结肠癌术后化疗对外周血免疫细胞比例及凋亡的影响

目的研究结肠癌术后化疗患者外周血免疫细胞亚群变化特点、淋巴细胞凋亡状况及其意义。方法应用流式细胞术对40例结肠癌患者手术前后(术前1d、术后3d、化疗前1d)、化疗过程(化疗第3d、化疗后3d)中外周血T淋巴细胞亚群及NK细胞水平进行检测,以AnnexinV/PI双标流式细胞术对淋巴细胞凋亡、坏死状况进行检测。结果结肠癌根治术后3d,患者外周血T淋巴细胞及NK细胞数量较术前有所减少,但无显著差异(P>0.05)。至化疗前1d,患者外周血CD3 ,CD4 T细胞及NK细胞数量较术前显著增高(P<0.01),但CD8 T细胞数量减少(P<0.05)。化疗3d后,患者外周血CD3 ,CD4 ,CD8 T细胞及NK细胞水平较术前全面下降(P<0.05),但CD4 /CD8 比例变化不大,而淋巴细胞凋亡坏死比例明显升高。至化疗结束后3天,T淋巴细胞各亚群及NK细胞数量开始逐渐回升。结论结肠癌根治术手术本身对患者外周血淋巴细胞及NK细胞影响不大,术后患者免疫细胞水平明显升高,机体免疫状况明显改善。化疗可造成患者机体的一过性免疫抑制,可能与其造成机体淋巴细胞的凋亡坏死有关。     

大肠癌患者术后NDV-ATV治疗对细胞免疫功能的影响

目的　探讨大肠癌患者术后NDV -ATV治疗对细胞免疫功能的影响。方法　采用流式细胞仪分析 70例术后接受NDV -ATV治疗的大肠癌患者外周静脉血T淋巴细胞亚群及NK细胞活性的变化情况 ,并与 45例术后未接受NDV -ATV治疗的大肠癌患者作为对照。结果　NDV -ATV治疗组 ,术后外周血CD+3、CD+4、NK细胞和CD+4 CD+8均较治疗前显著增高 (P <0 0 1 ) ,且明显高于未接受NDV -ATV治疗的术后常规治疗组 (P <0 0 5 )。结论　NDV -ATV可改善大肠癌患者术后机体的免疫功能     

初治骨关节结核患者外周血T细胞亚群特点及临床意义

目的 探讨初治骨关节结核患者外周血T细胞亚群特点、与临床观测指标的关系及抗结核治疗对患者细胞免疫状况的影响。方法 采用单平台流式细胞术检测骨关节结核患者治疗前和治疗后1-2个月T细胞亚群的绝对数及百分比，并与恶性骨肿瘤患者和健康人比较。结果 初治骨关节结核组CD4及CD8T细胞绝对数较健康对照组低（P〈0．01）。骨关节结核患者在治疗1-2个月后CD4T细胞百分比升高（P〈0．05）。青年组CD8T细胞绝对数和百分比显著高于中年组和老年组（P〈0．05，P〈0．01），CD4／CD8显著低于中年组及老年组（P〈0.01）。T细胞亚群各参数与PPD反应直径、红细胞沉降率和C反应蛋白之间无显著相关性（P〉0．05）。脊柱结核合并脓肿组CD4／CD8较低，CD8T细胞百分比较高（P〈0．01）。结论 初治骨关节结核患者存在细胞免疫缺陷，以中老年组更明显。脊柱结核是否形成脓肿与CD8T细胞有关。初治骨关节结核患者经1～2个月化疗后，细胞免疫状况改善，是病灶清除术的有利时机．     

Impact of splenectomy on circulating T-lymphocyte subsets in stage III gastric cancer

