Observations on the effects of form deprivation on the refractive status of the monkey

The consistency of the refractive error alterations produced by monocular form deprivation in developing monkeys and the influence of the duration and the age at the onset of deprivation on the magnitude of these alterations was investigated. Refractive error and axial length measurements are presented for a group of monkeys which had one eye sutured closed for a period exceeding 18 months beginning at various ages ranging from 26 days to 25 months. In addition, we pooled and reanalyzed refractive error and axial length data for monocularly form-deprived monkeys from previous studies. When the alterations in the deprived eye's refractive status are specified with respect to the fellow nondeprived eye, the results are, with a few noteworthy exceptions, consistent between laboratories and individual animals. In most cases, early monocular form deprivation causes the treated eye to develop a longer axial length and to manifest a more myopic/less hyperopic refractive error than the fellow nontreated eye. The magnitude of this deprivation-induced alteration is generally dependent on the duration and the age at the onset of form deprivation. The earlier the deprivation is initiated and the longer it is maintained, the greater the degree of the relative myopia produced in the deprived eye.     

Histologic studies on the effect of light coagulation of the retina on the vitreous body

Up to 6 months after panretinal photocoagulation of rabbit eyes the subsequent changes in the vitreous body were examined histologically by means of a stereomicroscope. Marked changes were evident: severe exudation into all of the vitreous tissue during the early follow-up period, circumscribed areas of higher density or loss of normal density, membrane-like structures, confluent cavities and liquefaction of most of the tissue at the later postcoagulative stage.     

屈光参差对对比敏感度的影响

目的探讨屈光参差的程度对对比敏感度的影响。方法随机选择双眼屈光参差但矫正视力≥1．0的患者38例（76眼），平均年龄（29．61±8．78）岁（19～53岁），等效球镜值为（-6.57±3．64）D（0．25- -14．50D），双眼度数相差（-3．67±2．40）D（-1．50- -12．00D）。按屈光参差的程度将患者分成两组：A组44眼。双眼度数相差≥3．00D；B组32眼，双眼度数相差〈3．00D。使用Stereo Optical公司生产的OPTEC6500型眩光对比敏感度测试系统分别测试患者在最佳矫正视力下左右眼的对比敏感度。应用SPSS11．5统计软件对结果进行配对比较的秩和检验。结果A组患者屈光度高的一眼较另一眼在中高频空间频率的对比敏感度下降，在空间频率为6．0c／d、12．0c／d和18．0c／d时，低屈光度眼与高屈光度眼的对比敏感度差值分别为6．54、12．04和6．27．P值分别为0．017、0．001和0．006．差异有显著性；而在低空间频率（1．5c／d和3．0c／d），双眼的对比敏感度差值分别为3．54和5．41，P值分别为0．266和0．254，双眼间的对比敏感度差异无显著性。B组患者左右眼的对比敏感度在各个空间频率（1．5c／d、3．0c／d、6．0c／d、12．0c／d和18．0c／d）中低屈光度眼与高屈光度眼的对比敏感度差值分别为-1．24、5．75、3．00、2.32和3．00，差异均无显著性．P值分别为0．824、0．327、0．789、0．419和0．062。结论3．00D以上的屈光参差影响中高频空间频率的对比敏感度，3．00D以下的屈光参差不对对比敏感度产生明显影响。     

纹眼线对于干眼症发病的影响

目的:研究纹眼线对于干眼症发病的影响。方法:选择127例患有干眼症的患者,其中46例纹过眼线,其余81例未纹过眼线。比较两组患者的初次发病年龄、泪膜破裂时间、Shirmer试验、结膜印迹细胞,以及纹眼线患者纹眼线与干眼症初次发病之间的间隔时间。结果:纹眼线患者纹眼线与发病的间隔时间平均为13.4a,且纹眼线患者干眼发病的年龄有所提前。两组患者其他的检查结果无统计学差异。结论:纹眼线会使干眼症的发病年龄提前,但却不会影响初期发病的严重程度。纹眼线对于干眼症的病程及治疗的影响仍需进一步探讨。     

苦参碱抑制视网膜母细胞瘤细胞增殖的研究

目的 探讨中药成分苦参碱（Ma）对视网膜母细胞瘤细胞株（HXO-Rb44细胞株）增殖的抑制作用及相关作用机制，为中药综合治疗视网膜母细胞瘤提供实验依据。设计 实验性研究。研究对象 体外培养的HXO-Rb44细胞。方法 0．4mmol／L的Ma分别作用HXO-Rb44细胞12、24、48小时，流式细胞仪检测S期细胞百分比的变化；通过细胞免疫组织化学染色结合计算机自动图像分析技术，测量阳性细胞积分光密度（IOD）值，观察分析Ma对HXO-Rb44细胞内增生靶基因PCNA的调控。主要指标 S期细胞百分比（SPF）、积分光密度值（IOD）。结果 0．4mmol／L浓度Ma作用HXO-Rb44细胞后，S期细胞百分比下降（P均〈0．01），细胞内PCNA蛋白表达水平下降（P均〈0．01），并均呈时间依赖性。结论 Ma能够抑制HXO-Rb44细胞增殖。提示Ma有望成为治疗视网膜母细胞瘤的有效药物。