Increased interleukin-6 in aqueous humor of neovascular glaucoma.

PURPOSE: To demonstrate the involvement of proinflammatory cytokines in intraocular neovascularization by detecting the presence of interleukin (IL)-6, IL-2, and tumor necrosis factor (TNF)-alpha in aqueous humor and serum of patients with neovascular glaucoma (NVG) secondary to central retinal vein occlusion (CRVO). METHODS: According to the grade of iris neovascularization (NVI), patients with CRVO were divided into three groups: CRVO without NVI, CRVO with NVI, and CRVO with regressed NVI. Healthy patients with cataract were enrolled as control subjects. Enzyme-linked immunosorbent assay was used to quantitate the concentrations of the cytokines IL-6, IL-2, and TNF-alpha in aqueous humor and serum from patients with NVG and control subjects. RESULTS: In serum, the levels of IL-6, IL-2, and TNF-alpha did not differ among groups. In aqueous humor, only IL-6 showed significant change among groups. IL-6 levels in aqueous humor of group 2, CRVO with NVI (1532.0+/-221.1 pg/ml; P < 0.001), and group 3, CRVO with regressed NVI (234+/-154.6 pg/ml; P < 0.001), were significantly higher. There was no significant difference in IL-6 levels between the control group (26.4+/-21.8 pg/ml) and group 1 (15.6+/-0.9 pg/ml). CONCLUSIONS: The inflammatory cytokine IL-6 in aqueous humor increased spatially and temporally correlated with the grade of NVI in patients of NVG secondary to CRVO. The aqueous IL-6 increased in NVI and decreased after vessels regressed. It is possible that the significantly higher level of IL-6 was due to intraocular synthesis because of the minimal change in serum. The increased level of IL-6 may have a putative role along with other angiogenic factors in angiogenesis of NVG as a possible predictor of NVI.     

新生血管性青光眼患者房水中血小板反应蛋白－1的表达及其与血管内皮生长因子相关关系的临床研究

目的检测新生血管性青光眼(NVG)与年龄相关性白内障患者房水中血小板反应蛋白－1(TSP－1)的表达情况,并分析NVG患者房水中的TSP－1与血管内皮生长因子(VEGF)的关系。 方法纳入30例(30只眼)NVG患者(NVG患者组)以及30例(30只眼)年龄相关性白内障患者(对照组)。所有NVG患者在行抗青光眼手术前抽取房水,同时在白内障手术时抽取年龄相关性白内障患者房水。采用酶联免疫吸附试验检测房水以及血液中TSP－1与VEGF的浓度。房水和血液中VEGF浓度和TSP－1浓度采用均数±标准差( ±s)表示,采用独立样本t检验进行组间比较。各细胞因子之间、细胞因子浓度与年龄、眼压之间的相关性用采用Spearman's相关分析。 结果NVG组患者房水中TSP－1浓度为(15.47±2.28) ng/ml,而对照组患者房水中TSP－1浓度为(2.28±1.59)ng/ml,NVG组患者房水中TSP－1浓度明显高于对照组,差异有统计学意义(t＝12.70,P<0.05)。NVG组患者与对照组患者房水中VEGF浓度分别为(3355.81±2284.54)pg/ml及(262.68±125.66 )pg/ml,差异有统计学意义(t＝7.41,P<0.05)。NVG组患者血浆中TSP－1浓度为(0.65±0.27)ng/ml,对照组患者血浆中TSP－1浓度为(0.57±0.25)ng/ml,两组之间浓度相当,差异无统计学意义(t＝1.16,P>0.05)。NVG组患者的VEGF血浆浓度为(13.01±7.60)pg/ml,对照组患者的VEGF血浆浓度为(14.62±6.96)pg/ml。二者比较,差异无统计学意义(t＝－0.86,P>0.05)。NVG患者房水中TSP－1与VEGF浓度呈显著正相关(r＝0.479,P<0.05)。在NVG组中,不管是TSP－1因子还是VEGF,其房水中的浓度与血浆中的浓度均无显著相关性。同样,在对照组中,房水TSP－1浓度与血浆TSP－1浓度无相关性,且房水VEGF浓度与血浆VEGF浓度亦无相关性。 结论NVG患者房水中TSP－1与VEGF表达明显上调,明显上调的TSP－1与VEGF呈现显著正相关。     

