Clinical Observation on Shuanghuanglian powder in treating viral myocarditis of childrenin treating viral myocarditis of children

Objective: To study immune function of children viral myocarditis and to evaluate the clinical effect of Shuanghuanglian Powder (SHLP) by injection.Methods: The 62 patients of viral myocarditis were divided into two groups randomly, the SHLP group (n = 32) treated with conventional therapy plus SHLP and the conventional treatment group (n = 30) with conventional therapy alone. Their serum antibody of Coxsackie virus group B (COXB-IgM), T-lymphocyte subsets including CD₃ ⁺, CD₄ ⁺, CD₈ ⁺ and CD₄ ⁺/CD₈ ⁺ were determined with ELISA and indirect immunofluorescent assay.Results: COXB-IgM was positive in 39 of the 62 patients, which was significantly different with those of normal controls (P< 0.001). Patients’ serum level of CD₄ ⁺ cells and CD₄ ⁺/CD₈ ⁺ ratio decreased while CD₈ ⁺ increased. After treatment, the recovery of symptoms, signs and immune function in patients of the SHLP group were significantly better than those in patients treated with conventional treatment alone (P<0.01).Conclusion: Immunoregulatory disturbance is involved in children with viral myocarditis and SHLP is an effective drug in the treatment of children viral myocarditis.     

Determination of CD30 expression on peripheral blood T lymphocyte subsets in patients with hemorrhagic fever with renal syndrome by FCM

Summary To determine the CD₃₀ expression on peripheral blood T lymphocyte subsets in patients with hemorrhagic fever with renal syndrome (HFRS) and its clinical implications, double immunofluorescence technique and flow cytometry were used. There was no significant difference among the severe group, mild-moderate group and normal control group in the CD+CD₃₀ T lymphocyte subset. While the CD₄ ⁺CD₃₀ ⁻ T cells of HFRS patients were increased and the difference between severe group and mild-moderate group or normal control group were very significant (P<0.01) and the difference between the mild-moderate group and normal control group was also significant (P<0.05). The CD₈ ⁺CD₃₀ ⁻ T cells were increased while the CD₈ ⁺CD₃₀ ^- T cells decreased obviously in HFRS patients, and the differences among three groups in both subsets were very significant (P<0.01). The results showed that the humoral immunity and cellular immunity are overactive in HFRS patients during acute phase. The loss of balance between T lymphocyte subsets may play an important role in the pathophysiology of HFRS and is closely correlated with the severity of the HFRS. This project is supported by the grant of the Ministry of Health (Serial No. 96-2-116).     

IGF-1在妊娠期糖尿病胎盘组织中的表达及其对新生儿T淋巴细胞亚群的影响

目的 分析胰岛素样生长因子-1(IGF-1)在妊娠期糖尿病(GDM)胎盘组织中的表达及其对新生儿T淋巴细胞亚群的影响。方法选取本院GDM患者(研究组)和同期健康孕妇(对照组)各40例。两组孕妇均于胎儿、胎盘分娩后于胎盘中央带取材,行常规HE染色和IGF-1抗免疫组织化学染色,比较两组孕妇胎盘组织中IGF-1表达强度和分娩的新生儿T淋巴细胞亚群指标的差异,并进行相关性分析。结果研究组IGF-1表达强于对照组。IGF-1主要存在于滋养层上皮细胞胞浆和少数血管内皮细胞中。与对照组孕妇新生儿比较,研究组孕妇新生儿CD⁺₃、CD⁺₄、CD⁺₄/CD⁺₈显著较低,CD⁺₈显著较高。相关性分析结果显示孕妇胎盘组织中IGF-1表达强度与CD⁺₃、CD⁺₄、CD⁺₄/CD⁺₈呈正相关,与CD⁺₈呈负相关。结论GDM胎盘组织中的IGF-1表达水平较健康孕妇高,GDM胎盘组织中的高IGF-1表达可显著影响新生儿T淋巴细胞亚群,导致新生儿免疫功能低下。     

