反应,与抗人IgG、IgD、IgA和IgM抗体反应阳性率分别为50.0%、85.7%、61.9%和45.2%。其中乳腺癌阳性率为100.0％ 。结论 ：在上皮来源恶性肿瘤中存在免疫球蛋白样物质 的表达,其性质和机制有待于进一步研究。     

IgE骨髓瘤1例报告及文献复习(英文)

目的报告1例 IgE 骨髓瘤病例并将其临床特征与义献进行比较。方法对1例骨髓瘤患者的血清尿液采用醋酸纤维膜电泳、免疫固定电泳和免疫球蛋白定量等方法鉴定 M 蛋白,采用直接和间接免疫酶标技术检测瘤组织 IgE 及轻链的表达。结果醋酸纤维膜电泳显示住快γ区有一单克隆小峰,占18%.免疫固定电泳结果显示一单克隆区带,与λ抗血清反应,与κ、IgG、IgA、IgM、IgD 抗血清不反应。免疫球蛋白定量结果显示 IgG 21.6 g/L,IgA 1.2 g/L,IgM 2.64 g/L,κ 7.49 g/L,λ16.0 g/L,κ/λ0.47。免疫组织化学染色结果表明瘤组织 IgE 及λ轻链,不表达κ轻链。结论本文所报告的病例为国内首例 IgE 多发性骨髓瘤。     

IgE骨髓瘤1例报告及文献复习

目的 报告1例IgE骨髓瘤病例并将其临床特征与文献进行比较。方法 对1例骨髓瘤患者的血清尿液采用醋酸纤维膜电泳、免疫固定电泳和免疫球蛋白定量等方法鉴定M蛋白,采用直接和间接免疫酶标技术检测瘤组织IgE及轻链的表达。结果 醋酸纤维膜电泳显示在快γ区有一单克隆小峰,占18％。免疫固定电泳结果显示一单克隆区带,与λ抗血清反应,与κ、IgG、IgA、IgM、IgD)抗血清不反应。免疫球蛋白定量结果显示IgG 21.6g/L,IgA 1.2g/L,IgM 2.64g/L,κ 7.49 g/L,λ16.0g/L,κ/λ0.47。免疫组织化学染色结果表明瘤组织IgE及λ轻链,不表达κ轻链。结论 本文所报告的病例为国内首例IgE多发性骨髓瘤。     

2015—2019年某焦化厂下风向居民血清免疫球蛋白分析

目的： 比较2015—2019年某焦化厂下风向居民不同人群血清免疫球蛋白的含量并分析其变化趋势。方法： 2015—2019年夏季，选取中国北部某焦化厂下风向（1~2 km）两个村庄的常住居民为研究群体，按照一定的纳入排除标准确定研究对象，采集其血液及尿液通过全自动生化分析仪分别检测血清免疫球蛋白（IgG、IgA和IgM）含量及尿肌酐（Cr），并对血清免疫球蛋白含量异常率进行统计分析。尿液中多环芳烃代谢物1-羟基芘的含量采用高效液相色谱-荧光法检测。结果： 2015—2019年某焦化厂下风向居民中，女性血清IgG含量均值在各年份间均高于男性（P<0.01），IgM含量均值在2015年和2016年高于男性（P<0.05）；成人血清IgG和IgA含量均值在各年份间均高于未成年人（P<0.01），IgM含量均值在2016年低于未成年人（P<0.01）。2015—2019年某焦化厂下风向居民血清免疫球蛋白含量异常率总体呈先上升后下降的趋势，血清IgA和IgM在不同年份间的差异具有统计学意义（P<0.01），且2016年成人血清IgG、IgA和IgM含量异常率均高于未成年人（P<0.05），2015年和2016年女性血清IgG含量异常率高于男性（P<0.05）。2015—2019年，该地区居民尿液中多环芳烃代谢产物1-羟基芘的检出水平（用尿肌酐含量校正）中位数依次为0.468、0.797、0.678、0.169和0.021 μg/g。结论： 2015—2019年焦化厂下风向居民血清免疫球蛋白（IgG、IgA和IgM）含量具有一定的性别差异和年龄差异，其异常率总体呈现先上升后下降的趋势。     