新生血管性青光眼患者房水中信号素3A表达水平的研究

目的　检测新生血管性青光眼（ｎｏｎｖａｓｃｕｌａｒｇｌａｕｃｏｍａ,ＮＶＧ）患者房水中信号素３Ａ（Ｓｅｍａｐｈｏｒｉｎ３Ａ,Ｓｅｍａ３Ａ）的水平,探讨Ｓｅｍａ３Ａ在ＮＶＧ发生发展中的作用。方法　选取在我院眼科２０１４年１月至２０１５年２月诊断为ＮＶＧ行抗青光眼手术患者４２例（４３眼）作为ＮＶＧ组,并根据病因分为糖尿病组１１眼、静脉阻塞组１１眼、青光眼组８眼及原因不明组１３眼;选取同期收治行单纯白内障手术患者１６例（１６眼）作为对照组。分别于术前抽取房水样本,利用双抗体夹心酶联免疫吸附试验测定房水中Ｓｅｍａ３Ａ含量,对比分析ＮＶＧ组和对照组在Ｓｅｍａ３Ａ浓度上的统计学差异。分析检测ＮＶＧ组房水中Ｓｅｍａ３Ａ水平与眼压水平的相关性。结果　ＮＶＧ组患者的眼压为（３２．６３±１１．１６）ｍｍＨｇ（１ｋＰａ＝７．５ｍｍＨｇ）,较对照组（１５．５６±１．９３）ｍｍＨｇ明显升高（ｔ＝２３．９６,Ｐ＜０．０００１）。ＮＶＧ组患者房水中Ｓｅｍａ３Ａ浓度为（２．１３±１．９４）ｎｇ?ｍＬ－１,明显高于对照组（０．６６±０．５８）ｎｇ?ｍＬ－１,差异有显著统计学意义（ｔ＝２．９８５,Ｐ＜０．０００１）。但ＮＶＧ组房水中Ｓｅｍａ３Ａ与眼压水平无明显相关性（ｒ＝－０．２１６４,Ｐ＝０．１１８５）。根据引起ＮＶＧ的原发病进行分组比较,糖尿病组Ｓｅｍａ３Ａ浓度为（２．１４±１．４４）ｎｇ? ｍＬ－１、静脉阻塞组Ｓｅｍａ３Ａ浓度为（１．６７±０．９８）ｎｇ?ｍＬ－１及青光眼组Ｓｅｍａ３Ａ浓度为（１．８７±１．８０）ｎｇ?ｍＬ－１,与对照组房水中Ｓｅｍａ３Ａ浓度相比均明显升高,差异均有统计学意义（Ｐ＝０．００１、０．００２、０．０２１）。结论　Ｓｅｍａ３Ａ在ＮＶＧ患者房水中明显升高,推测其表达增加可能参与了ＮＶＧ患者虹膜新生血管形成及眼部损伤性改变。     

NVG患者房水和血清中VEGF-A和PDGF及PEDF的临床意义

目的：检测新生血管性青光眼(NVG)患者房水和血清中血管内皮生长因子-A(VEGF-A)、血小板源性生长因子(PDGF)、色素上皮衍生因子(PEDF)的含量,并分析其意义。

方法：前瞻性临床研究。采用酶联免疫吸附法(ELISA)检测2015-12/2016-12在西京医院眼科住院的NVG患者23例及年龄相关性白内障患者23例房水及血清中VEGF-A、PDGF、PEDF的含量并定量分析。