自体CIK细胞对晚期不同阶段肿瘤患者免疫状态和生活质量的影响

目的 探讨自体细胞因子诱导的杀伤细胞（CIK）治疗晚期恶性肿瘤患者,对其免疫状态和生活质量的影响。方法 选取2013年2月至2016年3月合肥市第一人民医院血液肿瘤科收治的42例晚期肿瘤患者,按照预计生存期分为A组（预计生存期<3个月）20例、B组（预计生存期≥3个月）22例,检测治疗前后A、B组患者外周血T细胞亚群的变化。观察治疗前后A、B组患者免疫功能的变化,生活质量改善情况及治疗相关不良反应。结果 CIK细胞治疗后,A组患者CD₃⁺T细胞水平较治疗前降低（P<0.05）;B组治疗后CD₃⁺、CD₄⁺和CD₄⁺/CD₈⁺水平较治疗前明显升高（P<0.05）,CD₈⁺T淋巴细胞亚群水平较治疗前降低（P<0.05）。A、B两组患者治疗后KPS评分分别较治疗前均有提高,其中A组差异无统计学意义（P>0.05）,而B组差异有统计学意义（P<0.05）。42例患者在输注CIK过程中未出现明显不良反应。结论 CIK细胞回输安全、副作用小,自体CIK细胞治疗可提高晚期恶性肿瘤患者的免疫功能,改善其生活质量,但应尽早进行。     

Clinical Observation on the Effect of Zengse Pill （增色丸） in Treating Patients with Vitiligo of Qi-stagnancy and Blood-stasis Syndrome Type

Objective： To observe the clinical efficacy of Zengse Pill （增色丸, ZSP） on patients with vitiligo of qi-stagnancy and blood-stasis syndrome type （V-QB）, and to preliminarily explore its mechanism of action. Methods： Sixty-five V-QB patients, with their diagnosis confirmed by clinical examination, were randomized by digital table method into two groups, with 31 patients in the control group and 34 in the treatment group. Cobamamide （2 tablets） was administered orally to all patients, and Psoralea tincture （a self-formulated preparation） was applied externally thrice a day. In addition, for patients in the treatment group, ZSP was given orally, at 5 pills per dose, 3 times every day. The therapeutic course for both groups was 3 months. Patients were re-examined every half-month, and changes in the skin lesions were observed and recorded. The levels of lymphocyte subsets, serum immune globulin, and complement C3 and C4 in patients were determined before and after the therapeutic course and compared with the corresponding indexes determined in 21 healthy subjects, Results： The total effective rate in the treatment group was 82.4%, which was markedly higher than that in the control group （54.8%）, showing a significant difference （P〈O.05）. After treatment, CD4^＋ percentage, CD4^＋/CD8^＋ratio, and blood levels of C3 and C4 increased, while CD8^＋ percentage decreased in the treatment group （P〈0.05 or P〈0.01）. All these indexes remained unchanged in the control group, and the respective comparisons between groups showed significant differences （P〈0.01）. Conclusion： ZSP has a definite clinical effect on the treatment of V-QB but with no evident adverse reactions, and it can increase the CD4^＋ percentage, CD4^＋/CD8^＋ ratio, and the levels of serum C3 and C4, thus regulating the immunity of the organism, which might be one of its mechanisms of action.     