硼替佐米治疗多发性骨髓瘤导致肠麻痹一例

患者 男,75岁,以外伤后腰痛伴双下肢活动障碍4d于2013年1月22日入院.骨髓穿刺见原幼浆细胞占0.61,提示多发性骨髓瘤（MM）;血清免疫固定电泳发现单克隆免疫球蛋白IgA-κ;CT示腰5椎体骨质破坏,椎管受压,多发胸腰椎密度减低影;PET-CT示全身多发骨质破坏,脱氧葡萄糖（FDG）不同程度摄取增高;血常规示：白细胞计数5.05×109/L,血红蛋白74g/L,红细胞2.67×1012/L,血小板计数303×109/L;肾功能正常;IgA 36.8 g/L,IgG 9.04 g/L,IgM 0.98 g/L,κ-轻链14.4 g/L,λ-轻链1.22 g/L;血β 2微球蛋白8.45 mg/L,清蛋白26g/L.诊断为MM,IgA-κ型,ⅢA期.     

联合应用谷氨酰胺和生长激素对食管癌患者术后蛋白代谢和免疫状况的影响

目的研究谷氨酰胺和重组人生长激素联合应用对食管癌患者术后蛋白代谢、免疫状况的影响。方法将60例食管癌患者随机分为对照组、谷氨酰胺组、谷氨酰胺+生长激素组,三组均于术后给予7 d胃肠外营养,其中谷氨酰胺组按每天0.4 g/kg的剂量加用丙氨酰谷氨酰胺注射液(力肽),谷氨酰胺+生长激素组除了按每天0.4 g/kg的剂量加用谷氨酰胺外,同时还皮下注射8 IU/d重组人生长激素(珍怡),并分别于手术前及手术后第2天、第6天测量血清白蛋白水平,T细胞亚群、IgA、IgG、IgM,并与对照组加以比较。结果谷氨酰胺组的血清白蛋白、IgA、IgG在术后第6天分别为(42.8±3.36)g/L、(2.74±2.2)g/L和(10.8±2.2)g/L,较对照组有显著的改善。谷氨酰胺+生长激素组的血清白蛋白、IgA、IgG在术后第6天分别为(48.74±3.24)g/L、(3.12±1.7)g/L和(13.03±1.7)g/L,较对照组及谷氨酰胺组均有显著的改善。但IgM及T细胞亚群改变不显著。结论食管癌患者在术后常规TPN治疗的基础上,联合应用谷氨酰胺加重组人生长激素可以更好地改善患者术后的蛋白代谢及体液免疫水平。     

肿瘤患者放、化疗后外周血淋巴细胞损伤和免疫功能改变的研究

目的观察肿瘤患者放、化疗后外周血淋巴细胞损伤和免疫功能改变,并分析两者之间的关系.方法30例肿瘤患者分成放射治疗组(放疗组)和化学药物治疗组(化疗组),分别在放疗前后,化疗前后测定外周血双核淋巴细胞微核率(‰)、T淋巴细胞转化率(%)及血清免疫球蛋白(IgG,IgA,IgM),并进行对比分析.双核淋巴细胞微核率(MNR)用细胞松驰素B(CB)法测定,T淋巴细胞转化率(TLTR)及免疫球蛋白(IgG,IgA,IgM),均用常规方法测定.结果放疗组和化疗组在放、化疗后外周血双核淋巴细胞微核率显著性升高,而T淋巴细胞转化率和血清免疫球蛋白(IgG,IgA,IgM)均显著性降低.结论放、化疗后,两组患者的外周血双核淋巴细胞微核率(‰)与T淋巴细胞转化率(%)呈负相关关系.     