Background: The role of splenectomy remains unclear in patients with gastric cancer who undergo total gastrectomy. The aim of this study was to prospectively evaluate the impact of splenectomy on circulating T‐lymphocyte subsets and survival in advanced gastric cancer. Methods: Analysis of lymphocyte subsets was performed in 40 patients with American Joint Committee on Cancer (AJCC) stage III gastric adenocarcinoma located on the upper one‐third of the stomach, who underwent a curative total gastrectomy with or without splenectomy. Circulating T‐lymphocyte subsets were measured on venous blood by using flow cytometry and monoclonal antibodies at preoperative day 1, and postoperative months 1, 3, 6, 12 and 18. Results: The proportion of lymphocytes and the values of CD3, CD8, CD16 and CD25 subsets were higher in the splenectomy group of patients at postoperative month 3. In the spleen preservation group at the same point of treatment, the proportion of granulocytes and the values of CD4 and CD4 : CD8 ratio were higher. Except for CD16 levels, all T‐lymphocyte subsets showed no sig­nificant difference between splenectomy and spleen preservation groups after postoperative month 3. Increased CD16 levels in the splenectomy group were not associated with improvement in patients’ 5‐year survival rates. Conclusion: These results suggest that the long‐term impact of splenectomy does not play an important role in postoperative quantitative changes of circulating T‐lymphocyte subsets of patients with stage III gastric cancer who have undergone total gastrectomy. Furthermore, splenectomy does not give a prognostic benefit, based on tumour recurrence and survival of patients with proximal one‐third gastric cancer who undergo total gastrectomy.     

儿童肾病综合征T细胞亚群检测的临床意义

目的：探讨儿童原发性肾病综合征（NS）T细胞亚群检测的临床意义。方法：对25例NS活动期和缓解期患儿应用流式细胞仪检测T2细胞亚群的变化。结果：NS活动期组CD3^ ,CD4^ ,CD4^ /CD8^ 比值，NK[CD(16 56)]^ 细胞均明显低于缓解期组（P＜0．01）和对照组（P＜0．01）。结论：说明NS细胞免疫功能减低，T细胞亚群检测可作为肾病综合征活动指标之一。     

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目的 研究乳腺癌腋窝淋巴结发生癌转移和未发生癌转移时的免疫功能。方法2004年8月至2005年7月采用流式细胞技术检测乳腺癌病人乳腺癌前哨淋巴结（SLN）与乳腺癌非前哨淋巴结（NSLN）中免疫细胞CD3^＋T、CD4^＋T、CD8^＋T、CD16^＋NK、CD^19^＋B的比例，并相互比较。结果淋巴结未发生癌转移时，SLN与NSLN的免疫细胞CD3^＋T、CD4^＋T、CD8^＋T、CD16^＋NK、CD^19^＋B的比例差异无统计学意义（P〉0．05）；而且当SLN与NSLN发生癌转移后，它们的免疫细胞的比例也无差异。但SLN发生癌转移与未发生癌转移时相比，其CD4^＋T、CD8^＋T、CD16^＋NK的比例发生显著改变（P〈0．05），CD3^＋T、CD19^＋B的比例改变无统计学意义（P〉0．05）。结论 当腋窝淋巴结未发生癌转移时，它仍有正常的免疫功能。当腋窝淋巴结发生癌转移后，它的免疫微环境发生了改变，免疫功能呈现一种抑制状态。     

晚期肺癌患者化疗前后细胞免疫功能及T细胞分泌细胞因子的变化

目的:探讨晚期肺癌患者化疗前后外周血中T细胞免疫功能及其分泌细胞因子水平的变化.方法:通过流式细胞仪检测57例晚期肺癌患者和22例健康体检者CD4+T淋巴细胞和CD8+T淋巴细胞在外周血单核细胞(PBMC)中所占比例以及外周血CD4+T细胞分泌细胞因子水平的变化.结果:晚期肺癌患者化疗前CD4+T细胞在PBMC中所占比例、CD4+T细胞与CD8+T细胞比值均低于健康人(P＜0.001),化疗后均较化疗前明显升高(P＜0.01或P＜0.001):化疗前CD8+T细胞在PBMC中所占比例高于健康人(P＜0.001),而于化疗后较化疗前明显下降(P＜0.05).治疗前后患者血清中CD4+T细胞分泌Th1细胞因子(IFN-y、TNF-α、IL-2)和Th2细胞因子(IL-4、IL-6、IL-10)水平均无明显变化(P＞0.05).结论:化疗能够增加肺癌患者外周血中CD4+T细胞所占比例,但对Th1、Th2所分泌的相关细胞因子水平影响不大.     