Study on clinical effect and mechanism of Jianpi Qingre Huayu Recipe (健脾清热化瘀方) in treating patients with gastric ulcer

Objective： To study the effects of Jianpi Qingre Huayu Recipe （健脾清热化瘀方, JQH） in curing gastric ulcer and to preliminarily probe into its pathogenic mechanism, Methods： Fifty patients with gastric ulcer of Pi （脾）-insufficiency and stasis-heat syndrome type were assigned to the treated group （30 patients） and the control group （20 patients）. They were treated respectively with JQH and Ranitidine. At the same time, another group consisting of 20 healthy persons was set up for normal control. The clinical effect on gastroscopic figure and traditional Chinese medicine （TCM） syndrome were observed. Changes of T-cell subsets and interleukin-8 （IL-8） in serum as well as IL-8 in mucosa around the gastric ulcer were determined before and after treatment by flow cytometry and ELISA. Results： Comparison of the total effective rate on gastroscopic figure in the treated group and the control group （86.7% vs 80.0%） showed insignificant difference, but the cure rate and markedly effective rate in the former （50.0% and 20.0%） was higher than that in the latter （40.0% and 15.0%） respectively. Comparison of the total effective rate on TCM syndrome in the treated group and in the control group （96.7% vs 70.0%） showed insignificant difference, but the cure rate and markedly effective rate in the former （63.3% and 23.3%） was higher than that in the latter （50.0% and 20.0%） respectively. Serum levels of CD3^＋, CD4^＋, CD8^＋ got restored to normal range in the treated group after treatment but it was not so in the control group. IL-8 level in gastric mucosa was improved in both groups but the improvement in the treated group was better. Cenclusien： JQH could effectively treat gastric ulcer and partly reduce its recurrence through improving patients＇ immune function.     

他克莫司联合中药热敷及激光照射改善白癜风患者的疗效及机制

观察在他克莫司软膏治疗的基础上配合中药热敷与308nm准分子激光照射对白癜风的治疗效果的影响,并探讨其机制。结果显示中药热敷与308nm准分子激光照射能改善他克莫司对白癜风的治疗效果,并且可提高患者血清CD₄⁺,CD₂₅⁺,Treg,CD₄⁺CD₂₅⁺FOXP₃⁺Treg浓度。表明他克莫司联合中药热敷、308nm准分子激光照射治疗白癜风可显著提高临床疗效,其机制可能通过调控调节性T细胞CD₄⁺CD₂₅⁺FOXP₃⁺改善机体免疫机能。     

Effect of Tiotropium Bromide on Expression of CD_8~＋CD_（25）~＋FoxP_3~＋ Regulatory T Cells in Patients with Stable Chronic Obstructive Pulmonary Disease

The expression of CD8+CD25+FoxP3+ regulatory T cells(CD8+Tregs) in the peripheral blood of patients with stable chronic obstructive pulmonary disease(COPD),and the effect of muscarinic cholinergic receptor antagonist tiotropium bromide on the expression of CD8+Tregs were investigated.Twenty-three patients with moderate to severe stable COPD were enrolled in this study.All patients inhaled tiotropium bromide(18 μg daily) for 3 months.Before and after inhalation of tiotropium bromide,peripheral blood samples were collected from the patients,and T cells were labeled by three-color labeled monoclonal antibodies.Flow cytometry was used to detect the quantity and percentage of CD8+T cells,CD8+CD25+T cells,CD8+Tregs,CD4+T cells,CD4+CD25+T cells and CD4+CD25+FoxP3+ regulatory T cells(CD4+Tregs) respectively.The percentage of CD4+T cells was increased from(27.82±2.18)% to(35.53±1.3)%(t=3.20,P=0.004) in the peripheral blood of patients with stable COPD after inhalation of tiotropium bromide for 3 months,that of CD4+CD25+T cells was decreased from(10.03 ±1.42)% to(4.21 ±0.65)%(t=3.78,P=0.001),and that of CD8+Tregs was increased from(8.41 ±1.68)% to(21.34 ±4.20)%(t=2.72,P=0.013).At baseline,CD8+T cells,CD8+CD25+T cells and CD4+Tregs were detectable in the peripheral blood,but no significant changes were observed after treatment.Linear correlation analysis revealed that the difference before and after treatment in CD4+T cells and CD4+CD25+T cells was negatively correlated with the ratio of change in CD8+Tregs before and after treatment(r=-0.61,P=0.013;r=-0.72,P=0.001 respectively).In the peripheral blood of patients with stable COPD,there was the expression of CD8+Tregs and CD4+Tregs.Muscarinic receptor antagonist,tiotropium bromide,can promote the amplification of CD4+T cells,inhibit the expression of CD25+T cells,and enhance the expression of CD8+Tregs.CD8+Tregs and CD4+Tregs can be used as new indicators to understand the immune status of patients.They are helpful in judging the treatment efficacy and disease immunophenotype.     