肝癌患者74例免疫球蛋白、补体水平及AFP分析

目的　探讨肝癌患者体液免疫指标血清球蛋白、IgG、IgA、IgM及补体 3 (C3 )、补体 4(C4)的变化及其与AFP的关系。方法　用自动生化分析仪和放免法定量测定肝癌患者的血清球蛋白、IgG、IgA、IgM、C3、C4及AFP的含量 ,设肝良性疾病肝硬化和正常人两组为对照组 ,运用SPSS软件进行统计。结果　肝癌和肝硬化组的球蛋白、IgG、IgA比正常人明显升高 (P <0 .0 1) ;肝癌组C3比正常人低 (P <0 .0 1) ,C4则高于正常人 (P <0 .0 1) ;AFP阳性和AFP阴性肝癌患者的免疫球蛋白、补体均无差异 (P >0 .0 5 ) ;肝癌患者的AFP与免疫球蛋白、补体无相关性。结论　肝癌患者的体液免疫功能增强 ,AFP与免疫球蛋白、补体无相关性。     

血清单克隆免疫球蛋白在慢性B淋巴细胞增殖性疾病中的表达及其意义

目的 研究血清单克隆免疫球蛋白(McIg)在慢性B淋巴细胞增殖性疾病(B-CLPD)中的表达情况,分析其临床意义及可能来源.方法 回顾性分析2006年5月至2015年5月1 147例初诊B-CLPD患者临床资料,分析血清McIg表达情况及预后相关因素,探讨其来源.结果 1 147例B-CLPD患者中淋巴浆细胞淋巴瘤/华氏巨球蛋白血症(LPL/WM)患者164例,其中140例(85.4％)存在McIg;非LPL/WM患者983例,其中50例(5.1％)存在McIg;两组McIg类型均以IgM型为主.McIg阳性LPL/WM组、McIg阳性的非LPL/WM组及McIg阴性组患者血清IgM浓度分别为(48.88±33.42)g/L、(27.9±15.23)g/L、(2.75±1.21)g/L,差异有统计学意义(P=0.000),McIg阳性的LPL/WM组IgM浓度高于McIg阳性非LPL/WM组(P=0.000),且两组患者血清IgM浓度均高于McIg阴性者(P值均为0.000).分析16例McIg阳性非LPL/WM的B-CLPD患者显示,治疗后完全缓解期间单克隆IgG、Igi的水平变化均低于初诊时(P值分别为0.001、0.048).非LPL/WM的B-CLPD患者中β 2微球蛋白高水平组及染色体复杂核型组血清McIg阳性率分别高于B2微球蛋白低水平组(P=0.001)及染色体正常核型组(P=0.016).47例McIg阳性的非LPL/WM患者中,38例(80.9％)血清McIg表达类型与肿瘤细胞表面Ig轻链的类型一致,二者中度相关(P＜ 0.005).结论 部分非LPL/WM的B-CLPD患者血清中也存在McIg,且可能和预后有一定相关性.McIg可能是由肿瘤细胞或与肿瘤细胞有共同前体的细胞分泌的.     

Alterations in IgG subclasses in acquired immune deficiency syndrome

Decreased IgG subclass levels in pyogenic infections and immunocompromised situations have been described. A study was made to determine IgG subclass levels in four groups of 68 Hispanic patients. The first group consisted of 25 terminal patients with AIDS, the second group of 20 i.v. drug abusers, and the third group of eight hospital patients with neither a diagnosis of AIDS/ARC nor a history of i.v. drug abuse. IgG subclass levels of these 53 cases were compared with those of a fourth group of 15 normal controls. The total IgG, IgA, and IgM levels as well as the four IgG subclass concentrations were measured by radial immunodiffusion using appropriate standards and specific antisera. The first two groups had similar values, with an average IgG level of 10.37 g/liter; IgA, 2.68; and IgM, 1.78; subclass levels were IgG1, 6.68 g/liter; IgG2, 2.77; IgG3, 0.34; and IgG4, 0.68. These were significantly lower than those of controls, except for IgG4. Determination of minor subclasses may offer some possibilities for immunomodulation and therapy and could be useful in terms of prognosis.     