结果：NVG患者房水中VEGF-A、PDGF的含量分别为1130.56±69.32、221.95±56.08ng/L,均显著高于年龄相关性白内障患者组(226.45±37.46、36.25±7.12ng/L)(均P<0.01); NVG患者房水中PEDF的含量为195.69±42.00ng/L,低于年龄相关性白内障患者组(497.89±12.52ng/L)(P<0.01); NVG组血清中VEGF-A、PDGF及PEDF的含量分别为226.45±37.46、29.57±6.31及13.24±1.76ng/L,均与年龄相关性白内障患者组(219±34.89、28.28±7.24、12.96±2.08ng/L)无差异(均P>0.05)。NVG患者房水中VEGF-A和PDGF含量呈正相关(r=0.502,P=0.015),而VEGF-A与PEDF含量呈负相关(r=-0.480,P=0.020)。

结论：NVG患者房水中VEGF-A、PDGF的含量显著升高,而PEDF的含量显著降低,且VEGF-A的含量与PDGF含量呈正相关、与PEDF含量呈负相关。联合使用VEGF-A、PDGF抑制剂及PEDF可能为NVG治疗提供新的思路。     

The lens influences aqueous humor levels of transforming growth factor-β2

Background: Transforming growth factor-beta 2 (TGF-β2) is a pluripotent cytokine which has been suggested to play a number of roles in ocular physiologic and pathologic states. Intraocular fluid (IOF) levels of TGF-β2 are quite high. Although the sources of ocular TGF-β are not completely defined, the retinal pigment epithelium, the epithelium of the ciliary body and trabecular meshwork cells all secrete it. In this study we utilized canine lens and rabbit ciliary pigmented epithelial cell cultures to quantitate the in vitro secretion of TGF-β2. In addition, the effects of aphakia or the presence of cataractous lenses on IOF TGF-β2 levels were determined. · Methods: Lens and ciliary body epithelial cell culture supernatants and aqueous humors were assayed for total TGF-β2 levels by ELISA and bioassay. · Results: TGF-β2 accumulated in the media bathing lens epithelial cell cultures (0.7 ± 0.03 ng/ml at day 2) and ciliary pigmented epithelial cell cultures (0.8 ± 0.06 ng/ml at day 2) in a time-dependent manner. Surprisingly, aqueous humor from aphakic rabbit eyes contained significantly higher levels of TGF-β2 than their contralateral phakic controls. Furthermore, aqueous humor from canine eyes with cataracts also contained significantly higher levels of TGF-β2 than normal eyes. · Conclusions: These results suggest that the lens secretes TGF-β2 and that the presence and status of the lens may influence IOF TGF-β2 levels. Received: 12 August 1996 Revised version received: 18 June 1997 Accepted: 18 July 1997     

Elevated homocysteine levels in aqueous humor of patients with pseudoexfoliation glaucoma

PURPOSE: To determine total homocysteine levels in aqueous humor of pseudoexfoliation open-angle glaucoma patients. DESIGN: Case-control study. METHODS: Total homocysteine levels were measured by enzyme-linked immunosorbent assay in aqueous humor and plasma of 29 patients with pseudoexfoliation glaucoma and 31 control patients with cataract. Patients with factors affecting homocysteine levels were excluded. RESULTS: We observed significantly elevated (twofold) homocysteine levels in the aqueous humor of the glaucoma patients (Z = -5.11, P <.0001). Additionally, the calculated ratio (plasma:aqueous humor) was significantly lower in these patients (Z = -3.57, P <.001), and aqueous homocysteine was significantly correlated with their respective elevated plasma levels (r =.42, P =.02). CONCLUSIONS: Because homocysteine induces vascular injury and alterations of extracellular matrix, high aqueous homocysteine may trigger the abnormal matrix accumulation characteristic. It may reflect the proposed impairment of the blood-aqueous barrier of pseudoexfoliation open-angle glaucoma and be involved in its pathogenesis.     

Increased levels of vascular endothelial growth factor in the aqueous humor of patients with diabetic retinopathy

Purpose:

This study aims to investigate the levels of aqueous vascular endothelial growth factor (VEGF) in diabetic patient groups in comparison to normal subjects, and to correlate elevated VEGF with the severity of diabetic retinopathy (DR).