孟鲁司特治疗重度慢性阻塞性肺疾病急性加重对免疫功能影响的分析

目的探讨孟鲁司特联合舒利迭治疗重度慢性阻塞性肺疾病（COPD）急性加重对免疫功能的影响。方法将113例重度COPD急性加重期患者,随机分为孟鲁司特联合舒利迭治疗组57例,单纯舒利迭治疗组56例,治疗14 d后,评价所有患者治疗前、后T细胞亚群CD₃⁺、CD₄⁺及CD₄⁺/CD₈⁺的变化。结果治疗组CD₃⁺、CD₄⁺、CD₄⁺/CD₈⁺指标差异有统计学意义（P<0.05）,而对照组无明显变化,药物冶疗安全性好。结论孟鲁司特钠联合舒利迭治疗中重度COPD急性加重期,能有效改善患者免疫功能,提高患者免疫力。     

Study on abnormality and regulation of T-Lymphocyte subsets in peripheral blood of rheumatoid arthritis patients

Objective:ToinvestigatethechangeofT lymphocytesubsetsinperipheralbloodofrheumatoidarthritis (RA) patientsandanalyzetheeffectsofFuzhengQubiDecoction (FZQBD)onT cellsubsets.Methods:ThirtyRApatientswererandomlydividedintotwo groups,andtreatedwithFZQBDorweste…     

Bailong tablet and chemotherapy in the treatment of moderate and advanced cancersand chemotherapy in the treatment of moderate and advanced cancers

Objective: To determine the effectiveness of Bailong Tablet and chemotherapy on treatment of moderate and advanced cancers.Methods: The patients were divided randomly into two groups. The treated group (n= 41) was treated with chemotherapy plus Bailong Tablet; and the control group (n= 22) treated with chemotherapy alone. Treatment effectiveness was measured with Karnofsky scores.Results: Karnofsky performance status in the treated group before and after treatment was 66.0 ± 8.7 and 68.0 ± 5.9 respectively, that in the control group was 67. 0 ± 6.8 and 53. 0 ± 7.3 respectively. The completion rate of chemotherapy in the treated group (92.6%, 38/41) was higher than that in control (77.2%, 17/22,P < 0.05). The side-effects caused by chemotherapy in the treated group were lower than that in the control group (P < 0.05). CD₃ ⁺, CD₄ ⁺ and CD₄ ⁺ /CD₈ ⁺ ratio were higher in the treated group.Conclusion: Bailong Tablet can enhance the therapeutic effect and reduce the side-effects of chemotherapy. One possible mechanism is the modulation of cellular immune function.     

Effect of TCM combined with chemotherapy on immune function and quality of life of patients with non-small cell lung cancer in stage III-IV

Objective: To observe and compare the effect of traditional Chinese medicine (TCM) combined with chemotherapy (CT) on immune function and quality of life (QOL)of patients with non-small cell lung cancer (NSCLC) in stage III-IV.Methods: One hundred cases with stage III-IV NSCLC were randomly divided into two groups. The treated group (n = 50) received CT combined with TCM, and the control group received CT alone. The percentage of T lymphocyte subset in peripheral blood and the change of natural killer (NK) cell count were observed after treatment. The QOL and tolerance of CT were also compared between the two groups after treatment. Results: In the treated group, CD₃ cell count, CD₄ cell count, CD₄/ CD₈ ratio and NK cell activity were higher than those in control group, while CD₈ cell count in the treated group was lower than that in the control group (P<0.05), and QOL and tolerance of CT in the treated group were also better (P<0.05).Conclusion: TCM combined with CT could raise the patients’ ability in tolerating CT in stage III-IV NSCLC.     