Correlation between radiculalgia and counts of T lymphocyte subsets in the peripheral blood of patients with lumbar disc herniation

Objective: To determine the correlation between the degree of radiculalgia and counts of T lymphocyte subsets in the peripheral blood of patients with lumbar disc herniation. Methods: Forty‐nine patients with lumbar disc herniation (group A) were divided into three subgroups according to Visual Analogue Scale (VAS) pain scores (group A₁: n= 12, VAS 0–4.0; A₂: n= 24, VAS 4.1–7.0; A₃: n= 13, VAS 7.1–10.0. Twenty health blood donors who volunteered to be involved in the study comprised the control group (group B). Peripheral blood counts of various T lymphocyte subsets were measured in each group. Results: (i) The counts of CD4+ T and CD4+/CD8+ lymphocytes were higher in group A than in group B, and the difference between the two groups was statistically significant (P < 0.05). There were also statistically significant differences between group A and group B in the counts of CD3+ and CD8+ T lymphocytes (P < 0.05); (ii) There was no correlation between the VAS scores and the counts of CD3+ T lymphocytes (r= 0.194, P > 0.05). A strong significant correlation was observed between the VAS scores and counts of CD4+ T lymphocytes (r= 0.542, P < 0.05), CD4+/CD8+ (r= 0.468, P < 0.05), which increased with increasing VAS scores in the three subgroups of group A (P < 0.05). However there was a significant negative linear correlation between CD8+ T lymphocyte counts and pain scores (r=?0.462, P < 0.05). Conclusion: Our results suggest that changes in T lymphocyte subsets in peripheral blood take place after prolapse of lumbar intervertebral discs. The current results may provide support for involvement of immunologic mechanisms in low back pain secondary to herniation of the lumbar disc. T lymphocytes may play an important role in the development of symptoms in patients with lumbar intervertebral disc herniation.     

Immunocytochemical Analysis of Cellular Infiltrates in Human Appendicitis

This study was conducted to determine the immunologic cellular composition in human ap-pendicitis and its association with the development of perforated appendicitis. Appendiceal specimens from 27 patients with acute appendicitis were immunostained to detect lymphocyte surface markers. Moreover, the lymphocyte surface markers of peripheral blood were analyzed by laser flow cytometry in 12 patients. Helper T lymphocytes (CD4) were present in all the patients, while B lymphocytes (CD19), natural killer (NK) cells (CD56), and cytotoxic T lymphocytes (CD8) were present in 7 (70%), 10 (100%), and 9 patients (90%) with perforated appendicitis, and in 12 (63.2%), 10 (58.8%), and 6 (54.5%) patients without perforation, respectively. There were significant differences between the patients with a perforated appendix and those without perforation, in the positivity rate for CD8 and CD56 cells (P < 0.05). The number of cells positive for CD56, being NK cells, in the blood from the patients with perforation was significantly lower than that in the blood from those without perforation (P < 0.05). The infiltration of a greater number of cytotoxic T lymphocytes and NK cells was observed in the appendices from patients with perforated appendicitis than in those from patients with nonperforated appendicitis. Received: December 8, 1999 / Accepted: July 25, 2000     

Variation of bronchoalveolar lymphocyte phenotypes with age in the physiologically normal human lung

BACKGROUND: Changes in T lymphocyte subsets have been observed in various forms of pulmonary disease. However, bronchoalveolar lymphocyte subsets have not been well characterised for healthy individuals differing in age. A study was undertaken to investigate the bronchoalveolar lavage (BAL) and peripheral blood lymphocyte subsets in clinically normal volunteers of two different age groups (19-36 and 64-83 years). METHODS: Bronchoalveolar lavage was performed on all individuals in both age groups and peripheral venous blood was drawn just prior to BAL. Bronchoalveolar cell profiles were characterised by morphological criteria, and cell surface antigen expression of lymphocytes was determined by flow cytometry. RESULTS: A significant increase in total BAL lymphocytes was observed for the oldest group compared with the youngest age group. Mean lymphocyte subset (CD4+/CD8+) ratios were significantly increased in BAL fluid from the older group compared with the younger group (mean (SE) 7.6 (1.5) vs 1.9 (0.2); p<0.0001). The increase in the BAL CD4+/CD8+ T cell ratio was mostly due to an increase in relative numbers of CD4+ lymphocytes, and the BAL CD4/CD8 ratio was disproportionately increased compared with peripheral blood in the older group. Increased expression of HLA-DR and CD69 on CD4+ T lymphocytes was observed in the oldest age group. Relative numbers of natural killer (NK) cells did not vary with age, and gammadelta T cells and CD5+ B cells were present in very low numbers in both age groups. CONCLUSIONS: CD4+ T cells accumulate in air spaces of the lower respiratory tract with age in healthy adults and express increased amounts of HLA-DR and CD69 on their surfaces, suggesting a relative degree of CD4+ T lymphocyte activation for healthy older individuals who have normal lung function.     