Materials and Methods:

Aqueous samples were obtained from 78 eyes of 74 patients undergoing intraocular surgery and they were examined by the enzyme-linked immunosorbent assay. Color photographs, optical coherence tomography scans, and fluorescein angiography were used to evaluate patients preoperatively.

Results:

A strong statistical correlation was found to exist between the level of aqueous VEGF and the severity of DR (P < 0.001), whereas, the VEGF levels in a control group and a diabetic group without DR were not significantly different (P = 0.985). Aqueous VEGF levels were significantly elevated in patients with proliferative DR (PDR) as compared to the control group (P < 0.001), to diabetic patients without retinopathy (NDR) (P < 0.001), and to diabetic patients with nonproliferative DR (NPDR) (P < 0.001). The aqueous VEGF levels were significantly higher in patients with active PDR than in those with quiescent PDR (P = 0.001). On the other hand, a statistically insignificant (P = 0.065) correlation was found between elevated aqueous VEGF and the presence of macular edema in the NPDR group.

Conclusions:

VEGF was elevated in the aqueous humor of patients with DR compared to that in normal eyes. The aqueous VEGF level had a strong correlation with the severity of retinopathy along with a statistically insignificant difference in macular edema.     

Increased aqueous humor basic fibroblast growth factor and hyaluronan levels in relation to the exfoliation syndrome and exfoliative glaucoma.

PURPOSE: To quantify the concentrations of basic fibroblast growth factor (bFGF) and hyaluronan (HA) in the aqueous humor of patients with the exfoliation syndrome (XFS) or exfoliative glaucoma (XFG). METHODS: Aqueous humor bFGF and HA levels were measured in 13 patients with XFS and in 7 patients with XFG. The results were compared with those obtained from 17 healthy controls. RESULTS: Mean bFGF levels were significantly higher in the XFG patients than those in the XFS patients, which in turn were higher than the bFGF levels in the healthy individuals. Aqueous humor HA levels in both patients with the XFS and the XFG were significantly higher compared to the controls. CONCLUSION: We suggest that bFGF plays an important role in the pathogenesis of XFS and XFG, as well as in the synthesis of secreted HA, which may result in connective tissue degradation that affects the ocular anterior segment.     

Increased vascular endothelial growth factor levels in aqueous humor and serum of patients with quiescent uveitis

PURPOSE: Vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) are angiogenic mediators that share a significant proinflammatory activity. Both substances have been suggested to play a key role in uveitis pathogenesis. The authors analyzed VEGF and IL-8 levels in the aqueous humor and serum of patients with different types of uveitis during a quiet phase of the disease. METHODS: Thirteen patients with intermediate uveitis, uveitis associated with ankylosing spondylitis, Vogt-Koyanagi-Harada disease, Fuchs uveitis syndrome, idiopathic chronic anterior uveitis, or Behcet disease, as well as 10 normal matched subjects, were included in the study. VEGF and IL-8 concentrations were measured in aqueous humor and serum by enzyme-linked immunosorbent assay. RESULTS: VEGF levels were significantly higher in both the aqueous humor and serum of patients with uveitis as compared with controls. IL-8 concentrations in aqueous humor were significantly higher in patients with uveitis with extraocular manifestations than in those with eye-limited disease. CONCLUSIONS: These findings suggest that VEGF plays a role in uveitis pathogenesis even during inactive disease and that IL-8 levels are significantly influenced by the presence of uveitis-associated extraocular changes.     