80例肺癌患者免疫球蛋白、补体及CEA分析

目的 :研究肺癌患者体液免疫指标的变化及其与CEA的关系。方法 :用自动生化分析仪和放免法定量测定 80例肺癌患者的血清球蛋白、IgG、IgA、IgM及补体 3(C3)、补体 4 (C4 )的量 ,设肺炎和正常人两组为对照组 ,运用SPSS软件进行统计。结果 :肺癌组的球蛋白、IgG、IgA比肺炎和正常人明显升高(P <0 0 5) ,C3则比正常人降低 (P <0 0 5) ,IgM、C4 两组无差异 (P >0 0 5) ,CEA阳性和CEA阴性肺癌患者免疫球蛋白、补体均无差异 (P >0 0 5) ,肺癌患者的血清CEA与IgA浓度呈显著正相关 (P <0 0 1)。结论 :肺癌患者的体液免疫功能增强 ;CEA的产生可能是促进IgA分泌增多的原因之一     

80例肺癌患者免疫球蛋白,补体及CEA分析

目的：研究肺癌患者体液免疫指标的变化及其与ＣＥＡ的关系。方法：用自动生化仪和放免法定量测定８０例肺癌患者的血清球蛋白、ＩｇＧ、ＩｇＡ、ＩｇＭ及补体３（Ｃ３）,补体（Ｃ４）的量,设肺部良性疾病肺炎和正常人两组为对照组,运用ＳＰＳＳ软件进行统计。结果：肺癌组的球蛋白、ＩｇＧ、ＩｇＡ比肺炎和正常人明显升高,Ｃ２则比正常人降低,ＩｇＭ、Ｃ４两组差异无显著性,ＣＥＡ阳性和ＣＥＡ阴性肺癌患者免疫球蛋白、则比正     

Local immunoglobulin production in breast cancer

Immunoglobulin levels (IgA, IgG, IgM) have been estimated in protein extracts of 55 malignant and 20 benign tumours of the breast, and in 17 normal tissues from a cancer bearing breast. IgA and IgG were significantly reduced in cancer compared with both benign and normal tissues but IgM, detected in only a third of tumours, was significantly increased. Total immunoglobulin levels and IgG in the malignant tumours correlated with plasma cell infiltration.     

Immunoglobulin-producing cells in the "transitional" mucosa adjacent to adenocarcinomas of the human large bowel.

Quantification of IgA-, IgM-and IgG-producing immunocytes was performed in a defined mucosal tissue unit adjacent to 16 adenocarcinomas of the large bowel, and compared with data for control units from 15 histologically normal specimens. The thickness of the “transitional” mucosa adjacent to the tumours was markedly increased. Thus, the average cancer-associated unit showed a 2.6-fold increase in height and its number of immunoglobulin-producing cells was raised 2.8 times. The numerical increase for IgA cells was 2.1, for IgM cells 5.1, and for IgG cells 13.0; the percentage proportions of these cell classes were changed from 91:6:3.6 to 71:12:17. Only very rare IgD-and IgE-producing cells were seen. The immunocyte density in 6-μm-thick sections (cell number/mm² of lamina propria) was 823, 136 and 201 for the IgA, IgM and IgG class, respectively, compared to the normal figures of 1,186, 73 and 49. The density of IgG and IgM cells was thus significantly increased and that of IgA cells decreased in the lamina propria of the “transitional” mucosa. The intra-individual scatter of all variables, including mucosal height, was large in the “transitional” mucosa, but the variables became normalized approximately 1 cm from the tumour. The excessive rise in the local production of IgG, with no concomitant increase in the density of IgA cells, indicates that there are local stimulatory factors bypassing the secretory IgA immune system. Further studies are needed to show if this local immune response reflects production of tumour-specific antibodies or is merely due to increased penetrability for antigens from the gut lumen passing through the tumour lesion.     

Excess IgD bearing lymphocytes in patients with malignant melanoma

B lymphocytes from the peripheral blood of 15 melanoma patients and 14 normal adults were studied using immunofluorescence to IgA, IgG, IgD and IgM surface markers. The total number of peripheral blood B lymphocytes was increased in the melanoma patients (1048/mm³) compared with normal controls (504) (P<0·01).     