The role of lymphocyte surface binding sites for wheat germ agglutinin in the negative regulation of cancer patients

The role of lymphocyte surface binding sites for wheat germ agglutinin (WGA) in the negative regulation of cancer patients was investigated. The number of WGA binding sites on the surface of each lymphocyte ranged from 10⁷ to 10⁸. Fluorescein isothiocyanate (FITC)-conjugated WGA, bound to the majority of peripheral blood lymphocytes (PBL) with two peaks of fluorescent intensity was expressed either dimly or brightly. The increase in lymphocytes brightly expressing WGA fluorescent intensity (WGA bright lymphocytes) significantly correlated with the number of WGA binding sites. The suppression of lymphocyte proliferation mediated by the purified soluble suppressor factor (SSF) significantly correlated with an increase in the WGA bright lymphocyte population (P<0.05). A significantly greater number of WGA bright lymphocytes in PBL was found in patients with esophageal, gastric, breast, or colon cancer, than in those with benign diseases or in healthy controls. Furthermore, an increase in WGA bright lymphocytes was found in subsets expressing the antigens CD8 dimly or CD16. Thus, it is suggested that the number of WGA binding sites may increase mainly on the surface of effector cells such as NK cells and CD8-positive killer T cells in cancer patients, triggering the negative regulation mediated by SSF.     

2型糖尿病肾病患者外周血CD28/CTLA4共刺激分子的表达及其意义

目的:探讨2型糖尿病(T2DM)肾病患者外周血T细胞亚群、共刺激分子CD 28及细胞毒T淋巴细胞抗原(CTLA)4的表达及其临床意义。方法:选取2015年07月~2018年06月至我科室和内分泌科治疗的患者,根据尿微量清蛋白排泄率(UAER)将其分为DM组(T2DM无肾病,n=42)和DN组(T2DM合并肾病,n=40),同时选取健康人群40例作为对照(NC组)。采用流式细胞技术测定两组患者外周血T淋巴细胞上CD4、CD8的表达,并用流式细胞技术测定CD4^+、CD8^+T淋巴细胞表面CD28、CTLA4分子的表达。结果:三组外周血CD4^+和CD4^+/CD8^+按NC组-DM组-DN组顺序均呈显著递增趋势(P<0.01),CD3^+、CD8^+按照顺序呈显著递减趋势(P<0.01),且各组间差异明显(P<0.01);三组外周血CD4^+CD28^+、CD8^+CD28^+ T细胞按NC组-DM组-DN组顺序均呈显著递增趋势(P<0.01),CD4^+CTLA4+及CD8^+CTLA4+T细胞按照顺序呈显著递减趋势(P<0.01),且各组间差异明显(P<0.01)。结论:T2DM患者T细胞亚群失衡,其中合并肾病患者表达失衡更为严重,提示糖尿病肾病患者存在自身免疫调节异常。同时T2DM合并肾病患者外周血CD28和CTLA4表达也显著异于正常对照,提示共刺激分子可能参与了T2DM合并肾病的免疫功能紊乱。     

Lymphocyte subsets in renal allograft recipients with chronic hepatitis C virus infection.