新生血管性青光眼患者房水中血小板源性生长因子-C和血管内皮生长因子水平的测定和分析

背景 新生血管性青光眼(NVG)是由不同病因引起的严重致盲眼病,与视网膜组织缺氧后分泌因子有关,如血管内皮生长因子(VEGF)等,但仅用抗VEGF疗法并不能完全抑制新生血管的生长.我们先前的研究发现,血小板源性生长因子-C(PDGF-C)参与眼内病理性新生血管的生成,但PDGF-C在NVG中的作用尚不清楚. 目的 定量检测NVG患者房水中VEGF和PDGF-C的质量浓度,为NVG的治疗提供新的思路. 方法 采用前瞻性队列研究方法,收集2014年1月至2015年8月在第四军医大学西京医院就诊的NVG患者62例62眼,其中10眼近1年内曾行视网膜光凝和/或冷凝治疗,其他52眼中原发病为视网膜中央静脉阻塞(CRVO)者16眼,糖尿病视网膜病变(DR)者20眼,视网膜脱离(RD)术后者5眼,视网膜血管炎(Eales病)者4眼,未知原因者7眼;虹膜新生血管Ⅱ级者13眼,Ⅲ级者29眼,Ⅳ级者10眼.同期纳入年龄相关性白内障患者11例11眼作为对照.分别于手术中采集受检眼房水0.1 ～0.2 ml,应用ELISA法检测受检眼房水中VEGF和PDGF-C质量浓度. 结果 NVG组患眼房水中VEGF和PDGF-C质量浓度分别为(1 138.17±69.31)ng/L和(29.80±1.64) ng/L,明显高于对照组的(679.54±49.81)ng/L和(18.60± 1.85) ng/L,差异均有统计学意义(t=20.95、20.49,均P＜0.01).视网膜光凝和/或冷凝组患眼房水中VEGF和PDGF-C质量浓度分别为(1 095.99±52.71) ng/L和(28.55±0.94) ng/L,均低于非光凝和/或冷凝组的(1 146.28±69.57) ng/L和(30.04±1.64) ng/L,差异均有统计学意义(t=-2.160,P=0.034;t=-2.760,P=0.008).NVG患者房水中VEGF与PDGF-C质量浓度呈正相关(r=0.346,P=0.006).不同原发病组间及不同虹膜新生血管分级组间患眼房水中VEGF和PDGF-C质量浓度的总体比较差异均无统计学意义(均P＞0.05). 结论 NVG患者房水中VEGF和PDGF-C质量浓度明显升高,视网膜光凝和/或冷凝治疗可抑制VEGF和PDGF-C的产生,靶向抑制PDGF-C可为NVG的抗新生血管治疗提供新的选择.     

Concentration of transforming growth factor beta2 in aqueous humor

The total transforming growth factor (TGF) beta(2) concentration in the anterior chamber aqueous humor of 96 cataract patients with ages ranging from 17 to 88 years was measured using ELISA to investigate the changes that occur with age, difference of axial length, difference of localization of opacification of the cataractous lens and complications with other eye diseases. It was found that the total TGF-beta(2) concentration (1) decreases with age, (2) shows slight changes with axial length, (3) has slight changes with difference of localization of opacification, (4) is significantly high in patients with concurrent open-angle glaucoma (p < 0.05), (5) is high in patients with complicating diabetes who have undergone panretinal photocoagulation for diabetic retinopathy (p < 0.05) and (6) is low in patients with atopic cataracts. There have been several reports on point 4 above, but none to date of the other points. These findings provide useful information on the intraocular activity of TGF-beta(2).     

Endothelin 1 levels in the aqueous humor of dogs with glaucoma

PURPOSE: Endothelin 1 is a small peptide that is involved in regulation of intraocular pressure and modulation of ocular circulation. To investigate the role of endothelin 1 in canine glaucoma, the authors measured aqueous humor levels of endothelin 1 in healthy dogs and in dogs with hypertensive glaucoma. METHODS: Aqueous humor samples were obtained with general anesthesia from the eyes of healthy dogs (n = 5) and dogs with hypertensive glaucoma (n = 10). Measurements were made by enzyme immunoassay for endothelin 1. RESULTS: The endothelin 1 aqueous humor range was 1.12 - 3.63 pg/mL for healthy dogs and 1.97 - 14.97 pg/mL for glaucomatous dogs. The healthy and glaucomatous canine endothelin 1 aqueous levels (mean +/- SD) were 2.33 +/- 0.90 and 8.11 +/- 5.03 pg/mL, respectively. A two-way analysis of variance indicated that this difference was significant (P = 0.0084). The effect of age on endothelin 1 levels was not significant (P = 0.6283). The large variability found within the glaucomatous group could be explained by the degree of damage of the retina (P = 0.0006). There was no significant correlation between intraocular pressure and endothelin 1 aqueous humor levels within the glaucomatous group (P = 0.29). CONCLUSIONS: The aqueous humor of dogs with hypertensive glaucoma contains significantly higher levels of endothelin 1 than that of healthy dogs.     