乳腺癌患者细胞免疫水平与预后危险因素的关系及临床意义

目的：探讨乳腺癌患者细胞免疫状态与预后危险因素之间的关系。方法：回顾性分析1997年至2002年收治的110例女性乳腺癌患者的临床资料,分析其一般情况及CD⁺₃T、CD⁺₄T、CD⁺₈T、CD⁺₄/CD⁺₈、淋巴细胞转化率（LTR）的变化及其与乳腺癌肿块大小、淋巴结转移情况及癌胚抗原（CEA）的关系。结果：①乳腺癌不同肿块大小患者中CD⁺₄T、CD⁺₈T、CD⁺₄/CD⁺₈水平差异有统计学意义（P＜0.01或P＜0.05）,在肿块较小时即有CD4+T水平的降低和CD⁺₈T水平的升高,而CD⁺₄/CD⁺₈水平随着肿瘤负荷的增大而降低;②淋巴结转移组［LN(+)］与未转移组［LN(-)］比较,CD⁺₄/CD⁺₈在LN(+)组中显著降低(P＜0.05);CD⁺₃T、CD⁺₄T、CD⁺₈T在LN(+)组中表达增高,CD⁺₃T、CD⁺₈T的升高有统计学意义(P＜0.05或P＜0.01)。 ③CEA在LN(+)组中明显升高(P＜0.05), 在不同肿瘤负荷组间比较差异无统计学意义（P＞0.05）。CEA（+）组和CEA（-）组之间比较,CD⁺₄T升高及CD⁺₄/CD⁺₈水平降低差异均有统计学意义（P＜0.01）, 且CEA与CD⁺₄T及CD⁺₄/CD⁺₈水平密切相关（P＜0.01）。结论：乳腺癌患者存在不同程度的细胞免疫状态紊乱,且与乳腺癌CEA水平及肿块大小、淋巴结转移情况密切相关。     

Separation and expansion of tumor infiltrating lymphocytes of digestive systemin Vitro and their cytotoxicity

Summary Primary tumor tissues in the digestive system were harvested form 15 patients. By mincing, enzymatic digestion and gradient density separation, sufficient TILs (>5 × 10⁶) were obtained from 13 of 15 (88. 7 %) patientsin vitro in the presence of 500 μ/ml of recombinant interleukin-2 and 5 % fetal calf serum after one month culture. 92. 3 % (12/13) of TILs proliferated wellin vitro (92. 3%). TILs expanded from 10²-folds to 10³-folds after being cultured for one month. CD₂₅ ⁺ cell of the most fresh TILs was more than that of peripheral blood lymphocytes. CD₂₅ ⁺ cells of TILs during 4th week of the culture was significantly greater (P <0. 01) than that of fresh TILs. CD₄ ⁺/CD₈ ⁺ ratio was decreased during four culture weeks because of increase of CD₈ cells. By using modified colonmetric MTT assay for measuring activity of TILs against various tumor cells the results showed that cytotoxicity of gastro-intestinal TILs amtologous tumor cells is greater than on the other tumor cells.     

Clinical Evaluation of Integrative Chinese and Western Medicine in Treating SARS

Objective:To evaluate the effective treatment with integrative Chinese and western medi-cine(ICWM)of severe acute respiratory syndrome(SARS).Methods:The 65 cases of SARS patientswere treated with ICWM and 37 cases with western medicine(WM)alone as the control in our hospitalfrom March 11 to April 30,2003.The results were analyzed,with T subset profile tested for all the 102patients.Results:Sixty-five cases of SARS patients treated with ICWM included 52 mild cases of whom 51cases were cured(98.1％)and no patient died.As to the 13 severe cases in this group,9 were cured(69.2％)and 2 died(15.4％).Of the 37 cases treated with WM alone,16 out of the 18 common cases werecured(88.9％)and 10 out of the 19 severe cases were cured(52.6％),with the other 9 died(47.4％).Comparison between the two groups(P=0.061)showed the difference was close to the value for statisti-cal significance.But considering that the number of samples was small,more cases are needed fot furtherstudy.The immune function examination:in cases     