BACKGROUND: The pathogenetic mechanisms of chronic hepatitis C virus (HCV) infection in renal allograft recipients are not well established. This study aimed to examine the relationship between altered immune status and HCV-related liver disease, by determining the changes in peripheral blood lymphocyte and natural killer (NK) cell subsets in these subjects. METHODS: Peripheral blood lymphocyte, NK cell and activation markers were detected by flow cytometry in renal allograft recipients with (TpC+) or without (TpC-) HCV infection, and compared with age- and sex-matched patients with post-transfusional chronic HCV infection (TfC+) and healthy controls. RESULTS: CD19+ cells were reduced in renal allograft recipients compared with controls. TpC+ subjects had increased CD3+CD8+ cells compared with controls, and increased CD3+DR+ cells but reduced CD4+ CD38+ and CD3-CD16/56+ cells compared with controls as well as TfC+ patients. TfC+ patients and controls had similar numbers and proportions for the lymphocyte subsets and NK cells. Chronic liver disease in HCV-infected renal allograft recipients was associated with increased CD3+CD16/56+ cells but reduced CD4+CD38+ cells. Reduction of CD3-CD16/56+ cells was noted in TpC+ subjects without liver disease. Yet among post-transfusional (TfC+) subjects this was associated with chronic hepatitis. CONCLUSIONS: Peripheral blood suppressor/cytotoxic T lymphocytes are increased, whereas activated helper/inducer T lymphocytes and NK cells are reduced, in renal allograft recipients with HCV infection. Increased non-MHC-restricted cytotoxic T cells and reduced NK cells are associated with the presence or absence of liver disease respectively. These data suggest that immune mechanisms are important in the pathogenesis of chronic hepatitis C after renal transplantation.     

膀胱肿瘤患者红细胞免疫功能的变化

目的 探讨膀胱肿瘤患者的红细胞免疫功能状态及与Ｔ淋巴细胞亚群改变的关系。方法 测定３４例膀胱移行细胞癌患者的红细胞Ⅰ型补体受体花环率（Ｃ３ｂＲＲ）、免疫复合物花环率（ＩＣＲ）及４项肿瘤红细胞花环率［直向肿瘤红细胞花环试验（ＤＴＥＲ）、促肿瘤红细胞花环试验（ＥＴＥＲ）、协同肿瘤红细胞花环试验（ＡＴＥＲ）及自然肿瘤红细胞花环试验（ＮＴＥＲ）］,同时用流式细胞仪（ＥＴＥＲ）检测Ｔ细胞亚群,与年龄相近的３     

淋巴细胞亚群对乳腺癌新辅助化疗疗效的预测价值

目的 本研究旨在探讨治疗前淋巴细胞亚群对乳腺癌新辅助化疗(NAC)疗效的预测价值.方法 本研究选取2016年4月至2020年6月在中山大学孙逸仙纪念医院接受NAC的乳腺癌患者109例.采用卡方检验及logistics回归分析不同淋巴细胞亚群比例与病理完全缓解(pCR)的相关性,并通过Kaplan-Meier曲线评估其与...     

Influence of multimodality therapy on the cellular immunity of patients with esophageal cancer

Background: Cancer patients have often been reported to have impaired immune function, and the effect of treatment modalities, such as surgery, irradiation, and chemotherapy, in depressing patients’ immunity has also been reported. In this investigation, the effect of treatment on the cellular immunity of esophageal cancer patients was evaluated. Methods: Immunological parameters, such as natural killer (NK) activity and lymphocyte subsets in peripheral blood, were measured in 32 esophageal cancer patients on 5 occasions (on the day of admission, 2 days before surgery, and 1 week, 1 month, and 2 months after surgery). Results: NK activity was greatly impaired shortly after the operation, and the percentages of lymphocytes as a whole, and CD8⁺, CD16⁺, and CD57⁺ lymphocytes were significantly decreased, on the other hand, a postoperative increase in the CD4⁺/CD8⁺ ratio was observed. No significant depression of immune function by postoperative irradiation was observed. Conclusions: The results of this study suggest that cellular immunity, especially cytotoxicity, shortly after esophagectomy may be greatly impaired by the surgical stress of esophagectomy and an added effect of chemotherapy.     

蜕膜自然杀伤细胞与不明原因反复自然流产的关系

目的通过测定子宫蜕膜自然杀伤(NK)细胞亚群及其杀伤活性,探讨其在不明原因反复自然流产(URSA)发病中的作用。方法随机选取确定URSA的患者10例为实验组,选取15例正常妊娠(NP组)为对照,采用流式细胞仪检测两组蜕膜组织NK细胞亚群,MTT还原法测定NK细胞杀伤活性。结果URSA组蜕膜中CD56+NK细胞含量显著高于NP组(P<0.01),且URSA组CD56+CD16+细胞亚群含量及CD56+CD16+/CD56+CD16-比值也较NP组显著升高(P均<0.01),而CD56+CD16-细胞亚群含量两组比较无差异(P>0.05)。蜕膜NK细胞活性URSA组为(31.60±2.62)%,而NP组仅为(15.65±2.02)%,两组蜕膜NK细胞活性有显著差异(P<0.01)。结论URSA的发生可能与NK细胞亚群失衡及杀伤活性异常增高而导致母胎间的免疫耐受平衡被打破有关。     