Increased melatonin levels in aqueous humor of patients with proliferative retinopathy in type 2 diabetes mellitus

AIM: To report the association between melatonin levels in aqueous humor and serum, and diabetic retinopathy (DR) grade in type 2 diabetic patients. METHODS: Aqueous humor and plasma samples from 26 patients with DR (in nonproliferative and proliferative stages) and 14 control subjects were collected during cataract surgery after 6 p.m. Melatonin concentrations were determined using an enzyme-linked immunosorbent assay (ELISA). RESULTS: Melatonin levels were significantly higher in the aqueous humor of patients with proliferative diabetic retinopathy (PDR) [18.57±2.67 pg/mL (range 15.20-23.06) vs 13.63±2.71 pg/mL (range 10.20-20.20), P=0.0001], but not in those with nonproliferative retinopathy (NPDR) [13.79±2.56 pg/mL (range 9.80-20.10) vs 13.63±2.71 pg/mL (range 10.20-20.20), P=0.961] compared to controls. There was decrement in the plasma melatonin level of patients with PDR, but no significant differences between the plasma melatonin levels of the study groups [5.37±1.74 pg/mL (range 2.85-8.65) vs 6.11±1.90 pg/mL (range 3.13-9.41), P=0.293], or between control and DR groups [NPDR 6.11±1.90 pg/mL (range 3.13-9.41) vs control 6.15±1.91 pg/mL (range 2.18-9.86); PDR (5.37±1.74 pg/mL (range 2.85-8.65) vs control 6.15±1.91 pg/mL (range 2.18-9.86), P=0.808, P=0.264]. CONCLUSION: Elevated melatonin levels in aqueous humor in PDR may indicate the level to be associated with DR severity.     

Increased levels of pigment epithelium-derived factor in aqueous humor of patients with uveitis

AIM: To evaluate whether aqueous humor levels of pigment epithelium-derived factor (PEDF) are increased in patients with uveitis METHODS: Aqueous humor levels of PEDF and tumour necrosis factor alpha (TNFalpha) were determined by ELISA in 34 uveitis samples and 9 cataract control samples. RESULTS: Aqueous humor PEDF and TNFalpha levels were significantly higher in patients with uveitis than in controls (mean (SD) 6.4 (0.8) v 1.3 (0.2) microg/ml and 14.7 (3.8) v 4.2 (0.4) pg/ml, respectively; p<0.01). A positive correlation between PEDF and TNFalpha was found in patients with uveitis (r = 0.40, p<0.01). Furthermore, PEDF levels in aqueous humor were increased in proportion to the disease activity of uveitis. CONCLUSION: The results show that aqueous humor levels of PEDF are increased in patients with uveitis. Our observations suggest that aqueous humor levels of PEDF may be increased as a countersystem against inflammation in uveitis.     