小鼠肝脏中细胞角蛋白19阳性细胞的肝向分化特性

目的 明确小鼠肝脏中的细胞角蛋白19 (cytokeratin 19,CK19)阳性(CK19+)细胞是否可分化为成熟肝细胞.方法 利用CK19CreERT小鼠和Rosa26-GFP小鼠杂交得到CK19CreERT/Rosa26-GFP双转基因小鼠,注射他莫昔芬(tamoxifen,TM)后检测小鼠肝脏中CK19+细胞的GFP标记情况.在此基础上分别构建3,5-二乙氧基羰基-1,4-二氢-2,4,6-三甲基吡啶和四氯化碳(carbon tetrachloride,CCl4)肝脏损伤模型,采用肝脏组织冰冻切片结合免疫荧光染色检测肝脏中GFP标记的CK19+细胞的分化情况.结果 获得CK 19CreERT/Rosa26-GFP双转基因小鼠,免疫荧光染色结果显示CK19+细胞可被GFP标记.在DDC肝脏损伤模型小鼠中检测到增生性小胆管内有GFP+细胞,这些GFP+细胞表达胆管上皮细胞标志物CK19,且DDC肝损伤模型小鼠中GFP+胆管细胞比例高于未损伤对照组[(63.5±6.3)％ vs (53.6±4.8)％,P＜0.05];在CCl4肝损伤模型组小鼠中检测到肝脏实质细胞中有GFP+细胞,这些GFP+细胞表达成熟肝细胞标志物白蛋白(ALB),且CCl4肝损伤模型组小鼠肝脏中GFP+细胞比例高于未损伤对照组[(0.15±0.02)％vs (0.008±0.003)％,P＜0.01].结论 小鼠肝脏内的CK19+细胞群体中存在具有肝向分化潜能的前体细胞,可能为真正的肝干细胞的识别鉴定提供一个新线索.     

从肺胃实热论治小儿反复呼吸道感染68例

[目的]观察清热助运法治疗小儿反复呼吸道感染肺胃积热实证的临床疗效。[方法]将68例患儿随机分为两组,分别采用清热助运中药汤剂和P-转移因子口服液治疗,疗程1个月,随访半年。[结果]治疗组总有效率为94.74%,显效率为70.05%,均明显优于对照组,差异有显著性,P<0.05;治疗后治疗组患儿血T细胞亚群CD₄、CD₈及CD₄/CD₈基本恢复正常,与对照组比较差异非常显著,P<0.01。[结论]清热助运法可明显改善患儿肺胃热盛体质。     

Changes in T cell subsets and T suppressor cell function and their relationship in human schistosomiasis japonica

Summary The function of spontaneous T suppressor cell (STs) of peripheral blood was examined in 56 patients with schistosomiasis japonica at various stages. The subsets of T cell were simultaneously phenotyped in 46 cases. The percentages of CD₃ ⁺(pan T cell), CD₄ ⁺ (helper/inducer T cell) and CD₈ ⁺ (suppressor/cytotoxic T cell) in patients with acute schistosomiasis japonica were significantly higher than those in the normal controls. In patients with chronic and advanced schistosomiasis japonica, the percentage of CD₈ ⁺ T cell and the function of STs were greatly increased, but the percentage of CD₃ ⁺ T cell and the ratio of CD₄ ⁺/CD₈ ⁺ were obviously reduced. All of these markers changed more significantly in patients with advanced schistosomiasis japonica. The percentage of CD₈ ⁺ T cell in patients with acute schistosomiasis japonica was negatively correlated with the function of STs. In patients with chronic and advanced schistosomiasis japonica the percentage of CD₈ ⁺ T cell was correlated positively and the ratio of CD₄ ⁺/CD₈ ⁺ negatively with the function of STs. The results indicated that the cellular immunity was significantly increased in cases of acute schistosomiasis japonica and decreased in those with chronic or advanced schistosomiasis japonica. The increased CD₈ ⁺ T cell may be principally cytotoxic T cells in patients with acute schistosomiasis japonica, but suppressor T cells in patients with chronic and advanced schistosomiasis japonica. The subsets of T cells and the function of T suppressor cells may play an important role in the immunoregulation of schistosomiasis japonica.     