CD3AK细胞过继治疗对肝癌患者T淋巴细胞亚群及功能活性的影响

目的 探讨CD3AK细胞过继治疗对原发性肝癌（PLC）患者T淋巴细胞亚群及功能活性的影响。方法 58例 PLC患者分为:CD3AK细胞治疗组（n=37）,对照组（n=21）,另取11例献血员为正常对照。治疗组术后第1天开始输注CD3AK细胞,每天1次,每次输注2x109个细胞,输注前30min静脉注射IL-2100u,连续5d为一个疗程;对照组只给予与治疗组相同的保肝治疗。观察两组T淋巴细胞亚群、IL-2R、细胞增殖功能、NK及LAK活性。结果 治疗组CD4/CD8比值、NK活性、LAK活性、IL-2R表达水平及细胞增殖活力与治疗前和对照组比较,差异均有显著性意义。结论 过继输注CD3AK细胞治疗能够补充肝癌患者有效抗肿瘤活性细胞数量的不足,并能够使T淋巴细胞亚群比例失调和功能低下的状况获得不同程度的改善。     

Immuno-modulatory effects of relaxation training and guided imagery in women with locally advanced breast cancer undergoing multimodality therapy: A randomised controlled trial

Eighty women undergoing multimodality treatment for large (>4 cm) or locally advanced (T3, T4, Tx, N2), breast cancers participated in a randomised controlled trial (RCT) to evaluate the immuno-modulatory effects of relaxation training and guided imagery. Patients underwent chemotherapy followed by surgery, radiotherapy, and hormone therapy. Those in the intervention group were taught relaxation and guided imagery. Patients kept diaries of the frequency of relaxation practice and imagery vividness. On 10 occasions during the 37 weeks following the diagnosis, blood was taken for immunological assays CD phenotyping: T cell subsets (helper, cytotoxic), natural killer (NK) and lymphokine activated killer (LAK) cells, B lymphocytes and monocytes; cytotoxicity: NK and LAK cell activities; cytokines interleukin 1 beta (1β), 2, 4 and 6 and tumour necrosis factor alpha.Significant between-group differences were found in the number of CD25+ (activated T cells) and CD56+ (LAK cell) subsets. The number of CD3+ (mature) T cells was significantly higher following chemotherapy and radiotherapy, in patients randomised to relaxation and guided imagery. Using a median split, women who rated their imagery ratings highly had elevated levels of NK cell activity at the end of chemotherapy and at follow-up. Significant correlations were obtained between imagery ratings and baseline corrected values for NK and LAK cell activity, and IL1β. Relaxation frequency correlated with the number of CD4+ (T helper) cells, the CD4+:8+ (helper:cytotoxic) ratio, and IL1β levels.Relaxation training and guided imagery beneficially altered putative anti-cancer host defences during and after multimodality therapy. Such changes, to the best of our knowledge, have not been previously documented in a RCT.     

槐耳对胰腺癌切除术后化疗病人免疫功能的影响

目的 探讨胰腺癌切除术后吉西他滨(GEM)化疗结合槐耳综合治疗对病人免疫功能的影响.方法 选择我院胰腺外科中心2004年7月～2006年9月胰腺癌切除术后病人66例,其中,A组33例(GEM化疗),B组33例(GEM化疗 槐耳治疗);另取同期未行以上治疗的17例作为空白对照,为C组.分析比较各组的体液及细胞免疫功能指标.结果 IgA、IgG、IgM值方面,A组治疗前后的变化差异无统计学意义(P＞0.05),B组治疗结束后的IgG、IgM值显著高于治疗前(P＜0.05);治疗结束后,B、C组IgA、IgG、IgM值高于A组(P＜0.05).淋巴细胞亚群值方面,A、B组治疗前后的组内变化差异均无统计学意义(P＞0.05);治疗结束后,A组各淋巴细胞亚群值均低于C组(P＜0.05),而NK细胞、CD3 T细胞亚群百分比及CD4 /CD8 比值低于B组(P＜0.05).结论 槐耳辅助治疗可提高胰腺癌术后GEM化疗病人的体液及细胞免疫功能指标.