血清及房水中VEGF、IL-6水平与新生血管性青光眼的相关性研究

目的测定新生血管性青光眼（NVG）患者血清及房水中血管内皮生长因子（VEGF）、白细胞介素-6（IL-6）的水平,探讨VEGF、IL-6在NVG发生发展中的作用。方法选取NVG患者20例（20只眼）作为实验组（A组）,原发性慢性闭角型青光眼患者（B组）、老年性白内障患者（C组）各20例（20只眼）作为对照组。采集3组患者血清及房水标本,通过双抗体夹心酶联免疫吸附试验（ELISA法）分别检测血清与房水中VEGF、IL-6的水平。结果 （1）A组房水中VEGF、IL-6的水平（1336.80±70.15）pg/ml、（691.15±50.09）pg/ml明显高于B组（311.60±31.06）pg/ml、（168.25±11.95）pg/ml和C组（165.75±13.95）pg/ml,（92.10±9.59）pg/ml,3组间两两比较,差异均具有统计学意义（F=4019.334,P〈0.01;F=2275.019,P〈0.01）。A组血清中VEGF、IL-6的水平（545.40±155.49）pg/ml、（291.35±22.66）pg/ml高于B组（321.15±52.57）pg/ml、（104.35±13.21）pg/ml和C组（176.30±20.38）pg/ml、（87.00±12.70）pg/ml,3组间两两比较,差异均具有统计学意义（F=75.940,P〈0.01;F=906.947,P〈0.01）。（2）A组房水中VEGF与IL-6的水平呈显著的正相关性,差异具有统计学意义（r=0.857,P〈0.01）。其余各组标本中无显著的相关性（P〉0.05）。结论 NVG患者房水及血清中VEGF、IL-6的水平明显高于对照组,且房水中二者水平呈明显正相关,提示在NVG病理机制过程中,VEGF、IL-6作为促血管生成因子,相互促进、相互影响,共同导致了NVG的发生和发展。     

新生血管性青光眼闭角期患者房水内细胞因子的变化

目的评估新生血管性青光眼(NVG)闭角期患者房水内细胞因子浓度的变化并分析其与复发的关系。方法前瞻性病例对照研究。收集2018年9月至2019年9月就诊于北京大学第三医院眼科的32例(32只眼)NVG闭角期患者(NVG组), 所有患者均接受包含抗血管内皮生长因子(VEGF)药物注射、小梁切除术和全视网膜光凝在内的三联序贯治疗并随访至少12个月。NVG组患者在抗VEGF药物注射前、小梁切除术前及NVG复发时收集房水标本, 采用流式点阵免疫发光法检测VEGF、白细胞介素(IL)、趋化因子等45种细胞因子的浓度。选取25例增生型糖尿病视网膜病变(PDR)患者(PDR组)和24例年龄相关性白内障患者(白内障组)作为疾病对照组。细胞因子浓度以M(Q1, Q3)表示, NVG组与疾病对照组间细胞因子浓度比较采用非参数Kruskal-WallisH检验;NVG复发与未复发患者间细胞因子浓度比较采用Mann-WhitneyU检验。结果 NVG组年龄(60±11)岁, 男性22例, 女性10例;年龄和性别分布与两个疾病对照组相比差异均无统计学意义(均P>0.05)。NVG组在抗VEGF治疗前、小梁...     

前部视网膜冷凝术对新生血管性青光眼患者房水中血管内皮生长因子含量的影响

目的 探讨前部视网膜冷凝术对新生血管性青光眼患者房水中血管内皮生长因子（VEGF）含量的影响以及VEGF含量的变化与虹膜新生血管之间的关系。方法对28例确诊为新生血管性青光眼患者行虹膜血管造影,确定新生血管的范围和数量后,行前部视网膜冷凝术,7～14d后经虹膜血管造影确定虹膜新生血管大部分消退后,再行小梁切除术。分别于前部视网膜冷凝术前和小梁切除术前抽取房水标本,另取30例老年性白内障患者房水标本。采用酶联免疫吸附法分别测定全部房水标本中的VEGF含量。结果小梁切除术前房水中VEGF的含量[（2．096±0．512）ng／ml]明显低于前视网膜冷凝术前房水中VEGF含量[（0．478±0．312）ng／ml],两者比较差异有统计学意义（P〈0．01）。小梁切除术前房水中VEGF含量明显高于老年性白内障患者房水中VEGF的含量[（0．198±0．045）ng／ml],两者比较差异有统计学意义（P〈0．01）。结论VEGF在虹膜新生血管的形成中可能发挥一定的作用;阻断促使虹膜产生新生血管的VEGF来源,可抑制新生血管性青光眼的发生.（中华眼科杂志．2007．43：622-625）