Heterogenous Enzyme Immunoassay: Detection of Anti-Candida albicans specific Ig-Isotypes and Mannan Antigen/Heterogener Enzym-Immunoassay: Nachweis von Anti-Candida albicans spezifischen Ig-Isotypen und Mannan-Antigen

Summary: The serum levels of anti-Candida albicans IgA, IgG and IgM class antibodies were quantified by heterogenous sandwich enzyme-immunoassay. The presence of polysaccharide antigen reactive with rabbit antiserum to Candida albicans was also demonstrated by this assay. The thermal dissociation of antigen-antibody complex was proved to be efficient in quantitative determination of fungal antigens. Using the methods of correlation analysis, the close relationship between Ig-isotypes was studied. The statistically significant correlation between IgG and IgA class (r_x,y=0.475), and IgA and IgM class (r_x,y=0.465) was revealed. The correlation between IgG and IgM class (r_x,y=0375) for α=0.01 was decreased significantly.
Zusammenfassung: Die Serumspiegel der Anti-Candida albicans Antikörper der Klassen IgA, IgG und IgM wurden durch den heterogenen Sandwich-Enzyminununoassay quantifiziert. Die Anwesenheit von Polysaccharidantigen, das mit Kaninchenantiserum gegen Candida albicans reagiert, wurde ebenfalls durch diesen Assay dargestellt. Die Hitze-Dissoziation des Antigen-Antikörperkomplexes envies sich bei der quantitativen Bestimmung des Pilzantigens als wirksam. Unter Verwendung der Methoden der Korrelationsanalyse wurde die enge Beziehung zwischen Ig-Isotypen studiert. Es zeigte sich eine statistisch significante Korrelation zwischen IgG und IgA Klasse (r_x,y=0.475) und IgA und IgM Klasse (r_x,y=0.465). Die Korrelation zwischen IgG und IgM Klasse war signifikant abnehmend (r_x,y=0.375) auf α=0.01.     

Immunoglobulin synthesis and secretion by leukemic B cells from patients with chronic lymphocytic leukemia

We investigated the ability of purified E-rosette negative largely leukemic B cells from 15 patients with B-cell chronic lymphocytic leukemia (CLL) to synthesize and secrete IgM, IgA and IgG spontaneously or in the presence of purified autologous or allogeneic T4 cells from normal donors, in PWM-induced differentiation system. We observed moderate but significant IgM synthesis and secretion (19.7 +/- 8.9 micrograms/dl, n = 5) by leukemic B cells alone in 5 of 15 patients examined. These IgM concentrations were significantly higher (p less than 0.005) than those produced by purified E-rosette negative cells from normal donors (4.3 +/- 4.5 micrograms/dl; n = 6) in the absence of T cells. Purified E-rosette negative leukemic B cells alone from patients with CLL did not produce IgA or IgG. Addition of purified autologous or allogeneic T4 cells from normal donors resulted in significant increase of IgM production by leukemic B cells from certain patients or initiated IgM secretion in others. However, these IgM levels (73.9 +/- 56.6 micrograms/dl) were significantly lower (p less than 0.003) to those produced by mixtures of T4 cells and B cells form normal donors (211.6 +/- 58.0 micrograms/dl, n = 6). Addition of purified autologous or allogeneic T4 cells from normal donors to purified largely leukemic B cells from patients with CLL resulted in production of very small amounts of IgA in 4 of 15 patients (10.6 +/- 6.3 micrograms/dl vs 154.7 +/- 35.8 micrograms/dl produced by T4 and B cells from normal donors; n = 6), but did not support IgG synthesis and secretion. Purified T4 cells from certain patients with CLL exhibit defective helper function to immunoglobulin production by E-rosette negative cells from normal donors.     

80例肺癌患者的免疫球蛋白、补体水平及其与CEA的关系

目的：研究肺癌患者体液免疫指标血清球蛋白、ＩｇＧ、ＩｇＡ、ＩｇＭ及补体３（Ｃ３）、补体４（ＣＶ４）的变化及其与癌胚抗原（ＣＥＡ）的关系。方法：用自动生化分析仪和放免法定量测定８０例肺癌患者的血清球蛋白、ＩｇＧ、ＩｇＡ、ＩｇＭ及Ｃ３、Ｃ４及ＣＥＡ的含量,肺部良性疾病肺炎和正常人为两对照组,运用ＳＰＳＳ软件进行统计。结果：肺癌组的球蛋白、ＩｇＧ、ＩｇＡ比肺炎组和正常人显著升高（Ｐ〈０．０１和Ｐ〈０．０