Effect of Astragalus Injection on serious abdominal traumatic patients’ cellular immunity

Objective: To explore the change of serious abdominal traumatic patients’ cellular immunity and the effect of Astragalus Injection (AI) on it.Methods: Sixty-three serious abdominal traumatic patients were randomly assigned into two groups, the conventional group and the treated group, patients in the conventional group were given conventional treatment, while others in the treated group were given conventional treatment as the basis, with AI 20 ml was added into 250 ml of 5% glucose solution given through intravenous dripping, and then on the first day and 14th day, their T cell activated antigens as well as that of 10 healthy subjects were monitored.Results: On the first day, in the conventional group and treated group, the levels of CD ₃ ⁺ , CD ₄ ⁺ , CD ₄ ⁺ /CD ₈ ⁺ , CD ₁₆ ⁺ , CD ₆₉ ⁺ and CD ₃ ⁺ /homologous leucocytic antigen-DR (HLA-DR⁺) were apparently lower than those in the healthy group (P<0.05), while the CD ₈ ⁺ was significantly higher than that in the healthy group (P<0.05), and there was no significant difference between the conventional group and the treated group (P>0. 05); on the 14th days, the levels of CD ₃ ⁺ , CD ₄ ⁺ , CD ₄ ⁺ /CD ₈ ⁺ , CD ₁₆ ⁺ , CD ₆₉ ⁺ and CD ₃ ⁺ /HLA-DR⁺ of the treated group got closed to healthy subject value, and got even higher than those of conventional group (P<0.05); CD ₈ ⁺ got close to that of healthy subjects, while obviously lower than that of conventional group (P<0.05).Conclusion: After serious abdominal trauma, cellular immunity lowered, auxiliary use of AI was beneficial to the restoration of cellular immunity.     

Effects of tripterygii totorum in treating insulin dependent diabetes mellitus patients with islet transplantation

The authors determined both the numbers of T lymphocyte subpopulations in peripheral blood and the concentration of C-peptide in serum before experimentation at 15 days, 1 month, 6 months, and 1 year after islet transplantation in tripterygii totorum (T II) group in which 30 insulin dependent diabetes mellitus (IDDM) patients were given T II and the control group in which 24 IDDM patients did not use any immunosuppressive agents, and 20 healthy individuals were used as the healthy group. Results: Before transplantation, the numbers of T lymphocyte subpopulations such as CD₂, CD₄ in the two groups were lower than those in the healthy group (P<0.01); the ratio of CD₄/CD₈ was between 1.2 and 2.0. After transplantation the numbers of CD₂, CD₄ in the two groups were elevated, particulary in T II group the numbers of CD₂, CD₄ were close to those in the healthy group (P>0.05). The ratio of CD₄/CD₈ in the control group was more than 2.0. The average dose of insulin reduced by 84.2% in 19 patients of the T II group, 2 cases stopped insulin; the peak value of C-peptide was 3.24±1.2 ng/ml. In the control group the average dose of insulin and the peak value of C- peptide was similar to that in the T II group in the first 6 months after transplantation (P>0.05), then the dose of insulin was gradually increased and the concentration of C-peptide began decreasing. One year after transplantation both the dose of insulin and the concentration of C-peptide were close to those before transplantation. The study suggests that T II have immunosuppressive effects in islet transplantation and prolong the survival time of grafts in IDDM patients.